Rh1AG334800

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000352
Physical Location & Seq
Forward (+)
56903476 .. 56904382
907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG334800.1

Sequence Viewer

Length: 738 bp
ATGGCAGCAATGGAGTTGGGCGATACCGTTAGTCTGAAGCTCCTCATCGATGTCAAGCTTCAAAAGGTTCTCTTTGCTGAAGCCGGTAAGGATTTTGTAGATTTTCTTTTCACTCTTCTCTCTTTGCCTATTGGTACTGTCATTAGGCTTCTCTCCAAGGGTGGGATGGTTGGTAGCTTTGGTAAACTGTATGAGAGTATTGAAAATCTTGATTATACATATATCCAACCCACACTAAACAAGGATATCCTGCTCAAACCCAAGGCACCAGTTGATGGACCTAATATCCTCAAGTTGACGAACAATGTTGAATCAAGTGCTCGAAAGGAGCTGTATTTGTGTAGTCATCCAAATAATTACTCTAAATCCCACCCGTACGTGACTGAAAACCCCAATGAAAAATGTCCATCGTGTTCATACCCCATGAACCTCCGAACTACTTATGTCGGTCAGCCGACTACCACGAAAGCAACTTCTGGATATGTCAAAGACGTTGTGACATACATGATAATGGATGATCTGGAGGTGAAGCCCATGTCCACCATTTCAAGCATCACTATGCTTAACAGATTCAATATCAAGGATGTTGGGGCCCTTGTAGAGAAGGTTGTTGAACTTGGCATGGATGAGGGTGTGAAGCTATTGAAGGAGTCTCTGCAGTCCAAATCTGTCCTAACAAATGTCTTCCTTGAGAAGAAAGCTGGATTTCGTAACTTTTCATGGGAGTCGAATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

245

Amino Acids

27.28

Weight (kDa)

7.61

Isoelectric Point (pI)

26.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 9 - 233 3.9e-48 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 265
AccB7I CCANNNNNTGG 1 cut(s) 275
AcuI CTGAAG 2 cut(s) 56, 99
AfaI GTAC 2 cut(s) 136, 377
AfiI CCNNNNNNNGG 2 cut(s) 162, 275
AgsI TTSAA 7 cut(s) 62, 203, 311, 549, 574, 614, 646
AluBI AGCT 6 cut(s) 40, 58, 177, 331, 640, 701
AluI AGCT 6 cut(s) 40, 58, 177, 331, 640, 701
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 1 cut(s) 657
AoxI GGCC 1 cut(s) 591
ApaI GGGCCC 1 cut(s) 595
ApeKI GCWGC 1 cut(s) 5
AspS9I GGNCC 3 cut(s) 278, 591, 592
AsuHPI GGTGA 1 cut(s) 538
AvaII GGWCC 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 676
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 160, 269, 415
BcoDI GTCTC 1 cut(s) 657
BfmI CTRYAG 1 cut(s) 656
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 278, 591, 592
BmiI GGNNCC 3 cut(s) 267, 592, 593
BmsI GCATC 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 676
BpmI CTGGAG 1 cut(s) 542
BpuEI CTTGAG 2 cut(s) 275, 710
Bsa29I ATCGAT 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 156, 261
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 275
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 15
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 2 cut(s) 156, 261
BseGI GGATG 5 cut(s) 171, 346, 520, 589, 631
BseLI CCNNNNNNNGG 2 cut(s) 162, 275
BseMI GCAATG 1 cut(s) 15
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 595
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 593
BshNI GGYRCC 1 cut(s) 265
BshVI ATCGAT 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 322
BsiSI CCGG 1 cut(s) 84
BsiWI CGTACG 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 162, 275
BsmAI GTCTC 1 cut(s) 657
BsnI GGCC 1 cut(s) 593
Bsp120I GGGCCC 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 322, 595
Bsp143I GATC 1 cut(s) 517
BspANI GGCC 1 cut(s) 593
BspDI ATCGAT 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 267, 592, 593
BspMAI CTGCAG 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 265
BsrDI GCAATG 1 cut(s) 15
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 156, 261
BssMI GATC 1 cut(s) 517
BssT1I CCWWGG 2 cut(s) 156, 261
Bst4CI ACNGT 3 cut(s) 28, 139, 189
Bst6I CTCTTC 1 cut(s) 120
BstBAI YACGTR 1 cut(s) 379
BstF5I GGATG 5 cut(s) 171, 346, 520, 589, 631
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 657
BstMBI GATC 1 cut(s) 517
BstSFI CTRYAG 1 cut(s) 656
BstSLI GKGCMC 1 cut(s) 595
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 676
Bsu15I ATCGAT 1 cut(s) 48
BsuRI GGCC 1 cut(s) 593
BsuTUI ATCGAT 1 cut(s) 48
BtsCI GGATG 5 cut(s) 171, 346, 520, 589, 631
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 3 cut(s) 278, 591, 592
ClaI ATCGAT 1 cut(s) 48
Csp6I GTAC 2 cut(s) 135, 376
CviAII CATG 5 cut(s) 424, 505, 535, 622, 720
CviQI GTAC 2 cut(s) 135, 376
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 2 cut(s) 156, 261
Eco24I GRGCYC 1 cut(s) 595
Eco32I GATATC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 278
Eco57I CTGAAG 2 cut(s) 56, 99
EcoO109I RGGNCCY 2 cut(s) 591, 592
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 156, 261
EcoT38I GRGCYC 1 cut(s) 595
ErhI CCWWGG 2 cut(s) 156, 261
FaeI CATG 5 cut(s) 427, 508, 538, 625, 723
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 5 cut(s) 423, 504, 534, 621, 719
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 5 cut(s) 178, 333, 527, 596, 638
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 1 cut(s) 593
HapII CCGG 1 cut(s) 84
Hin1II CATG 5 cut(s) 427, 508, 538, 625, 723
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 4 cut(s) 311, 570, 650, 725
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 3 cut(s) 185, 297, 540
Hpy188I TCNGA 2 cut(s) 36, 434
Hpy188III TCNNGA 3 cut(s) 209, 477, 521
Hpy8I GTNNAC 3 cut(s) 185, 297, 540
HpyAV CCTTC 2 cut(s) 598, 640
HpyCH4III ACNGT 3 cut(s) 28, 139, 189
HpyCH4IV ACGT 2 cut(s) 378, 492
HpyCH4V TGCA 1 cut(s) 658
HpySE526I ACGT 2 cut(s) 378, 492
Hsp92II CATG 5 cut(s) 427, 508, 538, 625, 723
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 2 cut(s) 45, 328
LpnPI CCDG 6 cut(s) 97, 263, 282, 462, 506, 687
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 561
MaeII ACGT 2 cut(s) 378, 492
MaeIII GTNAC 3 cut(s) 379, 496, 710
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 3 cut(s) 107, 676, 706
MhlI GDGCHC 2 cut(s) 322, 595
MluCI AATT 1 cut(s) 355
MlyI GAGTC 2 cut(s) 659, 734
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 5 cut(s) 53, 299, 440, 517, 622
MseI TTAA 1 cut(s) 564
MslI CAYNNNNRTG 2 cut(s) 509, 557
MspI CCGG 1 cut(s) 84
NdeII GATC 1 cut(s) 517
NlaIII CATG 5 cut(s) 427, 508, 538, 625, 723
NlaIV GGNNCC 3 cut(s) 267, 592, 593
NmuCI GTSAC 2 cut(s) 379, 496
PfeI GAWTC 2 cut(s) 311, 570
Pfl23II CGTACG 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 275
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 658, 733
PpsI GAGTC 2 cut(s) 658, 733
Ppu21I YACGTR 1 cut(s) 379
PspLI CGTACG 1 cut(s) 375
PspN4I GGNNCC 3 cut(s) 267, 592, 593
PspOMI GGGCCC 1 cut(s) 591
PspPI GGNCC 3 cut(s) 278, 591, 592
PstI CTGCAG 1 cut(s) 660
RsaI GTAC 2 cut(s) 136, 377
RsaNI GTAC 2 cut(s) 135, 376
RseI CAYNNNNRTG 2 cut(s) 509, 557
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 3 cut(s) 278, 591, 592
SchI GAGTC 2 cut(s) 659, 734
SduI GDGCHC 2 cut(s) 322, 595
SfaNI GCATC 1 cut(s) 561
SfcI CTRYAG 1 cut(s) 656
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 2 cut(s) 509, 557
SmlI CTYRAG 2 cut(s) 290, 689
SmoI CTYRAG 2 cut(s) 290, 689
Sse9I AATT 1 cut(s) 355
StyI CCWWGG 2 cut(s) 156, 261
TaaI ACNGT 3 cut(s) 28, 139, 189
TaiI ACGT 2 cut(s) 381, 495
TaqI TCGA 3 cut(s) 48, 322, 728
TaqII GACCGA 1 cut(s) 437
TasI AATT 1 cut(s) 355
TfiI GAWTC 2 cut(s) 311, 570
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TseFI GTSAC 2 cut(s) 379, 496
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 379, 496
TspDTI ATGAA 4 cut(s) 405, 411, 440, 708
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.