RLG00000027409

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000352
Physical Location & Seq
Reverse (-)
11302362 .. 11303093
732 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027409

Sequence Viewer

Length: 561 bp
ATGGTTGGTAGCTTTGGGAAACTATATGAGAGTGTTGAAAATCTTGATCATACATATATCCAACCCACAATAAACAAGGATATCCTGCTCAAACCCACGGCACCAGTTACTGGACCTAATATCCTCAAATTAGCCAACAACGTTGAATCAAGTGCTCGAAAGGAGCTCTACTTGTGTAGTTGTTCAAATAGTTACACCAAAGCCCACCCGTACGTGACTGAAAACCCCAATGAAATATGTCCATCGTGTTCATACACCATGAAACTCCGAACTACTTATGTCGGTCAGCCGACTACCACGGAAGCAATATCTGGTGCCGGCGCTGTTGGATATGTCAAAGGCGTTGTGACATACATGATAATGGATGATCTGGAGGTGAAGCCCATGTCCACCATTTCAAGCATCACTATGCTTAACAGGTTTAATATCAAGGATGTTGGGGCCCTTGAAGAGAAGGTTGTTGAACTTGGCATGGATAAGGGTGTGAAGCTGTTGAAGGAGTCTCTACAGTCCAAATCTGTCCTAACAAATGTCTTCCTTGGGAAGAAGTCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

187

Amino Acids

20.28

Weight (kDa)

8.25

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 2 - 184 2.6e-28 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 100, 314
AccB7I CCANNNNNTGG 1 cut(s) 110
AclI AACGTT 1 cut(s) 141
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 1 cut(s) 110
AgsI TTSAA 7 cut(s) 38, 146, 186, 399, 449, 464, 496
AluBI AGCT 3 cut(s) 12, 166, 490
AluI AGCT 3 cut(s) 12, 166, 490
Alw21I GWGCWC 2 cut(s) 157, 168
Alw26I GTCTC 1 cut(s) 507
AlwNI CAGNNNCTG 1 cut(s) 110
AoxI GGCC 1 cut(s) 441
ApaI GGGCCC 1 cut(s) 445
ApeKI GCWGC 1 cut(s) 554
AspLEI GCGC 1 cut(s) 323
AspS9I GGNCC 3 cut(s) 113, 441, 442
AsuHPI GGTGA 1 cut(s) 388
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 445
BanI GGYRCC 2 cut(s) 100, 314
BanII GRGCYC 2 cut(s) 168, 445
BbsI GAAGAC 1 cut(s) 526
Bbv12I GWGCWC 2 cut(s) 157, 168
BbvI GCAGC 1 cut(s) 541
BccI CCATC 1 cut(s) 250
BceAI ACGGC 1 cut(s) 114
BclI TGATCA 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 507
BfmI CTRYAG 1 cut(s) 506
BfoI RGCGCY 1 cut(s) 324
BisI GCNGC 1 cut(s) 555
BlsI GCNGC 1 cut(s) 556
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 3 cut(s) 113, 441, 442
BmiI GGNNCC 4 cut(s) 102, 316, 442, 443
BmsI GCATC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 526
BpmI CTGGAG 1 cut(s) 392
BsaAI YACGTR 1 cut(s) 214
BsaJI CCNNGG 3 cut(s) 96, 297, 538
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 104, 115
BseDI CCNNGG 3 cut(s) 96, 297, 538
BseGI GGATG 2 cut(s) 370, 439
BseLI CCNNNNNNNGG 1 cut(s) 110
BseNI ACTGG 2 cut(s) 104, 115
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 1 cut(s) 541
BshFI GGCC 1 cut(s) 443
BshNI GGYRCC 2 cut(s) 100, 314
BsiHKAI GWGCWC 2 cut(s) 157, 168
BsiSI CCGG 1 cut(s) 318
BsiWI CGTACG 1 cut(s) 210
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 1 cut(s) 507
BsnI GGCC 1 cut(s) 443
Bsp120I GGGCCC 1 cut(s) 441
Bsp1286I GDGCHC 3 cut(s) 157, 168, 445
Bsp143I GATC 2 cut(s) 46, 367
BspANI GGCC 1 cut(s) 443
BspLI GGNNCC 4 cut(s) 102, 316, 442, 443
BspT107I GGYRCC 2 cut(s) 100, 314
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 2 cut(s) 104, 115
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 3 cut(s) 96, 297, 538
BssMI GATC 2 cut(s) 46, 367
BssT1I CCWWGG 1 cut(s) 538
Bst4CI ACNGT 1 cut(s) 510
Bst6I CTCTTC 1 cut(s) 444
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 319
BstDSI CCRYGG 2 cut(s) 96, 297
BstF5I GGATG 2 cut(s) 370, 439
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 2 cut(s) 49, 370
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 2 cut(s) 46, 367
BstSFI CTRYAG 1 cut(s) 506
BstSLI GKGCMC 1 cut(s) 445
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 1 cut(s) 526
BsuRI GGCC 1 cut(s) 443
BtgI CCRYGG 2 cut(s) 96, 297
BtsCI GGATG 2 cut(s) 370, 439
Cac8I GCNNGC 1 cut(s) 319
CaiI CAGNNNCTG 1 cut(s) 110
CfoI GCGC 1 cut(s) 323
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 3 cut(s) 113, 441, 442
Csp6I GTAC 1 cut(s) 211
CviAII CATG 5 cut(s) 259, 355, 385, 472, 558
CviJI RGCY 8 cut(s) 12, 134, 166, 203, 289, 382, 443, 490
CviKI_1 RGCY 8 cut(s) 12, 134, 166, 203, 289, 382, 443, 490
CviQI GTAC 1 cut(s) 211
DpnI GATC 2 cut(s) 48, 369
DpnII GATC 2 cut(s) 46, 367
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 538
Eco24I GRGCYC 2 cut(s) 168, 445
Eco32I GATATC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 166
EcoO109I RGGNCCY 2 cut(s) 441, 442
EcoRV GATATC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 538
EcoT38I GRGCYC 2 cut(s) 168, 445
ErhI CCWWGG 1 cut(s) 538
FaeI CATG 5 cut(s) 262, 358, 388, 475, 561
FatI CATG 5 cut(s) 258, 354, 384, 471, 557
FbaI TGATCA 1 cut(s) 46
Fnu4HI GCNGC 1 cut(s) 555
FokI GGATG 2 cut(s) 377, 446
FriOI GRGCYC 2 cut(s) 168, 445
Fsp4HI GCNGC 1 cut(s) 555
GlaI GCGC 1 cut(s) 322
GluI GCNGC 1 cut(s) 555
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 1 cut(s) 443
HapII CCGG 1 cut(s) 318
HhaI GCGC 1 cut(s) 323
Hin1II CATG 5 cut(s) 262, 358, 388, 475, 561
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HinfI GANTC 2 cut(s) 146, 500
HpaII CCGG 1 cut(s) 318
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 1 cut(s) 269
Hpy188III TCNNGA 2 cut(s) 44, 371
Hpy8I GTNNAC 1 cut(s) 390
HpyAV CCTTC 2 cut(s) 448, 490
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4IV ACGT 2 cut(s) 141, 213
HpyCH4V TGCA 1 cut(s) 557
HpySE526I ACGT 2 cut(s) 141, 213
Hsp92II CATG 5 cut(s) 262, 358, 388, 475, 561
HspAI GCGC 1 cut(s) 321
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 46, 367
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 7 cut(s) 96, 98, 117, 297, 331, 356, 403
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 1 cut(s) 411
MaeII ACGT 2 cut(s) 141, 213
MaeIII GTNAC 4 cut(s) 106, 191, 214, 346
MalI GATC 2 cut(s) 48, 369
MboI GATC 2 cut(s) 46, 367
MboII GAAGA 3 cut(s) 461, 526, 556
MhlI GDGCHC 3 cut(s) 157, 168, 445
MluCI AATT 1 cut(s) 128
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 2 cut(s) 85, 307
MnlI CCTC 2 cut(s) 134, 367
MroNI GCCGGC 1 cut(s) 317
MseI TTAA 2 cut(s) 414, 423
MslI CAYNNNNRTG 2 cut(s) 359, 407
MspI CCGG 1 cut(s) 318
NaeI GCCGGC 1 cut(s) 319
NdeII GATC 2 cut(s) 46, 367
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 5 cut(s) 262, 358, 388, 475, 561
NlaIV GGNNCC 4 cut(s) 102, 316, 442, 443
NmuCI GTSAC 2 cut(s) 214, 346
PdiI GCCGGC 1 cut(s) 319
PfeI GAWTC 1 cut(s) 146
Pfl23II CGTACG 1 cut(s) 210
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 1 cut(s) 556
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
Ppu21I YACGTR 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 141
PspLI CGTACG 1 cut(s) 210
PspN4I GGNNCC 4 cut(s) 102, 316, 442, 443
PspOMI GGGCCC 1 cut(s) 441
PspPI GGNCC 3 cut(s) 113, 441, 442
PstNI CAGNNNCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 2 cut(s) 359, 407
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 414, 423
SatI GCNGC 1 cut(s) 555
Sau3AI GATC 2 cut(s) 46, 367
Sau96I GGNCC 3 cut(s) 113, 441, 442
SchI GAGTC 1 cut(s) 509
SduI GDGCHC 3 cut(s) 157, 168, 445
SetI ASST 9 cut(s) 14, 118, 144, 168, 216, 378, 422, 459, 492
SfaNI GCATC 1 cut(s) 411
SfcI CTRYAG 1 cut(s) 506
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 2 cut(s) 359, 407
Sse9I AATT 1 cut(s) 128
SstI GAGCTC 1 cut(s) 168
StyI CCWWGG 1 cut(s) 538
TaaI ACNGT 1 cut(s) 510
TaiI ACGT 2 cut(s) 144, 216
TaqI TCGA 1 cut(s) 157
TaqII GACCGA 1 cut(s) 272
TasI AATT 1 cut(s) 128
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 2 cut(s) 414, 423
Tru9I TTAA 2 cut(s) 414, 423
TseFI GTSAC 2 cut(s) 214, 346
TseI GCWGC 1 cut(s) 554
Tsp45I GTSAC 2 cut(s) 214, 346
TspDTI ATGAA 3 cut(s) 240, 246, 275
TspGWI ACGGA 1 cut(s) 314
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.