Rh6BG031900

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000352
Physical Location & Seq
Forward (+)
4839637 .. 4840122
486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG031900.1

Sequence Viewer

Length: 486 bp
ATGTCCCAAAAAACTCCACCCCCGAAGGTTAGGTTGAAGCTCCTAATCGACAAAGAGTATCATAAGGTTGTGTTTGCTGAAGCTGGCAAAGAGTTCGTGGATTTCCTCTTCACTCTTCTGTCTCTGCCTCTTTTAAACGCCGCCAGAGTTTGTAAGGTTGGAAGTTTAAGCAATATCTATGGGAGCATTGACGCCCTCGGTGACAAGTACATGCTCCCCAATGTCAACAAGAACACTATGCTGAGATCCAAGGAACATGTTGCCGTATTTAACAAACTTCAGCTTGTGTTGAGCAACGAACCGGACCGTAAACCACTTATGCCGTCGAATGTGAAGAAGTTTTATATGTGTGAGAATTTCAAGTTGAAGCAACAAGGCTCATGGGTGACAGATGACTCTAAAGCCATTTGTCCAATGTGCGGTACAACTATGTCCACCGGTGTCGGCTATGTTGCTCACTTGCTCCTCCGGGAGAACCTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

161

Amino Acids

18.11

Weight (kDa)

9.37

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 145 5.5e-17 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 141, 420
AclWI GGATC 1 cut(s) 240
AcsI RAATTY 1 cut(s) 355
AcuI CTGAAG 2 cut(s) 99, 263
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 209, 424
AfiI CCNNNNNNNGG 1 cut(s) 419
AflIII ACRYGT 1 cut(s) 256
AgeI ACCGGT 1 cut(s) 437
AgsI TTSAA 3 cut(s) 37, 361, 367
AluBI AGCT 3 cut(s) 40, 83, 283
AluI AGCT 3 cut(s) 40, 83, 283
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 355
AsiGI ACCGGT 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 212, 397
AvaII GGWCC 1 cut(s) 304
BceAI ACGGC 2 cut(s) 248, 307
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 479
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 470
Bme18I GGWCC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 196, 249
BsaWI WCCGGW 2 cut(s) 301, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse118I RCCGGY 1 cut(s) 437
BseDI CCNNGG 2 cut(s) 196, 249
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMII CTCAG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 455
BshTI ACCGGT 1 cut(s) 437
BsiSI CCGG 3 cut(s) 302, 438, 469
BslI CCNNNNNNNGG 1 cut(s) 419
BsmAI GTCTC 1 cut(s) 126
Bsp143I GATC 1 cut(s) 245
BspACI CCGC 2 cut(s) 141, 420
BspCNI CTCAG 1 cut(s) 234
BspPI GGATC 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 437
BssAI RCCGGY 1 cut(s) 437
BssECI CCNNGG 2 cut(s) 196, 249
BssMI GATC 1 cut(s) 245
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 2 cut(s) 113, 120
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 1 cut(s) 242
BstKTI GATC 1 cut(s) 248
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 245
BstNSI RCATGY 2 cut(s) 214, 260
BstSCI CCNGG 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 85
Cfr10I RCCGGY 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 304
CpoI CGGWCCG 1 cut(s) 304
CseI GACGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 208, 423
CspAI ACCGGT 1 cut(s) 437
CspI CGGWCCG 1 cut(s) 304
CviAII CATG 3 cut(s) 211, 257, 381
CviJI RGCY 6 cut(s) 40, 83, 283, 378, 404, 447
CviKI_1 RGCY 6 cut(s) 40, 83, 283, 378, 404, 447
CviQI GTAC 2 cut(s) 208, 423
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 247
DpnII GATC 1 cut(s) 245
DraI TTTAAA 1 cut(s) 135
Eam1104I CTCTTC 2 cut(s) 113, 120
EarI CTCTTC 2 cut(s) 113, 120
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 304
Eco57I CTGAAG 2 cut(s) 99, 263
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 3 cut(s) 214, 260, 384
FatI CATG 3 cut(s) 210, 256, 380
Fnu4HI GCNGC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 479
GluI GCNGC 1 cut(s) 141
HapII CCGG 3 cut(s) 302, 438, 469
HgaI GACGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 3 cut(s) 214, 260, 384
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 395
HpaII CCGG 3 cut(s) 302, 438, 469
HphI GGTGA 2 cut(s) 212, 397
Hpy166II GTNNAC 3 cut(s) 226, 311, 435
Hpy8I GTNNAC 3 cut(s) 226, 311, 435
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 308
HpyF3I CTNAG 1 cut(s) 242
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 3 cut(s) 214, 260, 384
Kzo9I GATC 1 cut(s) 245
LmnI GCTCC 4 cut(s) 45, 183, 219, 468
LpnPI CCDG 5 cut(s) 69, 157, 315, 451, 482
MaeI CTAG 1 cut(s) 479
MaeIII GTNAC 2 cut(s) 200, 385
MalI GATC 1 cut(s) 247
MboI GATC 1 cut(s) 245
MboII GAAGA 3 cut(s) 100, 107, 346
MflI RGATCY 1 cut(s) 245
MluCI AATT 1 cut(s) 355
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 4 cut(s) 116, 138, 206, 476
MseI TTAA 3 cut(s) 134, 167, 270
MspI CCGG 3 cut(s) 302, 438, 469
MspR9I CCNGG 1 cut(s) 470
NciI CCSGG 1 cut(s) 470
NdeII GATC 1 cut(s) 245
NlaIII CATG 3 cut(s) 214, 260, 384
NmuCI GTSAC 2 cut(s) 200, 385
NspI RCATGY 2 cut(s) 214, 260
PciI ACATGT 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 468
PinAI ACCGGT 1 cut(s) 437
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
PscI ACATGT 1 cut(s) 256
PspPI GGNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 2 cut(s) 209, 424
RsaNI GTAC 2 cut(s) 208, 423
Rsr2I CGGWCCG 1 cut(s) 304
RsrII CGGWCCG 1 cut(s) 304
SaqAI TTAA 3 cut(s) 134, 167, 270
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 1 cut(s) 245
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 470
SetI ASST 8 cut(s) 30, 35, 42, 69, 85, 159, 285, 480
SgrAI CRCCGGYG 1 cut(s) 437
SinI GGWCC 1 cut(s) 304
Sse9I AATT 1 cut(s) 355
SsiI CCGC 2 cut(s) 141, 420
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 308
TaqI TCGA 2 cut(s) 48, 326
TasI AATT 1 cut(s) 355
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 143
Tru1I TTAA 3 cut(s) 134, 167, 270
Tru9I TTAA 3 cut(s) 134, 167, 270
TseFI GTSAC 2 cut(s) 200, 385
Tsp45I GTSAC 2 cut(s) 200, 385
VpaK11BI GGWCC 1 cut(s) 304
XapI RAATTY 1 cut(s) 355
XceI RCATGY 2 cut(s) 214, 260
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.