RLG00000027413

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000352
Physical Location & Seq
Reverse (-)
11332886 .. 11333831
946 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027413

Sequence Viewer

Length: 759 bp
ATGGCAGCAACGCCCAACTGCAGTACCGTTAGTCTGAAGCTCCTCATCGATGTGAAGCGTCAAAAGGTTTTTTTTGCTGAAGCCGGTAAGGATTTTGTGGATTTTCTTTTCACTCTTCTCTCTTTGCCTGTTGGTACTGTCATTAGGCTTCTTTCCAAGGGTGGGATGGTTGGTAGCTTTGGTAAACTATATGAGAGTGTTGAAAATCTTGATCATACATATATCCAACCCACAATAAACAAGGATATCCTGCTCAAACCCACGGCACCAGTTACTGGACCTAATATCCTCAAATTAGCCAACAACGTTGAATCAAGTGCTCGAAAGGAGCTCTACTTGTGTAGTTGTTCAAATAGTTACACCAAAGCCCACCCGTACGTGACTGAAAACCCCAATGAAAAATGTCCATCGTGTTCATACCCCATGAACCTTCGAACTACTTATGTCGGTCAGCCGACTACCACGGAAGCAACTTCTGGTGCCGGCGCTGCTGGATATGTCAAAGGCGTTGTGACATACATGATAATGGATGATCTGGAGGTGAAGCCCATGTCCACCATTTCAAGCATCACTATGCTTAACAGGTTCAATATCAAGGAAGTTGGGGCCCTTGAAGAGAAGGTTGTTGAACTTGGCATGGATAAGGGTGTGAAGCTGTTGAAGGAGTCTCTGCAGTCCAAATCTGTCCTAACAAATGTCTTCCTTGAGAAGAAAGCTGGATTTCATAACTTTTTTATGGGAGCCGAATGTTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

253

Amino Acids

27.57

Weight (kDa)

8.12

Isoelectric Point (pI)

29.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 238 3.6e-50 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 265, 479
AccB7I CCANNNNNTGG 1 cut(s) 275
AclI AACGTT 1 cut(s) 306
AcuI CTGAAG 2 cut(s) 56, 99
AfaI GTAC 3 cut(s) 25, 136, 377
AfiI CCNNNNNNNGG 2 cut(s) 162, 275
AgsI TTSAA 8 cut(s) 203, 311, 351, 564, 589, 614, 629, 661
AluBI AGCT 5 cut(s) 40, 177, 331, 655, 716
AluI AGCT 5 cut(s) 40, 177, 331, 655, 716
Alw21I GWGCWC 2 cut(s) 322, 333
Alw26I GTCTC 1 cut(s) 672
AlwNI CAGNNNCTG 1 cut(s) 275
AoxI GGCC 1 cut(s) 606
ApaI GGGCCC 1 cut(s) 610
ApeKI GCWGC 2 cut(s) 5, 488
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 3 cut(s) 278, 606, 607
AsuHPI GGTGA 1 cut(s) 553
AsuII TTCGAA 1 cut(s) 433
AvaII GGWCC 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 610
BaeI ACNNNNGTAYC 2 cut(s) 7, 40
BanI GGYRCC 2 cut(s) 265, 479
BanII GRGCYC 2 cut(s) 333, 610
BbsI GAAGAC 1 cut(s) 691
Bbv12I GWGCWC 2 cut(s) 322, 333
BbvI GCAGC 2 cut(s) 17, 475
BccI CCATC 2 cut(s) 160, 415
BceAI ACGGC 1 cut(s) 279
BclI TGATCA 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 672
BfmI CTRYAG 2 cut(s) 19, 671
BfoI RGCGCY 1 cut(s) 489
BisI GCNGC 2 cut(s) 6, 489
BlsI GCNGC 2 cut(s) 7, 490
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 278, 606, 607
BmiI GGNNCC 5 cut(s) 267, 481, 607, 608, 742
BmsI GCATC 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 691
BpmI CTGGAG 1 cut(s) 557
Bpu14I TTCGAA 1 cut(s) 433
BpuEI CTTGAG 1 cut(s) 725
Bsa29I ATCGAT 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 379
BsaJI CCNNGG 3 cut(s) 156, 261, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 275
Bse118I RCCGGY 2 cut(s) 83, 482
Bse1I ACTGG 2 cut(s) 269, 280
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 156, 261, 462
BseGI GGATG 2 cut(s) 171, 535
BseLI CCNNNNNNNGG 2 cut(s) 162, 275
BseNI ACTGG 2 cut(s) 269, 280
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 2 cut(s) 17, 475
BshFI GGCC 1 cut(s) 608
BshNI GGYRCC 2 cut(s) 265, 479
BshVI ATCGAT 1 cut(s) 48
BsiHKAI GWGCWC 2 cut(s) 322, 333
BsiSI CCGG 2 cut(s) 84, 483
BsiWI CGTACG 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 162, 275
BsmAI GTCTC 1 cut(s) 672
BsnI GGCC 1 cut(s) 608
Bsp119I TTCGAA 1 cut(s) 433
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 3 cut(s) 322, 333, 610
Bsp143I GATC 2 cut(s) 211, 532
BspANI GGCC 1 cut(s) 608
BspDI ATCGAT 1 cut(s) 48
BspLI GGNNCC 5 cut(s) 267, 481, 607, 608, 742
BspMAI CTGCAG 2 cut(s) 23, 675
BspT104I TTCGAA 1 cut(s) 433
BspT107I GGYRCC 2 cut(s) 265, 479
BsrFI RCCGGY 2 cut(s) 83, 482
BsrI ACTGG 2 cut(s) 269, 280
BssAI RCCGGY 2 cut(s) 83, 482
BssECI CCNNGG 3 cut(s) 156, 261, 462
BssMI GATC 2 cut(s) 211, 532
BssT1I CCWWGG 1 cut(s) 156
Bst4CI ACNGT 2 cut(s) 28, 139
Bst6I CTCTTC 2 cut(s) 120, 609
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 1 cut(s) 433
BstC8I GCNNGC 1 cut(s) 484
BstDSI CCRYGG 2 cut(s) 261, 462
BstF5I GGATG 2 cut(s) 171, 535
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 2 cut(s) 214, 535
BstMAI GTCTC 1 cut(s) 672
BstMBI GATC 2 cut(s) 211, 532
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstSFI CTRYAG 2 cut(s) 19, 671
BstSLI GKGCMC 1 cut(s) 610
BstV1I GCAGC 2 cut(s) 17, 475
BstV2I GAAGAC 1 cut(s) 691
Bsu15I ATCGAT 1 cut(s) 48
BsuRI GGCC 1 cut(s) 608
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 2 cut(s) 261, 462
BtsCI GGATG 2 cut(s) 171, 535
Cac8I GCNNGC 1 cut(s) 484
CaiI CAGNNNCTG 1 cut(s) 275
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 2 cut(s) 83, 482
Cfr13I GGNCC 3 cut(s) 278, 606, 607
ClaI ATCGAT 1 cut(s) 48
CseI GACGC 1 cut(s) 47
Csp6I GTAC 3 cut(s) 24, 135, 376
CviAII CATG 4 cut(s) 424, 520, 550, 637
CviQI GTAC 3 cut(s) 24, 135, 376
DpnI GATC 2 cut(s) 213, 534
DpnII GATC 2 cut(s) 211, 532
Eam1104I CTCTTC 2 cut(s) 120, 609
EarI CTCTTC 2 cut(s) 120, 609
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 156
Eco24I GRGCYC 2 cut(s) 333, 610
Eco32I GATATC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 2 cut(s) 56, 99
EcoICRI GAGCTC 1 cut(s) 331
EcoO109I RGGNCCY 2 cut(s) 606, 607
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 156
EcoT38I GRGCYC 2 cut(s) 333, 610
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 4 cut(s) 427, 523, 553, 640
FatI CATG 4 cut(s) 423, 519, 549, 636
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 2 cut(s) 6, 489
FokI GGATG 2 cut(s) 178, 542
FriOI GRGCYC 2 cut(s) 333, 610
Fsp4HI GCNGC 2 cut(s) 6, 489
GlaI GCGC 1 cut(s) 487
GluI GCNGC 2 cut(s) 6, 489
GsuI CTGGAG 1 cut(s) 557
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 1 cut(s) 608
HapII CCGG 2 cut(s) 84, 483
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 488
Hin1II CATG 4 cut(s) 427, 523, 553, 640
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HinfI GANTC 2 cut(s) 311, 665
HpaII CCGG 2 cut(s) 84, 483
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 2 cut(s) 185, 555
Hpy188I TCNGA 1 cut(s) 36
Hpy188III TCNNGA 2 cut(s) 209, 536
Hpy8I GTNNAC 2 cut(s) 185, 555
HpyAV CCTTC 3 cut(s) 440, 613, 655
HpyCH4III ACNGT 2 cut(s) 28, 139
HpyCH4IV ACGT 2 cut(s) 306, 378
HpyCH4V TGCA 2 cut(s) 21, 673
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpySE526I ACGT 2 cut(s) 306, 378
Hsp92II CATG 4 cut(s) 427, 523, 553, 640
HspAI GCGC 1 cut(s) 486
KroI GCCGGC 1 cut(s) 482
KroNI GCCGGC 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 2 cut(s) 211, 532
LmnI GCTCC 3 cut(s) 45, 328, 740
Lsp1109I GCAGC 2 cut(s) 17, 475
LweI GCATC 1 cut(s) 576
MaeII ACGT 2 cut(s) 306, 378
MaeIII GTNAC 4 cut(s) 271, 356, 379, 511
MalI GATC 2 cut(s) 213, 534
MboI GATC 2 cut(s) 211, 532
MboII GAAGA 4 cut(s) 107, 626, 691, 721
MhlI GDGCHC 3 cut(s) 322, 333, 610
MluCI AATT 1 cut(s) 293
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 3 cut(s) 53, 299, 532
MroNI GCCGGC 1 cut(s) 482
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 3 cut(s) 50, 524, 572
MspI CCGG 2 cut(s) 84, 483
MwoI GCNNNNNNNGC 1 cut(s) 488
NaeI GCCGGC 1 cut(s) 484
NdeII GATC 2 cut(s) 211, 532
NgoMIV GCCGGC 1 cut(s) 482
NlaIII CATG 4 cut(s) 427, 523, 553, 640
NlaIV GGNNCC 5 cut(s) 267, 481, 607, 608, 742
NmuCI GTSAC 2 cut(s) 379, 511
NspV TTCGAA 1 cut(s) 433
PdiI GCCGGC 1 cut(s) 484
PfeI GAWTC 1 cut(s) 311
Pfl23II CGTACG 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 275
PkrI GCNGC 2 cut(s) 7, 490
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 306
PspLI CGTACG 1 cut(s) 375
PspN4I GGNNCC 5 cut(s) 267, 481, 607, 608, 742
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 3 cut(s) 278, 606, 607
PstI CTGCAG 2 cut(s) 23, 675
PstNI CAGNNNCTG 1 cut(s) 275
RsaI GTAC 3 cut(s) 25, 136, 377
RsaNI GTAC 3 cut(s) 24, 135, 376
RseI CAYNNNNRTG 3 cut(s) 50, 524, 572
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 2 cut(s) 6, 489
Sau3AI GATC 2 cut(s) 211, 532
Sau96I GGNCC 3 cut(s) 278, 606, 607
SchI GAGTC 1 cut(s) 674
SduI GDGCHC 3 cut(s) 322, 333, 610
SfaNI GCATC 1 cut(s) 576
SfcI CTRYAG 2 cut(s) 19, 671
SfuI TTCGAA 1 cut(s) 433
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 3 cut(s) 50, 524, 572
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
Sse9I AATT 1 cut(s) 293
SstI GAGCTC 1 cut(s) 333
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 28, 139
TaiI ACGT 2 cut(s) 309, 381
TaqI TCGA 3 cut(s) 48, 322, 433
TaqII GACCGA 1 cut(s) 437
TasI AATT 1 cut(s) 293
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TseFI GTSAC 2 cut(s) 379, 511
TseI GCWGC 2 cut(s) 5, 488
Tsp45I GTSAC 2 cut(s) 379, 511
TspDTI ATGAA 4 cut(s) 405, 411, 440, 713
TspGWI ACGGA 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.