Rh1DG325300

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000352
Physical Location & Seq
Reverse (-)
55094344 .. 55096511
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG325300.1

Sequence Viewer

Length: 540 bp
ATGCCTAATAACGTAGGGTTGTTAACCAATGTCGAAAAAACTGAAAGAAAGTCGACTTGCATTTGTTCCAATACGGGAATCGTAGTTTGGCATGGCAGCTACAGTGCCTATAGCGATATCCACCACCCGTATTGTTCTGATGATCCTAATGCCACATTTCCAAAGTGCAATCACAAAATATCTACCAAAAAAAAAAAAAAGTGCAATCACAAAATGAGTATTTCTACGACTTATGTTGCTCCACCGACTGCTCCGGCTGAAGAATCTTCCGCCTCCTCTAAAAGGATAGGGATTGTCAAACCGGTTGTTACATACATGATAATGGATGACTTGGAAGTGAAGCCTATGTCTACCATCTCGACTATAGCTCTGCTCAACAGCTTTAATGTTAGAGAATTTGGTGCCCTTGAAGAGAAGGTTATTAATCTAGGCATGGATGAGGGTGTGAAATTGCTCAAGGATTCATTGCAATCAAAGTCAGTTCTTACTAATGTGTTTCTTGGAGGAGTGAAGCCTGAAGATCATAGCTCTGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

179

Amino Acids

19.58

Weight (kDa)

8.54

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 51 - 170 1.1e-16 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 2 cut(s) 53, 350
AciI CCGC 1 cut(s) 270
AclWI GGATC 1 cut(s) 137
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 2 cut(s) 279, 537
AfiI CCNNNNNNNGG 1 cut(s) 282
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 1 cut(s) 410
AjuI GAANNNNNNNTTGG 2 cut(s) 70, 102
AluBI AGCT 4 cut(s) 99, 368, 381, 528
AluI AGCT 4 cut(s) 99, 368, 381, 528
AlwI GGATC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 1 cut(s) 395
AseI ATTAAT 1 cut(s) 423
AsiGI ACCGGT 1 cut(s) 301
BaeGI GKGCMC 1 cut(s) 406
BanI GGYRCC 1 cut(s) 401
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 362
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 3 cut(s) 100, 109, 363
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 403
BpuEI CTTGAG 1 cut(s) 440
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse118I RCCGGY 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 464
BseGI GGATG 2 cut(s) 331, 442
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 1 cut(s) 464
BseRI GAGGAG 2 cut(s) 265, 519
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 1 cut(s) 108
BshNI GGYRCC 1 cut(s) 401
BshTI ACCGGT 1 cut(s) 301
BsiSI CCGG 2 cut(s) 254, 302
BslI CCNNNNNNNGG 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 2 cut(s) 142, 520
BspACI CCGC 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 301
BssMI GATC 2 cut(s) 142, 520
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 1 cut(s) 405
BstENI CCTNNNNNAGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 331, 442
BstKTI GATC 2 cut(s) 145, 523
BstMBI GATC 2 cut(s) 142, 520
BstSFI CTRYAG 3 cut(s) 100, 109, 363
BstSLI GKGCMC 1 cut(s) 406
BstV1I GCAGC 1 cut(s) 108
BtsCI GGATG 2 cut(s) 331, 442
BtsIMutI CAGTG 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 301
CspAI ACCGGT 1 cut(s) 301
CspCI CAANNNNNGTGG 2 cut(s) 113, 148
CviAII CATG 3 cut(s) 92, 316, 433
CviJI RGCY 7 cut(s) 99, 257, 343, 368, 381, 514, 528
CviKI_1 RGCY 7 cut(s) 99, 257, 343, 368, 381, 514, 528
DpnI GATC 2 cut(s) 144, 522
DpnII GATC 2 cut(s) 142, 520
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
EciI GGCGGA 1 cut(s) 259
Eco32I GATATC 1 cut(s) 118
Eco57I CTGAAG 2 cut(s) 279, 537
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 118
FaeI CATG 3 cut(s) 95, 319, 436
FaiI YATR 9 cut(s) 93, 111, 234, 313, 317, 347, 365, 434, 525
FatI CATG 3 cut(s) 91, 315, 432
FblI GTMKAC 2 cut(s) 53, 350
Fnu4HI GCNGC 1 cut(s) 97
FokI GGATG 2 cut(s) 338, 449
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 1 cut(s) 428
GluI GCNGC 1 cut(s) 97
HapII CCGG 2 cut(s) 254, 302
Hin1II CATG 3 cut(s) 95, 319, 436
HincII GTYRAC 2 cut(s) 24, 54
HindII GTYRAC 2 cut(s) 24, 54
HinfI GANTC 3 cut(s) 78, 263, 461
HpaI GTTAAC 1 cut(s) 24
HpaII CCGG 2 cut(s) 254, 302
Hpy166II GTNNAC 3 cut(s) 24, 54, 351
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 3 cut(s) 24, 54, 351
HpyAV CCTTC 1 cut(s) 409
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 5 cut(s) 60, 168, 204, 469, 533
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 3 cut(s) 95, 319, 436
KspAI GTTAAC 1 cut(s) 24
Kzo9I GATC 2 cut(s) 142, 520
LmnI GCTCC 2 cut(s) 244, 256
LpnPI CCDG 3 cut(s) 267, 315, 528
Lsp1109I GCAGC 1 cut(s) 108
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 307
MalI GATC 2 cut(s) 144, 522
MboI GATC 2 cut(s) 142, 520
MboII GAAGA 4 cut(s) 258, 272, 422, 530
MhlI GDGCHC 1 cut(s) 406
MluCI AATT 3 cut(s) 395, 449, 535
MnlI CCTC 4 cut(s) 283, 286, 433, 497
MseI TTAA 3 cut(s) 23, 384, 423
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 2 cut(s) 254, 302
NdeII GATC 2 cut(s) 142, 520
NlaIII CATG 3 cut(s) 95, 319, 436
NlaIV GGNNCC 1 cut(s) 403
PfeI GAWTC 3 cut(s) 78, 263, 461
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 320
SalI GTCGAC 1 cut(s) 52
SaqAI TTAA 3 cut(s) 23, 384, 423
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 2 cut(s) 142, 520
SduI GDGCHC 1 cut(s) 406
SetI ASST 6 cut(s) 15, 101, 370, 383, 420, 530
SfcI CTRYAG 3 cut(s) 100, 109, 363
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 3 cut(s) 395, 449, 535
SsiI CCGC 1 cut(s) 270
SspMI CTAG 1 cut(s) 428
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 15
TaqI TCGA 3 cut(s) 33, 53, 359
TasI AATT 3 cut(s) 395, 449, 535
TfiI GAWTC 3 cut(s) 78, 263, 461
Tru1I TTAA 3 cut(s) 23, 384, 423
Tru9I TTAA 3 cut(s) 23, 384, 423
TscAI CASTG 1 cut(s) 109
TseI GCWGC 1 cut(s) 96
TspDTI ATGAA 1 cut(s) 453
TspRI CASTG 1 cut(s) 109
VspI ATTAAT 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 1 cut(s) 395
XmiI GTMKAC 2 cut(s) 53, 350
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.