Rh5AG299000

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000352
Physical Location & Seq
Forward (+)
41772923 .. 41773882
960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG299000.1

Sequence Viewer

Length: 774 bp
ATGGCCGACTCTAAATCTACAGTCAGCCTGAAGCTACTCATCGATACAAAGCGTCAAAAGGTTCTGTTTGCTGAAGCAGGTAAGGACTTTGTGGATTTTCTTTTCACCCTTCTCTCTTTGCCAATTGGCACTGTTATTAGGCTTCTGTCAAAGGATGGCATGGTTGGTAGCTTTGGTAAACTGTATGACAGTGTTGAAAATCTAGATTTAACATATATGGAACCCAAACTAAACAAGGATATCCTGCTCAAACCCAAGGCACCTGTTGCTGGACCTAATATCCTCGGTTTGTTGGACTTGGCCAACGTTGACGAACCAGCTGCTAGAAATAAGGTGTTCTACATGTGTTCGAAAAGGAATAATAATATTAATAGGCGTGGACAGTGTAACTGTTCCGGCTCTGCGCCTACCGCCCACCTTTATGTGACTGAAGATCCTAACGAACAATGTCCAAAGTGCAATCACCATATGTCTTCTCAGGCGACTTATGTTGGTCAACCAAAGACCACGGTAGCAGCTTCTCGCTTCAGTGCAGCAGGATATGTCAAAGGCCTAGTTACGTACATTATAATGGATGATCTGGAGGTGAAGCCTATGTCCACTACTATTTCAACCATAACTCTGCTCAACAGGTTCAATGTTAAGGATGTTGGCACCCTCCAAGAGAAGGTTGTAGATCTTGGCATGAATGAGGGTGTGAAACTGCTGAAAGAATCCCTACAATCCAAAGCAGTTCTCACAAATGTGTTCCTGGGGAAGAAAGCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

257

Amino Acids

28.08

Weight (kDa)

9.07

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 254 1.7e-48 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
Acc36I ACCTGC 1 cut(s) 68
AccB1I GGYRCC 2 cut(s) 259, 653
AciI CCGC 1 cut(s) 411
AclI AACGTT 1 cut(s) 306
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 2 cut(s) 3, 300
AcuI CTGAAG 4 cut(s) 50, 93, 450, 511
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 2 cut(s) 269, 667
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 3 cut(s) 197, 612, 637
AjnI CCWGG 1 cut(s) 750
AleI CACNNNNGTG 1 cut(s) 743
AluBI AGCT 5 cut(s) 34, 171, 320, 518, 764
AluI AGCT 5 cut(s) 34, 171, 320, 518, 764
AlwI GGATC 1 cut(s) 428
AoxI GGCC 3 cut(s) 3, 300, 550
ApeKI GCWGC 5 cut(s) 320, 515, 533, 764, 767
AseI ATTAAT 1 cut(s) 369
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 3 cut(s) 97, 455, 598
AsuII TTCGAA 1 cut(s) 350
AvaII GGWCC 1 cut(s) 272
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 2 cut(s) 259, 653
BbsI GAAGAC 1 cut(s) 465
BbvI GCAGC 5 cut(s) 307, 527, 545, 751, 754
BccI CCATC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 1 cut(s) 752
BfaI CTAG 3 cut(s) 203, 324, 554
BfmI CTRYAG 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 68
BglII AGATCT 1 cut(s) 676
BisI GCNGC 5 cut(s) 321, 516, 534, 765, 768
BlsI GCNGC 5 cut(s) 322, 517, 535, 766, 769
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 3 cut(s) 222, 261, 655
BmrFI CCNGG 1 cut(s) 752
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 602
Bpu14I TTCGAA 1 cut(s) 350
Bsa29I ATCGAT 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 561
BsaJI CCNNGG 4 cut(s) 255, 283, 507, 751
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 667
BseBI CCWGG 1 cut(s) 752
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 4 cut(s) 255, 283, 507, 751
BseGI GGATG 3 cut(s) 160, 580, 652
BseLI CCNNNNNNNGG 2 cut(s) 269, 667
BseMII CTCAG 1 cut(s) 491
BseXI GCAGC 5 cut(s) 307, 527, 545, 751, 754
BsgI GTGCAG 1 cut(s) 552
BshFI GGCC 3 cut(s) 5, 302, 552
BshNI GGYRCC 2 cut(s) 259, 653
BshVI ATCGAT 1 cut(s) 42
BsiSI CCGG 1 cut(s) 396
BslI CCNNNNNNNGG 2 cut(s) 269, 667
BsnI GGCC 3 cut(s) 5, 302, 552
Bsp119I TTCGAA 1 cut(s) 350
Bsp143I GATC 3 cut(s) 433, 577, 676
BspACI CCGC 1 cut(s) 411
BspANI GGCC 3 cut(s) 5, 302, 552
BspCNI CTCAG 1 cut(s) 490
BspDI ATCGAT 1 cut(s) 42
BspLI GGNNCC 3 cut(s) 222, 261, 655
BspMI ACCTGC 1 cut(s) 68
BspPI GGATC 1 cut(s) 428
BspT104I TTCGAA 1 cut(s) 350
BspT107I GGYRCC 2 cut(s) 259, 653
BssECI CCNNGG 4 cut(s) 255, 283, 507, 751
BssMI GATC 3 cut(s) 433, 577, 676
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 752
Bst4CI ACNGT 7 cut(s) 22, 133, 183, 191, 384, 392, 511
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 561
BstBI TTCGAA 1 cut(s) 350
BstDEI CTNAG 1 cut(s) 477
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 3 cut(s) 160, 580, 652
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 3 cut(s) 436, 580, 679
BstMBI GATC 3 cut(s) 433, 577, 676
BstMWI GCNNNNNNNGC 2 cut(s) 266, 410
BstNI CCWGG 1 cut(s) 752
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 750
BstSFI CTRYAG 1 cut(s) 18
BstSNI TACGTA 1 cut(s) 561
BstV1I GCAGC 5 cut(s) 307, 527, 545, 751, 754
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 2 cut(s) 433, 676
BstYI RGATCY 2 cut(s) 433, 676
Bsu15I ATCGAT 1 cut(s) 42
BsuRI GGCC 3 cut(s) 5, 302, 552
BsuTUI ATCGAT 1 cut(s) 42
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 3 cut(s) 160, 580, 652
BtsIMutI CAGTG 4 cut(s) 129, 196, 389, 535
BveI ACCTGC 1 cut(s) 68
CfoI GCGC 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 1 cut(s) 42
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 562
CviAII CATG 4 cut(s) 160, 343, 685, 771
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 1 cut(s) 477
DpnI GATC 3 cut(s) 435, 579, 678
DpnII GATC 3 cut(s) 433, 577, 676
EaeI YGGCCR 2 cut(s) 3, 300
Eco105I TACGTA 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 255
Eco147I AGGCCT 1 cut(s) 552
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 4 cut(s) 50, 93, 450, 511
EcoRII CCWGG 1 cut(s) 750
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 4 cut(s) 163, 346, 688, 774
FatI CATG 4 cut(s) 159, 342, 684, 770
FauNDI CATATG 1 cut(s) 468
Fnu4HI GCNGC 5 cut(s) 321, 516, 534, 765, 768
FokI GGATG 3 cut(s) 167, 587, 659
Fsp4HI GCNGC 5 cut(s) 321, 516, 534, 765, 768
FspBI CTAG 3 cut(s) 203, 324, 554
GlaI GCGC 1 cut(s) 405
GluI GCNGC 5 cut(s) 321, 516, 534, 765, 768
GsuI CTGGAG 1 cut(s) 602
HaeIII GGCC 3 cut(s) 5, 302, 552
HapII CCGG 1 cut(s) 396
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 406
Hin1II CATG 4 cut(s) 163, 346, 688, 774
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HincII GTYRAC 2 cut(s) 310, 497
HindII GTYRAC 2 cut(s) 310, 497
HinfI GANTC 2 cut(s) 8, 713
HpaII CCGG 1 cut(s) 396
HphI GGTGA 3 cut(s) 97, 455, 598
Hpy166II GTNNAC 5 cut(s) 179, 310, 380, 497, 600
Hpy188III TCNNGA 2 cut(s) 203, 581
Hpy8I GTNNAC 5 cut(s) 179, 310, 380, 497, 600
HpyAV CCTTC 2 cut(s) 119, 661
HpyCH4III ACNGT 7 cut(s) 22, 133, 183, 191, 384, 392, 511
HpyCH4IV ACGT 2 cut(s) 306, 560
HpyCH4V TGCA 3 cut(s) 459, 533, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 410
HpyF3I CTNAG 1 cut(s) 477
HpySE526I ACGT 2 cut(s) 306, 560
Hsp92II CATG 4 cut(s) 163, 346, 688, 774
HspAI GCGC 1 cut(s) 404
Kzo9I GATC 3 cut(s) 433, 577, 676
Lsp1109I GCAGC 5 cut(s) 307, 527, 545, 751, 754
MaeI CTAG 3 cut(s) 203, 324, 554
MaeII ACGT 2 cut(s) 306, 560
MaeIII GTNAC 3 cut(s) 386, 424, 556
MalI GATC 3 cut(s) 435, 579, 678
MboI GATC 3 cut(s) 433, 577, 676
MboII GAAGA 3 cut(s) 443, 465, 769
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 2 cut(s) 433, 676
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 1 cut(s) 123
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 4 cut(s) 293, 577, 668, 685
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 3 cut(s) 209, 369, 642
MslI CAYNNNNRTG 3 cut(s) 420, 569, 743
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 1 cut(s) 752
MunI CAATTG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 752
MwoI GCNNNNNNNGC 2 cut(s) 266, 410
NdeI CATATG 1 cut(s) 468
NdeII GATC 3 cut(s) 433, 577, 676
NlaIII CATG 4 cut(s) 163, 346, 688, 774
NlaIV GGNNCC 3 cut(s) 222, 261, 655
NmuCI GTSAC 1 cut(s) 424
NspI RCATGY 1 cut(s) 346
NspV TTCGAA 1 cut(s) 350
OliI CACNNNNGTG 1 cut(s) 743
PceI AGGCCT 1 cut(s) 552
PciI ACATGT 1 cut(s) 342
PfeI GAWTC 1 cut(s) 713
PkrI GCNGC 5 cut(s) 322, 517, 535, 766, 769
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Ppu21I YACGTR 1 cut(s) 561
PscI ACATGT 1 cut(s) 342
PshBI ATTAAT 1 cut(s) 369
PsiI TTATAA 1 cut(s) 569
Psp1406I AACGTT 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 750
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 3 cut(s) 222, 261, 655
PspPI GGNCC 1 cut(s) 272
PsuI RGATCY 2 cut(s) 433, 676
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 3 cut(s) 420, 569, 743
SaqAI TTAA 3 cut(s) 209, 369, 642
SatI GCNGC 5 cut(s) 321, 516, 534, 765, 768
Sau3AI GATC 3 cut(s) 433, 577, 676
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 752
SfcI CTRYAG 1 cut(s) 18
SfuI TTCGAA 1 cut(s) 350
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 3 cut(s) 420, 569, 743
SnaBI TACGTA 1 cut(s) 561
Sse9I AATT 1 cut(s) 123
SseBI AGGCCT 1 cut(s) 552
SsiI CCGC 1 cut(s) 411
SspI AATATT 1 cut(s) 367
SspMI CTAG 3 cut(s) 203, 324, 554
StuI AGGCCT 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 7 cut(s) 22, 133, 183, 191, 384, 392, 511
TaiI ACGT 2 cut(s) 309, 563
TaqI TCGA 2 cut(s) 42, 350
TasI AATT 1 cut(s) 123
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 3 cut(s) 209, 369, 642
Tru9I TTAA 3 cut(s) 209, 369, 642
TscAI CASTG 4 cut(s) 136, 196, 389, 535
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 5 cut(s) 320, 515, 533, 764, 767
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 1 cut(s) 701
TspRI CASTG 4 cut(s) 136, 196, 389, 535
VpaK11BI GGWCC 1 cut(s) 272
VspI ATTAAT 1 cut(s) 369
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 1 cut(s) 346
XspI CTAG 3 cut(s) 203, 324, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.