Rh1DG325100

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000352
Physical Location & Seq
Forward (+)
55083477 .. 55084405
929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG325100.1

Sequence Viewer

Length: 768 bp
ATGGCAGCAACGCCCAACTCCAGTACCGTTAGTCTGAAGCTCCTCATGGATGTAAAGCGTCAAAAGGTTCTCTTTGCTGAAGCCGGTAAGGATTTTGTGGATTTTCTTTTCGCTCTTCTCTCTTTGCCTATTGGTACTGTCATTAGGCTTCTCTCCAAGGGTGGGATGGTTGGTAGCTTTGGTAAACTGTATGAGAGTATTGAAAATCTTGATAATACATATATCCAACCCACACTAAACAAGGATATCCTGCTCAAACCCAAGGCACCAGTTGATGGACCTAATATCCTCAAGTTGGCCAACAATGTTGAATCAAGTGCTCGATCGGAGCTCTATTTGTGTAGTCATTCAAATAATTACTCTAAATCCCACCCGTACGTGACTGAAAACCCCAATGAAAAATGTCCATCGTGTTCATACCCCATGAACCTCCGAACTACTTATGTCGGTCAGCCGACTACCACGGAAGCAACTTCTGGTGCCGGCGCTGCTGGATATGTCAAAGGCGTTGTGACATACATGATAATGGATGATCTGGAGGTGAAGCCCATGTCCACCATTTCAAGCATCACTATGCTTAACAGGTTCAATATCAAGGATGTTGGGGCCCTTGAAGAGAAGGTTGTTGAACTTGGCATGGATAAGGGTGTGAAGCTGTTGAAGGAGTCTCTGCGGTCCAAATCTGTCCTAACAAATGTCTTCCTTGAGAAGAAAGCTGGGATTGACATATCATTCTCGGAGGATCAACTACGTAGAGGTGGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

255

Amino Acids

27.87

Weight (kDa)

8.6

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 239 9.5e-51 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 265, 479
AccB7I CCANNNNNTGG 1 cut(s) 275
AciI CCGC 1 cut(s) 673
AclWI GGATC 1 cut(s) 750
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 56, 99
AfaI GTAC 3 cut(s) 25, 136, 377
AfiI CCNNNNNNNGG 3 cut(s) 162, 275, 295
AgsI TTSAA 8 cut(s) 203, 311, 351, 564, 589, 614, 629, 661
AluBI AGCT 5 cut(s) 40, 177, 331, 655, 716
AluI AGCT 5 cut(s) 40, 177, 331, 655, 716
Alw21I GWGCWC 2 cut(s) 322, 333
Alw26I GTCTC 1 cut(s) 672
AlwI GGATC 1 cut(s) 750
AoxI GGCC 2 cut(s) 297, 606
ApaI GGGCCC 1 cut(s) 610
ApeKI GCWGC 2 cut(s) 5, 488
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 4 cut(s) 278, 606, 607, 675
AsuHPI GGTGA 1 cut(s) 553
AvaII GGWCC 2 cut(s) 278, 675
BaeGI GKGCMC 1 cut(s) 610
BaeI ACNNNNGTAYC 2 cut(s) 7, 40
BalI TGGCCA 1 cut(s) 299
BanI GGYRCC 2 cut(s) 265, 479
BanII GRGCYC 2 cut(s) 333, 610
BbsI GAAGAC 1 cut(s) 691
Bbv12I GWGCWC 2 cut(s) 322, 333
BbvI GCAGC 2 cut(s) 17, 475
BccI CCATC 3 cut(s) 160, 269, 415
BcoDI GTCTC 1 cut(s) 672
BfoI RGCGCY 1 cut(s) 489
BisI GCNGC 2 cut(s) 6, 489
BlsI GCNGC 2 cut(s) 7, 490
Bme18I GGWCC 2 cut(s) 278, 675
BmgT120I GGNCC 4 cut(s) 278, 606, 607, 675
BmiI GGNNCC 4 cut(s) 267, 481, 607, 608
BmsI GCATC 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 691
BpmI CTGGAG 2 cut(s) 4, 557
BpuEI CTTGAG 2 cut(s) 275, 725
BsaAI YACGTR 2 cut(s) 379, 752
BsaJI CCNNGG 3 cut(s) 156, 261, 462
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 275, 295
Bse118I RCCGGY 2 cut(s) 83, 482
Bse1I ACTGG 2 cut(s) 21, 269
BseDI CCNNGG 3 cut(s) 156, 261, 462
BseGI GGATG 4 cut(s) 55, 171, 535, 604
BseLI CCNNNNNNNGG 3 cut(s) 162, 275, 295
BseNI ACTGG 2 cut(s) 21, 269
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 2 cut(s) 17, 475
BseYI CCCAGC 1 cut(s) 716
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 2 cut(s) 299, 608
BshNI GGYRCC 2 cut(s) 265, 479
BsiEI CGRYCG 1 cut(s) 326
BsiHKAI GWGCWC 2 cut(s) 322, 333
BsiSI CCGG 2 cut(s) 84, 483
BsiWI CGTACG 1 cut(s) 375
BslI CCNNNNNNNGG 3 cut(s) 162, 275, 295
BsmAI GTCTC 1 cut(s) 672
BsnI GGCC 2 cut(s) 299, 608
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 3 cut(s) 322, 333, 610
Bsp143I GATC 3 cut(s) 323, 532, 742
BspACI CCGC 1 cut(s) 673
BspANI GGCC 2 cut(s) 299, 608
BspLI GGNNCC 4 cut(s) 267, 481, 607, 608
BspPI GGATC 1 cut(s) 750
BspQI GCTCTTC 1 cut(s) 120
BspT107I GGYRCC 2 cut(s) 265, 479
BsrFI RCCGGY 2 cut(s) 83, 482
BsrI ACTGG 2 cut(s) 21, 269
BssAI RCCGGY 2 cut(s) 83, 482
BssECI CCNNGG 3 cut(s) 156, 261, 462
BssMI GATC 3 cut(s) 323, 532, 742
BssT1I CCWWGG 2 cut(s) 156, 261
Bst4CI ACNGT 3 cut(s) 28, 139, 189
Bst6I CTCTTC 2 cut(s) 120, 609
BstBAI YACGTR 2 cut(s) 379, 752
BstC8I GCNNGC 1 cut(s) 484
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 4 cut(s) 55, 171, 535, 604
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 3 cut(s) 326, 535, 745
BstMAI GTCTC 1 cut(s) 672
BstMBI GATC 3 cut(s) 323, 532, 742
BstMCI CGRYCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstSLI GKGCMC 1 cut(s) 610
BstSNI TACGTA 1 cut(s) 752
BstV1I GCAGC 2 cut(s) 17, 475
BstV2I GAAGAC 1 cut(s) 691
BsuRI GGCC 2 cut(s) 299, 608
BtgI CCRYGG 1 cut(s) 462
BtsCI GGATG 4 cut(s) 55, 171, 535, 604
Cac8I GCNNGC 1 cut(s) 484
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 2 cut(s) 83, 482
Cfr13I GGNCC 4 cut(s) 278, 606, 607, 675
CseI GACGC 1 cut(s) 47
Csp6I GTAC 3 cut(s) 24, 135, 376
CviAII CATG 5 cut(s) 46, 424, 520, 550, 637
CviQI GTAC 3 cut(s) 24, 135, 376
DpnI GATC 3 cut(s) 325, 534, 744
DpnII GATC 3 cut(s) 323, 532, 742
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 120, 609
EarI CTCTTC 2 cut(s) 120, 609
Ecl136II GAGCTC 1 cut(s) 331
Eco105I TACGTA 1 cut(s) 752
Eco130I CCWWGG 2 cut(s) 156, 261
Eco24I GRGCYC 2 cut(s) 333, 610
Eco32I GATATC 1 cut(s) 247
Eco47I GGWCC 2 cut(s) 278, 675
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 2 cut(s) 56, 99
EcoICRI GAGCTC 1 cut(s) 331
EcoO109I RGGNCCY 2 cut(s) 606, 607
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 156, 261
EcoT38I GRGCYC 2 cut(s) 333, 610
ErhI CCWWGG 2 cut(s) 156, 261
FaeI CATG 5 cut(s) 49, 427, 523, 553, 640
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 5 cut(s) 45, 423, 519, 549, 636
Fnu4HI GCNGC 2 cut(s) 6, 489
FokI GGATG 4 cut(s) 62, 178, 542, 611
FriOI GRGCYC 2 cut(s) 333, 610
Fsp4HI GCNGC 2 cut(s) 6, 489
GlaI GCGC 1 cut(s) 487
GluI GCNGC 2 cut(s) 6, 489
GsaI CCCAGC 1 cut(s) 720
GsuI CTGGAG 2 cut(s) 4, 557
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 299, 608
HapII CCGG 2 cut(s) 84, 483
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 488
Hin1II CATG 5 cut(s) 49, 427, 523, 553, 640
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HinfI GANTC 2 cut(s) 311, 665
HpaII CCGG 2 cut(s) 84, 483
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 2 cut(s) 185, 555
Hpy188I TCNGA 4 cut(s) 36, 328, 434, 739
Hpy188III TCNNGA 2 cut(s) 209, 536
Hpy8I GTNNAC 2 cut(s) 185, 555
HpyAV CCTTC 2 cut(s) 613, 655
HpyCH4III ACNGT 3 cut(s) 28, 139, 189
HpyCH4IV ACGT 2 cut(s) 378, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpySE526I ACGT 2 cut(s) 378, 751
Hsp92II CATG 5 cut(s) 49, 427, 523, 553, 640
HspAI GCGC 1 cut(s) 486
KroI GCCGGC 1 cut(s) 482
KroNI GCCGGC 1 cut(s) 484
Kzo9I GATC 3 cut(s) 323, 532, 742
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 2 cut(s) 45, 328
Lsp1109I GCAGC 2 cut(s) 17, 475
LweI GCATC 1 cut(s) 576
MaeII ACGT 2 cut(s) 378, 751
MaeIII GTNAC 2 cut(s) 379, 511
MalI GATC 3 cut(s) 325, 534, 744
MboI GATC 3 cut(s) 323, 532, 742
MboII GAAGA 4 cut(s) 107, 626, 691, 721
MhlI GDGCHC 3 cut(s) 322, 333, 610
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 1 cut(s) 355
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 6 cut(s) 53, 299, 440, 532, 733, 749
Mox20I TGGCCA 1 cut(s) 299
MroNI GCCGGC 1 cut(s) 482
MscI TGGCCA 1 cut(s) 299
MseI TTAA 2 cut(s) 579, 766
MslI CAYNNNNRTG 2 cut(s) 524, 572
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 2 cut(s) 84, 483
MwoI GCNNNNNNNGC 1 cut(s) 488
NaeI GCCGGC 1 cut(s) 484
NdeII GATC 3 cut(s) 323, 532, 742
NgoMIV GCCGGC 1 cut(s) 482
NlaIII CATG 5 cut(s) 49, 427, 523, 553, 640
NlaIV GGNNCC 4 cut(s) 267, 481, 607, 608
NmuCI GTSAC 2 cut(s) 379, 511
PciSI GCTCTTC 1 cut(s) 120
PdiI GCCGGC 1 cut(s) 484
PfeI GAWTC 1 cut(s) 311
Pfl23II CGTACG 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 275
PkrI GCNGC 2 cut(s) 7, 490
Ple19I CGATCG 1 cut(s) 326
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 2 cut(s) 379, 752
Psp124BI GAGCTC 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 716
PspLI CGTACG 1 cut(s) 375
PspN4I GGNNCC 4 cut(s) 267, 481, 607, 608
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 4 cut(s) 278, 606, 607, 675
PvuI CGATCG 1 cut(s) 326
RsaI GTAC 3 cut(s) 25, 136, 377
RsaNI GTAC 3 cut(s) 24, 135, 376
RseI CAYNNNNRTG 2 cut(s) 524, 572
SacI GAGCTC 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 579, 766
SatI GCNGC 2 cut(s) 6, 489
Sau3AI GATC 3 cut(s) 323, 532, 742
Sau96I GGNCC 4 cut(s) 278, 606, 607, 675
SchI GAGTC 1 cut(s) 674
SduI GDGCHC 3 cut(s) 322, 333, 610
SfaNI GCATC 1 cut(s) 576
SinI GGWCC 2 cut(s) 278, 675
SmiMI CAYNNNNRTG 2 cut(s) 524, 572
SmlI CTYRAG 2 cut(s) 290, 704
SmoI CTYRAG 2 cut(s) 290, 704
SnaBI TACGTA 1 cut(s) 752
Sse9I AATT 1 cut(s) 355
SsiI CCGC 1 cut(s) 673
SstI GAGCTC 1 cut(s) 333
StyI CCWWGG 2 cut(s) 156, 261
TaaI ACNGT 3 cut(s) 28, 139, 189
TaiI ACGT 2 cut(s) 381, 754
TaqI TCGA 1 cut(s) 322
TaqII GACCGA 1 cut(s) 437
TasI AATT 1 cut(s) 355
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 2 cut(s) 579, 766
Tru9I TTAA 2 cut(s) 579, 766
TseFI GTSAC 2 cut(s) 379, 511
TseI GCWGC 2 cut(s) 5, 488
Tsp45I GTSAC 2 cut(s) 379, 511
TspDTI ATGAA 3 cut(s) 405, 411, 440
TspGWI ACGGA 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 278, 675
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.