RLG00000027407

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000352
Physical Location & Seq
Forward (+)
11294114 .. 11295585
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027407

Sequence Viewer

Length: 744 bp
ATGGCAACTATTAGCTTGAAGCTACTCGTTGACACAAAGCAGAAAAAAGTTCTGTTCGCCGAAGCAGGTAAGGAATTTGTTGACTTCCTCTTCACCCTTCTTTCTTTTCCCGCCGGTACCATCATCAGGCTCCTCTCAAAGGACAACATGGTTGGAAGCCTAGGTAAACTGTACGAAAGCGTTGAAAATTTTAGTGATACATATATGCAACCCAATCTGAACAAAGACATTCTCCTGAAACCTAAAGAACCTGGTACCGTGCCTAATAACCTAGGGTTGTTAACCAATGTCAAAAAAACTGAAAGAAAGTCGACTTACATTTGTTCCAATACGGGAAACGTAGTTTGGCGTGGCAGCTACAGTGGCTATAGCGATATCCACCCGTATTGTTCTGATGATCCTAAAGCCAAATGTCCAAAGTGCAGTCACAACATGAGTATTTCAACGACTTATGTTGCTCCACCGACTGCTCCTGCGGAAGAAGCTTCTGCCGCCTCTAAAAGGATAGGGTTTGTCAAACCGGTTGTTACCTACATGATAATGGATGACTTGGAAGTGAAGCCTATGTCTACCATCTCGACCATTGCTCTGCTCAACAGCCTCAATGTTAGAGATTTTGGTGCCCTTGAAGAGAAGGTTGTTAATCTAGGCATGGATGAGGGTGTGAAATTGCTCAAGGAATCATTGCAGTCAAAGTCAGTTCTTACTAATGTGTTTCTTGGAGGAGTGAAGCATGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

248

Amino Acids

27.05

Weight (kDa)

8.73

Isoelectric Point (pI)

25.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 3 - 240 3.4e-47 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 56
Acc65I GGTACC 2 cut(s) 116, 254
AccB1I GGYRCC 3 cut(s) 116, 254, 620
AccI GTMKAC 2 cut(s) 311, 569
AciI CCGC 3 cut(s) 111, 476, 492
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 2 cut(s) 74, 187
AfaI GTAC 3 cut(s) 118, 173, 256
AfiI CCNNNNNNNGG 3 cut(s) 126, 139, 501
AgeI ACCGGT 1 cut(s) 520
AgsI TTSAA 4 cut(s) 19, 185, 444, 629
AjnI CCWGG 1 cut(s) 250
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
AluBI AGCT 4 cut(s) 15, 22, 357, 485
AluI AGCT 4 cut(s) 15, 22, 357, 485
AlwI GGATC 1 cut(s) 392
ApeKI GCWGC 1 cut(s) 354
ApoI RAATTY 2 cut(s) 74, 187
AsiGI ACCGGT 1 cut(s) 520
Asp718I GGTACC 2 cut(s) 116, 254
AspA2I CCTAGG 2 cut(s) 160, 271
AsuHPI GGTGA 1 cut(s) 85
AvrII CCTAGG 2 cut(s) 160, 271
BaeGI GKGCMC 1 cut(s) 625
BanI GGYRCC 3 cut(s) 116, 254, 620
BbvI GCAGC 1 cut(s) 366
BccI CCATC 2 cut(s) 128, 581
BciT130I CCWGG 1 cut(s) 252
BfaI CTAG 3 cut(s) 161, 272, 647
BfmI CTRYAG 2 cut(s) 358, 367
BfuAI ACCTGC 1 cut(s) 56
BisI GCNGC 2 cut(s) 355, 492
BlnI CCTAGG 2 cut(s) 160, 271
BlsI GCNGC 2 cut(s) 356, 493
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 4 cut(s) 118, 131, 256, 622
BmrFI CCNGG 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 659
BsaJI CCNNGG 2 cut(s) 160, 271
BsaWI WCCGGW 1 cut(s) 520
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 139, 501
Bse118I RCCGGY 2 cut(s) 113, 520
Bse3DI GCAATG 2 cut(s) 582, 683
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 160, 271
BseGI GGATG 2 cut(s) 550, 661
BseLI CCNNNNNNNGG 3 cut(s) 126, 139, 501
BseMI GCAATG 2 cut(s) 582, 683
BseRI GAGGAG 2 cut(s) 122, 738
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 1 cut(s) 366
BsgI GTGCAG 1 cut(s) 442
BshNI GGYRCC 3 cut(s) 116, 254, 620
BshTI ACCGGT 1 cut(s) 520
BsiSI CCGG 2 cut(s) 114, 521
BslI CCNNNNNNNGG 3 cut(s) 126, 139, 501
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 1 cut(s) 397
BspACI CCGC 3 cut(s) 111, 476, 492
BspLI GGNNCC 4 cut(s) 118, 131, 256, 622
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 1 cut(s) 392
BspT107I GGYRCC 3 cut(s) 116, 254, 620
BsrDI GCAATG 2 cut(s) 582, 683
BsrFI RCCGGY 2 cut(s) 113, 520
BssAI RCCGGY 2 cut(s) 113, 520
BssECI CCNNGG 2 cut(s) 160, 271
BssMI GATC 1 cut(s) 397
BssT1I CCWWGG 2 cut(s) 160, 271
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 171, 259, 362
Bst6I CTCTTC 2 cut(s) 95, 624
BstC8I GCNNGC 1 cut(s) 735
BstENI CCTNNNNNAGG 2 cut(s) 137, 499
BstF5I GGATG 2 cut(s) 550, 661
BstKTI GATC 1 cut(s) 400
BstMBI GATC 1 cut(s) 397
BstMWI GCNNNNNNNGC 3 cut(s) 363, 482, 491
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 737
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 2 cut(s) 358, 367
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 1 cut(s) 366
BtsCI GGATG 2 cut(s) 550, 661
BtsIMutI CAGTG 1 cut(s) 367
BveI ACCTGC 1 cut(s) 56
Cac8I GCNNGC 1 cut(s) 735
Cfr10I RCCGGY 2 cut(s) 113, 520
CsiI ACCWGGT 1 cut(s) 250
Csp6I GTAC 3 cut(s) 117, 172, 255
CspAI ACCGGT 1 cut(s) 520
CspCI CAANNNNNGTGG 2 cut(s) 368, 403
CviAII CATG 5 cut(s) 148, 433, 535, 652, 734
CviQI GTAC 3 cut(s) 117, 172, 255
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 95, 624
EarI CTCTTC 2 cut(s) 95, 624
Eco130I CCWWGG 2 cut(s) 160, 271
Eco32I GATATC 1 cut(s) 376
EcoNI CCTNNNNNAGG 2 cut(s) 137, 499
EcoRII CCWGG 1 cut(s) 250
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 160, 271
ErhI CCWWGG 2 cut(s) 160, 271
FaeI CATG 5 cut(s) 151, 436, 538, 655, 737
FatI CATG 5 cut(s) 147, 432, 534, 651, 733
FauI CCCGC 1 cut(s) 118
FblI GTMKAC 2 cut(s) 311, 569
Fnu4HI GCNGC 2 cut(s) 355, 492
FokI GGATG 2 cut(s) 557, 668
Fsp4HI GCNGC 2 cut(s) 355, 492
FspBI CTAG 3 cut(s) 161, 272, 647
GluI GCNGC 2 cut(s) 355, 492
HapII CCGG 2 cut(s) 114, 521
Hin1II CATG 5 cut(s) 151, 436, 538, 655, 737
HincII GTYRAC 4 cut(s) 31, 82, 282, 312
HindII GTYRAC 4 cut(s) 31, 82, 282, 312
HindIII AAGCTT 1 cut(s) 483
HinfI GANTC 1 cut(s) 680
HpaI GTTAAC 1 cut(s) 282
HpaII CCGG 2 cut(s) 114, 521
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 6 cut(s) 31, 82, 167, 282, 312, 570
Hpy188I TCNGA 2 cut(s) 219, 394
Hpy188III TCNNGA 2 cut(s) 235, 577
Hpy8I GTNNAC 6 cut(s) 31, 82, 167, 282, 312, 570
HpyAV CCTTC 2 cut(s) 107, 628
HpyCH4III ACNGT 3 cut(s) 171, 259, 362
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 4 cut(s) 208, 423, 688, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 363, 482, 491
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 5 cut(s) 151, 436, 538, 655, 737
KpnI GGTACC 2 cut(s) 120, 258
KspAI GTTAAC 1 cut(s) 282
Kzo9I GATC 1 cut(s) 397
LmnI GCTCC 3 cut(s) 135, 463, 475
LpnPI CCDG 8 cut(s) 51, 112, 127, 237, 248, 264, 486, 534
Lsp1109I GCAGC 1 cut(s) 366
MabI ACCWGGT 1 cut(s) 250
MaeI CTAG 3 cut(s) 161, 272, 647
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 2 cut(s) 425, 526
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 3 cut(s) 82, 491, 641
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 4 cut(s) 74, 187, 668, 739
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 6 cut(s) 98, 143, 505, 611, 652, 716
MseI TTAA 2 cut(s) 281, 642
MslI CAYNNNNRTG 1 cut(s) 539
MspI CCGG 2 cut(s) 114, 521
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 363, 482, 491
NdeII GATC 1 cut(s) 397
NlaIII CATG 5 cut(s) 151, 436, 538, 655, 737
NlaIV GGNNCC 4 cut(s) 118, 131, 256, 622
NmuCI GTSAC 1 cut(s) 425
NspI RCATGY 1 cut(s) 737
PaeI GCATGC 1 cut(s) 737
PfeI GAWTC 1 cut(s) 680
PinAI ACCGGT 1 cut(s) 520
PkrI GCNGC 2 cut(s) 356, 493
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 4 cut(s) 118, 131, 256, 622
RsaI GTAC 3 cut(s) 118, 173, 256
RsaNI GTAC 3 cut(s) 117, 172, 255
RseI CAYNNNNRTG 1 cut(s) 539
SalI GTCGAC 1 cut(s) 310
SaqAI TTAA 2 cut(s) 281, 642
SatI GCNGC 2 cut(s) 355, 492
Sau3AI GATC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 625
SexAI ACCWGGT 1 cut(s) 250
SfcI CTRYAG 2 cut(s) 358, 367
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SphI GCATGC 1 cut(s) 737
Sse9I AATT 4 cut(s) 74, 187, 668, 739
SsiI CCGC 3 cut(s) 111, 476, 492
SspMI CTAG 3 cut(s) 161, 272, 647
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 2 cut(s) 160, 271
TaaI ACNGT 3 cut(s) 171, 259, 362
TaiI ACGT 1 cut(s) 342
TaqI TCGA 2 cut(s) 311, 578
TasI AATT 4 cut(s) 74, 187, 668, 739
TauI GCSGC 1 cut(s) 494
TfiI GAWTC 1 cut(s) 680
Tru1I TTAA 2 cut(s) 281, 642
Tru9I TTAA 2 cut(s) 281, 642
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 1 cut(s) 354
Tsp45I GTSAC 1 cut(s) 425
TspRI CASTG 1 cut(s) 367
XagI CCTNNNNNAGG 2 cut(s) 137, 499
XapI RAATTY 2 cut(s) 74, 187
XceI RCATGY 1 cut(s) 737
XmaJI CCTAGG 2 cut(s) 160, 271
XmiI GTMKAC 2 cut(s) 311, 569
XspI CTAG 3 cut(s) 161, 272, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.