Rw6G003160

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000352
Physical Location & Seq
Reverse (-)
5086385 .. 5087493
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003160.1

Sequence Viewer

Length: 489 bp
ATGGTCGGAAGCCTATGCAAACTCTACGAGAGTTTCGAAAATCTCAACGGCATGTATATGCAGCAGCCTAATCTCAACAGAGACGTCCTCCTCAAACCCAAAGAACCAGCCGGTGTTCCTAATAACCTAGGTTTGTTGACCAACGTGGAATCTCGATCCATCTATCACTACATGCCCGTCCTGCCAGTACAAAATGAGTGCCAAGACGAGTTATGTGTCTCCACCCGGTACTACAAAGACAGAAGAAGGGTTGTGAAAGGGGTTGTTACATATATTATAACTGATGATTTAGAGGTGAAACCTAATGTTACCATTACCACAATTCTTCGGCTGGAGAGCTTCAATGTCAAGTGTATTTATGACCTTGAAGAGAAGGTCGTTAATGGTGTGAAGTTGCTTAAGGAATCATTGCAGTCAAAGTCAGTTCTAACCAATGTGTTTCTGAGAGGAATGACTAAATCAGATCTAAAACCAGTACTTAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

162

Amino Acids

18.58

Weight (kDa)

8.61

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 83 - 151 1.7e-08 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 278
AatII GACGTC 1 cut(s) 87
AclWI GGATC 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 3 cut(s) 189, 230, 477
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 2 cut(s) 343, 368
AluBI AGCT 1 cut(s) 339
AluI AGCT 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 75, 223
AlwI GGATC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 61, 64
AspA2I CCTAGG 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 307
AsuII TTCGAA 1 cut(s) 36
AvrII CCTAGG 1 cut(s) 127
BbvI GCAGC 2 cut(s) 73, 76
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 64
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 2 cut(s) 75, 223
BfaI CTAG 1 cut(s) 128
BfrI CTTAAG 1 cut(s) 398
BglII AGATCT 1 cut(s) 463
BisI GCNGC 2 cut(s) 62, 65
BlnI CCTAGG 1 cut(s) 127
BlsI GCNGC 2 cut(s) 63, 66
BmcAI AGTACT 1 cut(s) 477
Bme1390I CCNGG 1 cut(s) 226
BmrFI CCNGG 1 cut(s) 226
BplI GAGNNNNNCTC 2 cut(s) 72, 104
BpmI CTGGAG 1 cut(s) 353
Bpu14I TTCGAA 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 226
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 127
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 185, 473
Bse3DI GCAATG 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 127
BseMI GCAATG 1 cut(s) 407
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 2 cut(s) 185, 473
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 73, 76
BsiSI CCGG 2 cut(s) 111, 226
BsmAI GTCTC 2 cut(s) 75, 223
BsmBI CGTCTC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 36
Bsp143I GATC 2 cut(s) 155, 463
BspCNI CTCAG 1 cut(s) 435
BspPI GGATC 1 cut(s) 150
BspT104I TTCGAA 1 cut(s) 36
BspTI CTTAAG 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 2 cut(s) 185, 473
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 2 cut(s) 155, 463
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 127
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 1 cut(s) 84
BstAFI CTTAAG 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 36
BstDEI CTNAG 1 cut(s) 443
BstKTI GATC 2 cut(s) 158, 466
BstMAI GTCTC 2 cut(s) 75, 223
BstMBI GATC 2 cut(s) 155, 463
BstMWI GCNNNNNNNGC 1 cut(s) 181
BstNSI RCATGY 2 cut(s) 55, 175
BstSCI CCNGG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 73, 76
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 110
Csp6I GTAC 3 cut(s) 188, 229, 476
CviAII CATG 2 cut(s) 52, 172
CviJI RGCY 5 cut(s) 12, 67, 110, 331, 339
CviKI_1 RGCY 5 cut(s) 12, 67, 110, 331, 339
CviQI GTAC 3 cut(s) 188, 229, 476
DdeI CTNAG 1 cut(s) 443
DpnI GATC 2 cut(s) 157, 465
DpnII GATC 2 cut(s) 155, 463
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Eco130I CCWWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 2 cut(s) 55, 175
FatI CATG 2 cut(s) 51, 171
Fnu4HI GCNGC 2 cut(s) 62, 65
Fsp4HI GCNGC 2 cut(s) 62, 65
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 62, 65
GsuI CTGGAG 1 cut(s) 353
HapII CCGG 2 cut(s) 111, 226
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 2 cut(s) 55, 175
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 2 cut(s) 149, 404
HpaII CCGG 2 cut(s) 111, 226
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 3 cut(s) 8, 444, 463
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 1 cut(s) 138
HpyAV CCTTC 2 cut(s) 240, 367
HpyCH4IV ACGT 2 cut(s) 84, 144
HpyCH4V TGCA 3 cut(s) 18, 61, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 181
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 2 cut(s) 84, 144
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 2 cut(s) 55, 175
Kzo9I GATC 2 cut(s) 155, 463
LpnPI CCDG 6 cut(s) 120, 124, 194, 198, 239, 317
Lsp1109I GCAGC 2 cut(s) 73, 76
MaeI CTAG 1 cut(s) 128
MaeII ACGT 2 cut(s) 84, 144
MaeIII GTNAC 2 cut(s) 265, 307
MalI GATC 2 cut(s) 157, 465
MboI GATC 2 cut(s) 155, 463
MboII GAAGA 3 cut(s) 255, 317, 380
MflI RGATCY 1 cut(s) 463
MluCI AATT 1 cut(s) 321
MnlI CCTC 4 cut(s) 98, 101, 286, 440
MseI TTAA 3 cut(s) 381, 399, 480
MslI CAYNNNNRTG 1 cut(s) 56
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 2 cut(s) 111, 226
MspR9I CCNGG 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 181
NciI CCSGG 1 cut(s) 226
NdeII GATC 2 cut(s) 155, 463
NlaIII CATG 2 cut(s) 55, 175
NspI RCATGY 2 cut(s) 55, 175
NspV TTCGAA 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 2 cut(s) 149, 404
PkrI GCNGC 2 cut(s) 63, 66
PsiI TTATAA 1 cut(s) 278
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 3 cut(s) 189, 230, 477
RsaNI GTAC 3 cut(s) 188, 229, 476
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 3 cut(s) 381, 399, 480
SatI GCNGC 2 cut(s) 62, 65
Sau3AI GATC 2 cut(s) 155, 463
ScaI AGTACT 1 cut(s) 477
ScrFI CCNGG 1 cut(s) 226
SetI ASST 9 cut(s) 87, 129, 133, 147, 297, 304, 341, 366, 378
SfuI TTCGAA 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 1 cut(s) 321
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 1 cut(s) 127
TaiI ACGT 2 cut(s) 87, 147
TaqI TCGA 2 cut(s) 36, 154
TasI AATT 1 cut(s) 321
TatI WGTACW 2 cut(s) 187, 475
TfiI GAWTC 2 cut(s) 149, 404
Tru1I TTAA 3 cut(s) 381, 399, 480
Tru9I TTAA 3 cut(s) 381, 399, 480
TseI GCWGC 2 cut(s) 61, 64
Vha464I CTTAAG 1 cut(s) 398
XceI RCATGY 2 cut(s) 55, 175
XmaJI CCTAGG 1 cut(s) 127
XspI CTAG 1 cut(s) 128
ZraI GACGTC 1 cut(s) 85
ZrmI AGTACT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.