Rh6CG033200

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000352
Physical Location & Seq
Forward (+)
3115521 .. 3116333
813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG033200.1

Sequence Viewer

Length: 666 bp
ATGTCCGCCCCCGAAGCTGGCAAAGAGTTCGTGGATTTCCTCTTCACTCTTCTGTCTTTGCCTCTTTTCAACGCGGCCAGAGTTTGTAAGGTTGGAAGTTTAAGCAATATCTATGGGAGCATTGACGCCCTCGGTGTCAAGTACATGCTCCCCAATGTCAACAAGAACACTATGCTGAGATCCAAGGAATATGTTGCTGTATTTAACAAACTTCAGCTTGTGTTGAGCAACGAACCGGACCTTAAACCAATTATGCCGTCGAATGTGAAGAAGTTTTATATGTGTGAGAATTTCAAGTTGAAGCAACACGGCTCATGGGTGACAGATGACTCTAAAGCCATTTGTCCAATGTGCAGTACAACTATGTCGACCGGTGTCGGCTATGTTGCTCCTCCGGGAGAACCTAGTTTGATGACCAGGGACGAAGGGTATGTGAGAGGTGTTGCTTCATACATGATAATGGACGATCTGAGAGTCAAACCTTTGTCTGTCTTCTCAAATTTGAATTTGCTTAAGAAGTGCATCAATGGCAGTAGTGGTTTTAATAACCTGGAAGAGAAGGTGGTTCATGTTGAAATGGATCAGGTAGAGAAATGGTTGAAGGCATTAATGGAGTCAAACTCAGTTCTTACCGATCTCTTCCCTGGGAAGAAACCAATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

221

Amino Acids

24.55

Weight (kDa)

8.5

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 214 3.6e-23 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 368
AccII CGCG 1 cut(s) 74
AciI CCGC 2 cut(s) 6, 74
AclWI GGATC 2 cut(s) 174, 588
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 3 cut(s) 289, 499, 505
AcuI CTGAAG 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 2 cut(s) 143, 358
AfiI CCNNNNNNNGG 1 cut(s) 17
AflII CTTAAG 1 cut(s) 512
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 6 cut(s) 70, 295, 301, 505, 575, 601
AjnI CCWGG 3 cut(s) 416, 549, 643
AluBI AGCT 2 cut(s) 17, 217
AluI AGCT 2 cut(s) 17, 217
AlwI GGATC 2 cut(s) 174, 588
AoxI GGCC 1 cut(s) 75
ApoI RAATTY 3 cut(s) 289, 499, 505
AseI ATTAAT 1 cut(s) 608
AsiGI ACCGGT 1 cut(s) 371
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 484
BceAI ACGGC 2 cut(s) 241, 325
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 3 cut(s) 418, 551, 645
BcnI CCSGG 1 cut(s) 396
BfaI CTAG 1 cut(s) 405
BfrI CTTAAG 1 cut(s) 512
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 4 cut(s) 396, 418, 551, 645
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 238
BmrFI CCNGG 4 cut(s) 396, 418, 551, 645
BmsI GCATC 1 cut(s) 531
BoxI GACNNNNGTC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 484
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpuMI CCSGG 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 5 cut(s) 130, 183, 417, 643, 644
BsaWI WCCGGW 2 cut(s) 235, 371
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse118I RCCGGY 1 cut(s) 371
BseBI CCWGG 3 cut(s) 418, 551, 645
BseDI CCNNGG 5 cut(s) 130, 183, 417, 643, 644
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 3 cut(s) 167, 461, 636
BseRI GAGGAG 1 cut(s) 381
BsgI GTGCAG 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 371
BsiEI CGRYCG 1 cut(s) 372
BsiSI CCGG 3 cut(s) 236, 372, 395
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 1 cut(s) 77
Bsp143I GATC 4 cut(s) 179, 466, 580, 634
BspACI CCGC 2 cut(s) 6, 74
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 3 cut(s) 168, 462, 635
BspFNI CGCG 1 cut(s) 74
BspPI GGATC 2 cut(s) 174, 588
BspTI CTTAAG 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 371
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 5 cut(s) 130, 183, 417, 643, 644
BssMI GATC 4 cut(s) 179, 466, 580, 634
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 3 cut(s) 418, 551, 645
Bst6I CTCTTC 4 cut(s) 47, 54, 549, 644
BstACI GRCGYC 1 cut(s) 126
BstAFI CTTAAG 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 3 cut(s) 176, 470, 622
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 4 cut(s) 182, 469, 583, 637
BstMBI GATC 4 cut(s) 179, 466, 580, 634
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 2 cut(s) 14, 528
BstNI CCWGG 3 cut(s) 418, 551, 645
BstNSI RCATGY 1 cut(s) 148
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 4 cut(s) 394, 416, 549, 643
BstUI CGCG 1 cut(s) 74
BstV2I GAAGAC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BsuRI GGCC 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 19
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 1 cut(s) 238
CseI GACGC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 142, 357
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 4 cut(s) 145, 315, 454, 569
CviJI RGCY 6 cut(s) 17, 77, 217, 312, 338, 381
CviKI_1 RGCY 6 cut(s) 17, 77, 217, 312, 338, 381
CviQI GTAC 2 cut(s) 142, 357
DdeI CTNAG 3 cut(s) 176, 470, 622
DpnI GATC 4 cut(s) 181, 468, 582, 636
DpnII GATC 4 cut(s) 179, 466, 580, 634
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 4 cut(s) 47, 54, 549, 644
EarI CTCTTC 4 cut(s) 47, 54, 549, 644
Eco130I CCWWGG 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 416, 549, 643
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 4 cut(s) 148, 318, 457, 572
FaqI GGGAC 1 cut(s) 434
FatI CATG 4 cut(s) 144, 314, 453, 568
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 75
FspBI CTAG 1 cut(s) 405
GluI GCNGC 1 cut(s) 75
HaeIII GGCC 1 cut(s) 77
HapII CCGG 3 cut(s) 236, 372, 395
HgaI GACGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 4 cut(s) 148, 318, 457, 572
HincII GTYRAC 2 cut(s) 160, 369
HindII GTYRAC 2 cut(s) 160, 369
HinfI GANTC 3 cut(s) 329, 474, 614
HpaII CCGG 3 cut(s) 236, 372, 395
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 2 cut(s) 160, 369
Hpy188I TCNGA 1 cut(s) 471
Hpy8I GTNNAC 2 cut(s) 160, 369
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 3 cut(s) 419, 553, 595
HpyCH4V TGCA 2 cut(s) 354, 522
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 528
HpyF3I CTNAG 3 cut(s) 176, 470, 622
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 4 cut(s) 148, 318, 457, 572
Kzo9I GATC 4 cut(s) 179, 466, 580, 634
LmnI GCTCC 3 cut(s) 117, 153, 394
LweI GCATC 1 cut(s) 531
MaeI CTAG 1 cut(s) 405
MaeIII GTNAC 1 cut(s) 319
MalI GATC 4 cut(s) 181, 468, 582, 636
MboI GATC 4 cut(s) 179, 466, 580, 634
MboII GAAGA 7 cut(s) 34, 41, 280, 484, 566, 631, 661
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 1 cut(s) 179
MluCI AATT 5 cut(s) 249, 289, 499, 505, 657
MlyI GAGTC 3 cut(s) 323, 483, 623
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 5 cut(s) 50, 72, 140, 402, 431
MseI TTAA 6 cut(s) 101, 204, 243, 513, 543, 608
MslI CAYNNNNRTG 1 cut(s) 458
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 3 cut(s) 236, 372, 395
MspR9I CCNGG 4 cut(s) 396, 418, 551, 645
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 3 cut(s) 418, 551, 645
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 2 cut(s) 14, 528
NciI CCSGG 1 cut(s) 396
NdeII GATC 4 cut(s) 179, 466, 580, 634
NlaIII CATG 4 cut(s) 148, 318, 457, 572
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 1 cut(s) 148
PasI CCCWGGG 1 cut(s) 644
PfoI TCCNGGA 1 cut(s) 394
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 3 cut(s) 323, 482, 622
PpsI GAGTC 3 cut(s) 323, 482, 622
PshAI GACNNNNGTC 1 cut(s) 374
PshBI ATTAAT 1 cut(s) 608
Psp6I CCWGG 3 cut(s) 416, 549, 643
PspGI CCWGG 3 cut(s) 416, 549, 643
PspPI GGNCC 1 cut(s) 238
PsuI RGATCY 1 cut(s) 179
RsaI GTAC 2 cut(s) 143, 358
RsaNI GTAC 2 cut(s) 142, 357
RseI CAYNNNNRTG 1 cut(s) 458
SalI GTCGAC 1 cut(s) 367
SaqAI TTAA 6 cut(s) 101, 204, 243, 513, 543, 608
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 4 cut(s) 179, 466, 580, 634
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 3 cut(s) 323, 483, 623
ScrFI CCNGG 4 cut(s) 396, 418, 551, 645
SfaNI GCATC 1 cut(s) 531
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 5 cut(s) 249, 289, 499, 505, 657
SsiI CCGC 2 cut(s) 6, 74
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 4 cut(s) 394, 416, 549, 643
StyI CCWWGG 1 cut(s) 183
TaqI TCGA 2 cut(s) 260, 368
TasI AATT 5 cut(s) 249, 289, 499, 505, 657
TatI WGTACW 2 cut(s) 141, 356
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 6 cut(s) 101, 204, 243, 513, 543, 608
Tru9I TTAA 6 cut(s) 101, 204, 243, 513, 543, 608
TseFI GTSAC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 438, 557
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 238
VspI ATTAAT 1 cut(s) 608
XapI RAATTY 3 cut(s) 289, 499, 505
XceI RCATGY 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 368
XspI CTAG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.