Rorug01G0323200

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000352
Physical Location & Seq
Forward (+)
44204502 .. 44205704
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0323200.1

Sequence Viewer

Length: 1203 bp
ATGGAATCAGAGAATACACAACATTCCCAGATTGTCGAGATCCATGGAGATGTTCCTGGTGAAGAGACGAGTGTGGGTGGGAGTAAAATTTGTGGGGAAGCACCGTGTGGACTGTCGGATGTTGGACCAAATTCTAAAGATTCCAAAGAACGATCAGCTGCCATGCGCAAGCTCTTGATAGCAGTGGTACTCTGTGTGCTCTTCATGGGTGTTGAAGTGTTTGGTGGCATTGAAGCCAATAGCTTAGCAATATTGACTGATGCAGCACATTTGCTCTCTGATGTTGCAGCCTTTGCCATCTCCTTGTTCTCCTTGTGGGCTGCTAGCTGGGAAACCACTCCCCGTCAGTCTTACGGCTTTTTCAGGATTGAGATTCTCGGTGCTTTGGTTTCTATCCAACTCATATGGTTGCTTGCTGGGATATTGGTGTATGAAGCCATTGTTAGAATGATTAATAACACATCTGAGGTGAATGGTTTTCTGATGTTTCTTGTTGCTGCATTTGGTCTAGTGGTTAATATTGGCATGGCTGTACTGTTAGGTCATGATCATGGCCATGGCCATGGACATGATGATCATGATCATCACAGCCATGGAATGACATTTTCTACTCATCATCACCATGAGGAACACTCTACTGATGAAGAACACTCGCATGATGAACACCATCACAGTCATGATGATCATAAACACAATGGTGCTGATGAAGGTCATGCTCATGAGCCACTCTTGGACAAGCCAGAAGACGGGCATGGCCAGAAGAAGCAACGGAACATAAATGTGCAGGGAGCTTACCTTCATGTTCTTGGGGACTTGGTTCAAAGTATTGGTGTAATGATTGGTGGGGCCATCATATGGTACAAGCCAGAATGGAAGATAGTTGATTTGATTTGCACCCTAGTATTTTCTGTCATTGTTTTGGGGACAACTTTCAGGATGATGAGGAACATACTCGATGTACTAATGGAGAGCACACCAAGAGAGATTGATGCAACAAAACTTGAGAAGGGGCTGTTGGAGATGGAAGAAGTGGTGGAAATCCATGAGCTGCATATATGGGCAATCACAGTTGGGAAAGTCCTGCTGGCTTGCCATGTGAAAATCAGACCTGAAGCAAATGCAGACATGGTGCTGGACAAAGTGATTGATTATATCAGAAGGGAGTACAACATCAGCCATGTGACTATACAAGTTGAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

400

Amino Acids

44.38

Weight (kDa)

5.51

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 58 - 322 5e-42 Cation efflux transmembrane domain
ZT_dimer PF16916 326 - 399 8.7e-13 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 855
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 3 cut(s) 553, 559, 754
AcsI RAATTY 2 cut(s) 87, 130
AcuI CTGAAG 1 cut(s) 1131
AdeI CACNNNGTG 1 cut(s) 107
AfaI GTAC 5 cut(s) 189, 534, 860, 960, 1166
AfiI CCNNNNNNNGG 2 cut(s) 746, 855
AgsI TTSAA 3 cut(s) 215, 233, 821
AjnI CCWGG 1 cut(s) 55
AjuI GAANNNNNNNTTGG 2 cut(s) 998, 1030
AleI CACNNNNGTG 1 cut(s) 696
AluBI AGCT 6 cut(s) 158, 172, 243, 327, 791, 1048
AluI AGCT 6 cut(s) 158, 172, 243, 327, 791, 1048
Alw21I GWGCWC 2 cut(s) 201, 974
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 1 cut(s) 34
AoxI GGCC 4 cut(s) 553, 559, 754, 846
ApeKI GCWGC 6 cut(s) 158, 263, 287, 320, 497, 1048
ApoI RAATTY 2 cut(s) 87, 130
AseI ATTAAT 1 cut(s) 453
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 2 cut(s) 125, 846
AsuHPI GGTGA 3 cut(s) 71, 481, 611
AsuNHI GCTAGC 1 cut(s) 323
AvaII GGWCC 1 cut(s) 125
BalI TGGCCA 3 cut(s) 555, 561, 756
BbsI GAAGAC 1 cut(s) 750
Bbv12I GWGCWC 2 cut(s) 201, 974
BbvI GCAGC 6 cut(s) 145, 275, 299, 307, 484, 1035
BccI CCATC 4 cut(s) 305, 675, 857, 1015
BceAI ACGGC 1 cut(s) 370
BciT130I CCWGG 1 cut(s) 57
BclI TGATCA 4 cut(s) 547, 574, 580, 682
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 3 cut(s) 324, 509, 899
BisI GCNGC 6 cut(s) 159, 264, 288, 321, 498, 1049
BlpI GCTNAGC 1 cut(s) 244
BlsI GCNGC 6 cut(s) 160, 265, 289, 322, 499, 1050
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 2 cut(s) 125, 846
BmiI GGNNCC 1 cut(s) 847
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 2 cut(s) 250, 979
BmtI GCTAGC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 750
BplI GAGNNNNNCTC 2 cut(s) 617, 649
Bpu1102I GCTNAGC 1 cut(s) 244
BpuEI CTTGAG 1 cut(s) 1022
BsaBI GATNNNNATC 1 cut(s) 579
BsaJI CCNNGG 4 cut(s) 43, 556, 562, 592
Bsc4I CCNNNNNNNGG 2 cut(s) 746, 855
Bse8I GATNNNNATC 1 cut(s) 579
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 4 cut(s) 43, 556, 562, 592
BseGI GGATG 2 cut(s) 124, 942
BseJI GATNNNNATC 1 cut(s) 579
BseLI CCNNNNNNNGG 2 cut(s) 746, 855
BseMII CTCAG 1 cut(s) 456
BseXI GCAGC 6 cut(s) 145, 275, 299, 307, 484, 1035
BseYI CCCAGC 2 cut(s) 327, 416
BsgI GTGCAG 1 cut(s) 803
BshFI GGCC 4 cut(s) 555, 561, 756, 848
BsiHKAI GWGCWC 2 cut(s) 201, 974
BslFI GGGAC 2 cut(s) 824, 937
BslI CCNNNNNNNGG 2 cut(s) 746, 855
BsmAI GTCTC 1 cut(s) 59
BsmBI CGTCTC 1 cut(s) 59
BsmFI GGGAC 2 cut(s) 824, 937
BsnI GGCC 4 cut(s) 555, 561, 756, 848
Bsp1286I GDGCHC 2 cut(s) 201, 974
Bsp143I GATC 6 cut(s) 39, 152, 547, 574, 580, 682
Bsp1720I GCTNAGC 1 cut(s) 244
Bsp19I CCATGG 4 cut(s) 43, 556, 562, 592
BspANI GGCC 4 cut(s) 555, 561, 756, 848
BspCNI CTCAG 1 cut(s) 457
BspHI TCATGA 4 cut(s) 544, 577, 676, 718
BspLI GGNNCC 1 cut(s) 847
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 1 cut(s) 34
BspQI GCTCTTC 1 cut(s) 206
BssECI CCNNGG 4 cut(s) 43, 556, 562, 592
BssMI GATC 6 cut(s) 39, 152, 547, 574, 580, 682
BssT1I CCWWGG 4 cut(s) 43, 556, 562, 592
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 5 cut(s) 105, 114, 537, 674, 1069
Bst6I CTCTTC 2 cut(s) 57, 206
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 5 cut(s) 170, 325, 414, 1086, 1090
BstDEI CTNAG 2 cut(s) 244, 465
BstDSI CCRYGG 4 cut(s) 43, 556, 562, 592
BstF5I GGATG 2 cut(s) 124, 942
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 6 cut(s) 42, 155, 550, 577, 583, 685
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 6 cut(s) 39, 152, 547, 574, 580, 682
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 55
BstV1I GCAGC 6 cut(s) 145, 275, 299, 307, 484, 1035
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 1 cut(s) 39
BstXI CCANNNNNNTGG 1 cut(s) 563
BstYI RGATCY 1 cut(s) 39
BsuRI GGCC 4 cut(s) 555, 561, 756, 848
BtgI CCRYGG 4 cut(s) 43, 556, 562, 592
BtsCI GGATG 2 cut(s) 124, 942
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 5 cut(s) 170, 325, 414, 1086, 1090
CciI TCATGA 4 cut(s) 544, 577, 676, 718
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 125, 846
Csp6I GTAC 5 cut(s) 188, 533, 859, 959, 1165
CviQI GTAC 5 cut(s) 188, 533, 859, 959, 1165
DdeI CTNAG 2 cut(s) 244, 465
DpnI GATC 6 cut(s) 41, 154, 549, 576, 582, 684
DpnII GATC 6 cut(s) 39, 152, 547, 574, 580, 682
DraIII CACNNNGTG 1 cut(s) 107
EaeI YGGCCR 3 cut(s) 553, 559, 754
Eam1104I CTCTTC 2 cut(s) 57, 206
EarI CTCTTC 2 cut(s) 57, 206
Eco130I CCWWGG 4 cut(s) 43, 556, 562, 592
Eco47I GGWCC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 1131
EcoRII CCWGG 1 cut(s) 55
EcoT14I CCWWGG 4 cut(s) 43, 556, 562, 592
ErhI CCWWGG 4 cut(s) 43, 556, 562, 592
Esp3I CGTCTC 1 cut(s) 59
FaqI GGGAC 2 cut(s) 824, 937
FauNDI CATATG 2 cut(s) 404, 854
FbaI TGATCA 4 cut(s) 547, 574, 580, 682
Fnu4HI GCNGC 6 cut(s) 159, 264, 288, 321, 498, 1049
FokI GGATG 2 cut(s) 131, 949
Fsp4HI GCNGC 6 cut(s) 159, 264, 288, 321, 498, 1049
FspBI CTAG 3 cut(s) 324, 509, 899
FspI TGCGCA 1 cut(s) 167
GlaI GCGC 1 cut(s) 167
GluI GCNGC 6 cut(s) 159, 264, 288, 321, 498, 1049
GsaI CCCAGC 2 cut(s) 331, 420
HaeIII GGCC 4 cut(s) 555, 561, 756, 848
HhaI GCGC 1 cut(s) 168
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HinfI GANTC 3 cut(s) 5, 140, 373
HphI GGTGA 3 cut(s) 71, 481, 611
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 7 cut(s) 10, 118, 280, 466, 483, 1106, 1157
Hpy188III TCNNGA 8 cut(s) 37, 175, 364, 545, 578, 677, 719, 934
Hpy8I GTNNAC 1 cut(s) 110
HpyAV CCTTC 4 cut(s) 701, 806, 1000, 1152
HpyCH4III ACNGT 5 cut(s) 105, 114, 537, 674, 1069
HpyCH4V TGCA 8 cut(s) 263, 287, 500, 784, 894, 992, 1051, 1121
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 2 cut(s) 244, 465
HspAI GCGC 1 cut(s) 166
Ksp22I TGATCA 4 cut(s) 547, 574, 580, 682
Kzo9I GATC 6 cut(s) 39, 152, 547, 574, 580, 682
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 1 cut(s) 788
Lsp1109I GCAGC 6 cut(s) 145, 275, 299, 307, 484, 1035
LweI GCATC 2 cut(s) 250, 979
MaeI CTAG 3 cut(s) 324, 509, 899
MaeIII GTNAC 1 cut(s) 1180
MalI GATC 6 cut(s) 41, 154, 549, 576, 582, 684
MboI GATC 6 cut(s) 39, 152, 547, 574, 580, 682
MboII GAAGA 7 cut(s) 74, 193, 656, 755, 772, 886, 1037
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 2 cut(s) 201, 974
MlsI TGGCCA 3 cut(s) 555, 561, 756
MluCI AATT 2 cut(s) 87, 130
MluNI TGGCCA 3 cut(s) 555, 561, 756
MmeI TCCRAC 4 cut(s) 96, 103, 421, 996
MnlI CCTC 3 cut(s) 460, 619, 936
Mox20I TGGCCA 3 cut(s) 555, 561, 756
MscI TGGCCA 3 cut(s) 555, 561, 756
MseI TTAA 3 cut(s) 453, 516, 1201
Msp20I TGGCCA 3 cut(s) 555, 561, 756
MspA1I CMGCKG 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 293
NcoI CCATGG 4 cut(s) 43, 556, 562, 592
NdeI CATATG 2 cut(s) 404, 854
NdeII GATC 6 cut(s) 39, 152, 547, 574, 580, 682
NheI GCTAGC 1 cut(s) 323
NlaIV GGNNCC 1 cut(s) 847
NmuCI GTSAC 1 cut(s) 1180
NsbI TGCGCA 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 696
PagI TCATGA 4 cut(s) 544, 577, 676, 718
PciSI GCTCTTC 1 cut(s) 206
PfeI GAWTC 3 cut(s) 5, 140, 373
PflMI CCANNNNNTGG 1 cut(s) 855
PkrI GCNGC 6 cut(s) 160, 265, 289, 322, 499, 1050
PshBI ATTAAT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 55
PspFI CCCAGC 2 cut(s) 327, 416
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 847
PspPI GGNCC 2 cut(s) 125, 846
PsuI RGATCY 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 5 cut(s) 189, 534, 860, 960, 1166
RsaNI GTAC 5 cut(s) 188, 533, 859, 959, 1165
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 3 cut(s) 453, 516, 1201
SatI GCNGC 6 cut(s) 159, 264, 288, 321, 498, 1049
Sau3AI GATC 6 cut(s) 39, 152, 547, 574, 580, 682
Sau96I GGNCC 2 cut(s) 125, 846
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 2 cut(s) 201, 974
SfaNI GCATC 2 cut(s) 250, 979
SinI GGWCC 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
Sse9I AATT 2 cut(s) 87, 130
SspI AATATT 2 cut(s) 252, 520
SspMI CTAG 3 cut(s) 324, 509, 899
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 4 cut(s) 43, 556, 562, 592
TaaI ACNGT 5 cut(s) 105, 114, 537, 674, 1069
TaqI TCGA 2 cut(s) 36, 954
TasI AATT 2 cut(s) 87, 130
TatI WGTACW 3 cut(s) 532, 958, 1164
TfiI GAWTC 3 cut(s) 5, 140, 373
Tru1I TTAA 3 cut(s) 453, 516, 1201
Tru9I TTAA 3 cut(s) 453, 516, 1201
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 1 cut(s) 1180
TseI GCWGC 6 cut(s) 158, 263, 287, 320, 497, 1048
Tsp45I GTSAC 1 cut(s) 1180
TspDTI ATGAA 6 cut(s) 193, 447, 657, 675, 720, 788
TspGWI ACGGA 1 cut(s) 784
TspRI CASTG 1 cut(s) 189
Van91I CCANNNNNTGG 1 cut(s) 855
VpaK11BI GGWCC 1 cut(s) 125
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 2 cut(s) 87, 130
XspI CTAG 3 cut(s) 324, 509, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.