Rw5G027640

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000352
Physical Location & Seq
Forward (+)
41013907 .. 41014972
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027640.1

Sequence Viewer

Length: 876 bp
ATGGCCGACTCTAAATCTACTGTTAGCCTGAAGCTACTCATCGATTCAAAGCGTCAAAAGGTTCTGCTTGCCGAAGCGGGTAAGGACTTCGTGGATTTTCTCTTCTCCCTTCTCTCTTTGCCTGTTGGCACTGTCATTAGGCTTCTGTCAAAGGATGGCATGGTTGGTAGCTTTGGTAAACTGTACGAGAGTGTTGAAAATCTAAATTTTACATATATGCAACGCACACTAAACAAGGATATCCTGCTCAAACCCAAGGCACCTGTTGCTGGACCTAATATCCTCGGTTTGTTGGCCAACGTCGACAAACCAGCTTCTACAAATAAGGTGTTCTACATGTGTTCGAATAATAATAGTAGTAATAATAAGACGAGCTCTTGCTATGTCTTTTCAAAACATTCTAAAAAAAAAATTATCTTTTCAAAAAAGAAAATTAATAAGACGAGCTCTTGCTATGTCTCTGATTATGCCAGTGAGCATGAACAGTCTAACTGTTCCGGTAGTACTGCGATTACTGCCCACCTTTATGTGACTGAAGATCCTAACGAGCAATGTCCACAGTGCAATCACAGCATGTCGTCTCAGGCAACTTACGTTGGTCAACCAAAGACCAGGGTAGCAACTTCTGGCGCCGATGCGGCAGGATATGTCAAAGGCCTTGTTACTTACATGATAATGGATGATCTGGAGGTGAAGCCTATGTCCACTATTTCAAGCATAACTCTGCTCAACAGGTTCAATATTAAGGATATTGGCTCCCTCGAAGAGAAGGTTGTTGATCTTGGCATGGATGAGGGTATGAAACTGCTGAAAGAATCCCTGCAATCCAAAGCGGTTCTGACAAATGTGTTCCTGGGGAAGAAAGCTGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

291

Amino Acids

31.77

Weight (kDa)

9.11

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 140 6.3e-30 Protein of unknown function (DUF674)
DUF674 PF05056 165 - 288 6.2e-19 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 259, 629
AccI GTMKAC 1 cut(s) 303
AciI CCGC 3 cut(s) 77, 638, 833
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 2 cut(s) 3, 294
AcsI RAATTY 1 cut(s) 205
AcuI CTGAAG 2 cut(s) 50, 555
AcyI GRCGYC 1 cut(s) 630
AfaI GTAC 2 cut(s) 185, 505
AfiI CCNNNNNNNGG 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 6 cut(s) 48, 197, 393, 423, 714, 739
AjnI CCWGG 2 cut(s) 611, 852
AluBI AGCT 6 cut(s) 34, 171, 314, 375, 447, 866
AluI AGCT 6 cut(s) 34, 171, 314, 375, 447, 866
Alw21I GWGCWC 2 cut(s) 377, 449
Alw26I GTCTC 2 cut(s) 463, 585
AlwI GGATC 1 cut(s) 533
AoxI GGCC 3 cut(s) 3, 294, 655
ApeKI GCWGC 2 cut(s) 866, 869
ApoI RAATTY 1 cut(s) 205
AseI ATTAAT 1 cut(s) 435
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 703
AsuII TTCGAA 1 cut(s) 344
AvaII GGWCC 1 cut(s) 272
BalI TGGCCA 1 cut(s) 296
BanI GGYRCC 2 cut(s) 259, 629
BanII GRGCYC 2 cut(s) 377, 449
Bbv12I GWGCWC 2 cut(s) 377, 449
BbvI GCAGC 2 cut(s) 853, 856
BccI CCATC 1 cut(s) 149
BciT130I CCWGG 2 cut(s) 613, 854
BcoDI GTCTC 2 cut(s) 463, 585
BfoI RGCGCY 1 cut(s) 633
BglI GCCNNNNNGGC 1 cut(s) 638
BisI GCNGC 3 cut(s) 639, 867, 870
BlsI GCNGC 3 cut(s) 640, 868, 871
BmcAI AGTACT 1 cut(s) 505
Bme1390I CCNGG 2 cut(s) 613, 854
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 3 cut(s) 261, 631, 757
BmrFI CCNGG 2 cut(s) 613, 854
BmsI GCATC 1 cut(s) 625
BpmI CTGGAG 1 cut(s) 707
Bpu14I TTCGAA 1 cut(s) 344
Bsa29I ATCGAT 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 4 cut(s) 255, 283, 612, 853
BsaWI WCCGGW 1 cut(s) 497
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 557
BseBI CCWGG 2 cut(s) 613, 854
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 4 cut(s) 255, 283, 612, 853
BseGI GGATG 3 cut(s) 160, 685, 796
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 1 cut(s) 596
BseNI ACTGG 1 cut(s) 471
BseXI GCAGC 2 cut(s) 853, 856
BshFI GGCC 3 cut(s) 5, 296, 657
BshNI GGYRCC 2 cut(s) 259, 629
BshVI ATCGAT 1 cut(s) 42
BsiHKAI GWGCWC 2 cut(s) 377, 449
BsiSI CCGG 1 cut(s) 498
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 2 cut(s) 463, 585
BsmBI CGTCTC 1 cut(s) 585
BsnI GGCC 3 cut(s) 5, 296, 657
Bsp119I TTCGAA 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 377, 449
Bsp143I GATC 3 cut(s) 538, 682, 778
BspACI CCGC 3 cut(s) 77, 638, 833
BspANI GGCC 3 cut(s) 5, 296, 657
BspCNI CTCAG 1 cut(s) 595
BspDI ATCGAT 1 cut(s) 42
BspLI GGNNCC 3 cut(s) 261, 631, 757
BspPI GGATC 1 cut(s) 533
BspT104I TTCGAA 1 cut(s) 344
BspT107I GGYRCC 2 cut(s) 259, 629
BsrDI GCAATG 1 cut(s) 557
BsrI ACTGG 1 cut(s) 471
BssECI CCNNGG 4 cut(s) 255, 283, 612, 853
BssMI GATC 3 cut(s) 538, 682, 778
BssNI GRCGYC 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 613, 854
Bst4CI ACNGT 6 cut(s) 22, 133, 183, 486, 494, 561
Bst6I CTCTTC 2 cut(s) 107, 759
BstACI GRCGYC 1 cut(s) 630
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBI TTCGAA 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 582
BstF5I GGATG 3 cut(s) 160, 685, 796
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 3 cut(s) 541, 685, 781
BstMAI GTCTC 2 cut(s) 463, 585
BstMBI GATC 3 cut(s) 538, 682, 778
BstMWI GCNNNNNNNGC 4 cut(s) 266, 515, 570, 638
BstNI CCWGG 2 cut(s) 613, 854
BstNSI RCATGY 2 cut(s) 340, 577
BstSCI CCNGG 2 cut(s) 611, 852
BstV1I GCAGC 2 cut(s) 853, 856
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
Bsu15I ATCGAT 1 cut(s) 42
BsuRI GGCC 3 cut(s) 5, 296, 657
BsuTUI ATCGAT 1 cut(s) 42
BtsCI GGATG 3 cut(s) 160, 685, 796
BtsIMutI CAGTG 3 cut(s) 129, 478, 566
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 1 cut(s) 632
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 1 cut(s) 42
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 184, 504
CviAII CATG 6 cut(s) 160, 337, 479, 574, 670, 787
CviQI GTAC 2 cut(s) 184, 504
DdeI CTNAG 1 cut(s) 582
DinI GGCGCC 1 cut(s) 631
DpnI GATC 3 cut(s) 540, 684, 780
DpnII GATC 3 cut(s) 538, 682, 778
EaeI YGGCCR 2 cut(s) 3, 294
Eam1104I CTCTTC 2 cut(s) 107, 759
EarI CTCTTC 2 cut(s) 107, 759
Ecl136II GAGCTC 2 cut(s) 375, 447
Eco130I CCWWGG 1 cut(s) 255
Eco147I AGGCCT 1 cut(s) 657
Eco24I GRGCYC 2 cut(s) 377, 449
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 272
Eco53kI GAGCTC 2 cut(s) 375, 447
Eco57I CTGAAG 2 cut(s) 50, 555
EcoICRI GAGCTC 2 cut(s) 375, 447
EcoRII CCWGG 2 cut(s) 611, 852
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 2 cut(s) 377, 449
EgeI GGCGCC 1 cut(s) 631
EheI GGCGCC 1 cut(s) 631
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 585
FaeI CATG 6 cut(s) 163, 340, 482, 577, 673, 790
FatI CATG 6 cut(s) 159, 336, 478, 573, 669, 786
FauI CCCGC 1 cut(s) 70
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 639, 867, 870
FokI GGATG 3 cut(s) 167, 692, 803
FriOI GRGCYC 2 cut(s) 377, 449
Fsp4HI GCNGC 3 cut(s) 639, 867, 870
GlaI GCGC 1 cut(s) 631
GluI GCNGC 3 cut(s) 639, 867, 870
GsuI CTGGAG 1 cut(s) 707
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 3 cut(s) 5, 296, 657
HapII CCGG 1 cut(s) 498
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 632
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 6 cut(s) 163, 340, 482, 577, 673, 790
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 2 cut(s) 304, 602
HindII GTYRAC 2 cut(s) 304, 602
HinfI GANTC 3 cut(s) 8, 44, 815
HpaII CCGG 1 cut(s) 498
HphI GGTGA 1 cut(s) 703
Hpy166II GTNNAC 5 cut(s) 179, 304, 557, 602, 705
Hpy188I TCNGA 2 cut(s) 463, 840
Hpy188III TCNNGA 1 cut(s) 686
Hpy8I GTNNAC 5 cut(s) 179, 304, 557, 602, 705
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 119, 763
HpyCH4III ACNGT 6 cut(s) 22, 133, 183, 486, 494, 561
HpyCH4IV ACGT 2 cut(s) 300, 594
HpyCH4V TGCA 4 cut(s) 220, 564, 823, 872
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 515, 570, 638
HpyF3I CTNAG 1 cut(s) 582
HpySE526I ACGT 2 cut(s) 300, 594
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 6 cut(s) 163, 340, 482, 577, 673, 790
HspAI GCGC 1 cut(s) 630
KasI GGCGCC 1 cut(s) 629
Kzo9I GATC 3 cut(s) 538, 682, 778
LmnI GCTCC 1 cut(s) 761
Lsp1109I GCAGC 2 cut(s) 853, 856
LweI GCATC 1 cut(s) 625
MaeII ACGT 2 cut(s) 300, 594
MaeIII GTNAC 2 cut(s) 529, 661
MalI GATC 3 cut(s) 540, 684, 780
MboI GATC 3 cut(s) 538, 682, 778
MboII GAAGA 4 cut(s) 94, 548, 776, 871
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 2 cut(s) 377, 449
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 3 cut(s) 205, 411, 432
MluNI TGGCCA 1 cut(s) 296
Mly113I GGCGCC 1 cut(s) 630
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 4 cut(s) 293, 682, 770, 787
Mox20I TGGCCA 1 cut(s) 296
MscI TGGCCA 1 cut(s) 296
MseI TTAA 2 cut(s) 435, 744
MslI CAYNNNNRTG 2 cut(s) 525, 674
Msp20I TGGCCA 1 cut(s) 296
MspI CCGG 1 cut(s) 498
MspR9I CCNGG 2 cut(s) 613, 854
MvaI CCWGG 2 cut(s) 613, 854
MwoI GCNNNNNNNGC 4 cut(s) 266, 515, 570, 638
NarI GGCGCC 1 cut(s) 630
NdeII GATC 3 cut(s) 538, 682, 778
NlaIII CATG 6 cut(s) 163, 340, 482, 577, 673, 790
NlaIV GGNNCC 3 cut(s) 261, 631, 757
NmuCI GTSAC 1 cut(s) 529
NspI RCATGY 2 cut(s) 340, 577
NspV TTCGAA 1 cut(s) 344
PceI AGGCCT 1 cut(s) 657
PciI ACATGT 1 cut(s) 336
PfeI GAWTC 2 cut(s) 44, 815
PkrI GCNGC 3 cut(s) 640, 868, 871
PleI GAGTC 1 cut(s) 2
PluTI GGCGCC 1 cut(s) 633
PpsI GAGTC 1 cut(s) 2
PscI ACATGT 1 cut(s) 336
PshBI ATTAAT 1 cut(s) 435
Psp124BI GAGCTC 2 cut(s) 377, 449
Psp6I CCWGG 2 cut(s) 611, 852
PspGI CCWGG 2 cut(s) 611, 852
PspN4I GGNNCC 3 cut(s) 261, 631, 757
PspPI GGNCC 1 cut(s) 272
PsuI RGATCY 1 cut(s) 538
RsaI GTAC 2 cut(s) 185, 505
RsaNI GTAC 2 cut(s) 184, 504
RseI CAYNNNNRTG 2 cut(s) 525, 674
SacI GAGCTC 2 cut(s) 377, 449
SalI GTCGAC 1 cut(s) 302
SaqAI TTAA 2 cut(s) 435, 744
SatI GCNGC 3 cut(s) 639, 867, 870
Sau3AI GATC 3 cut(s) 538, 682, 778
Sau96I GGNCC 1 cut(s) 272
ScaI AGTACT 1 cut(s) 505
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 2 cut(s) 613, 854
SduI GDGCHC 2 cut(s) 377, 449
SfaNI GCATC 1 cut(s) 625
SfoI GGCGCC 1 cut(s) 631
SfuI TTCGAA 1 cut(s) 344
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 2 cut(s) 525, 674
Sse9I AATT 3 cut(s) 205, 411, 432
SseBI AGGCCT 1 cut(s) 657
SsiI CCGC 3 cut(s) 77, 638, 833
SspDI GGCGCC 1 cut(s) 629
SspI AATATT 1 cut(s) 742
SstI GAGCTC 2 cut(s) 377, 449
StuI AGGCCT 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 611, 852
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 6 cut(s) 22, 133, 183, 486, 494, 561
TaiI ACGT 2 cut(s) 303, 597
TaqI TCGA 4 cut(s) 42, 303, 344, 762
TasI AATT 3 cut(s) 205, 411, 432
TatI WGTACW 1 cut(s) 503
TauI GCSGC 1 cut(s) 641
TfiI GAWTC 2 cut(s) 44, 815
Tru1I TTAA 2 cut(s) 435, 744
Tru9I TTAA 2 cut(s) 435, 744
TscAI CASTG 3 cut(s) 136, 478, 566
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 2 cut(s) 866, 869
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 2 cut(s) 495, 815
TspRI CASTG 3 cut(s) 136, 478, 566
VpaK11BI GGWCC 1 cut(s) 272
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 1 cut(s) 205
XceI RCATGY 2 cut(s) 340, 577
XmiI GTMKAC 1 cut(s) 303
ZrmI AGTACT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.