Rh1AG332300

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000352
Physical Location & Seq
Forward (+)
56768389 .. 56769542
1154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG332300.1

Sequence Viewer

Length: 735 bp
ATGGAAACCACCAGCACCCCCACCACGGATGTTACTTTGAAGCTCTTGATTGATACCAAGGGCCACAAAGTTCTGTATGCTGAATCGGGCAAGGAATTTGTGGATTTCCTTTTCACCATTATGTCGTTACCCGTTGGCACTGTCATTAGGCTCCTTAGCAAGGATAGCATGGTTGGTAGCTTAGGCAAACTTTATCATAGTATTGAAAGCATTGGTGACATTTACATGCTACCAGATTGTAACAGAGATACCTTGCTCAAACCCACAGCGGTAATAGGCTGCGGGGATGCCACTCTTTTGTTAACTAATGATGAGTCAGTGGAGAACAGAAAGGCTTACATGTGTGTGAACAGCCACCGGTATGTGGCTGATGACCCTAGAGCCATATGTCCAAAGTGCAAAAACTATATCTCCACCGAAGTGCCTTATGTTGCTCCGCAGGCTACTACTGCTGGATCTTCCGGGGGTGGTTGTGGATATGTGAAAGATGTTGTGACATACATGATTACGGATGATTTGGAGGTGAATCCCCTGTCTACCATAGCTTCTCTAACTATGCTTAACAAGTTCAATGTTAAGGAGGTTAGTTCCCTTGAAGAGAAGGTGGTTCATCTAGGCATGGATGAGGGTTTGAAACTGTTGAAGGCATCATTGGAGTCAAATTCTGTTCTCACCAATGTTTTCCTTGGGAAGAAAGAAGCATCTAACATTTCAATGCCCTGCAAGCGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

244

Amino Acids

26.61

Weight (kDa)

5.63

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 10 - 237 7.5e-43 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 536
AciI CCGC 3 cut(s) 269, 282, 437
AclWI GGATC 1 cut(s) 463
AcsI RAATTY 2 cut(s) 95, 661
AfiI CCNNNNNNNGG 3 cut(s) 25, 160, 364
AflIII ACRYGT 1 cut(s) 339
AgeI ACCGGT 1 cut(s) 357
AgsI TTSAA 7 cut(s) 40, 206, 571, 596, 634, 643, 714
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 3 cut(s) 43, 180, 545
AluI AGCT 3 cut(s) 43, 180, 545
AlwI GGATC 1 cut(s) 463
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 1 cut(s) 279
ApoI RAATTY 2 cut(s) 95, 661
AsiGI ACCGGT 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 61
AsuC2I CCSGG 1 cut(s) 463
AsuHPI GGTGA 4 cut(s) 106, 227, 535, 664
BbvI GCAGC 1 cut(s) 266
BcnI CCSGG 1 cut(s) 463
BfaI CTAG 2 cut(s) 378, 614
BisI GCNGC 1 cut(s) 280
BlsI GCNGC 1 cut(s) 281
Bme1390I CCNGG 1 cut(s) 463
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 152
BmrFI CCNGG 1 cut(s) 463
BmsI GCATC 3 cut(s) 277, 656, 710
Bpu10I CCTNAGC 2 cut(s) 155, 181
BpuMI CCSGG 1 cut(s) 463
BsaJI CCNNGG 4 cut(s) 24, 57, 462, 685
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 160, 364
Bse118I RCCGGY 1 cut(s) 357
BseDI CCNNGG 4 cut(s) 24, 57, 462, 685
BseGI GGATG 4 cut(s) 34, 292, 517, 628
BseLI CCNNNNNNNGG 3 cut(s) 25, 160, 364
BseXI GCAGC 1 cut(s) 266
BshFI GGCC 1 cut(s) 63
BshTI ACCGGT 1 cut(s) 357
BsiSI CCGG 2 cut(s) 358, 462
BslI CCNNNNNNNGG 3 cut(s) 25, 160, 364
BsnI GGCC 1 cut(s) 63
Bsp143I GATC 1 cut(s) 455
BspACI CCGC 3 cut(s) 269, 282, 437
BspANI GGCC 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 152
BspPI GGATC 1 cut(s) 463
BsrFI RCCGGY 1 cut(s) 357
BssAI RCCGGY 1 cut(s) 357
BssECI CCNNGG 4 cut(s) 24, 57, 462, 685
BssMI GATC 1 cut(s) 455
BssT1I CCWWGG 2 cut(s) 57, 685
Bst4CI ACNGT 2 cut(s) 142, 639
Bst6I CTCTTC 1 cut(s) 591
BstC8I GCNNGC 2 cut(s) 441, 725
BstDEI CTNAG 2 cut(s) 155, 181
BstDSI CCRYGG 1 cut(s) 24
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 4 cut(s) 34, 292, 517, 628
BstKTI GATC 1 cut(s) 458
BstMBI GATC 1 cut(s) 455
BstMWI GCNNNNNNNGC 4 cut(s) 165, 440, 449, 724
BstNSI RCATGY 2 cut(s) 229, 343
BstSCI CCNGG 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BsuRI GGCC 1 cut(s) 63
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 4 cut(s) 34, 292, 517, 628
BtsIMutI CAGTG 2 cut(s) 138, 324
Cac8I GCNNGC 2 cut(s) 441, 725
Cfr10I RCCGGY 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 61
CspAI ACCGGT 1 cut(s) 357
CviAII CATG 5 cut(s) 169, 226, 340, 502, 619
DdeI CTNAG 2 cut(s) 155, 181
DpnI GATC 1 cut(s) 457
DpnII GATC 1 cut(s) 455
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Eco130I CCWWGG 2 cut(s) 57, 685
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoT14I CCWWGG 2 cut(s) 57, 685
ErhI CCWWGG 2 cut(s) 57, 685
FaeI CATG 5 cut(s) 172, 229, 343, 505, 622
FatI CATG 5 cut(s) 168, 225, 339, 501, 618
FauI CCCGC 1 cut(s) 275
FauNDI CATATG 1 cut(s) 386
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 1 cut(s) 280
FokI GGATG 4 cut(s) 41, 299, 524, 635
Fsp4HI GCNGC 1 cut(s) 280
FspBI CTAG 2 cut(s) 378, 614
GluI GCNGC 1 cut(s) 280
HaeIII GGCC 1 cut(s) 63
HapII CCGG 2 cut(s) 358, 462
Hin1II CATG 5 cut(s) 172, 229, 343, 505, 622
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HinfI GANTC 4 cut(s) 83, 314, 526, 656
HpaI GTTAAC 1 cut(s) 303
HpaII CCGG 2 cut(s) 358, 462
HphI GGTGA 4 cut(s) 106, 227, 535, 664
Hpy166II GTNNAC 3 cut(s) 303, 349, 537
Hpy188III TCNNGA 1 cut(s) 46
Hpy8I GTNNAC 3 cut(s) 303, 349, 537
HpyAV CCTTC 2 cut(s) 595, 637
HpyCH4III ACNGT 2 cut(s) 142, 639
HpyCH4V TGCA 2 cut(s) 399, 723
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 440, 449, 724
HpyF3I CTNAG 2 cut(s) 155, 181
Hsp92II CATG 5 cut(s) 172, 229, 343, 505, 622
KspAI GTTAAC 1 cut(s) 303
Kzo9I GATC 1 cut(s) 455
LmnI GCTCC 2 cut(s) 156, 439
LpnPI CCDG 7 cut(s) 25, 246, 371, 425, 438, 475, 545
Lsp1109I GCAGC 1 cut(s) 266
LweI GCATC 3 cut(s) 277, 656, 710
MaeI CTAG 2 cut(s) 378, 614
MaeIII GTNAC 5 cut(s) 31, 126, 215, 239, 493
MalI GATC 1 cut(s) 457
MboI GATC 1 cut(s) 455
MboII GAAGA 3 cut(s) 450, 608, 703
MflI RGATCY 1 cut(s) 455
MluCI AATT 2 cut(s) 95, 661
MlyI GAGTC 2 cut(s) 323, 665
MnlI CCTC 3 cut(s) 514, 574, 619
MseI TTAA 3 cut(s) 302, 561, 576
MslI CAYNNNNRTG 6 cut(s) 119, 224, 344, 360, 419, 713
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 2 cut(s) 358, 462
MspR9I CCNGG 1 cut(s) 463
MwoI GCNNNNNNNGC 4 cut(s) 165, 440, 449, 724
NciI CCSGG 1 cut(s) 463
NdeI CATATG 1 cut(s) 386
NdeII GATC 1 cut(s) 455
NlaIII CATG 5 cut(s) 172, 229, 343, 505, 622
NlaIV GGNNCC 1 cut(s) 152
NmuCI GTSAC 2 cut(s) 215, 493
NspI RCATGY 2 cut(s) 229, 343
OliI CACNNNNGTG 1 cut(s) 419
PciI ACATGT 1 cut(s) 339
PfeI GAWTC 2 cut(s) 83, 526
PinAI ACCGGT 1 cut(s) 357
PkrI GCNGC 1 cut(s) 281
PleI GAGTC 2 cut(s) 322, 664
PpsI GAGTC 2 cut(s) 322, 664
PscI ACATGT 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 152
PspPI GGNCC 1 cut(s) 61
PsuI RGATCY 1 cut(s) 455
RseI CAYNNNNRTG 6 cut(s) 119, 224, 344, 360, 419, 713
SaqAI TTAA 3 cut(s) 302, 561, 576
SatI GCNGC 1 cut(s) 280
Sau3AI GATC 1 cut(s) 455
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 2 cut(s) 323, 665
ScrFI CCNGG 1 cut(s) 463
SetI ASST 7 cut(s) 45, 182, 254, 525, 547, 585, 606
SfaNI GCATC 3 cut(s) 277, 656, 710
SmiMI CAYNNNNRTG 6 cut(s) 119, 224, 344, 360, 419, 713
Sse9I AATT 2 cut(s) 95, 661
SsiI CCGC 3 cut(s) 269, 282, 437
SspMI CTAG 2 cut(s) 378, 614
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 2 cut(s) 57, 685
TaaI ACNGT 2 cut(s) 142, 639
TasI AATT 2 cut(s) 95, 661
TfiI GAWTC 2 cut(s) 83, 526
Tru1I TTAA 3 cut(s) 302, 561, 576
Tru9I TTAA 3 cut(s) 302, 561, 576
TscAI CASTG 2 cut(s) 145, 324
TseFI GTSAC 2 cut(s) 215, 493
TseI GCWGC 1 cut(s) 279
Tsp45I GTSAC 2 cut(s) 215, 493
TspDTI ATGAA 1 cut(s) 599
TspGWI ACGGA 2 cut(s) 41, 524
TspRI CASTG 2 cut(s) 145, 324
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 2 cut(s) 95, 661
XceI RCATGY 2 cut(s) 229, 343
XmiI GTMKAC 1 cut(s) 536
XspI CTAG 2 cut(s) 378, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.