pycom07g18010

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000352
Physical Location & Seq
Forward (+)
20551907 .. 20553170
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18010.1

Sequence Viewer

Length: 687 bp
ATGGCAACCACCACTGTGAGCTTGAAGCTCTTGATTGATACAAAGGGCCACAGAGTTCTGTTTGCCGAAGCCGGCAAGGACTTTGTGGATTTCCTTTTCACCCTTCTGTCTCTGCCGGTTGGAACTGTCATTAGGCTCCTCTCTAAGGACAACATGGTTGGTAGCTTAGGCCGACTTTACGACAGTGTCGAAAATCTTAATGACACATACATGCTTCCCAATCTCAACAAGGACACTCTGCTGAAACCCACGTCCCTCATTGGCTGCGCTAATGTCCCACATTTGCTTACAAGTGATGGACCAACGGTAAAAAAGGTGTACATGTGTGGGAGCTACCACCGGTACGTGGCTGATGACCCTAGGGCCGTTTGTCCACAGTGCAGATCTGCTATATCTATCGAGGTGCCTTATGTTGCTGCTCCAGCTACCGCTGGAGGATCGTCTGGTGGCGGAGCAGGTTTTGTGAAAGATGTTGTGACATACATGATTATGGATAATTTGGAAGTGAAGCCGATGTCCACCATATCATGTATTGCTTTGCTCAACAAGTTCAACGTTAAGGAGGTTGGCGCGCTTGAAGAGAAGGTTGTTCATATGGGCATGGACGAGGGGCTGAAGCTGCTGAAGGCATCATTGGAATCAAACAATGTTCTCTCCAATGTCTTCCTTTGTAAGAAAGAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

229

Amino Acids

24.65

Weight (kDa)

6.43

Isoelectric Point (pI)

28.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 4 - 226 4.6e-43 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 446
AccB1I GGYRCC 1 cut(s) 403
AccII CGCG 1 cut(s) 572
AciI CCGC 2 cut(s) 429, 450
AclI AACGTT 1 cut(s) 555
AclWI GGATC 1 cut(s) 445
AcuI CTGAAG 2 cut(s) 635, 644
AfaI GTAC 2 cut(s) 320, 344
AfiI CCNNNNNNNGG 2 cut(s) 145, 346
AflIII ACRYGT 1 cut(s) 321
AgeI ACCGGT 1 cut(s) 339
AgsI TTSAA 3 cut(s) 25, 553, 578
AjiI CACGTC 1 cut(s) 252
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 6 cut(s) 21, 28, 165, 333, 425, 619
AluI AGCT 6 cut(s) 21, 28, 165, 333, 425, 619
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 445
AoxI GGCC 3 cut(s) 46, 169, 363
ApeKI GCWGC 3 cut(s) 264, 416, 619
AsiGI ACCGGT 1 cut(s) 339
AspA2I CCTAGG 1 cut(s) 359
AspLEI GCGC 3 cut(s) 269, 572, 574
AspS9I GGNCC 3 cut(s) 46, 299, 363
AsuHPI GGTGA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 299
AvrII CCTAGG 1 cut(s) 359
BanI GGYRCC 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 655
BbvI GCAGC 3 cut(s) 251, 403, 606
BccI CCATC 1 cut(s) 290
BceAI ACGGC 1 cut(s) 350
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 360
BfuAI ACCTGC 1 cut(s) 446
BglII AGATCT 1 cut(s) 383
BisI GCNGC 3 cut(s) 265, 417, 620
BlnI CCTAGG 1 cut(s) 359
BlsI GCNGC 3 cut(s) 266, 418, 621
Bme18I GGWCC 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 252
BmgT120I GGNCC 3 cut(s) 46, 299, 363
BmiI GGNNCC 2 cut(s) 137, 405
BmsI GCATC 1 cut(s) 638
BpiI GAAGAC 1 cut(s) 655
BpmI CTGGAG 2 cut(s) 405, 453
Bpu10I CCTNAGC 1 cut(s) 166
BsaAI YACGTR 1 cut(s) 346
BsaJI CCNNGG 1 cut(s) 359
BsaWI WCCGGW 1 cut(s) 339
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 346
Bse118I RCCGGY 3 cut(s) 71, 115, 339
BseDI CCNNGG 1 cut(s) 359
BseLI CCNNNNNNNGG 2 cut(s) 145, 346
BsePI GCGCGC 1 cut(s) 570
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 3 cut(s) 251, 403, 606
BsgI GTGCAG 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 572
BshFI GGCC 3 cut(s) 48, 171, 365
BshNI GGYRCC 1 cut(s) 403
BshTI ACCGGT 1 cut(s) 339
BsiSI CCGG 3 cut(s) 72, 116, 340
BslFI GGGAC 2 cut(s) 238, 260
BslI CCNNNNNNNGG 2 cut(s) 145, 346
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 2 cut(s) 238, 260
BsnI GGCC 3 cut(s) 48, 171, 365
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 2 cut(s) 383, 437
BspACI CCGC 2 cut(s) 429, 450
BspANI GGCC 3 cut(s) 48, 171, 365
BspFNI CGCG 1 cut(s) 572
BspLI GGNNCC 2 cut(s) 137, 405
BspMI ACCTGC 1 cut(s) 446
BspPI GGATC 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 403
BsrFI RCCGGY 3 cut(s) 71, 115, 339
BsrGI TGTACA 1 cut(s) 318
BssAI RCCGGY 3 cut(s) 71, 115, 339
BssECI CCNNGG 1 cut(s) 359
BssHII GCGCGC 1 cut(s) 570
BssMI GATC 2 cut(s) 383, 437
BssT1I CCWWGG 1 cut(s) 359
Bst4CI ACNGT 5 cut(s) 16, 127, 185, 307, 378
Bst6I CTCTTC 1 cut(s) 573
BstAUI TGTACA 1 cut(s) 318
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 73, 572
BstDEI CTNAG 2 cut(s) 144, 166
BstENI CCTNNNNNAGG 1 cut(s) 143
BstFNI CGCG 1 cut(s) 572
BstHHI GCGC 3 cut(s) 269, 572, 574
BstKTI GATC 2 cut(s) 386, 440
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 2 cut(s) 383, 437
BstMWI GCNNNNNNNGC 2 cut(s) 422, 619
BstNSI RCATGY 2 cut(s) 214, 325
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 3 cut(s) 251, 403, 606
BstV2I GAAGAC 1 cut(s) 655
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuRI GGCC 3 cut(s) 48, 171, 365
BtrI CACGTC 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 12, 190, 383
BveI ACCTGC 1 cut(s) 446
Cac8I GCNNGC 2 cut(s) 73, 572
CfoI GCGC 3 cut(s) 269, 572, 574
Cfr10I RCCGGY 3 cut(s) 71, 115, 339
Cfr13I GGNCC 3 cut(s) 46, 299, 363
Csp6I GTAC 2 cut(s) 319, 343
CspAI ACCGGT 1 cut(s) 339
CviAII CATG 6 cut(s) 154, 211, 322, 484, 528, 601
CviQI GTAC 2 cut(s) 319, 343
DdeI CTNAG 2 cut(s) 144, 166
DpnI GATC 2 cut(s) 385, 439
DpnII GATC 2 cut(s) 383, 437
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
EciI GGCGGA 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 635, 644
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 359
ErhI CCWWGG 1 cut(s) 359
FaeI CATG 6 cut(s) 157, 214, 325, 487, 531, 604
FaqI GGGAC 2 cut(s) 238, 260
FatI CATG 6 cut(s) 153, 210, 321, 483, 527, 600
FauNDI CATATG 1 cut(s) 594
Fnu4HI GCNGC 3 cut(s) 265, 417, 620
Fsp4HI GCNGC 3 cut(s) 265, 417, 620
FspBI CTAG 1 cut(s) 360
GlaI GCGC 3 cut(s) 268, 571, 573
GluI GCNGC 3 cut(s) 265, 417, 620
GsuI CTGGAG 2 cut(s) 405, 453
HaeIII GGCC 3 cut(s) 48, 171, 365
HapII CCGG 3 cut(s) 72, 116, 340
HhaI GCGC 3 cut(s) 269, 572, 574
Hin1II CATG 6 cut(s) 157, 214, 325, 487, 531, 604
Hin6I GCGC 3 cut(s) 267, 570, 572
HinP1I GCGC 3 cut(s) 267, 570, 572
HinfI GANTC 1 cut(s) 638
HpaII CCGG 3 cut(s) 72, 116, 340
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 3 cut(s) 319, 374, 519
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 3 cut(s) 319, 374, 519
HpyAV CCTTC 3 cut(s) 113, 577, 619
HpyCH4III ACNGT 5 cut(s) 16, 127, 185, 307, 378
HpyCH4IV ACGT 3 cut(s) 251, 345, 555
HpyCH4V TGCA 1 cut(s) 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 619
HpyF3I CTNAG 2 cut(s) 144, 166
HpySE526I ACGT 3 cut(s) 251, 345, 555
Hsp92II CATG 6 cut(s) 157, 214, 325, 487, 531, 604
HspAI GCGC 3 cut(s) 267, 570, 572
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Kzo9I GATC 2 cut(s) 383, 437
LmnI GCTCC 4 cut(s) 141, 330, 424, 452
LpnPI CCDG 7 cut(s) 85, 129, 353, 417, 429, 435, 441
Lsp1109I GCAGC 3 cut(s) 251, 403, 606
LweI GCATC 1 cut(s) 638
MaeI CTAG 1 cut(s) 360
MaeII ACGT 3 cut(s) 251, 345, 555
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 385, 439
MboI GATC 2 cut(s) 383, 437
MboII GAAGA 2 cut(s) 590, 655
MflI RGATCY 1 cut(s) 383
MluCI AATT 1 cut(s) 496
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 7 cut(s) 149, 266, 394, 428, 556, 601, 673
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 2 cut(s) 198, 558
MslI CAYNNNNRTG 3 cut(s) 14, 209, 488
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 3 cut(s) 72, 116, 340
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 2 cut(s) 422, 619
NaeI GCCGGC 1 cut(s) 73
NdeI CATATG 1 cut(s) 594
NdeII GATC 2 cut(s) 383, 437
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 6 cut(s) 157, 214, 325, 487, 531, 604
NlaIV GGNNCC 2 cut(s) 137, 405
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 2 cut(s) 214, 325
OliI CACNNNNGTG 1 cut(s) 14
PauI GCGCGC 1 cut(s) 570
PciI ACATGT 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 638
PflFI GACNNNGTC 1 cut(s) 185
PinAI ACCGGT 1 cut(s) 339
PkrI GCNGC 3 cut(s) 266, 418, 621
Ppu21I YACGTR 1 cut(s) 346
PscI ACATGT 1 cut(s) 321
Psp1406I AACGTT 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 137, 405
PspPI GGNCC 3 cut(s) 46, 299, 363
PsuI RGATCY 1 cut(s) 383
PsyI GACNNNGTC 1 cut(s) 185
PteI GCGCGC 1 cut(s) 570
RsaI GTAC 2 cut(s) 320, 344
RsaNI GTAC 2 cut(s) 319, 343
RseI CAYNNNNRTG 3 cut(s) 14, 209, 488
SaqAI TTAA 2 cut(s) 198, 558
SatI GCNGC 3 cut(s) 265, 417, 620
Sau3AI GATC 2 cut(s) 383, 437
Sau96I GGNCC 3 cut(s) 46, 299, 363
SfaNI GCATC 1 cut(s) 638
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 3 cut(s) 14, 209, 488
Sse9I AATT 1 cut(s) 496
SsiI CCGC 2 cut(s) 429, 450
SspMI CTAG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 359
TaaI ACNGT 5 cut(s) 16, 127, 185, 307, 378
TaiI ACGT 3 cut(s) 254, 348, 558
TaqI TCGA 2 cut(s) 189, 399
TasI AATT 1 cut(s) 496
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 2 cut(s) 198, 558
Tru9I TTAA 2 cut(s) 198, 558
TscAI CASTG 3 cut(s) 19, 190, 383
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 3 cut(s) 264, 416, 619
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 1 cut(s) 581
TspRI CASTG 3 cut(s) 19, 190, 383
Tth111I GACNNNGTC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 299
XagI CCTNNNNNAGG 1 cut(s) 143
XceI RCATGY 2 cut(s) 214, 325
XmaJI CCTAGG 1 cut(s) 359
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.