Rh5DG315800

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000352
Physical Location & Seq
Forward (+)
40500179 .. 40501141
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG315800.1

Sequence Viewer

Length: 777 bp
ATGGCCGACTCTAAATCTACAGTCAGCCTGAAGCTACTCATCGATACAAAGCGTCAAAAGGTTCTGTTTGCTGAAGCGGGTAAGGACTTTGTGGATTTTCTTTTCACCCTTCTCTCTTTGCCAGTTGGCACTGTTATTAGGCTTCTGTCAAAGGATGGCATGGTTGGTAGCTTTGGTAAACTGTATGACAGTGTTGAAAATCTAGATTTAACATATATGGAACCCAAACTAAACAAGGATATCCTGCTCAAACCCAAGGCACCTGTTGCTGGACCTAATATCCTCGGTTTGTTGGACTTGGCCAACGTTGACGTACCAGCTGCTAGAAATAAGGTGTTCTACATGTGTTCGAAAAGGAATAACAATAAAAATATTAATAGGCGTGGACAGTGTAACTGTTCCGGCTCTGCGCCTACCGCCCACCTTTATGTGACTGAAGATCCTAAAGAACAATGTCCAAAGTGCAATCACCATATGTCTTCTCAGGCGACTTATGTTGGTCAACCAAAGACCACGGTAGCAGCTTCTCGCTTCGGTGCAGCAGGATATGTCAAAGGCCTAGTTACGTACATTGTAATGGATGATCTGGAGGTGAAGCCTATGTCCACTATTTCAAGCATAACTCTGCTCAACAGGTTCAATGTTAAGAATGTTGGCACCCTCGAACAGAAGGTTGTAGATCTTGGCATGAATGAGGGTGTGAAACTGCTGAAAGAATCCCTACAATCCAAAGCAGTTCTCACAAATGTGTTCCTGGTGAAGAAAGCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

258

Amino Acids

28.17

Weight (kDa)

9.39

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 255 1.9e-47 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 259, 656
AciI CCGC 2 cut(s) 77, 417
AclI AACGTT 1 cut(s) 306
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 2 cut(s) 3, 300
AcuI CTGAAG 3 cut(s) 50, 93, 456
AfaI GTAC 2 cut(s) 315, 569
AfiI CCNNNNNNNGG 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 3 cut(s) 197, 615, 640
AjnI CCWGG 1 cut(s) 753
AleI CACNNNNGTG 1 cut(s) 746
AluBI AGCT 5 cut(s) 34, 171, 320, 524, 767
AluI AGCT 5 cut(s) 34, 171, 320, 524, 767
AlwI GGATC 1 cut(s) 434
AoxI GGCC 3 cut(s) 3, 300, 556
ApeKI GCWGC 5 cut(s) 320, 521, 539, 767, 770
AseI ATTAAT 1 cut(s) 375
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 4 cut(s) 97, 461, 604, 769
AsuII TTCGAA 1 cut(s) 350
AvaII GGWCC 1 cut(s) 272
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 2 cut(s) 259, 656
BbsI GAAGAC 1 cut(s) 471
BbvI GCAGC 5 cut(s) 307, 533, 551, 754, 757
BccI CCATC 1 cut(s) 149
BciT130I CCWGG 1 cut(s) 755
BfaI CTAG 3 cut(s) 203, 324, 560
BfmI CTRYAG 1 cut(s) 18
BglII AGATCT 1 cut(s) 679
BisI GCNGC 5 cut(s) 321, 522, 540, 768, 771
BlsI GCNGC 5 cut(s) 322, 523, 541, 769, 772
Bme1390I CCNGG 1 cut(s) 755
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 3 cut(s) 222, 261, 658
BmrFI CCNGG 1 cut(s) 755
BpiI GAAGAC 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 608
Bpu14I TTCGAA 1 cut(s) 350
Bsa29I ATCGAT 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 567
BsaJI CCNNGG 3 cut(s) 255, 283, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 755
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 3 cut(s) 255, 283, 513
BseGI GGATG 2 cut(s) 160, 586
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMII CTCAG 1 cut(s) 497
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 5 cut(s) 307, 533, 551, 754, 757
BsgI GTGCAG 1 cut(s) 558
BshFI GGCC 3 cut(s) 5, 302, 558
BshNI GGYRCC 2 cut(s) 259, 656
BshVI ATCGAT 1 cut(s) 42
BsiSI CCGG 1 cut(s) 402
BslI CCNNNNNNNGG 1 cut(s) 269
BsnI GGCC 3 cut(s) 5, 302, 558
Bsp119I TTCGAA 1 cut(s) 350
Bsp143I GATC 3 cut(s) 439, 583, 679
BspACI CCGC 2 cut(s) 77, 417
BspANI GGCC 3 cut(s) 5, 302, 558
BspCNI CTCAG 1 cut(s) 496
BspDI ATCGAT 1 cut(s) 42
BspLI GGNNCC 3 cut(s) 222, 261, 658
BspPI GGATC 1 cut(s) 434
BspT104I TTCGAA 1 cut(s) 350
BspT107I GGYRCC 2 cut(s) 259, 656
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 3 cut(s) 255, 283, 513
BssMI GATC 3 cut(s) 439, 583, 679
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 7 cut(s) 22, 133, 183, 191, 390, 398, 517
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 567
BstBI TTCGAA 1 cut(s) 350
BstDEI CTNAG 1 cut(s) 483
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 2 cut(s) 160, 586
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 3 cut(s) 442, 586, 682
BstMBI GATC 3 cut(s) 439, 583, 679
BstMWI GCNNNNNNNGC 2 cut(s) 266, 416
BstNI CCWGG 1 cut(s) 755
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 753
BstSFI CTRYAG 1 cut(s) 18
BstSNI TACGTA 1 cut(s) 567
BstV1I GCAGC 5 cut(s) 307, 533, 551, 754, 757
BstV2I GAAGAC 1 cut(s) 471
BstX2I RGATCY 2 cut(s) 439, 679
BstYI RGATCY 2 cut(s) 439, 679
Bsu15I ATCGAT 1 cut(s) 42
BsuRI GGCC 3 cut(s) 5, 302, 558
BsuTUI ATCGAT 1 cut(s) 42
BtgI CCRYGG 1 cut(s) 513
BtsCI GGATG 2 cut(s) 160, 586
BtsIMutI CAGTG 3 cut(s) 129, 196, 395
CfoI GCGC 1 cut(s) 412
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 1 cut(s) 42
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 314, 568
CviAII CATG 4 cut(s) 160, 343, 688, 774
CviQI GTAC 2 cut(s) 314, 568
DdeI CTNAG 1 cut(s) 483
DpnI GATC 3 cut(s) 441, 585, 681
DpnII GATC 3 cut(s) 439, 583, 679
EaeI YGGCCR 2 cut(s) 3, 300
Eco105I TACGTA 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 255
Eco147I AGGCCT 1 cut(s) 558
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 3 cut(s) 50, 93, 456
EcoRII CCWGG 1 cut(s) 753
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 4 cut(s) 163, 346, 691, 777
FatI CATG 4 cut(s) 159, 342, 687, 773
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 474
Fnu4HI GCNGC 5 cut(s) 321, 522, 540, 768, 771
FokI GGATG 2 cut(s) 167, 593
Fsp4HI GCNGC 5 cut(s) 321, 522, 540, 768, 771
FspBI CTAG 3 cut(s) 203, 324, 560
GlaI GCGC 1 cut(s) 411
GluI GCNGC 5 cut(s) 321, 522, 540, 768, 771
GsuI CTGGAG 1 cut(s) 608
HaeIII GGCC 3 cut(s) 5, 302, 558
HapII CCGG 1 cut(s) 402
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 412
Hin1II CATG 4 cut(s) 163, 346, 691, 777
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HincII GTYRAC 2 cut(s) 310, 503
HindII GTYRAC 2 cut(s) 310, 503
HinfI GANTC 2 cut(s) 8, 716
HpaII CCGG 1 cut(s) 402
HphI GGTGA 4 cut(s) 97, 461, 604, 769
Hpy166II GTNNAC 5 cut(s) 179, 310, 386, 503, 606
Hpy188III TCNNGA 2 cut(s) 203, 587
Hpy8I GTNNAC 5 cut(s) 179, 310, 386, 503, 606
HpyAV CCTTC 2 cut(s) 119, 664
HpyCH4III ACNGT 7 cut(s) 22, 133, 183, 191, 390, 398, 517
HpyCH4IV ACGT 3 cut(s) 306, 312, 566
HpyCH4V TGCA 3 cut(s) 465, 539, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 416
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 3 cut(s) 306, 312, 566
Hsp92II CATG 4 cut(s) 163, 346, 691, 777
HspAI GCGC 1 cut(s) 410
Kzo9I GATC 3 cut(s) 439, 583, 679
Lsp1109I GCAGC 5 cut(s) 307, 533, 551, 754, 757
MaeI CTAG 3 cut(s) 203, 324, 560
MaeII ACGT 3 cut(s) 306, 312, 566
MaeIII GTNAC 3 cut(s) 392, 430, 562
MalI GATC 3 cut(s) 441, 585, 681
MboI GATC 3 cut(s) 439, 583, 679
MboII GAAGA 3 cut(s) 449, 471, 772
MflI RGATCY 2 cut(s) 439, 679
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 4 cut(s) 293, 583, 671, 688
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 3 cut(s) 209, 375, 645
MslI CAYNNNNRTG 3 cut(s) 426, 575, 746
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 1 cut(s) 755
MvaI CCWGG 1 cut(s) 755
MwoI GCNNNNNNNGC 2 cut(s) 266, 416
NdeI CATATG 1 cut(s) 474
NdeII GATC 3 cut(s) 439, 583, 679
NlaIII CATG 4 cut(s) 163, 346, 691, 777
NlaIV GGNNCC 3 cut(s) 222, 261, 658
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 1 cut(s) 346
NspV TTCGAA 1 cut(s) 350
OliI CACNNNNGTG 1 cut(s) 746
PceI AGGCCT 1 cut(s) 558
PciI ACATGT 1 cut(s) 342
PfeI GAWTC 1 cut(s) 716
PkrI GCNGC 5 cut(s) 322, 523, 541, 769, 772
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Ppu21I YACGTR 1 cut(s) 567
PscI ACATGT 1 cut(s) 342
PshBI ATTAAT 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 753
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 3 cut(s) 222, 261, 658
PspPI GGNCC 1 cut(s) 272
PsuI RGATCY 2 cut(s) 439, 679
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 2 cut(s) 315, 569
RsaNI GTAC 2 cut(s) 314, 568
RseI CAYNNNNRTG 3 cut(s) 426, 575, 746
SaqAI TTAA 3 cut(s) 209, 375, 645
SatI GCNGC 5 cut(s) 321, 522, 540, 768, 771
Sau3AI GATC 3 cut(s) 439, 583, 679
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 755
SfcI CTRYAG 1 cut(s) 18
SfuI TTCGAA 1 cut(s) 350
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 3 cut(s) 426, 575, 746
SnaBI TACGTA 1 cut(s) 567
SseBI AGGCCT 1 cut(s) 558
SsiI CCGC 2 cut(s) 77, 417
SspI AATATT 1 cut(s) 373
SspMI CTAG 3 cut(s) 203, 324, 560
StuI AGGCCT 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 753
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 7 cut(s) 22, 133, 183, 191, 390, 398, 517
TaiI ACGT 3 cut(s) 309, 315, 569
TaqI TCGA 3 cut(s) 42, 350, 663
TfiI GAWTC 1 cut(s) 716
Tru1I TTAA 3 cut(s) 209, 375, 645
Tru9I TTAA 3 cut(s) 209, 375, 645
TscAI CASTG 3 cut(s) 136, 196, 395
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 5 cut(s) 320, 521, 539, 767, 770
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 1 cut(s) 704
TspRI CASTG 3 cut(s) 136, 196, 395
VpaK11BI GGWCC 1 cut(s) 272
VspI ATTAAT 1 cut(s) 375
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 1 cut(s) 346
XspI CTAG 3 cut(s) 203, 324, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.