FvH4_7g21330

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000352
Physical Location & Seq
Reverse (-)
17112035 .. 17113204
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21330.t1

Sequence Viewer

Length: 771 bp
ATGGCAACTATTAGCTTGAAGCTCCTCGTCGACACAGAGCAGAACAAGGTTCTGTTCGCCGAAGCAGGTAAGGACTTTGTGGACTTCCTATTCACCCTTCTTTCTTTTCCCGCCGGCACCATCATCAGGCTCCTCTCAAAGGATACCATGGTTGGAAGCCTGGGCAAACTTTACGAAAGCATTGAAAATTTCAGTGATACATACATCCAACCCAATCTCAACAAAGACATTCTCCTGAAACCCAAAGAACCAGGTTCTGTCCCAAATAACCTAGGGTTGCTGACCAATGTCGAAACAACAGAAAGAAAGGTGATCTACACTTGTTCGAATAGTTACAGCAGATACTATGACTACAGCTACCACACCCATAGCGACACGCACCCGTATTTTTCAGAGGATCCTAAAGCACAATGTCCAGAGTGTGGTGAAAGTCTGAGTTGTAAGATGAGTTATGTTCCTCCACCGAGTAGTAGTGGTAGTGCAGAATCTTCCGCCGGGAAAGGGGTAGGGTTTGTGAAGCCGGTTGTTACATACATGATAATGGATGACTTGGAAGTGAAGCCTATGTCTACCATTTCAACAATAGCTCTACTCAACCAGTTCAATGTTAGAGATTTTGGTGCCCTGGAACAGAAGGTTGTTGATCTGGGCATGGATGAGGGCGTGAAATTGCTCAAGGAATCATTGCAATCGAAGTCAGTCCTAACTAATGTGTTTCTTGGGGGAGTGTCTGGAAGAGTGAAGCATGAACATCTCAACTCTGCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

257

Amino Acids

28.15

Weight (kDa)

5.42

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 3 - 243 8.7e-49 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 56
AccB1I GGYRCC 2 cut(s) 116, 620
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 2 cut(s) 30, 569
AciI CCGC 2 cut(s) 111, 492
AclWI GGATC 2 cut(s) 392, 405
AcsI RAATTY 1 cut(s) 187
AfiI CCNNNNNNNGG 4 cut(s) 126, 139, 422, 501
AgsI TTSAA 4 cut(s) 19, 185, 579, 604
AjnI CCWGG 3 cut(s) 159, 250, 624
AluBI AGCT 4 cut(s) 15, 22, 357, 587
AluI AGCT 4 cut(s) 15, 22, 357, 587
AlwI GGATC 2 cut(s) 392, 405
AlwNI CAGNNNCTG 1 cut(s) 257
ApoI RAATTY 1 cut(s) 187
AspA2I CCTAGG 1 cut(s) 271
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 3 cut(s) 85, 322, 437
AsuII TTCGAA 1 cut(s) 326
AvrII CCTAGG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 625
BaeI ACNNNNGTAYC 2 cut(s) 135, 168
BamHI GGATCC 1 cut(s) 397
BanI GGYRCC 2 cut(s) 116, 620
BccI CCATC 1 cut(s) 128
BciT130I CCWGG 3 cut(s) 161, 252, 626
BciVI GTATCC 1 cut(s) 136
BcnI CCSGG 1 cut(s) 496
BfaI CTAG 1 cut(s) 272
BfmI CTRYAG 1 cut(s) 352
BfuAI ACCTGC 1 cut(s) 56
BfuI GTATCC 1 cut(s) 136
BlnI CCTAGG 1 cut(s) 271
Bme1390I CCNGG 4 cut(s) 161, 252, 496, 626
BmiI GGNNCC 4 cut(s) 118, 131, 399, 622
BmrFI CCNGG 4 cut(s) 161, 252, 496, 626
BoxI GACNNNNGTC 1 cut(s) 287
Bpu14I TTCGAA 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 659
BpuMI CCSGG 1 cut(s) 496
BsaJI CCNNGG 4 cut(s) 147, 160, 271, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 139, 422, 501
Bse118I RCCGGY 2 cut(s) 113, 520
Bse1I ACTGG 1 cut(s) 598
Bse3DI GCAATG 1 cut(s) 683
BseBI CCWGG 3 cut(s) 161, 252, 626
BseDI CCNNGG 4 cut(s) 147, 160, 271, 624
BseGI GGATG 3 cut(s) 204, 550, 661
BseLI CCNNNNNNNGG 4 cut(s) 126, 139, 422, 501
BseMI GCAATG 1 cut(s) 683
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 598
BseRI GAGGAG 2 cut(s) 14, 122
BseSI GKGCMC 1 cut(s) 625
BsgI GTGCAG 1 cut(s) 501
BshNI GGYRCC 2 cut(s) 116, 620
BsiSI CCGG 3 cut(s) 114, 495, 521
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 4 cut(s) 126, 139, 422, 501
BsmFI GGGAC 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 3 cut(s) 312, 397, 643
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 2 cut(s) 111, 492
BspCNI CTCAG 1 cut(s) 426
BspLI GGNNCC 4 cut(s) 118, 131, 399, 622
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 2 cut(s) 392, 405
BspT104I TTCGAA 1 cut(s) 326
BspT107I GGYRCC 2 cut(s) 116, 620
BsrDI GCAATG 1 cut(s) 683
BsrFI RCCGGY 2 cut(s) 113, 520
BsrI ACTGG 1 cut(s) 598
BssAI RCCGGY 2 cut(s) 113, 520
BssECI CCNNGG 4 cut(s) 147, 160, 271, 624
BssMI GATC 3 cut(s) 312, 397, 643
BssT1I CCWWGG 2 cut(s) 147, 271
Bst2UI CCWGG 3 cut(s) 161, 252, 626
Bst6I CTCTTC 1 cut(s) 730
BstBI TTCGAA 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 434
BstDSI CCRYGG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 3 cut(s) 204, 550, 661
BstKTI GATC 3 cut(s) 315, 400, 646
BstMBI GATC 3 cut(s) 312, 397, 643
BstNI CCWGG 3 cut(s) 161, 252, 626
BstPAI GACNNNNGTC 1 cut(s) 287
BstSCI CCNGG 4 cut(s) 159, 250, 494, 624
BstSFI CTRYAG 1 cut(s) 352
BstSLI GKGCMC 1 cut(s) 625
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuI GTATCC 1 cut(s) 136
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 3 cut(s) 204, 550, 661
BtsIMutI CAGTG 1 cut(s) 199
BveI ACCTGC 1 cut(s) 56
Cac8I GCNNGC 1 cut(s) 115
CaiI CAGNNNCTG 1 cut(s) 257
Cfr10I RCCGGY 2 cut(s) 113, 520
CsiI ACCWGGT 1 cut(s) 250
CviAII CATG 4 cut(s) 148, 535, 652, 746
CviJI RGCY 8 cut(s) 15, 22, 130, 159, 357, 520, 562, 587
CviKI_1 RGCY 8 cut(s) 15, 22, 130, 159, 357, 520, 562, 587
DdeI CTNAG 1 cut(s) 434
DpnI GATC 3 cut(s) 314, 399, 645
DpnII GATC 3 cut(s) 312, 397, 643
Eam1104I CTCTTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 730
EciI GGCGGA 1 cut(s) 481
Eco130I CCWWGG 2 cut(s) 147, 271
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoRII CCWGG 3 cut(s) 159, 250, 624
EcoT14I CCWWGG 2 cut(s) 147, 271
ErhI CCWWGG 2 cut(s) 147, 271
FaeI CATG 4 cut(s) 151, 538, 655, 749
FaqI GGGAC 1 cut(s) 245
FatI CATG 4 cut(s) 147, 534, 651, 745
FauI CCCGC 1 cut(s) 118
FblI GTMKAC 2 cut(s) 30, 569
FokI GGATG 3 cut(s) 191, 557, 668
FspBI CTAG 1 cut(s) 272
HapII CCGG 3 cut(s) 114, 495, 521
Hin1II CATG 4 cut(s) 151, 538, 655, 749
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 2 cut(s) 485, 680
HpaII CCGG 3 cut(s) 114, 495, 521
HphI GGTGA 3 cut(s) 85, 322, 437
Hpy166II GTNNAC 3 cut(s) 31, 82, 570
Hpy188I TCNGA 2 cut(s) 394, 435
Hpy188III TCNNGA 3 cut(s) 235, 416, 732
Hpy8I GTNNAC 3 cut(s) 31, 82, 570
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 107, 628
HpyCH4V TGCA 3 cut(s) 482, 688, 764
HpyF3I CTNAG 1 cut(s) 434
Hsp92II CATG 4 cut(s) 151, 538, 655, 749
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Kzo9I GATC 3 cut(s) 312, 397, 643
LmnI GCTCC 2 cut(s) 27, 135
MabI ACCWGGT 1 cut(s) 250
MaeI CTAG 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 332, 526
MalI GATC 3 cut(s) 314, 399, 645
MboI GATC 3 cut(s) 312, 397, 643
MboII GAAGA 2 cut(s) 480, 747
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 2 cut(s) 187, 668
MmeI TCCRAC 2 cut(s) 133, 232
MnlI CCTC 5 cut(s) 35, 143, 388, 468, 652
MroNI GCCGGC 1 cut(s) 113
MslI CAYNNNNRTG 1 cut(s) 539
MspI CCGG 3 cut(s) 114, 495, 521
MspR9I CCNGG 4 cut(s) 161, 252, 496, 626
MvaI CCWGG 3 cut(s) 161, 252, 626
NaeI GCCGGC 1 cut(s) 115
NciI CCSGG 1 cut(s) 496
NcoI CCATGG 1 cut(s) 147
NdeII GATC 3 cut(s) 312, 397, 643
NgoMIV GCCGGC 1 cut(s) 113
NlaIII CATG 4 cut(s) 151, 538, 655, 749
NlaIV GGNNCC 4 cut(s) 118, 131, 399, 622
NspV TTCGAA 1 cut(s) 326
PdiI GCCGGC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 485, 680
PflMI CCANNNNNTGG 1 cut(s) 422
PshAI GACNNNNGTC 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 159, 250, 624
PspGI CCWGG 3 cut(s) 159, 250, 624
PspN4I GGNNCC 4 cut(s) 118, 131, 399, 622
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 539
SalI GTCGAC 1 cut(s) 29
Sau3AI GATC 3 cut(s) 312, 397, 643
ScrFI CCNGG 4 cut(s) 161, 252, 496, 626
SduI GDGCHC 1 cut(s) 625
SexAI ACCWGGT 1 cut(s) 250
SfcI CTRYAG 1 cut(s) 352
SfuI TTCGAA 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 2 cut(s) 187, 668
SsiI CCGC 2 cut(s) 111, 492
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 4 cut(s) 159, 250, 494, 624
StyI CCWWGG 2 cut(s) 147, 271
TaqI TCGA 4 cut(s) 30, 291, 326, 692
TasI AATT 2 cut(s) 187, 668
TfiI GAWTC 2 cut(s) 485, 680
TscAI CASTG 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 762
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 422
XagI CCTNNNNNAGG 1 cut(s) 137
XapI RAATTY 1 cut(s) 187
XmaJI CCTAGG 1 cut(s) 271
XmiI GTMKAC 2 cut(s) 30, 569
XspI CTAG 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.