Rh6AG037200

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000352
Physical Location & Seq
Forward (+)
5058061 .. 5058655
595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG037200.1

Sequence Viewer

Length: 504 bp
ATGCTGAGATCCAAGGAACATGCTGCTGTATTTAACAAACTTCAGCTTGTGTTGAGAAACGAACCGGACCTTAAACCAATTATGCCGTCGAATGTGAAGAAGTTTTATATGTGTGAGAATTTCAAGTTGAAGCAACACGGCTCATGGGTGACAGATGACTCTAAAGCCATTTGTCCAATGTGCAGTACAACTATGTCGACCGGTGTCGGCTATGTTGCTCCTCCGGGAGAACCTACTTTGATGACCAGGGACGAAGGGTATGTGAGAGGTGTTGCTTCATACATGATAATGGACGATCTGAGAGTCAAACCTTTGTCTGTCTTTTCAAATTTGAATTTGCTTAAGAAGTGCATCAATGGCAGTAGTGGTTTTAATAACCTGGAAGAGAAGGTGGTTCATGTTGAAATGGATCAGGTAGAGAAATGGTTGAAGGCATTAATGGAGTCAAACTCAGTTCTTACCGATCTCTTCCTTGGGAAGAAAGTCAAGAACCCAATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

167

Amino Acids

18.79

Weight (kDa)

8.84

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 29 - 163 1.6e-17 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 197
AclWI GGATC 2 cut(s) 3, 417
AcsI RAATTY 3 cut(s) 118, 328, 334
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 1 cut(s) 187
AflII CTTAAG 1 cut(s) 341
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 6 cut(s) 124, 130, 327, 334, 404, 430
AjnI CCWGG 2 cut(s) 245, 378
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
AlwI GGATC 2 cut(s) 3, 417
ApeKI GCWGC 1 cut(s) 23
ApoI RAATTY 3 cut(s) 118, 328, 334
AseI ATTAAT 1 cut(s) 437
AsiGI ACCGGT 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 67
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 10
BceAI ACGGC 2 cut(s) 70, 154
BciT130I CCWGG 2 cut(s) 247, 380
BcnI CCSGG 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 341
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
Bme1390I CCNGG 3 cut(s) 225, 247, 380
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 67
BmrFI CCNGG 3 cut(s) 225, 247, 380
BmsI GCATC 1 cut(s) 360
BoxI GACNNNNGTC 1 cut(s) 203
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpuMI CCSGG 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 12, 246, 472
BsaWI WCCGGW 2 cut(s) 64, 200
Bse118I RCCGGY 1 cut(s) 200
BseBI CCWGG 2 cut(s) 247, 380
BseDI CCNNGG 3 cut(s) 12, 246, 472
BseMII CTCAG 2 cut(s) 290, 465
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 1 cut(s) 10
BsgI GTGCAG 1 cut(s) 202
Bsh1285I CGRYCG 1 cut(s) 201
BshTI ACCGGT 1 cut(s) 200
BsiEI CGRYCG 1 cut(s) 201
BsiSI CCGG 3 cut(s) 65, 201, 224
BslFI GGGAC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 263
Bsp143I GATC 4 cut(s) 8, 295, 409, 463
BspCNI CTCAG 2 cut(s) 291, 464
BspPI GGATC 2 cut(s) 3, 417
BspTI CTTAAG 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 12, 246, 472
BssMI GATC 4 cut(s) 8, 295, 409, 463
BssT1I CCWWGG 2 cut(s) 12, 472
Bst2UI CCWGG 2 cut(s) 247, 380
Bst6I CTCTTC 2 cut(s) 378, 473
BstAFI CTTAAG 1 cut(s) 341
BstDEI CTNAG 3 cut(s) 5, 299, 451
BstKTI GATC 4 cut(s) 11, 298, 412, 466
BstMBI GATC 4 cut(s) 8, 295, 409, 463
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstNI CCWGG 2 cut(s) 247, 380
BstNSI RCATGY 1 cut(s) 23
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 3 cut(s) 223, 245, 378
BstV1I GCAGC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 67
Csp6I GTAC 1 cut(s) 186
CspAI ACCGGT 1 cut(s) 200
CviAII CATG 4 cut(s) 20, 144, 283, 398
CviJI RGCY 4 cut(s) 46, 141, 167, 210
CviKI_1 RGCY 4 cut(s) 46, 141, 167, 210
CviQI GTAC 1 cut(s) 186
DdeI CTNAG 3 cut(s) 5, 299, 451
DpnI GATC 4 cut(s) 10, 297, 411, 465
DpnII GATC 4 cut(s) 8, 295, 409, 463
Eam1104I CTCTTC 2 cut(s) 378, 473
EarI CTCTTC 2 cut(s) 378, 473
Eco130I CCWWGG 2 cut(s) 12, 472
Eco47I GGWCC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 245, 378
EcoT14I CCWWGG 2 cut(s) 12, 472
ErhI CCWWGG 2 cut(s) 12, 472
FaeI CATG 4 cut(s) 23, 147, 286, 401
FaqI GGGAC 1 cut(s) 263
FatI CATG 4 cut(s) 19, 143, 282, 397
FblI GTMKAC 1 cut(s) 197
Fnu4HI GCNGC 1 cut(s) 24
Fsp4HI GCNGC 1 cut(s) 24
GluI GCNGC 1 cut(s) 24
HapII CCGG 3 cut(s) 65, 201, 224
Hin1II CATG 4 cut(s) 23, 147, 286, 401
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HinfI GANTC 3 cut(s) 158, 303, 443
HpaII CCGG 3 cut(s) 65, 201, 224
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 198
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 1 cut(s) 198
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 3 cut(s) 248, 382, 424
HpyCH4V TGCA 2 cut(s) 183, 351
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 3 cut(s) 5, 299, 451
Hsp92II CATG 4 cut(s) 23, 147, 286, 401
Kzo9I GATC 4 cut(s) 8, 295, 409, 463
LmnI GCTCC 1 cut(s) 223
LpnPI CCDG 8 cut(s) 78, 214, 232, 237, 259, 365, 392, 398
Lsp1109I GCAGC 1 cut(s) 10
LweI GCATC 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 148
MalI GATC 4 cut(s) 10, 297, 411, 465
MboI GATC 4 cut(s) 8, 295, 409, 463
MboII GAAGA 4 cut(s) 109, 395, 460, 490
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 8
MluCI AATT 5 cut(s) 78, 118, 328, 334, 495
MlyI GAGTC 3 cut(s) 152, 312, 452
MnlI CCTC 2 cut(s) 231, 260
MseI TTAA 5 cut(s) 33, 72, 342, 372, 437
MslI CAYNNNNRTG 1 cut(s) 287
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 3 cut(s) 65, 201, 224
MspR9I CCNGG 3 cut(s) 225, 247, 380
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 2 cut(s) 247, 380
MwoI GCNNNNNNNGC 1 cut(s) 357
NciI CCSGG 1 cut(s) 225
NdeII GATC 4 cut(s) 8, 295, 409, 463
NlaIII CATG 4 cut(s) 23, 147, 286, 401
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 1 cut(s) 23
PfoI TCCNGGA 1 cut(s) 223
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 1 cut(s) 25
PleI GAGTC 3 cut(s) 152, 311, 451
PpsI GAGTC 3 cut(s) 152, 311, 451
PshAI GACNNNNGTC 1 cut(s) 203
PshBI ATTAAT 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 245, 378
PspGI CCWGG 2 cut(s) 245, 378
PspPI GGNCC 1 cut(s) 67
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
RseI CAYNNNNRTG 1 cut(s) 287
SalI GTCGAC 1 cut(s) 196
SaqAI TTAA 5 cut(s) 33, 72, 342, 372, 437
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 4 cut(s) 8, 295, 409, 463
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 3 cut(s) 152, 312, 452
ScrFI CCNGG 3 cut(s) 225, 247, 380
SetI ASST 8 cut(s) 48, 72, 235, 271, 313, 381, 393, 417
SfaNI GCATC 1 cut(s) 360
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 5 cut(s) 78, 118, 328, 334, 495
StyD4I CCNGG 3 cut(s) 223, 245, 378
StyI CCWWGG 2 cut(s) 12, 472
TaqI TCGA 2 cut(s) 89, 197
TasI AATT 5 cut(s) 78, 118, 328, 334, 495
TatI WGTACW 1 cut(s) 185
Tru1I TTAA 5 cut(s) 33, 72, 342, 372, 437
Tru9I TTAA 5 cut(s) 33, 72, 342, 372, 437
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 267, 386
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 67
VspI ATTAAT 1 cut(s) 437
XapI RAATTY 3 cut(s) 118, 328, 334
XceI RCATGY 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.