Rorug01G0323500

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000352
Physical Location & Seq
Forward (+)
44234335 .. 44236325
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0323500.1

Sequence Viewer

Length: 516 bp
ATGGCCTCCACCTCCTCCACACTTACCATCTCCTCCTCTTCTACCCCTTTTGACACCAAGACTCCTCGCCACTCCGCCGCACCCTCACCTCAATGTGTGACACTCCCTACGCTTCCTCCACCGCCGGTGCAGTCTCAAAACCGCCCAGGGAAGACCACTGCCTATTGCCGTAAGATTGCTCGCAATGTCATGGCCATGGCCACTGGGGAAGCACCAGCAGAAGTTGCAACAACCGAGCTGCCAGAGGTTTTGAAGACAGTTCAAGAAGCTTGGGACAAAGTTGAAGACAAGTATGCAGTGTCTTCACTTGCAGTAGCTGGTGTTGTTGCAGTATGGGGCTCTACCGGGATGATTTCGGCAATTGATAGGCTTCCTTTGATTCCTGGTGCTCTCGAGCTTGTTGGCATTGGCTACACTGGGTGGTTTGCATACAAGAACCTGGTGTACAAACCAGACAGGGAAGCTTTGATACAGAAGATAAAAGACACATACAAAGACATAATTGGCAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

171

Amino Acids

18.25

Weight (kDa)

8.51

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAAD PF14159 85 - 169 2.5e-30 CAAD domains of cyanobacterial aminoacyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 75, 78, 122, 142
AcoI YGGCCR 2 cut(s) 192, 198
AdeI CACNNNGTG 1 cut(s) 420
AfaI GTAC 1 cut(s) 446
AgsI TTSAA 3 cut(s) 253, 263, 284
AjnI CCWGG 3 cut(s) 145, 382, 438
AluBI AGCT 6 cut(s) 238, 269, 317, 397, 464, 513
AluI AGCT 6 cut(s) 238, 269, 317, 397, 464, 513
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 1 cut(s) 138
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 392
AoxI GGCC 3 cut(s) 3, 192, 198
ApeKI GCWGC 3 cut(s) 238, 507, 510
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 392
BalI TGGCCA 2 cut(s) 194, 200
BanII GRGCYC 1 cut(s) 341
BarI GAAGNNNNNNTAC 2 cut(s) 453, 485
BbsI GAAGAC 4 cut(s) 158, 260, 291, 294
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 1 cut(s) 225
BccI CCATC 1 cut(s) 35
BceAI ACGGC 1 cut(s) 153
BciT130I CCWGG 3 cut(s) 147, 384, 440
BcnI CCSGG 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 1 cut(s) 514
BisI GCNGC 4 cut(s) 78, 239, 508, 511
BlsI GCNGC 4 cut(s) 79, 240, 509, 512
Bme1390I CCNGG 4 cut(s) 147, 346, 384, 440
BmeT110I CYCGRG 1 cut(s) 392
BmrFI CCNGG 4 cut(s) 147, 346, 384, 440
BmrI ACTGGG 2 cut(s) 213, 426
BmuI ACTGGG 2 cut(s) 213, 426
BpiI GAAGAC 4 cut(s) 158, 260, 291, 294
BpuMI CCSGG 1 cut(s) 346
BsaJI CCNNGG 3 cut(s) 145, 146, 195
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 100, 130
Bse118I RCCGGY 1 cut(s) 124
Bse1I ACTGG 2 cut(s) 208, 421
Bse3DI GCAATG 1 cut(s) 190
BseBI CCWGG 3 cut(s) 147, 384, 440
BseDI CCNNGG 3 cut(s) 145, 146, 195
BseGI GGATG 1 cut(s) 354
BseMI GCAATG 1 cut(s) 190
BseNI ACTGG 2 cut(s) 208, 421
BseRI GAGGAG 4 cut(s) 4, 22, 25, 54
BseXI GCAGC 1 cut(s) 225
BsgI GTGCAG 1 cut(s) 149
BshFI GGCC 3 cut(s) 5, 194, 200
BsiHKAI GWGCWC 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 2 cut(s) 125, 345
BslFI GGGAC 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 287
BsnI GGCC 3 cut(s) 5, 194, 200
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 341, 391
Bsp1407I TGTACA 1 cut(s) 444
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 4 cut(s) 75, 78, 122, 142
BspANI GGCC 3 cut(s) 5, 194, 200
BsrDI GCAATG 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 2 cut(s) 208, 421
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 145, 146, 195
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 3 cut(s) 147, 384, 440
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 43
BstAPI GCANNNNNTGC 1 cut(s) 224
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 181
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 354
BstMAI GTCTC 1 cut(s) 138
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 3 cut(s) 147, 384, 440
BstSCI CCNGG 4 cut(s) 145, 344, 382, 438
BstV1I GCAGC 1 cut(s) 225
BstV2I GAAGAC 4 cut(s) 158, 260, 291, 294
BsuRI GGCC 3 cut(s) 5, 194, 200
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 354
BtsI GCAGTG 2 cut(s) 156, 303
BtsIMutI CAGTG 4 cut(s) 156, 201, 303, 414
Cac8I GCNNGC 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 317
Cfr10I RCCGGY 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 438
Csp6I GTAC 1 cut(s) 445
CviAII CATG 2 cut(s) 190, 196
CviQI GTAC 1 cut(s) 445
DraIII CACNNNGTG 1 cut(s) 420
EaeI YGGCCR 2 cut(s) 192, 198
Eam1104I CTCTTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 43
EciI GGCGGA 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 392
EcoRII CCWGG 3 cut(s) 145, 382, 438
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 2 cut(s) 193, 199
FaiI YATR 7 cut(s) 191, 197, 294, 334, 430, 490, 500
FaqI GGGAC 1 cut(s) 287
FatI CATG 2 cut(s) 189, 195
Fnu4HI GCNGC 4 cut(s) 78, 239, 508, 511
FokI GGATG 1 cut(s) 361
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 4 cut(s) 78, 239, 508, 511
FspBI CTAG 1 cut(s) 514
GluI GCNGC 4 cut(s) 78, 239, 508, 511
HaeIII GGCC 3 cut(s) 5, 194, 200
HapII CCGG 2 cut(s) 125, 345
Hin1II CATG 2 cut(s) 193, 199
HindIII AAGCTT 2 cut(s) 267, 462
HinfI GANTC 2 cut(s) 61, 379
HpaII CCGG 2 cut(s) 125, 345
HphI GGTGA 1 cut(s) 78
Hpy166II GTNNAC 1 cut(s) 445
Hpy188III TCNNGA 2 cut(s) 263, 392
Hpy8I GTNNAC 1 cut(s) 445
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 6 cut(s) 130, 227, 296, 311, 329, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
Hsp92II CATG 2 cut(s) 193, 199
Lsp1109I GCAGC 1 cut(s) 225
MabI ACCWGGT 1 cut(s) 438
MaeI CTAG 1 cut(s) 514
MaeIII GTNAC 1 cut(s) 97
MboII GAAGA 6 cut(s) 30, 163, 265, 294, 296, 487
MfeI CAATTG 1 cut(s) 360
MhlI GDGCHC 2 cut(s) 341, 391
MlsI TGGCCA 2 cut(s) 194, 200
MluCI AATT 2 cut(s) 360, 501
MluNI TGGCCA 2 cut(s) 194, 200
MlyI GAGTC 1 cut(s) 55
Mox20I TGGCCA 2 cut(s) 194, 200
MscI TGGCCA 2 cut(s) 194, 200
MslI CAYNNNNRTG 2 cut(s) 91, 194
Msp20I TGGCCA 2 cut(s) 194, 200
MspI CCGG 2 cut(s) 125, 345
MspR9I CCNGG 4 cut(s) 147, 346, 384, 440
MunI CAATTG 1 cut(s) 360
MvaI CCWGG 3 cut(s) 147, 384, 440
MwoI GCNNNNNNNGC 1 cut(s) 224
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 195
NlaIII CATG 2 cut(s) 193, 199
NmuCI GTSAC 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 392
PasI CCCWGGG 1 cut(s) 146
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 4 cut(s) 79, 240, 509, 512
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
Psp6I CCWGG 3 cut(s) 145, 382, 438
PspGI CCWGG 3 cut(s) 145, 382, 438
PsrI GAACNNNNNNTAC 2 cut(s) 428, 460
PstNI CAGNNNCTG 1 cut(s) 317
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
RseI CAYNNNNRTG 2 cut(s) 91, 194
SatI GCNGC 4 cut(s) 78, 239, 508, 511
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 4 cut(s) 147, 346, 384, 440
SduI GDGCHC 2 cut(s) 341, 391
SexAI ACCWGGT 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 392
SgrAI CRCCGGYG 1 cut(s) 124
SlaI CTCGAG 1 cut(s) 392
SmiMI CAYNNNNRTG 2 cut(s) 91, 194
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 2 cut(s) 360, 501
SsiI CCGC 4 cut(s) 75, 78, 122, 142
SspMI CTAG 1 cut(s) 514
StyD4I CCNGG 4 cut(s) 145, 344, 382, 438
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 393
TasI AATT 2 cut(s) 360, 501
TatI WGTACW 1 cut(s) 444
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 379
TscAI CASTG 4 cut(s) 163, 208, 303, 421
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 3 cut(s) 238, 507, 510
Tsp45I GTSAC 1 cut(s) 97
TspRI CASTG 4 cut(s) 163, 208, 303, 421
XhoI CTCGAG 1 cut(s) 392
XspI CTAG 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.