Rh1AG335200

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000352
Physical Location & Seq
Forward (+)
56930545 .. 56933628
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG335200.1

Sequence Viewer

Length: 765 bp
ATGGCGACCTCTAATTCCGGTGCTGTTAGCCTGAAACTCCTGATTGATTCAAAGAATCAAAAGGTTCTCTTTGCTGAAGCCGGTAAGGATTTTGTGGATTTTCTTTTCACTCTTCTCTCTTTGCCTGTTGGTACTGTCATTAGGCTTCTCTCCAAGGGTGAGATGGTTGGTAGCTTTGGTAAACCGTATGAGAGGGTTGAAAACCTTGATGATACATATATGCAACCCGAACTAAACAAGGATATCCTGCTTCAACCCAGGGCACTAGTTACTGGACCTAATATCCTCAAGTTGGCCAACAACGTTGAATCAAGTGCTCCAAAGGTGCTCTACTTGTGTCCTGCATTTTATGACGGAACCCAGGCCCACCCGTACGTGACTGAAAATCCCAACGAGGAATGTCCATCGTGTACACGCACCATGGACCTCCAAACTACTTATGTCGGTCAGCCGACTACAACGGCAGGAACTTCTGGTGCCGTCGCTACTGGATATGTGAAAGGCGTTGTGACATACATGATAATGGATGATCTGGAGGTGAAGCCCATGTCCACCATTTCAAGCATCACTATGCTCAACAGGTTGAATATCAAGGATGTTGAGGCTCTTGAAGAGAAGGTTGTTGAACTTGCCATGGATAAGGGTGTGGAGCTGTTGAAGGAGTCTTTGTTGTCCAAATCGATCCTCACAAATGTCTTCCTTGGGAAGAAAACTGGGATTGGCATTGATATCTTGGAGCAACTACTACATAAAGGTGGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

254

Amino Acids

27.67

Weight (kDa)

5.09

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 237 1.5e-48 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 476
AclI AACGTT 1 cut(s) 303
AclWI GGATC 1 cut(s) 676
AcoI YGGCCR 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 3 cut(s) 133, 374, 412
AfiI CCNNNNNNNGG 1 cut(s) 292
AgsI TTSAA 9 cut(s) 51, 200, 254, 308, 561, 586, 611, 626, 658
AhlI ACTAGT 1 cut(s) 265
AjnI CCWGG 2 cut(s) 257, 360
AluBI AGCT 2 cut(s) 174, 652
AluI AGCT 2 cut(s) 174, 652
Alw21I GWGCWC 2 cut(s) 319, 330
AlwI GGATC 1 cut(s) 676
AoxI GGCC 2 cut(s) 294, 363
AspS9I GGNCC 3 cut(s) 275, 364, 424
AsuHPI GGTGA 2 cut(s) 170, 550
AvaII GGWCC 2 cut(s) 275, 424
BaeGI GKGCMC 1 cut(s) 265
BalI TGGCCA 1 cut(s) 296
BanI GGYRCC 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 688
Bbv12I GWGCWC 2 cut(s) 319, 330
BccI CCATC 2 cut(s) 157, 412
BceAI ACGGC 2 cut(s) 464, 477
BciT130I CCWGG 2 cut(s) 259, 362
BcuI ACTAGT 1 cut(s) 265
BfaI CTAG 1 cut(s) 266
Bme1390I CCNGG 2 cut(s) 259, 362
Bme18I GGWCC 2 cut(s) 275, 424
BmgT120I GGNCC 3 cut(s) 275, 364, 424
BmiI GGNNCC 2 cut(s) 358, 478
BmrFI CCNGG 2 cut(s) 259, 362
BmrI ACTGGG 1 cut(s) 723
BmsI GCATC 1 cut(s) 573
BmuI ACTGGG 1 cut(s) 723
BpiI GAAGAC 1 cut(s) 688
BpmI CTGGAG 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 272
Bsa29I ATCGAT 1 cut(s) 680
BsaAI YACGTR 1 cut(s) 376
BsaJI CCNNGG 7 cut(s) 153, 257, 258, 360, 420, 633, 700
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 3 cut(s) 277, 493, 718
BseBI CCWGG 2 cut(s) 259, 362
BseCI ATCGAT 1 cut(s) 680
BseDI CCNNGG 7 cut(s) 153, 257, 258, 360, 420, 633, 700
BseGI GGATG 2 cut(s) 532, 601
BseLI CCNNNNNNNGG 1 cut(s) 292
BseNI ACTGG 3 cut(s) 277, 493, 718
BseSI GKGCMC 1 cut(s) 265
BshFI GGCC 2 cut(s) 296, 365
BshNI GGYRCC 1 cut(s) 476
BshVI ATCGAT 1 cut(s) 680
BsiHKAI GWGCWC 2 cut(s) 319, 330
BsiSI CCGG 2 cut(s) 18, 81
BsiWI CGTACG 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 292
BsnI GGCC 2 cut(s) 296, 365
Bsp1286I GDGCHC 3 cut(s) 265, 319, 330
Bsp1407I TGTACA 1 cut(s) 410
Bsp143I GATC 2 cut(s) 529, 681
Bsp19I CCATGG 2 cut(s) 420, 633
BspANI GGCC 2 cut(s) 296, 365
BspDI ATCGAT 1 cut(s) 680
BspLI GGNNCC 2 cut(s) 358, 478
BspPI GGATC 1 cut(s) 676
BspT107I GGYRCC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 410
BsrI ACTGG 3 cut(s) 277, 493, 718
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 7 cut(s) 153, 257, 258, 360, 420, 633, 700
BssMI GATC 2 cut(s) 529, 681
BssT1I CCWWGG 4 cut(s) 153, 420, 633, 700
Bst2UI CCWGG 2 cut(s) 259, 362
Bst4CI ACNGT 2 cut(s) 136, 186
Bst6I CTCTTC 2 cut(s) 117, 606
BstAUI TGTACA 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 376
BstDSI CCRYGG 2 cut(s) 420, 633
BstF5I GGATG 2 cut(s) 532, 601
BstKTI GATC 2 cut(s) 532, 684
BstMBI GATC 2 cut(s) 529, 681
BstNI CCWGG 2 cut(s) 259, 362
BstSCI CCNGG 2 cut(s) 257, 360
BstSLI GKGCMC 1 cut(s) 265
BstV2I GAAGAC 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 680
BsuRI GGCC 2 cut(s) 296, 365
BsuTUI ATCGAT 1 cut(s) 680
BtgI CCRYGG 2 cut(s) 420, 633
BtsCI GGATG 2 cut(s) 532, 601
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 3 cut(s) 275, 364, 424
ClaI ATCGAT 1 cut(s) 680
Csp6I GTAC 3 cut(s) 132, 373, 411
CviAII CATG 4 cut(s) 421, 517, 547, 634
CviQI GTAC 3 cut(s) 132, 373, 411
DpnI GATC 2 cut(s) 531, 683
DpnII GATC 2 cut(s) 529, 681
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 117, 606
EarI CTCTTC 2 cut(s) 117, 606
Eco130I CCWWGG 4 cut(s) 153, 420, 633, 700
Eco32I GATATC 2 cut(s) 244, 730
Eco47I GGWCC 2 cut(s) 275, 424
Eco57I CTGAAG 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 257, 360
EcoRV GATATC 2 cut(s) 244, 730
EcoT14I CCWWGG 4 cut(s) 153, 420, 633, 700
ErhI CCWWGG 4 cut(s) 153, 420, 633, 700
FaeI CATG 4 cut(s) 424, 520, 550, 637
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 4 cut(s) 420, 516, 546, 633
FokI GGATG 2 cut(s) 539, 608
FspBI CTAG 1 cut(s) 266
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 2 cut(s) 296, 365
HapII CCGG 2 cut(s) 18, 81
Hin1II CATG 4 cut(s) 424, 520, 550, 637
HinfI GANTC 4 cut(s) 47, 55, 308, 662
HpaII CCGG 2 cut(s) 18, 81
HphI GGTGA 2 cut(s) 170, 550
Hpy166II GTNNAC 4 cut(s) 182, 411, 413, 552
Hpy188III TCNNGA 3 cut(s) 40, 533, 608
Hpy8I GTNNAC 4 cut(s) 182, 411, 413, 552
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 2 cut(s) 610, 652
HpyCH4III ACNGT 2 cut(s) 136, 186
HpyCH4IV ACGT 2 cut(s) 303, 375
HpyCH4V TGCA 2 cut(s) 223, 344
HpySE526I ACGT 2 cut(s) 303, 375
Hsp92II CATG 4 cut(s) 424, 520, 550, 637
Kzo9I GATC 2 cut(s) 529, 681
LmnI GCTCC 3 cut(s) 322, 649, 736
LweI GCATC 1 cut(s) 573
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 303, 375
MaeIII GTNAC 3 cut(s) 268, 376, 508
MalI GATC 2 cut(s) 531, 683
MboI GATC 2 cut(s) 529, 681
MboII GAAGA 4 cut(s) 104, 623, 688, 718
MhlI GDGCHC 3 cut(s) 265, 319, 330
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 1 cut(s) 13
MluNI TGGCCA 1 cut(s) 296
MlyI GAGTC 1 cut(s) 671
MnlI CCTC 8 cut(s) 19, 186, 296, 388, 437, 529, 595, 695
Mox20I TGGCCA 1 cut(s) 296
MscI TGGCCA 1 cut(s) 296
MseI TTAA 1 cut(s) 763
MslI CAYNNNNRTG 3 cut(s) 521, 569, 753
Msp20I TGGCCA 1 cut(s) 296
MspI CCGG 2 cut(s) 18, 81
MspR9I CCNGG 2 cut(s) 259, 362
MvaI CCWGG 2 cut(s) 259, 362
NcoI CCATGG 2 cut(s) 420, 633
NdeII GATC 2 cut(s) 529, 681
NlaIII CATG 4 cut(s) 424, 520, 550, 637
NlaIV GGNNCC 2 cut(s) 358, 478
NmuCI GTSAC 2 cut(s) 376, 508
PasI CCCWGGG 1 cut(s) 258
PfeI GAWTC 3 cut(s) 47, 55, 308
Pfl23II CGTACG 1 cut(s) 372
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 257, 360
PspGI CCWGG 2 cut(s) 257, 360
PspLI CGTACG 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 358, 478
PspPI GGNCC 3 cut(s) 275, 364, 424
RsaI GTAC 3 cut(s) 133, 374, 412
RsaNI GTAC 3 cut(s) 132, 373, 411
RseI CAYNNNNRTG 3 cut(s) 521, 569, 753
SaqAI TTAA 1 cut(s) 763
Sau3AI GATC 2 cut(s) 529, 681
Sau96I GGNCC 3 cut(s) 275, 364, 424
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 2 cut(s) 259, 362
SduI GDGCHC 3 cut(s) 265, 319, 330
SfaNI GCATC 1 cut(s) 573
SinI GGWCC 2 cut(s) 275, 424
SmiMI CAYNNNNRTG 3 cut(s) 521, 569, 753
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 265
Sse9I AATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 257, 360
StyI CCWWGG 4 cut(s) 153, 420, 633, 700
TaaI ACNGT 2 cut(s) 136, 186
TaiI ACGT 2 cut(s) 306, 378
TaqI TCGA 1 cut(s) 680
TaqII GACCGA 1 cut(s) 434
TasI AATT 1 cut(s) 13
TatI WGTACW 1 cut(s) 410
TfiI GAWTC 3 cut(s) 47, 55, 308
Tru1I TTAA 1 cut(s) 763
Tru9I TTAA 1 cut(s) 763
TseFI GTSAC 2 cut(s) 376, 508
Tsp45I GTSAC 2 cut(s) 376, 508
TspGWI ACGGA 1 cut(s) 369
VpaK11BI GGWCC 2 cut(s) 275, 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.