MD07G1190400.v1.1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000352
Physical Location & Seq
Forward (+)
27064598 .. 27065405
808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1190400.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGCTAACTCCGAGTCAACTGTTAGTTTGAAACTCCTAGTTGATTCAAAGCGTCACAAAGTTTTGTTTGCCGAAGCAGGCAAAGACTTTGTGGATTTTCTTTTCACTCTTCTATCGTTGCCTGTAGGGACTGTGATTAGGCTCCTCGCCAAGCATGGCATGGTTGGTAGCTTAGGGAAACTCTACGAGAGTGTTGAGACTCTGAGTGACACATACATGCAACCCAACCTTGACAAGGACACTCTGCTGAAACCCAAGCCAGCGATTGGAGGTACCGATGTCCTTCACTTATTGGCCGGCGATGAAGCAACTAGAAAGGTCTACATGTGTCGCAACTACCACCGCTATGTAGCTGATGATTCTCGAGCCATTTGTCCCTCGTGCAACTCCGCTATGTCAACAGAAGTGCCTCATGTAGCACCGCAGGTTGCTTCTGGAAGTTCCTCCGGCGAGGGTGGTGGTTATGTGAAAGGTCTTGTCACATACATGATAATGGATAACTTGGAAGTGAAGCCCATGTCTACCATTTCAGGCATTACAGTGCTGAACAAGTTCAATGTTAAGGACGTTGGGGCCCTTGAAGAGAAGGTGGTTCATCTTGGCATGCAAGAGGGTATAAGTCTGCTGAAGGCATCTCTCCAGTCAAACTATGTTCTCACCAGTGTTTTTCTGGGTAAGAAAGTGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

230

Amino Acids

24.7

Weight (kDa)

7.71

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 227 1.9e-48 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 415
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 266
AccI GTMKAC 2 cut(s) 321, 521
AciI CCGC 3 cut(s) 343, 390, 422
AcoI YGGCCR 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 647
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 2 cut(s) 235, 266
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 4 cut(s) 31, 48, 556, 581
AluBI AGCT 2 cut(s) 171, 353
AluI AGCT 2 cut(s) 171, 353
Alw26I GTCTC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 2 cut(s) 294, 573
ApaI GGGCCC 1 cut(s) 577
Asp700I GAANNNNTTC 1 cut(s) 551
Asp718I GGTACC 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 573, 574
AsuHPI GGTGA 1 cut(s) 649
AvaI CYCGRG 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 577
BaeI ACNNNNGTAYC 2 cut(s) 264, 297
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 577
BauI CACGAG 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 2 cut(s) 38, 312
BfmI CTRYAG 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 363
BmgT120I GGNCC 2 cut(s) 573, 574
BmiI GGNNCC 4 cut(s) 143, 274, 574, 575
BmsI GCATC 1 cut(s) 641
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 172
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 266
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 2 cut(s) 640, 660
BseLI CCNNNNNNNGG 2 cut(s) 235, 266
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 2 cut(s) 640, 660
BseRI GAGGAG 1 cut(s) 134
BseSI GKGCMC 1 cut(s) 577
BshFI GGCC 2 cut(s) 296, 575
BshNI GGYRCC 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 2 cut(s) 297, 447
BslFI GGGAC 2 cut(s) 142, 360
BslI CCNNNNNNNGG 2 cut(s) 235, 266
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 2 cut(s) 142, 360
BsnI GGCC 2 cut(s) 296, 575
BsoBI CYCGRG 1 cut(s) 363
Bsp120I GGGCCC 1 cut(s) 573
Bsp1286I GDGCHC 1 cut(s) 577
BspACI CCGC 3 cut(s) 343, 390, 422
BspANI GGCC 2 cut(s) 296, 575
BspCNI CTCAG 1 cut(s) 195
BspLI GGNNCC 4 cut(s) 143, 274, 574, 575
BspMI ACCTGC 1 cut(s) 415
BspT107I GGYRCC 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 2 cut(s) 640, 660
BssAI RCCGGY 1 cut(s) 296
BssSI CACGAG 1 cut(s) 379
Bst2BI CACGAG 1 cut(s) 379
Bst4CI ACNGT 3 cut(s) 22, 133, 541
Bst6I CTCTTC 2 cut(s) 114, 576
BstC8I GCNNGC 4 cut(s) 79, 261, 298, 605
BstDEI CTNAG 2 cut(s) 172, 203
BstENI CCTNNNNNAGG 1 cut(s) 233
BstMAI GTCTC 1 cut(s) 191
BstNSI RCATGY 3 cut(s) 220, 328, 607
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 577
BsuRI GGCC 2 cut(s) 296, 575
BtgZI GCGATG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 546, 667
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 4 cut(s) 79, 261, 298, 605
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 2 cut(s) 573, 574
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 273
CviAII CATG 8 cut(s) 155, 160, 217, 325, 413, 487, 517, 604
CviJI RGCY 9 cut(s) 5, 142, 171, 259, 296, 353, 368, 514, 575
CviKI_1 RGCY 9 cut(s) 5, 142, 171, 259, 296, 353, 368, 514, 575
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 2 cut(s) 172, 203
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 114, 576
EarI CTCTTC 2 cut(s) 114, 576
Eco24I GRGCYC 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 647
Eco88I CYCGRG 1 cut(s) 363
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 573, 574
EcoT38I GRGCYC 1 cut(s) 577
FaeI CATG 8 cut(s) 158, 163, 220, 328, 416, 490, 520, 607
FaqI GGGAC 2 cut(s) 142, 360
FatI CATG 8 cut(s) 154, 159, 216, 324, 412, 486, 516, 603
FblI GTMKAC 2 cut(s) 321, 521
FriOI GRGCYC 1 cut(s) 577
FspBI CTAG 2 cut(s) 38, 312
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 2 cut(s) 296, 575
HapII CCGG 2 cut(s) 297, 447
HgaI GACGC 1 cut(s) 41
Hin1II CATG 8 cut(s) 158, 163, 220, 328, 416, 490, 520, 607
HincII GTYRAC 2 cut(s) 18, 399
HindII GTYRAC 2 cut(s) 18, 399
HinfI GANTC 4 cut(s) 14, 44, 199, 359
HpaII CCGG 2 cut(s) 297, 447
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 4 cut(s) 18, 322, 399, 522
Hpy188I TCNGA 2 cut(s) 13, 204
Hpy188III TCNNGA 2 cut(s) 363, 435
Hpy8I GTNNAC 4 cut(s) 18, 322, 399, 522
HpyAV CCTTC 3 cut(s) 293, 580, 622
HpyCH4III ACNGT 3 cut(s) 22, 133, 541
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 3 cut(s) 220, 384, 607
HpyF3I CTNAG 2 cut(s) 172, 203
HpySE526I ACGT 1 cut(s) 567
Hsp92II CATG 8 cut(s) 158, 163, 220, 328, 416, 490, 520, 607
KpnI GGTACC 1 cut(s) 276
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
LmnI GCTCC 1 cut(s) 147
LweI GCATC 1 cut(s) 641
MaeI CTAG 2 cut(s) 38, 312
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 3 cut(s) 53, 206, 478
MboII GAAGA 2 cut(s) 101, 593
MhlI GDGCHC 1 cut(s) 577
MlyI GAGTC 2 cut(s) 23, 193
MnlI CCTC 7 cut(s) 155, 263, 388, 420, 445, 454, 604
MroNI GCCGGC 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 551
MseI TTAA 1 cut(s) 561
MslI CAYNNNNRTG 5 cut(s) 215, 345, 485, 491, 539
MspI CCGG 2 cut(s) 297, 447
NaeI GCCGGC 1 cut(s) 298
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 8 cut(s) 158, 163, 220, 328, 416, 490, 520, 607
NlaIV GGNNCC 4 cut(s) 143, 274, 574, 575
NmuCI GTSAC 3 cut(s) 53, 206, 478
NspI RCATGY 3 cut(s) 220, 328, 607
PaeI GCATGC 1 cut(s) 607
PaeR7I CTCGAG 1 cut(s) 363
PciI ACATGT 1 cut(s) 324
PdiI GCCGGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 551
PfeI GAWTC 2 cut(s) 44, 359
PflMI CCANNNNNTGG 1 cut(s) 266
PleI GAGTC 2 cut(s) 22, 193
PpsI GAGTC 2 cut(s) 22, 193
PscI ACATGT 1 cut(s) 324
PspN4I GGNNCC 4 cut(s) 143, 274, 574, 575
PspOMI GGGCCC 1 cut(s) 573
PspPI GGNCC 2 cut(s) 573, 574
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 5 cut(s) 215, 345, 485, 491, 539
SaqAI TTAA 1 cut(s) 561
Sau96I GGNCC 2 cut(s) 573, 574
SchI GAGTC 2 cut(s) 23, 193
SduI GDGCHC 1 cut(s) 577
SetI ASST 9 cut(s) 173, 231, 274, 321, 355, 429, 475, 570, 591
SfaNI GCATC 1 cut(s) 641
SfcI CTRYAG 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 363
SlaI CTCGAG 1 cut(s) 363
SmiMI CAYNNNNRTG 5 cut(s) 215, 345, 485, 491, 539
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
SphI GCATGC 1 cut(s) 607
SsiI CCGC 3 cut(s) 343, 390, 422
SspMI CTAG 2 cut(s) 38, 312
TaaI ACNGT 3 cut(s) 22, 133, 541
TaiI ACGT 1 cut(s) 570
TaqI TCGA 1 cut(s) 364
TfiI GAWTC 2 cut(s) 44, 359
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 2 cut(s) 546, 667
TseFI GTSAC 3 cut(s) 53, 206, 478
Tsp45I GTSAC 3 cut(s) 53, 206, 478
TspDTI ATGAA 2 cut(s) 318, 584
TspRI CASTG 2 cut(s) 546, 667
Van91I CCANNNNNTGG 1 cut(s) 266
XagI CCTNNNNNAGG 1 cut(s) 233
XceI RCATGY 3 cut(s) 220, 328, 607
XcmI CCANNNNNNNNNTGG 2 cut(s) 157, 667
XhoI CTCGAG 1 cut(s) 363
XmiI GTMKAC 2 cut(s) 321, 521
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 2 cut(s) 38, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.