Rw1G029470

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000352
Physical Location & Seq
Forward (+)
57196361 .. 57197255
895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029470.1

Sequence Viewer

Length: 723 bp
ATGGCAACCCCTAACTCCGGTGCTGTTAGCCTGAAGCTCTTGATTGATTCTAAGCGACGGAAGGTTCTCTTTGCTGAAGCCGGTAAGGATTTTGTGGATTTTCTTTTCACGCTTCTCTCTTTGCCTGTTGGTACTGTCATTAAGCTTCTTTCCAAGGGTGGGATGGTTGGTAGCTTTGGTAAACTGTATGAGAGTGTTGAAAATCTTGATTATACATATATCCAACCCACAATAAACAAGGATATCCTGCTCAAACCCACGGCACCAGTTGCTGGACCTAATATCCTCAAGTTAGCCGACAACTTTGAATCAAGTGCTCGAAAGGAGCTCTACTTGTGTAGTTGTTCAAATAGTTACACTAAAGCCCACCCGTACGTGACTGAAAACCCCGATGAAATATGTCCATCGTGTTCATACACCATGAACCTCCGAACTACATATGTCGGTCAGCCGACTACCACGGAAGCAATTTCTGGTGCCGGCGCTGCTGGATATGTCAAAGGCGTTGTGACATACATGATAATGGATGATCTGGAGGTGAAGCCCATGTCCACCATTTCAAGCATCACTATGCTTAACAGGTTTAATATCAAGGATGTTGGGGCCCTTGAAGAGAAGGTTGTTAAACTTGGCATGGATAAGGGTGTGAAGCTGTTGAAGGAGTCTCTACAGTCCAAATCTGTCCTAACAAATGTCTTCCTTGGGAAGAAATCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

240

Amino Acids

26.03

Weight (kDa)

8.87

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 238 2.1e-47 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 262, 476
AccB7I CCANNNNNTGG 1 cut(s) 272
AcuI CTGAAG 2 cut(s) 53, 96
AfaI GTAC 2 cut(s) 133, 374
AfiI CCNNNNNNNGG 3 cut(s) 17, 159, 272
AgsI TTSAA 6 cut(s) 200, 308, 348, 561, 611, 658
AluBI AGCT 5 cut(s) 37, 145, 174, 328, 652
AluI AGCT 5 cut(s) 37, 145, 174, 328, 652
Alw21I GWGCWC 2 cut(s) 319, 330
Alw26I GTCTC 1 cut(s) 669
AlwNI CAGNNNCTG 1 cut(s) 272
AoxI GGCC 1 cut(s) 603
ApaI GGGCCC 1 cut(s) 607
ApeKI GCWGC 2 cut(s) 485, 716
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 3 cut(s) 275, 603, 604
AsuHPI GGTGA 1 cut(s) 550
AvaII GGWCC 1 cut(s) 275
BaeGI GKGCMC 1 cut(s) 607
BanI GGYRCC 2 cut(s) 262, 476
BanII GRGCYC 2 cut(s) 330, 607
BbsI GAAGAC 1 cut(s) 688
Bbv12I GWGCWC 2 cut(s) 319, 330
BbvI GCAGC 2 cut(s) 472, 703
BccI CCATC 2 cut(s) 157, 412
BceAI ACGGC 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 668
BfoI RGCGCY 1 cut(s) 486
BisI GCNGC 2 cut(s) 486, 717
BlsI GCNGC 2 cut(s) 487, 718
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 3 cut(s) 275, 603, 604
BmiI GGNNCC 4 cut(s) 264, 478, 604, 605
BmsI GCATC 1 cut(s) 573
BpiI GAAGAC 1 cut(s) 688
BpmI CTGGAG 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 376
BsaJI CCNNGG 4 cut(s) 153, 258, 459, 700
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 159, 272
Bse118I RCCGGY 2 cut(s) 80, 479
Bse1I ACTGG 1 cut(s) 266
BseDI CCNNGG 4 cut(s) 153, 258, 459, 700
BseGI GGATG 3 cut(s) 168, 532, 601
BseLI CCNNNNNNNGG 3 cut(s) 17, 159, 272
BseNI ACTGG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 607
BseXI GCAGC 2 cut(s) 472, 703
BshFI GGCC 1 cut(s) 605
BshNI GGYRCC 2 cut(s) 262, 476
BsiHKAI GWGCWC 2 cut(s) 319, 330
BsiSI CCGG 3 cut(s) 18, 81, 480
BsiWI CGTACG 1 cut(s) 372
BslI CCNNNNNNNGG 3 cut(s) 17, 159, 272
BsmAI GTCTC 1 cut(s) 669
BsnI GGCC 1 cut(s) 605
Bsp120I GGGCCC 1 cut(s) 603
Bsp1286I GDGCHC 3 cut(s) 319, 330, 607
Bsp143I GATC 1 cut(s) 529
BspANI GGCC 1 cut(s) 605
BspLI GGNNCC 4 cut(s) 264, 478, 604, 605
BspT107I GGYRCC 2 cut(s) 262, 476
BsrFI RCCGGY 2 cut(s) 80, 479
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 2 cut(s) 80, 479
BssECI CCNNGG 4 cut(s) 153, 258, 459, 700
BssMI GATC 1 cut(s) 529
BssT1I CCWWGG 2 cut(s) 153, 700
Bst4CI ACNGT 3 cut(s) 136, 186, 672
Bst6I CTCTTC 1 cut(s) 606
BstAPI GCANNNNNTGC 1 cut(s) 269
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 481
BstDEI CTNAG 1 cut(s) 51
BstDSI CCRYGG 2 cut(s) 258, 459
BstF5I GGATG 3 cut(s) 168, 532, 601
BstH2I RGCGCY 1 cut(s) 486
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 1 cut(s) 532
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 1 cut(s) 529
BstMWI GCNNNNNNNGC 2 cut(s) 269, 485
BstSFI CTRYAG 1 cut(s) 668
BstSLI GKGCMC 1 cut(s) 607
BstV1I GCAGC 2 cut(s) 472, 703
BstV2I GAAGAC 1 cut(s) 688
BsuRI GGCC 1 cut(s) 605
BtgI CCRYGG 2 cut(s) 258, 459
BtsCI GGATG 3 cut(s) 168, 532, 601
Cac8I GCNNGC 1 cut(s) 481
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 1 cut(s) 485
Cfr10I RCCGGY 2 cut(s) 80, 479
Cfr13I GGNCC 3 cut(s) 275, 603, 604
Csp6I GTAC 2 cut(s) 132, 373
CviAII CATG 5 cut(s) 421, 517, 547, 634, 720
CviQI GTAC 2 cut(s) 132, 373
DdeI CTNAG 1 cut(s) 51
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 153, 700
Eco24I GRGCYC 2 cut(s) 330, 607
Eco32I GATATC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 2 cut(s) 53, 96
EcoICRI GAGCTC 1 cut(s) 328
EcoO109I RGGNCCY 2 cut(s) 603, 604
EcoRV GATATC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 153, 700
EcoT38I GRGCYC 2 cut(s) 330, 607
ErhI CCWWGG 2 cut(s) 153, 700
FaeI CATG 5 cut(s) 424, 520, 550, 637, 723
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 5 cut(s) 420, 516, 546, 633, 719
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 2 cut(s) 486, 717
FokI GGATG 3 cut(s) 175, 539, 608
FriOI GRGCYC 2 cut(s) 330, 607
Fsp4HI GCNGC 2 cut(s) 486, 717
GlaI GCGC 1 cut(s) 484
GluI GCNGC 2 cut(s) 486, 717
GsuI CTGGAG 1 cut(s) 554
HaeII RGCGCY 1 cut(s) 486
HaeIII GGCC 1 cut(s) 605
HapII CCGG 3 cut(s) 18, 81, 480
HhaI GCGC 1 cut(s) 485
Hin1II CATG 5 cut(s) 424, 520, 550, 637, 723
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 3 cut(s) 47, 308, 662
HpaII CCGG 3 cut(s) 18, 81, 480
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 2 cut(s) 182, 552
Hpy188I TCNGA 1 cut(s) 431
Hpy188III TCNNGA 3 cut(s) 40, 206, 533
Hpy8I GTNNAC 2 cut(s) 182, 552
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 3 cut(s) 55, 610, 652
HpyCH4III ACNGT 3 cut(s) 136, 186, 672
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 1 cut(s) 719
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 485
HpyF3I CTNAG 1 cut(s) 51
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 5 cut(s) 424, 520, 550, 637, 723
HspAI GCGC 1 cut(s) 483
KroI GCCGGC 1 cut(s) 479
KroNI GCCGGC 1 cut(s) 481
Kzo9I GATC 1 cut(s) 529
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 2 cut(s) 472, 703
LweI GCATC 1 cut(s) 573
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 3 cut(s) 353, 376, 508
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 3 cut(s) 623, 688, 718
MhlI GDGCHC 3 cut(s) 319, 330, 607
MluCI AATT 1 cut(s) 468
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 3 cut(s) 296, 437, 529
MroNI GCCGGC 1 cut(s) 479
MseI TTAA 4 cut(s) 141, 576, 585, 624
MslI CAYNNNNRTG 2 cut(s) 521, 569
MspI CCGG 3 cut(s) 18, 81, 480
MwoI GCNNNNNNNGC 2 cut(s) 269, 485
NaeI GCCGGC 1 cut(s) 481
NdeI CATATG 1 cut(s) 439
NdeII GATC 1 cut(s) 529
NgoMIV GCCGGC 1 cut(s) 479
NlaIII CATG 5 cut(s) 424, 520, 550, 637, 723
NlaIV GGNNCC 4 cut(s) 264, 478, 604, 605
NmuCI GTSAC 2 cut(s) 376, 508
PdiI GCCGGC 1 cut(s) 481
PfeI GAWTC 2 cut(s) 47, 308
Pfl23II CGTACG 1 cut(s) 372
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 2 cut(s) 487, 718
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 330
PspLI CGTACG 1 cut(s) 372
PspN4I GGNNCC 4 cut(s) 264, 478, 604, 605
PspOMI GGGCCC 1 cut(s) 603
PspPI GGNCC 3 cut(s) 275, 603, 604
PstNI CAGNNNCTG 1 cut(s) 272
RsaI GTAC 2 cut(s) 133, 374
RsaNI GTAC 2 cut(s) 132, 373
RseI CAYNNNNRTG 2 cut(s) 521, 569
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 4 cut(s) 141, 576, 585, 624
SatI GCNGC 2 cut(s) 486, 717
Sau3AI GATC 1 cut(s) 529
Sau96I GGNCC 3 cut(s) 275, 603, 604
SchI GAGTC 1 cut(s) 671
SduI GDGCHC 3 cut(s) 319, 330, 607
SfaNI GCATC 1 cut(s) 573
SfcI CTRYAG 1 cut(s) 668
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 2 cut(s) 521, 569
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 1 cut(s) 468
SstI GAGCTC 1 cut(s) 330
StyI CCWWGG 2 cut(s) 153, 700
TaaI ACNGT 3 cut(s) 136, 186, 672
TaiI ACGT 1 cut(s) 378
TaqI TCGA 1 cut(s) 319
TaqII GACCGA 1 cut(s) 434
TasI AATT 1 cut(s) 468
TfiI GAWTC 2 cut(s) 47, 308
Tru1I TTAA 4 cut(s) 141, 576, 585, 624
Tru9I TTAA 4 cut(s) 141, 576, 585, 624
TseFI GTSAC 2 cut(s) 376, 508
TseI GCWGC 2 cut(s) 485, 716
Tsp45I GTSAC 2 cut(s) 376, 508
TspDTI ATGAA 3 cut(s) 402, 408, 437
TspGWI ACGGA 2 cut(s) 73, 476
Van91I CCANNNNNTGG 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.