FvH4_5g31830

Protein NRT1 PTR FAMILY 5.10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000191
Physical Location & Seq
Reverse (-)
22956894 .. 22961172
4279 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31830.t1

Sequence Viewer

Length: 1686 bp
ATGGCCACTCCTCCTCAGCCTGACGACGACCTCAAAACCCCGTTGCTCCAAAACGACGTCGAGGCCGCCGTTTTGGACTACAAGCACCCCCCCACCGGCTCCGGCGGCTGGCGCTCCGCCATCTTCATAATCGGTGTTGGATGTACGGAGAGGTTCGCATTCTACGGCGTCAGTTCGAACCTGGTGACGTTTCTGAGCGGGCCGTTGGGGGAGTCGACGGCGACGGCGGCGGAGAATGTGAACACGTGGTCCGGGACCGCGTCTTTGCTTCCGTTGCTCGGCGCCGTTGTCGCCGATTCGTTTCTCGGTCGCTACCGCACCATCGTTTTCTCTTCTCTGCTCTACATCCTGGGGCTAGGCTTGTTGAGTTTCTCGGCTAAGCTACCTTCTCTCACTGGTTCTGATTGCGAAAACAGCAGCCAGTTGACATCATGTTATTCTCAGCTCCAAGTAATATTCTTCTACTTCTCACTATATCTGGTAGCAGTAGGGCAAGGGGGAAACAAACCTTGCATACAAGCTTTCGGAGCTGATCAGTTCGATGGACAAGATCCGGTGGAGAGCAAAGCCAAAAGCTCATTCTTTAATTGGTGGTACTTCGGTATCTGCTCAGGCTCCTTATGCACACGATTGGTATCAAGCTACATACAGGACAACTTCAGTTGGGTTCTGGGATTCGGAATTCCTTGTATTATGATGGCCCTTGGCCTATTTGTTTTCTTGTCCGGATCAAGAACTTATAGTAGTGTCGAAGGGGATGGGAAAAGCCCATATGCTAGAATTGGTAGGGTTTTTGTTGCTGCATTAAAGAATTCACGTACTACTCCTTCAGACCTGCCTCACCAAAGTTCTGAAAAGTTCAAGTTCCTCAACAAGGCATTGCTAGTGCCAAATAGTGAATCGAAGGGCTGTAGCAAGGTCTGTACCGAATCTGAGGTAGAAGAAGCAAAGGTTGTTCTTGGTCTTTGTCCGATATGGTGTACATGTCTGGTGTATGCTATTGTCCTTGCACAGATGTCGACTTTCTTTACCAAGCAAGGGGCAACATTGGACAGAGAAATTGCAGCCGGCTTTGAGATACCAGCTGCTTCACTTCAGTCTTTTACCAGCCTGATTATTATTCTCTTCATTCCTGTTTATGAGCGTATCTTTGTTCCGATGGCAAGTGCTTTCTCTGGAAATCCCACCGGCATTACAACACTGCAAAGAATCGGAACTGGGATATTTTTAGCTGCTCTTTCCATGATAGCTGCAGCTGTCGTTGAGTTTAAAAGGCTTGAAACTGCTCAAGAGTATGCTCTGGTTGATATGCCACATGTTACAATTCCAATGGGCGTGTGGTGGTTGATTCCTCAGTACTTGTTGATTGGAGTGTCTGATGTTTTCGCGATGGTTGGTCTGCAAGAGTTTTTCTACGATCAGGTACCAAATGAACTAAGAAGTGTAGGAGTTGCGCTCTACCTCAGTATCTTTGGTGTGGGAAACTTTTTGAGCAGCTTTCTTATCTCGGCCATAGAGGAAGCAACCAGCGGAGAAGGCCGAGTTAGCTGGTTTTCCAATAACTTGAATCGTGCACATCTTGATTACTTTTACTGGCTGCTCGCTGGACTCAGTGTAGTGGAACTGGCTGTCTTCACATACTTTGCAAAATCTTATCGATATAAAAAGAGAGGTAGTAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

562

Amino Acids

61.22

Weight (kDa)

5.59

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 52 - 498 2.7e-12 Major Facilitator Superfamily
PTR2 PF00854 105 - 515 7.9e-99 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22540
fragaria_vesca FvH4_5g31770 FvH4_5g31770 FvH4_5g31790 FvH4_5g31790 FvH4_5g31800 FvH4_5g31800 FvH4_5g31800 FvH4_5g31830 FvH4_5g31840 FvH4_5g31840 FvH4_6g12331
malus_domestica MD02G1288000.v1.1 MD02G1288200.v1.1 MD03G1076700.v1.1 MD07G1122800.v1.1 MD08G1218300.v1.1 MD15G1406500.v1.1 MD15G1406600.v1.1 MD15G1406700.v1.1
prunus_persica Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1
pyrus_communis pycom05g17900 pycom08g18920 pycom15g36290 pycom15g36300 pycom15g36310
rosa_chinensis RchiOBHm_Chr3g0482421 RchiOBHm_Chr4g0413471 RchiOBHm_Chr7g0227651 RchiOBHm_Chr7g0227661 RchiOBHm_Chr7g0227671 RchiOBHm_Chr7g0227681 RchiOBHm_Chr7g0227701 RchiOBHm_Chr7g0227711 RchiOBHm_Chr7g0227721 RchiOBHm_Chr7g0227731 RchiOBHm_Chr7g0227741 RchiOBHm_Chr7g0227761 RchiOBHm_Chr7g0227781 RchiOBHm_Chr7g0227791 RchiOBHm_Chr7g0227801 RchiOBHm_Chr7g0227861 RchiOBHm_Chr7g0227881
rosa_laevigata RLG00000001610 RLG00000001611 RLG00000001613 RLG00000001617 RLG00000001618 RLG00000001619 RLG00000001622 RLG00000001623 RLG00000001625 RLG00000001627 RLG00000001628 RLG00000001629 RLG00000001630 RLG00000008217
rosa_multiflora Rmu_co8446845.1_g000001 Rmu_co8488273.1_g000001 Rmu_sc0000347.1_g000023 Rmu_sc0000347.1_g000032 Rmu_sc0000347.1_g000038 Rmu_sc0000347.1_g000046 Rmu_sc0000347.1_g000047 Rmu_sc0000347.1_g000049 Rmu_sc0000347.1_g000050 Rmu_sc0000883.1_g000014 Rmu_sc0000883.1_g000015 Rmu_sc0001930.1_g000001 Rmu_sc0001930.1_g000006 Rmu_sc0005026.1_g000009 Rmu_sc0005026.1_g000013 Rmu_sc0005026.1_g000018 Rmu_sc0005026.1_g000021 Rmu_sc0005743.1_g000008 Rmu_sc0005743.1_g000010 Rmu_sc0010635.1_g000001 Rmu_sc0013384.1_g000001 Rmu_sc0013521.1_g000001 Rmu_sc0014985.1_g000003 Rmu_sc0022490.1_g000001 Rmu_sc0025092.1_g000001 Rmu_sc0036408.1_g000001
rosa_roxburghii Rroxscaffold_3G00231820 Rroxscaffold_3G00231860 Rroxscaffold_3G00231890 Rroxscaffold_3G00231910 Rroxscaffold_3G00231930 Rroxscaffold_3G00231940 Rroxscaffold_3G00231960 Rroxscaffold_3G00231970 Rroxscaffold_3G00231980 Rroxscaffold_5G00357370
rosa_rugosa Rorug03G0196300 Rorug04G0120600 Rorug07G0242700 Rorug07G0242800 Rorug07G0242900 Rorug07G0243000 Rorug07G0243100 Rorug07G0243200 Rorug07G0243400 Rorug07G0243600 Rorug07G0243800 Rorug07G0243900
rosa_samantha Rh3AG109500 Rh3AG248000 Rh3BG283000 Rh3CG114700 Rh3CG279600 Rh3DG275400 Rh3DG275500 Rh3DG275800 Rh4AG180200 Rh4BG179400 Rh4DG175400 Rh6AG297600 Rh6CG311000 Rh7AG396200 Rh7AG396300 Rh7AG396400 Rh7AG396600 Rh7AG397000 Rh7AG397100 Rh7BG377800 Rh7BG377900 Rh7BG378000 Rh7BG378100 Rh7BG378300 Rh7BG378400 Rh7BG378500 Rh7BG378800 Rh7BG378900 Rh7DG391900 Rh7DG392000 Rh7DG392300 Rh7DG392400 Rh7DG392700
rosa_wichuraiana Rw3G022320 Rw4G015000 Rw4G015060 Rw7G032940 Rw7G032950 Rw7G032960 Rw7G032970 Rw7G032980 Rw7G032990 Rw7G033000 Rw7G033010 Rw7G033020 Rw7G033030 Rw7G033050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
Acc36I ACCTGC 1 cut(s) 843
Acc65I GGTACC 1 cut(s) 1423
AccB1I GGYRCC 2 cut(s) 281, 1423
AccBSI CCGCTC 1 cut(s) 198
AccI GTMKAC 2 cut(s) 215, 1019
AccII CGCG 2 cut(s) 260, 1388
AccIII TCCGGA 1 cut(s) 725
AciI CCGC 9 cut(s) 66, 105, 117, 198, 227, 230, 258, 316, 1530
AclWI GGATC 2 cut(s) 545, 736
AcoI YGGCCR 2 cut(s) 3, 1509
AcsI RAATTY 2 cut(s) 681, 811
AcuI CTGAAG 3 cut(s) 643, 813, 1079
AcvI CACGTG 1 cut(s) 246
AcyI GRCGYC 3 cut(s) 57, 168, 282
AfaI GTAC 7 cut(s) 145, 596, 820, 925, 982, 1358, 1425
AfiI CCNNNNNNNGG 5 cut(s) 95, 108, 278, 874, 1038
AflIII ACRYGT 3 cut(s) 243, 983, 1315
AgsI TTSAA 3 cut(s) 862, 1280, 1567
AjnI CCWGG 2 cut(s) 180, 348
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
Alw21I GWGCWC 1 cut(s) 1576
Alw44I GTGCAC 1 cut(s) 1572
AlwI GGATC 2 cut(s) 545, 736
Aor13HI TCCGGA 1 cut(s) 725
AoxI GGCC 7 cut(s) 3, 63, 200, 699, 706, 1509, 1537
ApaLI GTGCAC 1 cut(s) 1572
ApeKI GCWGC 9 cut(s) 417, 800, 1064, 1085, 1232, 1250, 1253, 1494, 1597
ApoI RAATTY 2 cut(s) 681, 811
ArsI GACNNNNNNTTYG 2 cut(s) 247, 279
Asp718I GGTACC 1 cut(s) 1423
AspLEI GCGC 3 cut(s) 114, 284, 1456
AspS9I GGNCC 4 cut(s) 200, 249, 255, 700
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 196, 833
AsuII TTCGAA 1 cut(s) 176
AvaII GGWCC 2 cut(s) 249, 255
BaeGI GKGCMC 1 cut(s) 1576
BaeI ACNNNNGTAYC 6 cut(s) 586, 586, 619, 619, 1450, 1483
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 281, 1423
BarI GAAGNNNNNNTAC 2 cut(s) 811, 843
BbrPI CACGTG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 1624
Bbv12I GWGCWC 1 cut(s) 1576
BbvCI CCTCAGC 1 cut(s) 15
BbvI GCAGC 9 cut(s) 429, 787, 1072, 1076, 1219, 1237, 1265, 1506, 1584
BccI CCATC 7 cut(s) 128, 329, 536, 691, 752, 1153, 1384
BceAI ACGGC 6 cut(s) 53, 181, 187, 234, 240, 269
BcgI CGANNNNNNTGC 2 cut(s) 999, 1033
BciT130I CCWGG 2 cut(s) 182, 350
BclI TGATCA 1 cut(s) 532
BcnI CCSGG 1 cut(s) 253
BfaI CTAG 3 cut(s) 356, 777, 884
BfmI CTRYAG 2 cut(s) 910, 1251
BfoI RGCGCY 2 cut(s) 115, 285
BfuAI ACCTGC 1 cut(s) 843
BlpI GCTNAGC 1 cut(s) 378
BmcAI AGTACT 1 cut(s) 1358
Bme1390I CCNGG 3 cut(s) 182, 253, 350
Bme18I GGWCC 2 cut(s) 249, 255
BmgT120I GGNCC 4 cut(s) 200, 249, 255, 700
BmiI GGNNCC 5 cut(s) 100, 256, 283, 616, 1425
BmrFI CCNGG 3 cut(s) 182, 253, 350
BmrI ACTGGG 1 cut(s) 1227
BmuI ACTGGG 1 cut(s) 1227
BpiI GAAGAC 1 cut(s) 1624
BplI GAGNNNNNCTC 2 cut(s) 1440, 1472
Bpu10I CCTNAGC 2 cut(s) 15, 610
Bpu1102I GCTNAGC 1 cut(s) 378
Bpu14I TTCGAA 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 1272
BpuMI CCSGG 1 cut(s) 253
Bsa29I ATCGAT 1 cut(s) 1657
BsaAI YACGTR 2 cut(s) 246, 818
BsaBI GATNNNNATC 1 cut(s) 634
BsaHI GRCGYC 3 cut(s) 57, 168, 282
BsaJI CCNNGG 2 cut(s) 349, 703
BsaWI WCCGGW 2 cut(s) 553, 725
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 108, 278, 874, 1038
Bse118I RCCGGY 3 cut(s) 95, 1067, 1187
Bse1I ACTGG 5 cut(s) 400, 421, 1222, 1598, 1629
Bse3DI GCAATG 1 cut(s) 878
Bse8I GATNNNNATC 1 cut(s) 634
BseAI TCCGGA 1 cut(s) 725
BseBI CCWGG 2 cut(s) 182, 350
BseCI ATCGAT 1 cut(s) 1657
BseDI CCNNGG 2 cut(s) 349, 703
BseGI GGATG 3 cut(s) 146, 345, 763
BseJI GATNNNNATC 1 cut(s) 634
BseLI CCNNNNNNNGG 5 cut(s) 95, 108, 278, 874, 1038
BseMI GCAATG 1 cut(s) 878
BseMII CTCAG 8 cut(s) 29, 185, 455, 624, 924, 1367, 1477, 1624
BseNI ACTGG 5 cut(s) 400, 421, 1222, 1598, 1629
BseSI GKGCMC 1 cut(s) 1576
BseXI GCAGC 9 cut(s) 429, 787, 1072, 1076, 1219, 1237, 1265, 1506, 1584
Bsh1236I CGCG 2 cut(s) 260, 1388
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 7 cut(s) 5, 65, 202, 701, 708, 1511, 1539
BshNI GGYRCC 2 cut(s) 281, 1423
BshVI ATCGAT 1 cut(s) 1657
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 1576
BsiSI CCGG 7 cut(s) 96, 102, 252, 554, 726, 1068, 1188
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 5 cut(s) 95, 108, 278, 874, 1038
BsmFI GGGAC 1 cut(s) 268
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 7 cut(s) 5, 65, 202, 701, 708, 1511, 1539
Bsp119I TTCGAA 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 1576
Bsp13I TCCGGA 1 cut(s) 725
Bsp1407I TGTACA 1 cut(s) 980
Bsp143I GATC 4 cut(s) 532, 550, 728, 1417
Bsp1720I GCTNAGC 1 cut(s) 378
Bsp68I TCGCGA 1 cut(s) 1388
BspACI CCGC 9 cut(s) 66, 105, 117, 198, 227, 230, 258, 316, 1530
BspANI GGCC 7 cut(s) 5, 65, 202, 701, 708, 1511, 1539
BspCNI CTCAG 8 cut(s) 28, 186, 454, 623, 925, 1366, 1476, 1623
BspDI ATCGAT 1 cut(s) 1657
BspEI TCCGGA 1 cut(s) 725
BspFNI CGCG 2 cut(s) 260, 1388
BspLI GGNNCC 5 cut(s) 100, 256, 283, 616, 1425
BspMAI CTGCAG 1 cut(s) 1255
BspMI ACCTGC 1 cut(s) 843
BspPI GGATC 2 cut(s) 545, 736
BspT104I TTCGAA 1 cut(s) 176
BspT107I GGYRCC 2 cut(s) 281, 1423
BsrBI CCGCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 878
BsrFI RCCGGY 3 cut(s) 95, 1067, 1187
BsrGI TGTACA 1 cut(s) 980
BsrI ACTGG 5 cut(s) 400, 421, 1222, 1598, 1629
BssAI RCCGGY 3 cut(s) 95, 1067, 1187
BssECI CCNNGG 2 cut(s) 349, 703
BssMI GATC 4 cut(s) 532, 550, 728, 1417
BssNI GRCGYC 3 cut(s) 57, 168, 282
BssT1I CCWWGG 1 cut(s) 703
Bst2UI CCWGG 2 cut(s) 182, 350
Bst6I CTCTTC 2 cut(s) 337, 1130
BstACI GRCGYC 3 cut(s) 57, 168, 282
BstAUI TGTACA 1 cut(s) 980
BstBAI YACGTR 2 cut(s) 246, 818
BstBI TTCGAA 1 cut(s) 176
BstC8I GCNNGC 4 cut(s) 110, 200, 1069, 1602
BstENI CCTNNNNNAGG 1 cut(s) 872
BstF5I GGATG 3 cut(s) 146, 345, 763
BstFNI CGCG 2 cut(s) 260, 1388
BstH2I RGCGCY 2 cut(s) 115, 285
BstHHI GCGC 3 cut(s) 114, 284, 1456
BstKTI GATC 4 cut(s) 535, 553, 731, 1420
BstMBI GATC 4 cut(s) 532, 550, 728, 1417
BstMCI CGRYCG 1 cut(s) 310
BstNI CCWGG 2 cut(s) 182, 350
BstNSI RCATGY 2 cut(s) 987, 1319
BstSCI CCNGG 3 cut(s) 180, 251, 348
BstSFI CTRYAG 2 cut(s) 910, 1251
BstSLI GKGCMC 1 cut(s) 1576
BstUI CGCG 2 cut(s) 260, 1388
BstV1I GCAGC 9 cut(s) 429, 787, 1072, 1076, 1219, 1237, 1265, 1506, 1584
BstV2I GAAGAC 1 cut(s) 1624
BstX2I RGATCY 1 cut(s) 550
BstYI RGATCY 1 cut(s) 550
Bsu15I ATCGAT 1 cut(s) 1657
BsuRI GGCC 7 cut(s) 5, 65, 202, 701, 708, 1511, 1539
BsuTUI ATCGAT 1 cut(s) 1657
BtgZI GCGATG 1 cut(s) 1403
BtsCI GGATG 3 cut(s) 146, 345, 763
BtsI GCAGTG 1 cut(s) 1199
BtsIMutI CAGTG 3 cut(s) 393, 1199, 1618
BtuMI TCGCGA 1 cut(s) 1388
BveI ACCTGC 1 cut(s) 843
Cac8I GCNNGC 4 cut(s) 110, 200, 1069, 1602
CfoI GCGC 3 cut(s) 114, 284, 1456
Cfr10I RCCGGY 3 cut(s) 95, 1067, 1187
Cfr13I GGNCC 4 cut(s) 200, 249, 255, 700
ClaI ATCGAT 1 cut(s) 1657
CseI GACGC 2 cut(s) 157, 249
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 7 cut(s) 144, 595, 819, 924, 981, 1357, 1424
CviAII CATG 4 cut(s) 432, 984, 1243, 1316
CviQI GTAC 7 cut(s) 144, 595, 819, 924, 981, 1357, 1424
DinI GGCGCC 1 cut(s) 283
DpnI GATC 4 cut(s) 534, 552, 730, 1419
DpnII GATC 4 cut(s) 532, 550, 728, 1417
DraI TTTAAA 1 cut(s) 1270
EaeI YGGCCR 2 cut(s) 3, 1509
Eam1104I CTCTTC 2 cut(s) 337, 1130
EarI CTCTTC 2 cut(s) 337, 1130
EciI GGCGGA 2 cut(s) 106, 245
Eco130I CCWWGG 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 249, 255
Eco57I CTGAAG 3 cut(s) 643, 813, 1079
Eco72I CACGTG 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 872
EcoRI GAATTC 2 cut(s) 681, 811
EcoRII CCWGG 2 cut(s) 180, 348
EcoT14I CCWWGG 1 cut(s) 703
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 703
FaeI CATG 4 cut(s) 435, 987, 1246, 1319
FaqI GGGAC 1 cut(s) 268
FatI CATG 4 cut(s) 431, 983, 1242, 1315
FauI CCCGC 1 cut(s) 191
FauNDI CATATG 1 cut(s) 772
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 2 cut(s) 215, 1019
FokI GGATG 3 cut(s) 153, 332, 770
FspBI CTAG 3 cut(s) 356, 777, 884
GlaI GCGC 3 cut(s) 113, 283, 1455
HaeII RGCGCY 2 cut(s) 115, 285
HaeIII GGCC 7 cut(s) 5, 65, 202, 701, 708, 1511, 1539
HapII CCGG 7 cut(s) 96, 102, 252, 554, 726, 1068, 1188
HgaI GACGC 2 cut(s) 157, 249
HhaI GCGC 3 cut(s) 114, 284, 1456
Hin1I GRCGYC 3 cut(s) 57, 168, 282
Hin1II CATG 4 cut(s) 435, 987, 1246, 1319
Hin6I GCGC 3 cut(s) 112, 282, 1454
HinP1I GCGC 3 cut(s) 112, 282, 1454
HincII GTYRAC 3 cut(s) 216, 426, 1020
HindII GTYRAC 3 cut(s) 216, 426, 1020
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 9 cut(s) 212, 296, 675, 899, 929, 1209, 1348, 1567, 1608
HpaII CCGG 7 cut(s) 96, 102, 252, 554, 726, 1068, 1188
HphI GGTGA 2 cut(s) 196, 833
Hpy166II GTNNAC 6 cut(s) 216, 241, 426, 981, 1020, 1574
Hpy188III TCNNGA 6 cut(s) 726, 732, 1176, 1289, 1387, 1580
Hpy8I GTNNAC 6 cut(s) 216, 241, 426, 981, 1020, 1574
Hpy99I CGWCG 5 cut(s) 29, 59, 62, 220, 226
HpyAV CCTTC 5 cut(s) 396, 746, 837, 898, 1529
HpyCH4IV ACGT 4 cut(s) 57, 188, 245, 817
HpySE526I ACGT 4 cut(s) 57, 188, 245, 817
Hsp92I GRCGYC 3 cut(s) 57, 168, 282
Hsp92II CATG 4 cut(s) 435, 987, 1246, 1319
HspAI GCGC 3 cut(s) 112, 282, 1454
KasI GGCGCC 1 cut(s) 281
Kpn2I TCCGGA 1 cut(s) 725
KpnI GGTACC 1 cut(s) 1427
KroI GCCGGC 1 cut(s) 1067
KroNI GCCGGC 1 cut(s) 1069
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 4 cut(s) 532, 550, 728, 1417
LmnI GCTCC 6 cut(s) 51, 104, 119, 450, 527, 620
Lsp1109I GCAGC 9 cut(s) 429, 787, 1072, 1076, 1219, 1237, 1265, 1506, 1584
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 3 cut(s) 356, 777, 884
MaeII ACGT 4 cut(s) 57, 188, 245, 817
MaeIII GTNAC 2 cut(s) 184, 1318
MalI GATC 4 cut(s) 534, 552, 730, 1419
MbiI CCGCTC 1 cut(s) 198
MboI GATC 4 cut(s) 532, 550, 728, 1417
MboII GAAGA 6 cut(s) 115, 324, 451, 953, 1117, 1624
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 1 cut(s) 1576
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 586, 681, 780, 811, 1059, 1323
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 2 cut(s) 221, 1602
MmeI TCCRAC 1 cut(s) 118
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 725
MroNI GCCGGC 1 cut(s) 1067
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 585, 806, 1269
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 1085, 1256, 1530
MspI CCGG 7 cut(s) 96, 102, 252, 554, 726, 1068, 1188
MspR9I CCNGG 3 cut(s) 182, 253, 350
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 2 cut(s) 182, 350
MvnI CGCG 2 cut(s) 260, 1388
NaeI GCCGGC 1 cut(s) 1069
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 253
NdeI CATATG 1 cut(s) 772
NdeII GATC 4 cut(s) 532, 550, 728, 1417
NgoMIV GCCGGC 1 cut(s) 1067
NlaIII CATG 4 cut(s) 435, 987, 1246, 1319
NlaIV GGNNCC 5 cut(s) 100, 256, 283, 616, 1425
NmeAIII GCCGAG 4 cut(s) 258, 353, 1487, 1565
NmuCI GTSAC 1 cut(s) 184
NruI TCGCGA 1 cut(s) 1388
NspI RCATGY 2 cut(s) 987, 1319
NspV TTCGAA 1 cut(s) 176
PciI ACATGT 2 cut(s) 983, 1315
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 158
PdiI GCCGGC 1 cut(s) 1069
PfeI GAWTC 7 cut(s) 296, 675, 899, 929, 1209, 1348, 1567
PflFI GACNNNGTC 1 cut(s) 259
PfoI TCCNGGA 1 cut(s) 251
PleI GAGTC 2 cut(s) 220, 1602
PluTI GGCGCC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 246
PmlI CACGTG 1 cut(s) 246
PpsI GAGTC 2 cut(s) 220, 1602
Ppu21I YACGTR 2 cut(s) 246, 818
PscI ACATGT 2 cut(s) 983, 1315
Psp6I CCWGG 2 cut(s) 180, 348
PspCI CACGTG 1 cut(s) 246
PspGI CCWGG 2 cut(s) 180, 348
PspN4I GGNNCC 5 cut(s) 100, 256, 283, 616, 1425
PspPI GGNCC 4 cut(s) 200, 249, 255, 700
PstI CTGCAG 1 cut(s) 1255
PsuI RGATCY 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 259
PvuII CAGCTG 2 cut(s) 1085, 1256
RruI TCGCGA 1 cut(s) 1388
RsaI GTAC 7 cut(s) 145, 596, 820, 925, 982, 1358, 1425
RsaNI GTAC 7 cut(s) 144, 595, 819, 924, 981, 1357, 1424
SalI GTCGAC 2 cut(s) 214, 1018
SaqAI TTAA 3 cut(s) 585, 806, 1269
Sau3AI GATC 4 cut(s) 532, 550, 728, 1417
Sau96I GGNCC 4 cut(s) 200, 249, 255, 700
ScaI AGTACT 1 cut(s) 1358
SchI GAGTC 2 cut(s) 221, 1602
ScrFI CCNGG 3 cut(s) 182, 253, 350
SduI GDGCHC 1 cut(s) 1576
SexAI ACCWGGT 1 cut(s) 180
SfcI CTRYAG 2 cut(s) 910, 1251
SfoI GGCGCC 1 cut(s) 283
SfuI TTCGAA 1 cut(s) 176
SinI GGWCC 2 cut(s) 249, 255
SmlI CTYRAG 1 cut(s) 1287
SmoI CTYRAG 1 cut(s) 1287
Sse9I AATT 6 cut(s) 586, 681, 780, 811, 1059, 1323
SsiI CCGC 9 cut(s) 66, 105, 117, 198, 227, 230, 258, 316, 1530
SspDI GGCGCC 1 cut(s) 281
SspI AATATT 1 cut(s) 456
SspMI CTAG 3 cut(s) 356, 777, 884
StyD4I CCNGG 3 cut(s) 180, 251, 348
StyI CCWWGG 1 cut(s) 703
TaiI ACGT 4 cut(s) 60, 191, 248, 820
TaqI TCGA 8 cut(s) 60, 176, 215, 540, 750, 902, 1019, 1657
TaqII GACCGA 1 cut(s) 296
TasI AATT 6 cut(s) 586, 681, 780, 811, 1059, 1323
TatI WGTACW 2 cut(s) 980, 1356
TauI GCSGC 3 cut(s) 68, 108, 230
TfiI GAWTC 7 cut(s) 296, 675, 899, 929, 1209, 1348, 1567
Tru1I TTAA 3 cut(s) 585, 806, 1269
Tru9I TTAA 3 cut(s) 585, 806, 1269
TscAI CASTG 3 cut(s) 400, 1206, 1618
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 9 cut(s) 417, 800, 1064, 1085, 1232, 1250, 1253, 1494, 1597
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 3 cut(s) 115, 1117, 1446
TspGWI ACGGA 2 cut(s) 161, 261
TspRI CASTG 3 cut(s) 400, 1206, 1618
Tth111I GACNNNGTC 1 cut(s) 259
VneI GTGCAC 1 cut(s) 1572
VpaK11BI GGWCC 2 cut(s) 249, 255
XagI CCTNNNNNAGG 1 cut(s) 872
XapI RAATTY 2 cut(s) 681, 811
XceI RCATGY 2 cut(s) 987, 1319
XcmI CCANNNNNNNNNTGG 1 cut(s) 1335
XmiI GTMKAC 2 cut(s) 215, 1019
XspI CTAG 3 cut(s) 356, 777, 884
ZraI GACGTC 1 cut(s) 58
ZrmI AGTACT 1 cut(s) 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.