Rw7G033030

Protein NRT1 PTR FAMILY 5.10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000191
Physical Location & Seq
Reverse (-)
48424282 .. 48427262
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G033030.1

Sequence Viewer

Length: 1689 bp
ATGGCCATTTCTCAGCGTGATGATCCCCTCGACCACAAAACCCCATTGCTCCAAAACGACGTCGAGGCGGCCGTTTTGGACTTCAGAGACTCCTCCTTCCCGAAAAGCTCAGGCGGCTGGCGCTCCGCCGCCTTTATAATCGGGGTGGGATGTGCGGAGAGGTTCGCATTCTATGGCGTCAGCTCCAACCTCGTGACGTTTCTGAGCGGGCCGTTGGGGGAGTCGACAGCTACGGCTGCGGAGAATGTGAACACGTGGTCAGGAACGGCGTCGTTGCTTCCCTTGCTCGGAGCCGTCGTCGCCGATTCGTTTCTCGGTCGCTACCGCACCATCGTTATCTCTTCTCTGCTCTATATCCTGGGGCTAGGCTTGTTGAGTTTCTCGGCCAAGCTTCCCTCTCTCACTGGTTCTGATTGCGGAAACAGCAGTCAGTTGACATCATGCTATTCTCAGGTCCAAGTAATATTCTTCTACTTCTCTCTATATCTGGTAGCTGTAGGGCAAGGTGGAAACAAACCTTGCATACAAGCTTTCGGAGCTGATCAGTTCGATGGACAAGATCCAGTGGAGAGCAAAGCCAAAAGCTCATTCTTTAATTGGTGGTACTTTGGTATCTGCTCAGGCTCCTTGTGTACGCGATTGATATCAAGCTACATACAGGACAACTTCAGTTGGGTTCTGGGATTTGGAATTCCTTGTATTGTGATGGCCCTTGGCCTATTTGTTTTCTTGTCCGGAACAAGAACTTATAGGTACAGCGTTGAAGGGGATGGGAAAAGCCCATATGCCAGAATTGCTAAGGTTTTTGCTGCTGCATTTAAGAATTGGCGAACTACTCCTTCAGAGCCTCTTGCAAACCTGCCTCACCAAAGTTCAGAAAAATACAAGTTCCTCAACAAGGCATTGCTACCCGATAGTGATTTGAAGGATTATAACAAGGTCTGTACTGTCTCTGAGGTAGAAGAAGCAAAGGTTGTTCTTAGTCTATTTCCTATATGGAGTACATGCCTGGTATATGCTATTGTGCTTGCACAGATGTCGACTTTCTTTACCAAGCAAGGGGCGACACTGGACAGAGAAATTGTAGCTGGCTTTGAGATACCAGCTGCTTCACTTCAGTCTTTTACCAGCCTCATTATTATCCTCTTCATTCCTGTTTATGAGCGTGTCTTTGTTCCGATGGCAAGTGCTTTCACTGGAAAACCCTCTGGCATTACAACGCTACAAAGAATCGGAGTTGGGATATTTTTAGCTGCTCTTTCCATGATAGCTGCAGCTGTAGTTGAGATGAAAAGGCTTGAAACTGCTCAAGAGTATGCTCTGGTTGATCTGCCACATGTTACAATTCCAATGGGCGTGTGGTGGTTGATTCCTCAGTACTTGTTGATTGGAGTGTCTGATGTTTTCTCGATGGTTGGTCTGCAAGAGTTTTTCTACGATCAGGTACCAAATGAACTAAGAAGTGTAGGAGTCGCCCTCTACCTCAGTATCTTTGGTGTGGGAAACTTTTTAAGCAGCTTTCTTATCTCGGCCATAGAGGAAGCAACCAGCGGAGTAGGCCGAGTTAGCTGGTTTTCCAATAATCTGAATCGTGCGCATCTTGATTACTTTTACTGGCTACTCGCTGGACTGAGTGTAGTGGAACTGGGTGTCTTCACATACTTCGCAAAATCTTACCGATATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

562

Amino Acids

61.61

Weight (kDa)

5.74

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 55 - 505 1.7e-13 Major Facilitator Superfamily
PTR2 PF00854 108 - 524 3.3e-98 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22540
fragaria_vesca FvH4_5g31770 FvH4_5g31770 FvH4_5g31790 FvH4_5g31790 FvH4_5g31800 FvH4_5g31800 FvH4_5g31800 FvH4_5g31830 FvH4_5g31840 FvH4_5g31840 FvH4_6g12331
malus_domestica MD02G1288000.v1.1 MD02G1288200.v1.1 MD03G1076700.v1.1 MD07G1122800.v1.1 MD08G1218300.v1.1 MD15G1406500.v1.1 MD15G1406600.v1.1 MD15G1406700.v1.1
prunus_persica Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1
pyrus_communis pycom05g17900 pycom08g18920 pycom15g36290 pycom15g36300 pycom15g36310
rosa_chinensis RchiOBHm_Chr3g0482421 RchiOBHm_Chr4g0413471 RchiOBHm_Chr7g0227651 RchiOBHm_Chr7g0227661 RchiOBHm_Chr7g0227671 RchiOBHm_Chr7g0227681 RchiOBHm_Chr7g0227701 RchiOBHm_Chr7g0227711 RchiOBHm_Chr7g0227721 RchiOBHm_Chr7g0227731 RchiOBHm_Chr7g0227741 RchiOBHm_Chr7g0227761 RchiOBHm_Chr7g0227781 RchiOBHm_Chr7g0227791 RchiOBHm_Chr7g0227801 RchiOBHm_Chr7g0227861 RchiOBHm_Chr7g0227881
rosa_laevigata RLG00000001610 RLG00000001611 RLG00000001613 RLG00000001617 RLG00000001618 RLG00000001619 RLG00000001622 RLG00000001623 RLG00000001625 RLG00000001627 RLG00000001628 RLG00000001629 RLG00000001630 RLG00000008217
rosa_multiflora Rmu_co8446845.1_g000001 Rmu_co8488273.1_g000001 Rmu_sc0000347.1_g000023 Rmu_sc0000347.1_g000032 Rmu_sc0000347.1_g000038 Rmu_sc0000347.1_g000046 Rmu_sc0000347.1_g000047 Rmu_sc0000347.1_g000049 Rmu_sc0000347.1_g000050 Rmu_sc0000883.1_g000014 Rmu_sc0000883.1_g000015 Rmu_sc0001930.1_g000001 Rmu_sc0001930.1_g000006 Rmu_sc0005026.1_g000009 Rmu_sc0005026.1_g000013 Rmu_sc0005026.1_g000018 Rmu_sc0005026.1_g000021 Rmu_sc0005743.1_g000008 Rmu_sc0005743.1_g000010 Rmu_sc0010635.1_g000001 Rmu_sc0013384.1_g000001 Rmu_sc0013521.1_g000001 Rmu_sc0014985.1_g000003 Rmu_sc0022490.1_g000001 Rmu_sc0025092.1_g000001 Rmu_sc0036408.1_g000001
rosa_roxburghii Rroxscaffold_3G00231820 Rroxscaffold_3G00231860 Rroxscaffold_3G00231890 Rroxscaffold_3G00231910 Rroxscaffold_3G00231930 Rroxscaffold_3G00231940 Rroxscaffold_3G00231960 Rroxscaffold_3G00231970 Rroxscaffold_3G00231980 Rroxscaffold_5G00357370
rosa_rugosa Rorug03G0196300 Rorug04G0120600 Rorug07G0242700 Rorug07G0242800 Rorug07G0242900 Rorug07G0243000 Rorug07G0243100 Rorug07G0243200 Rorug07G0243400 Rorug07G0243600 Rorug07G0243800 Rorug07G0243900
rosa_samantha Rh3AG109500 Rh3AG248000 Rh3BG283000 Rh3CG114700 Rh3CG279600 Rh3DG275400 Rh3DG275500 Rh3DG275800 Rh4AG180200 Rh4BG179400 Rh4DG175400 Rh6AG297600 Rh6CG311000 Rh7AG396200 Rh7AG396300 Rh7AG396400 Rh7AG396600 Rh7AG397000 Rh7AG397100 Rh7BG377800 Rh7BG377900 Rh7BG378000 Rh7BG378100 Rh7BG378300 Rh7BG378400 Rh7BG378500 Rh7BG378800 Rh7BG378900 Rh7DG391900 Rh7DG392000 Rh7DG392300 Rh7DG392400 Rh7DG392700
rosa_wichuraiana Rw3G022320 Rw4G015000 Rw4G015060 Rw7G032940 Rw7G032950 Rw7G032960 Rw7G032970 Rw7G032980 Rw7G032990 Rw7G033000 Rw7G033010 Rw7G033020 Rw7G033030 Rw7G033050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 137, 933
AatII GACGTC 1 cut(s) 63
Acc16I TGCGCA 1 cut(s) 1596
Acc36I ACCTGC 1 cut(s) 867
Acc65I GGTACC 1 cut(s) 1444
AccB1I GGYRCC 1 cut(s) 1444
AccBSI CCGCTC 1 cut(s) 207
AccI GTMKAC 2 cut(s) 224, 1040
AccII CGCG 1 cut(s) 637
AccIII TCCGGA 1 cut(s) 734
AclWI GGATC 2 cut(s) 17, 554
AcoI YGGCCR 4 cut(s) 3, 69, 384, 1530
AcsI RAATTY 1 cut(s) 690
AcuI CTGAAG 4 cut(s) 67, 652, 825, 1100
AcvI CACGTG 1 cut(s) 255
AcyI GRCGYC 3 cut(s) 60, 177, 269
AfaI GTAC 7 cut(s) 605, 634, 755, 946, 1003, 1379, 1446
AfiI CCNNNNNNNGG 3 cut(s) 287, 898, 1059
AflIII ACRYGT 2 cut(s) 252, 1336
AgsI TTSAA 3 cut(s) 764, 925, 1301
AjnI CCWGG 2 cut(s) 357, 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
Alw26I GTCTC 2 cut(s) 81, 955
AlwI GGATC 2 cut(s) 17, 554
Aor13HI TCCGGA 1 cut(s) 734
AoxI GGCC 8 cut(s) 3, 69, 209, 384, 708, 715, 1530, 1558
ApeKI GCWGC 8 cut(s) 236, 809, 812, 1106, 1253, 1271, 1274, 1515
ApoI RAATTY 1 cut(s) 690
Asp718I GGTACC 1 cut(s) 1444
AspLEI GCGC 2 cut(s) 123, 1597
AspS9I GGNCC 3 cut(s) 209, 454, 709
AsuHPI GGTGA 1 cut(s) 857
AvaII GGWCC 1 cut(s) 454
BaeI ACNNNNGTAYC 6 cut(s) 595, 595, 628, 628, 1471, 1504
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1444
BauI CACGAG 1 cut(s) 191
BbrPI CACGTG 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 1645
BbvI GCAGC 8 cut(s) 223, 796, 799, 1093, 1240, 1258, 1286, 1527
BccI CCATC 6 cut(s) 338, 545, 700, 764, 1174, 1405
BceAI ACGGC 5 cut(s) 56, 196, 249, 278, 282
BcgI CGANNNNNNTGC 2 cut(s) 1020, 1054
BciT130I CCWGG 2 cut(s) 359, 1010
BclI TGATCA 1 cut(s) 541
BcoDI GTCTC 2 cut(s) 81, 955
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 3 cut(s) 495, 1272, 1278
BfoI RGCGCY 1 cut(s) 124
BfuAI ACCTGC 1 cut(s) 867
BmcAI AGTACT 1 cut(s) 1379
Bme1390I CCNGG 2 cut(s) 359, 1010
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 3 cut(s) 209, 454, 709
BmiI GGNNCC 3 cut(s) 292, 625, 1446
BmrFI CCNGG 2 cut(s) 359, 1010
BmrI ACTGGG 1 cut(s) 1655
BmsI GCATC 1 cut(s) 1606
BmuI ACTGGG 1 cut(s) 1655
BpiI GAAGAC 1 cut(s) 1645
BplI GAGNNNNNCTC 2 cut(s) 1461, 1493
Bpu10I CCTNAGC 3 cut(s) 109, 619, 798
BpuEI CTTGAG 1 cut(s) 1293
BsaAI YACGTR 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 643
BsaHI GRCGYC 3 cut(s) 60, 177, 269
BsaJI CCNNGG 2 cut(s) 358, 712
BsaWI WCCGGW 1 cut(s) 734
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 898, 1059
Bse1I ACTGG 6 cut(s) 409, 563, 1074, 1201, 1619, 1650
Bse3DI GCAATG 2 cut(s) 44, 902
Bse8I GATNNNNATC 1 cut(s) 643
BseAI TCCGGA 1 cut(s) 734
BseBI CCWGG 2 cut(s) 359, 1010
BseDI CCNNGG 2 cut(s) 358, 712
BseGI GGATG 2 cut(s) 155, 775
BseJI GATNNNNATC 1 cut(s) 643
BseLI CCNNNNNNNGG 3 cut(s) 287, 898, 1059
BseMI GCAATG 2 cut(s) 44, 902
BseMII CTCAG 9 cut(s) 26, 123, 194, 464, 633, 945, 1388, 1498, 1622
BseNI ACTGG 6 cut(s) 409, 563, 1074, 1201, 1619, 1650
BseRI GAGGAG 1 cut(s) 82
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 8 cut(s) 223, 796, 799, 1093, 1240, 1258, 1286, 1527
Bsh1236I CGCG 1 cut(s) 637
Bsh1285I CGRYCG 2 cut(s) 72, 319
BshFI GGCC 8 cut(s) 5, 71, 211, 386, 710, 717, 1532, 1560
BshNI GGYRCC 1 cut(s) 1444
BsiEI CGRYCG 2 cut(s) 72, 319
BsiSI CCGG 1 cut(s) 735
BslI CCNNNNNNNGG 3 cut(s) 287, 898, 1059
BsmAI GTCTC 2 cut(s) 81, 955
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 8 cut(s) 5, 71, 211, 386, 710, 717, 1532, 1560
Bsp13I TCCGGA 1 cut(s) 734
Bsp143I GATC 5 cut(s) 22, 541, 559, 1327, 1438
BspANI GGCC 8 cut(s) 5, 71, 211, 386, 710, 717, 1532, 1560
BspCNI CTCAG 9 cut(s) 25, 122, 195, 463, 632, 946, 1387, 1497, 1623
BspEI TCCGGA 1 cut(s) 734
BspFNI CGCG 1 cut(s) 637
BspLI GGNNCC 3 cut(s) 292, 625, 1446
BspMAI CTGCAG 1 cut(s) 1276
BspMI ACCTGC 1 cut(s) 867
BspPI GGATC 2 cut(s) 17, 554
BspT107I GGYRCC 1 cut(s) 1444
BsrBI CCGCTC 1 cut(s) 207
BsrDI GCAATG 2 cut(s) 44, 902
BsrI ACTGG 6 cut(s) 409, 563, 1074, 1201, 1619, 1650
BssECI CCNNGG 2 cut(s) 358, 712
BssMI GATC 5 cut(s) 22, 541, 559, 1327, 1438
BssNI GRCGYC 3 cut(s) 60, 177, 269
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 712
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 2 cut(s) 359, 1010
Bst4CI ACNGT 1 cut(s) 949
Bst6I CTCTTC 2 cut(s) 346, 1151
BstACI GRCGYC 3 cut(s) 60, 177, 269
BstBAI YACGTR 1 cut(s) 255
BstC8I GCNNGC 4 cut(s) 119, 209, 1029, 1090
BstENI CCTNNNNNAGG 1 cut(s) 896
BstF5I GGATG 2 cut(s) 155, 775
BstFNI CGCG 1 cut(s) 637
BstH2I RGCGCY 1 cut(s) 124
BstHHI GCGC 2 cut(s) 123, 1597
BstKTI GATC 5 cut(s) 25, 544, 562, 1330, 1441
BstMAI GTCTC 2 cut(s) 81, 955
BstMBI GATC 5 cut(s) 22, 541, 559, 1327, 1438
BstMCI CGRYCG 2 cut(s) 72, 319
BstNI CCWGG 2 cut(s) 359, 1010
BstNSI RCATGY 2 cut(s) 1008, 1340
BstSCI CCNGG 2 cut(s) 357, 1008
BstSFI CTRYAG 3 cut(s) 495, 1272, 1278
BstUI CGCG 1 cut(s) 637
BstV1I GCAGC 8 cut(s) 223, 796, 799, 1093, 1240, 1258, 1286, 1527
BstV2I GAAGAC 1 cut(s) 1645
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BstZI CGGCCG 1 cut(s) 69
BsuRI GGCC 8 cut(s) 5, 71, 211, 386, 710, 717, 1532, 1560
BtsCI GGATG 2 cut(s) 155, 775
BtsIMutI CAGTG 4 cut(s) 402, 570, 1067, 1194
BveI ACCTGC 1 cut(s) 867
Cac8I GCNNGC 4 cut(s) 119, 209, 1029, 1090
CfoI GCGC 2 cut(s) 123, 1597
Cfr13I GGNCC 3 cut(s) 209, 454, 709
CseI GACGC 2 cut(s) 166, 258
Csp6I GTAC 7 cut(s) 604, 633, 754, 945, 1002, 1378, 1445
CviAII CATG 4 cut(s) 441, 1005, 1264, 1337
CviQI GTAC 7 cut(s) 604, 633, 754, 945, 1002, 1378, 1445
DpnI GATC 5 cut(s) 24, 543, 561, 1329, 1440
DpnII GATC 5 cut(s) 22, 541, 559, 1327, 1438
EaeI YGGCCR 4 cut(s) 3, 69, 384, 1530
EagI CGGCCG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 346, 1151
EarI CTCTTC 2 cut(s) 346, 1151
EciI GGCGGA 1 cut(s) 115
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 712
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 1 cut(s) 454
Eco52I CGGCCG 1 cut(s) 69
Eco57I CTGAAG 4 cut(s) 67, 652, 825, 1100
Eco72I CACGTG 1 cut(s) 255
EcoNI CCTNNNNNAGG 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 690
EcoRII CCWGG 2 cut(s) 357, 1008
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 712
ErhI CCWWGG 1 cut(s) 712
FaeI CATG 4 cut(s) 444, 1008, 1267, 1340
FatI CATG 4 cut(s) 440, 1004, 1263, 1336
FauI CCCGC 1 cut(s) 200
FauNDI CATATG 1 cut(s) 784
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 2 cut(s) 224, 1040
FokI GGATG 2 cut(s) 162, 782
FspAI RTGCGCAY 1 cut(s) 1596
FspBI CTAG 1 cut(s) 365
FspI TGCGCA 1 cut(s) 1596
GlaI GCGC 2 cut(s) 122, 1596
HaeII RGCGCY 1 cut(s) 124
HaeIII GGCC 8 cut(s) 5, 71, 211, 386, 710, 717, 1532, 1560
HapII CCGG 1 cut(s) 735
HgaI GACGC 2 cut(s) 166, 258
HhaI GCGC 2 cut(s) 123, 1597
Hin1I GRCGYC 3 cut(s) 60, 177, 269
Hin1II CATG 4 cut(s) 444, 1008, 1267, 1340
Hin6I GCGC 2 cut(s) 121, 1595
HinP1I GCGC 2 cut(s) 121, 1595
HincII GTYRAC 3 cut(s) 225, 435, 1041
HindII GTYRAC 3 cut(s) 225, 435, 1041
HindIII AAGCTT 2 cut(s) 389, 528
HinfI GANTC 7 cut(s) 89, 221, 305, 1230, 1369, 1470, 1588
HpaII CCGG 1 cut(s) 735
HphI GGTGA 1 cut(s) 857
Hpy166II GTNNAC 5 cut(s) 225, 250, 435, 633, 1041
Hpy188III TCNNGA 7 cut(s) 100, 193, 261, 735, 1310, 1408, 1601
Hpy8I GTNNAC 5 cut(s) 225, 250, 435, 633, 1041
Hpy99I CGWCG 5 cut(s) 62, 65, 274, 299, 302
HpyAV CCTTC 4 cut(s) 106, 758, 849, 919
HpyCH4III ACNGT 1 cut(s) 949
HpyCH4IV ACGT 3 cut(s) 60, 197, 254
HpyCH4V TGCA 6 cut(s) 522, 815, 854, 1031, 1274, 1423
HpySE526I ACGT 3 cut(s) 60, 197, 254
Hsp92I GRCGYC 3 cut(s) 60, 177, 269
Hsp92II CATG 4 cut(s) 444, 1008, 1267, 1340
HspAI GCGC 2 cut(s) 121, 1595
Kpn2I TCCGGA 1 cut(s) 734
KpnI GGTACC 1 cut(s) 1448
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 5 cut(s) 22, 541, 559, 1327, 1438
LmnI GCTCC 6 cut(s) 54, 128, 188, 290, 536, 629
Lsp1109I GCAGC 8 cut(s) 223, 796, 799, 1093, 1240, 1258, 1286, 1527
LweI GCATC 1 cut(s) 1606
MaeI CTAG 1 cut(s) 365
MaeII ACGT 3 cut(s) 60, 197, 254
MaeIII GTNAC 2 cut(s) 193, 1339
MalI GATC 5 cut(s) 24, 543, 561, 1329, 1440
MbiI CCGCTC 1 cut(s) 207
MboI GATC 5 cut(s) 22, 541, 559, 1327, 1438
MboII GAAGA 5 cut(s) 333, 460, 974, 1138, 1645
MflI RGATCY 1 cut(s) 559
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 595, 690, 792, 823, 1080, 1344
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 83, 230, 1479
MmeI TCCRAC 1 cut(s) 210
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 734
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 594, 819, 1511
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 1106, 1277, 1551
MspI CCGG 1 cut(s) 735
MspR9I CCNGG 2 cut(s) 359, 1010
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 2 cut(s) 359, 1010
MvnI CGCG 1 cut(s) 637
NdeI CATATG 1 cut(s) 784
NdeII GATC 5 cut(s) 22, 541, 559, 1327, 1438
NlaIII CATG 4 cut(s) 444, 1008, 1267, 1340
NlaIV GGNNCC 3 cut(s) 292, 625, 1446
NmeAIII GCCGAG 3 cut(s) 362, 1508, 1586
NmuCI GTSAC 1 cut(s) 193
NsbI TGCGCA 1 cut(s) 1596
NspI RCATGY 2 cut(s) 1008, 1340
PciI ACATGT 1 cut(s) 1336
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 294
PctI GAATGC 1 cut(s) 167
PfeI GAWTC 4 cut(s) 305, 1230, 1369, 1588
PleI GAGTC 3 cut(s) 83, 229, 1478
PmaCI CACGTG 1 cut(s) 255
PmlI CACGTG 1 cut(s) 255
PpsI GAGTC 3 cut(s) 83, 229, 1478
Ppu21I YACGTR 1 cut(s) 255
PscI ACATGT 1 cut(s) 1336
PsiI TTATAA 2 cut(s) 137, 933
Psp6I CCWGG 2 cut(s) 357, 1008
PspCI CACGTG 1 cut(s) 255
PspGI CCWGG 2 cut(s) 357, 1008
PspN4I GGNNCC 3 cut(s) 292, 625, 1446
PspPI GGNCC 3 cut(s) 209, 454, 709
PstI CTGCAG 1 cut(s) 1276
PsuI RGATCY 1 cut(s) 559
PvuII CAGCTG 2 cut(s) 1106, 1277
RsaI GTAC 7 cut(s) 605, 634, 755, 946, 1003, 1379, 1446
RsaNI GTAC 7 cut(s) 604, 633, 754, 945, 1002, 1378, 1445
SalI GTCGAC 2 cut(s) 223, 1039
SaqAI TTAA 3 cut(s) 594, 819, 1511
Sau3AI GATC 5 cut(s) 22, 541, 559, 1327, 1438
Sau96I GGNCC 3 cut(s) 209, 454, 709
ScaI AGTACT 1 cut(s) 1379
SchI GAGTC 3 cut(s) 83, 230, 1479
ScrFI CCNGG 2 cut(s) 359, 1010
SfaNI GCATC 1 cut(s) 1606
SfcI CTRYAG 3 cut(s) 495, 1272, 1278
SinI GGWCC 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 1308
SmoI CTYRAG 1 cut(s) 1308
Sse9I AATT 6 cut(s) 595, 690, 792, 823, 1080, 1344
SspI AATATT 1 cut(s) 465
SspMI CTAG 1 cut(s) 365
StyD4I CCNGG 2 cut(s) 357, 1008
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 1 cut(s) 949
TaiI ACGT 3 cut(s) 63, 200, 257
TaqI TCGA 6 cut(s) 30, 63, 224, 549, 1040, 1409
TaqII GACCGA 1 cut(s) 305
TasI AATT 6 cut(s) 595, 690, 792, 823, 1080, 1344
TatI WGTACW 3 cut(s) 944, 1001, 1377
TauI GCSGC 3 cut(s) 71, 117, 131
TfiI GAWTC 4 cut(s) 305, 1230, 1369, 1588
Tru1I TTAA 3 cut(s) 594, 819, 1511
Tru9I TTAA 3 cut(s) 594, 819, 1511
TscAI CASTG 4 cut(s) 409, 570, 1074, 1201
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 8 cut(s) 236, 809, 812, 1106, 1253, 1271, 1274, 1515
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 3 cut(s) 1138, 1304, 1467
TspRI CASTG 4 cut(s) 409, 570, 1074, 1201
VpaK11BI GGWCC 1 cut(s) 454
XagI CCTNNNNNAGG 1 cut(s) 896
XapI RAATTY 1 cut(s) 690
XceI RCATGY 2 cut(s) 1008, 1340
XcmI CCANNNNNNNNNTGG 1 cut(s) 1356
XmiI GTMKAC 2 cut(s) 224, 1040
XspI CTAG 1 cut(s) 365
ZraI GACGTC 1 cut(s) 61
ZrmI AGTACT 1 cut(s) 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.