Rroxscaffold_3G00231960

Protein NRT1 PTR FAMILY 5.10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000191
Physical Location & Seq
Forward (+)
17525817 .. 17529803
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231960.1

Sequence Viewer

Length: 1809 bp
ATGGCTACATCTCTCGAAGAATATCCGGAGACCCGAACTCCACTCCTAGACGACGTCGTAGACGGCGCCGTTGATTACAAAGGCCATCAGGTCCACAGATCAACCTCCGGCGGTTGGCATTCCGCATCCTTCATAATAGGAGTGGAAGTTGCGGAGAGGTCTGCCTACTATGGAATAAGCTCGAATCTGATAACGTTTCTGACGGGGCCGTTGGGACAGTCGACGGCGACGGCGGCGGAGAATGTGAACATATGGTTCGGAACGGCGTCGTTGCTTCCTCTATTGGGAGCGTTCGTGGCGGACTGTTTTCTTGGACGCTACCGCACCATTATTGCTGCTTCTCTCCTTTACATTTTGCATGCGGTTTTTTTTGTCTTTTTGCATAGCAATGCGATCCTTAGAAAGAACACTAGCCTAGCATCTAGCTGCTTTGTCATGGATGAGAAGGAGAAATCCGAGAAATGGAACTTAGTTGAAGTGTTCCCTTTTGGCCAGGGACTCGGCTTGTTGACCGTGTCGGCCTTGCTTACTTCTTCTGAGCTTCAAATATTGTTATTCTTCTTCTCTCTATATCTAGTAGCAGTTGCACAAGGTGGACACAAGCCCTGTGTTCAGGCTTTTGGGGCAGATCAGTTTGATGGACAAGATCCAGAGGAGTGCAAAGCAAAAAGCTCATTCTTCAATTGGTGGTACTTTGGCATGTGTACAGGCTCGCTATTCACAGTCTTGGTATTGGCCTACATACAGGACAATCTAAGCTGGGGTCTGGGTTTTGGAATTCCATGTGTTGTGATGGTCCTTGCACTGATTATTTTTATCAGTGGAACTAGAACATACCGGTATAGCATCAAAGGGGAGGAGGAAAGCCCATTTGTGAGAATAGGCAAGGTTTTTGTTGCTGCATTAAGGAACCGGCGAACTGTTTCTTCAGCAATAGCTTCTGAAGAAGAATCCTGTGGAACCTTGGCTGATCAAAGTTCTGAACAATTCAAGTTCCTCAACAAAGCCTTGCTTGCACCAGATGATTTGAAGAAAAACAGGAAGGTGTGTACCATCGCTGAGGTTGAGGAAGCAAAGGCTGTTCTGCGGCTGTTTCCAATATGGGCTACATGCTTGGCATATGCTATTGTATTTGCACAGTGCACTACTTTCTTCACCAAGCAAGGTGCCACCATGGACAGAACTATTGTCCCCGGTTTCGACATACCAGCTGCTTCACTTCAATCATTTATCAGCCTTGCCATAATGATAGTCATACCCATGTATGACTGCATTTTTGTACCAATGGCAAGAGCTTTCACTAGAGAACCCTCTGGCATTACAATGCTACAAAGAATCGGAACTGGGATGTTTTTCTCTATCATTTCTATGGTTGTTGCAGCTTTAGTTGAGATAAAAAGGCTCCAAACTGCAAAAGATTATGATCTGGTTGATCTGCCAAGTGCCACAATTCCAATGAGTATTTGGTGGTTAGTTCCTCAATACTTCTTGTATGGACTGGCCGATGCTTTCACCATGGTTGGTCTACAAGAGTTCTTCTACGATCAGGTACCAAATGAATTAAGAAGCGTAGGACTTGCCCTCTACCTCAGTATATTTGGTGTGGGAAGCTTTCTGAGCAGCTTTCTTATCTCTATTATTGAGGAAGCAACCAGTTGGGCAGGTCACACTAGCTGGTTTTCCGATAACCTTAATCTTGCGCATCTTGATTACTTTTACTGGTTACTTGCAGCACTCAGTGCAGTAGGATTGTTGGCCTACTTGTATTTTGCAAAATCCTACATTTATAATAGGGCTAGTACAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

602

Amino Acids

66.67

Weight (kDa)

5.33

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 136 - 559 7.7e-92 POT family
MFS_1 PF07690 160 - 540 1.2e-12 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22540
fragaria_vesca FvH4_5g31770 FvH4_5g31770 FvH4_5g31790 FvH4_5g31790 FvH4_5g31800 FvH4_5g31800 FvH4_5g31800 FvH4_5g31830 FvH4_5g31840 FvH4_5g31840 FvH4_6g12331
malus_domestica MD02G1288000.v1.1 MD02G1288200.v1.1 MD03G1076700.v1.1 MD07G1122800.v1.1 MD08G1218300.v1.1 MD15G1406500.v1.1 MD15G1406600.v1.1 MD15G1406700.v1.1
prunus_persica Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1
pyrus_communis pycom05g17900 pycom08g18920 pycom15g36290 pycom15g36300 pycom15g36310
rosa_chinensis RchiOBHm_Chr3g0482421 RchiOBHm_Chr4g0413471 RchiOBHm_Chr7g0227651 RchiOBHm_Chr7g0227661 RchiOBHm_Chr7g0227671 RchiOBHm_Chr7g0227681 RchiOBHm_Chr7g0227701 RchiOBHm_Chr7g0227711 RchiOBHm_Chr7g0227721 RchiOBHm_Chr7g0227731 RchiOBHm_Chr7g0227741 RchiOBHm_Chr7g0227761 RchiOBHm_Chr7g0227781 RchiOBHm_Chr7g0227791 RchiOBHm_Chr7g0227801 RchiOBHm_Chr7g0227861 RchiOBHm_Chr7g0227881
rosa_laevigata RLG00000001610 RLG00000001611 RLG00000001613 RLG00000001617 RLG00000001618 RLG00000001619 RLG00000001622 RLG00000001623 RLG00000001625 RLG00000001627 RLG00000001628 RLG00000001629 RLG00000001630 RLG00000008217
rosa_multiflora Rmu_co8446845.1_g000001 Rmu_co8488273.1_g000001 Rmu_sc0000347.1_g000023 Rmu_sc0000347.1_g000032 Rmu_sc0000347.1_g000038 Rmu_sc0000347.1_g000046 Rmu_sc0000347.1_g000047 Rmu_sc0000347.1_g000049 Rmu_sc0000347.1_g000050 Rmu_sc0000883.1_g000014 Rmu_sc0000883.1_g000015 Rmu_sc0001930.1_g000001 Rmu_sc0001930.1_g000006 Rmu_sc0005026.1_g000009 Rmu_sc0005026.1_g000013 Rmu_sc0005026.1_g000018 Rmu_sc0005026.1_g000021 Rmu_sc0005743.1_g000008 Rmu_sc0005743.1_g000010 Rmu_sc0010635.1_g000001 Rmu_sc0013384.1_g000001 Rmu_sc0013521.1_g000001 Rmu_sc0014985.1_g000003 Rmu_sc0022490.1_g000001 Rmu_sc0025092.1_g000001 Rmu_sc0036408.1_g000001
rosa_roxburghii Rroxscaffold_3G00231820 Rroxscaffold_3G00231860 Rroxscaffold_3G00231890 Rroxscaffold_3G00231910 Rroxscaffold_3G00231930 Rroxscaffold_3G00231940 Rroxscaffold_3G00231960 Rroxscaffold_3G00231970 Rroxscaffold_3G00231980 Rroxscaffold_5G00357370
rosa_rugosa Rorug03G0196300 Rorug04G0120600 Rorug07G0242700 Rorug07G0242800 Rorug07G0242900 Rorug07G0243000 Rorug07G0243100 Rorug07G0243200 Rorug07G0243400 Rorug07G0243600 Rorug07G0243800 Rorug07G0243900
rosa_samantha Rh3AG109500 Rh3AG248000 Rh3BG283000 Rh3CG114700 Rh3CG279600 Rh3DG275400 Rh3DG275500 Rh3DG275800 Rh4AG180200 Rh4BG179400 Rh4DG175400 Rh6AG297600 Rh6CG311000 Rh7AG396200 Rh7AG396300 Rh7AG396400 Rh7AG396600 Rh7AG397000 Rh7AG397100 Rh7BG377800 Rh7BG377900 Rh7BG378000 Rh7BG378100 Rh7BG378300 Rh7BG378400 Rh7BG378500 Rh7BG378800 Rh7BG378900 Rh7DG391900 Rh7DG392000 Rh7DG392300 Rh7DG392400 Rh7DG392700
rosa_wichuraiana Rw3G022320 Rw4G015000 Rw4G015060 Rw7G032940 Rw7G032950 Rw7G032960 Rw7G032970 Rw7G032980 Rw7G032990 Rw7G033000 Rw7G033010 Rw7G033020 Rw7G033030 Rw7G033050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1788
AatII GACGTC 1 cut(s) 57
Acc16I TGCGCA 1 cut(s) 1701
Acc36I ACCTGC 1 cut(s) 1652
Acc65I GGTACC 1 cut(s) 1549
AccB1I GGYRCC 3 cut(s) 65, 1166, 1549
AccI GTMKAC 3 cut(s) 60, 221, 1525
AccIII TCCGGA 1 cut(s) 25
AciI CCGC 9 cut(s) 111, 123, 152, 233, 236, 299, 322, 362, 1087
AclI AACGTT 1 cut(s) 194
AclWI GGATC 2 cut(s) 388, 641
AcoI YGGCCR 2 cut(s) 490, 1500
AcsI RAATTY 1 cut(s) 777
AcuI CTGAAG 2 cut(s) 912, 963
AcyI GRCGYC 3 cut(s) 54, 66, 266
AdeI CACNNNGTG 2 cut(s) 593, 1739
AfaI GTAC 6 cut(s) 692, 706, 1051, 1281, 1551, 1801
AfiI CCNNNNNNNGG 3 cut(s) 114, 284, 462
AgeI ACCGGT 1 cut(s) 837
AgsI TTSAA 6 cut(s) 476, 545, 682, 991, 1030, 1223
AjnI CCWGG 1 cut(s) 492
Alw21I GWGCWC 1 cut(s) 1145
Alw26I GTCTC 1 cut(s) 23
Alw44I GTGCAC 1 cut(s) 1141
AlwI GGATC 2 cut(s) 388, 641
Aor13HI TCCGGA 1 cut(s) 25
AoxI GGCC 7 cut(s) 82, 206, 490, 519, 735, 1500, 1755
ApaLI GTGCAC 1 cut(s) 1141
ApeKI GCWGC 7 cut(s) 335, 426, 899, 1211, 1379, 1620, 1730
ApoI RAATTY 1 cut(s) 777
ArsI GACNNNNNNTTYG 2 cut(s) 1259, 1291
AsiGI ACCGGT 1 cut(s) 837
Asp700I GAANNNNTTC 1 cut(s) 922
Asp718I GGTACC 1 cut(s) 1549
AspLEI GCGC 2 cut(s) 68, 1702
AspS9I GGNCC 3 cut(s) 91, 206, 796
AsuC2I CCSGG 1 cut(s) 1194
AsuHPI GGTGA 2 cut(s) 1147, 1504
AvaII GGWCC 2 cut(s) 91, 796
BaeGI GKGCMC 1 cut(s) 1145
BalI TGGCCA 1 cut(s) 492
BanI GGYRCC 3 cut(s) 65, 1166, 1549
Bbv12I GWGCWC 1 cut(s) 1145
BbvCI CCTCAGC 1 cut(s) 1059
BbvI GCAGC 7 cut(s) 322, 413, 886, 1198, 1391, 1632, 1742
BccI CCATC 4 cut(s) 93, 632, 787, 1061
BceAI ACGGC 6 cut(s) 53, 79, 193, 240, 246, 279
BciT130I CCWGG 1 cut(s) 494
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 1 cut(s) 1194
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 9 cut(s) 47, 411, 416, 423, 575, 828, 1302, 1671, 1797
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 1652
BisI GCNGC 9 cut(s) 234, 336, 427, 900, 1088, 1212, 1380, 1621, 1731
BlsI GCNGC 9 cut(s) 235, 337, 428, 901, 1089, 1213, 1381, 1622, 1732
Bme1390I CCNGG 2 cut(s) 494, 1194
Bme18I GGWCC 2 cut(s) 91, 796
BmgT120I GGNCC 3 cut(s) 91, 206, 796
BmiI GGNNCC 7 cut(s) 67, 207, 911, 961, 1168, 1403, 1551
BmrFI CCNGG 2 cut(s) 494, 1194
BmrI ACTGGG 1 cut(s) 1353
BmsI GCATC 5 cut(s) 134, 428, 855, 1495, 1711
BmuI ACTGGG 1 cut(s) 1353
Bpu10I CCTNAGC 1 cut(s) 1059
BpuMI CCSGG 1 cut(s) 1194
BsaBI GATNNNNATC 1 cut(s) 1422
BsaHI GRCGYC 3 cut(s) 54, 66, 266
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 493, 963, 1173, 1192, 1515
BsaWI WCCGGW 2 cut(s) 25, 837
BsaXI ACNNNNNCTCC 6 cut(s) 22, 52, 132, 162, 279, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 284, 462
Bse118I RCCGGY 2 cut(s) 837, 912
Bse1I ACTGG 4 cut(s) 1348, 1503, 1653, 1724
Bse3DI GCAATG 1 cut(s) 394
Bse8I GATNNNNATC 1 cut(s) 1422
BseAI TCCGGA 1 cut(s) 25
BseBI CCWGG 1 cut(s) 494
BseDI CCNNGG 5 cut(s) 493, 963, 1173, 1192, 1515
BseGI GGATG 3 cut(s) 125, 445, 1353
BseJI GATNNNNATC 1 cut(s) 1422
BseLI CCNNNNNNNGG 3 cut(s) 114, 284, 462
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 5 cut(s) 528, 1050, 1603, 1607, 1750
BseNI ACTGG 4 cut(s) 1348, 1503, 1653, 1724
BseRI GAGGAG 2 cut(s) 668, 872
BseSI GKGCMC 1 cut(s) 1145
BseXI GCAGC 7 cut(s) 322, 413, 886, 1198, 1391, 1632, 1742
BseYI CCCAGC 1 cut(s) 759
BsgI GTGCAG 1 cut(s) 1761
BshFI GGCC 7 cut(s) 84, 208, 492, 521, 737, 1502, 1757
BshNI GGYRCC 3 cut(s) 65, 1166, 1549
BshTI ACCGGT 1 cut(s) 837
BsiHKAI GWGCWC 1 cut(s) 1145
BsiSI CCGG 5 cut(s) 26, 108, 838, 913, 1194
BslFI GGGAC 3 cut(s) 228, 510, 1175
BslI CCNNNNNNNGG 3 cut(s) 114, 284, 462
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 3 cut(s) 228, 510, 1175
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 7 cut(s) 84, 208, 492, 521, 737, 1502, 1757
Bso31I GGTCTC 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 1145
Bsp13I TCCGGA 1 cut(s) 25
Bsp1407I TGTACA 1 cut(s) 704
Bsp143I GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
Bsp19I CCATGG 2 cut(s) 1173, 1515
BspACI CCGC 9 cut(s) 111, 123, 152, 233, 236, 299, 322, 362, 1087
BspANI GGCC 7 cut(s) 84, 208, 492, 521, 737, 1502, 1757
BspCNI CTCAG 5 cut(s) 529, 1051, 1602, 1608, 1749
BspEI TCCGGA 1 cut(s) 25
BspLI GGNNCC 7 cut(s) 67, 207, 911, 961, 1168, 1403, 1551
BspMI ACCTGC 1 cut(s) 1652
BspPI GGATC 2 cut(s) 388, 641
BspT107I GGYRCC 3 cut(s) 65, 1166, 1549
BspTNI GGTCTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 2 cut(s) 837, 912
BsrGI TGTACA 1 cut(s) 704
BsrI ACTGG 4 cut(s) 1348, 1503, 1653, 1724
BssAI RCCGGY 2 cut(s) 837, 912
BssECI CCNNGG 5 cut(s) 493, 963, 1173, 1192, 1515
BssMI GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
BssNI GRCGYC 3 cut(s) 54, 66, 266
BssT1I CCWWGG 3 cut(s) 963, 1173, 1515
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 6 cut(s) 219, 305, 514, 724, 922, 1140
BstACI GRCGYC 3 cut(s) 54, 66, 266
BstAPI GCANNNNNTGC 1 cut(s) 1739
BstAUI TGTACA 1 cut(s) 704
BstC8I GCNNGC 3 cut(s) 360, 713, 1014
BstDEI CTNAG 8 cut(s) 398, 469, 537, 755, 1059, 1589, 1616, 1736
BstDSI CCRYGG 2 cut(s) 1173, 1515
BstF5I GGATG 3 cut(s) 125, 445, 1353
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 1702
BstKTI GATC 8 cut(s) 101, 396, 631, 649, 973, 1426, 1435, 1546
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
BstMWI GCNNNNNNNGC 6 cut(s) 233, 296, 623, 1013, 1617, 1739
BstNI CCWGG 1 cut(s) 494
BstNSI RCATGY 3 cut(s) 362, 703, 1113
BstSCI CCNGG 2 cut(s) 492, 1192
BstSLI GKGCMC 1 cut(s) 1145
BstV1I GCAGC 7 cut(s) 322, 413, 886, 1198, 1391, 1632, 1742
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
BsuRI GGCC 7 cut(s) 84, 208, 492, 521, 737, 1502, 1757
BtgI CCRYGG 2 cut(s) 1173, 1515
BtgZI GCGATG 1 cut(s) 1039
BtsCI GGATG 3 cut(s) 125, 445, 1353
BtsIMutI CAGTG 4 cut(s) 803, 826, 1145, 1744
BveI ACCTGC 1 cut(s) 1652
Cac8I GCNNGC 3 cut(s) 360, 713, 1014
CfoI GCGC 2 cut(s) 68, 1702
Cfr10I RCCGGY 2 cut(s) 837, 912
Cfr13I GGNCC 3 cut(s) 91, 206, 796
CseI GACGC 2 cut(s) 255, 324
Csp6I GTAC 6 cut(s) 691, 705, 1050, 1280, 1550, 1800
CspAI ACCGGT 1 cut(s) 837
CviAII CATG 8 cut(s) 359, 436, 700, 783, 1110, 1174, 1261, 1516
CviQI GTAC 6 cut(s) 691, 705, 1050, 1280, 1550, 1800
DdeI CTNAG 8 cut(s) 398, 469, 537, 755, 1059, 1589, 1616, 1736
DinI GGCGCC 1 cut(s) 67
DpnI GATC 8 cut(s) 100, 395, 630, 648, 972, 1425, 1434, 1545
DpnII GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
DraIII CACNNNGTG 2 cut(s) 593, 1739
EaeI YGGCCR 2 cut(s) 490, 1500
EciI GGCGGA 2 cut(s) 251, 314
Eco130I CCWWGG 3 cut(s) 963, 1173, 1515
Eco31I GGTCTC 1 cut(s) 23
Eco47I GGWCC 2 cut(s) 91, 796
Eco57I CTGAAG 2 cut(s) 912, 963
EcoRI GAATTC 1 cut(s) 777
EcoRII CCWGG 1 cut(s) 492
EcoT14I CCWWGG 3 cut(s) 963, 1173, 1515
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 3 cut(s) 963, 1173, 1515
FaeI CATG 8 cut(s) 362, 439, 703, 786, 1113, 1177, 1264, 1519
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 3 cut(s) 228, 510, 1175
FatI CATG 8 cut(s) 358, 435, 699, 782, 1109, 1173, 1260, 1515
FauNDI CATATG 2 cut(s) 251, 1120
FbaI TGATCA 1 cut(s) 970
FblI GTMKAC 3 cut(s) 60, 221, 1525
Fnu4HI GCNGC 9 cut(s) 234, 336, 427, 900, 1088, 1212, 1380, 1621, 1731
FokI GGATG 3 cut(s) 112, 452, 1360
Fsp4HI GCNGC 9 cut(s) 234, 336, 427, 900, 1088, 1212, 1380, 1621, 1731
FspBI CTAG 9 cut(s) 47, 411, 416, 423, 575, 828, 1302, 1671, 1797
FspI TGCGCA 1 cut(s) 1701
GlaI GCGC 2 cut(s) 67, 1701
GluI GCNGC 9 cut(s) 234, 336, 427, 900, 1088, 1212, 1380, 1621, 1731
GsaI CCCAGC 1 cut(s) 763
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 7 cut(s) 84, 208, 492, 521, 737, 1502, 1757
HapII CCGG 5 cut(s) 26, 108, 838, 913, 1194
HgaI GACGC 2 cut(s) 255, 324
HhaI GCGC 2 cut(s) 68, 1702
Hin1I GRCGYC 3 cut(s) 54, 66, 266
Hin1II CATG 8 cut(s) 362, 439, 703, 786, 1113, 1177, 1264, 1519
Hin6I GCGC 2 cut(s) 66, 1700
HinP1I GCGC 2 cut(s) 66, 1700
HincII GTYRAC 2 cut(s) 222, 510
HindII GTYRAC 2 cut(s) 222, 510
HindIII AAGCTT 1 cut(s) 1609
HinfI GANTC 4 cut(s) 184, 498, 950, 1335
HpaII CCGG 5 cut(s) 26, 108, 838, 913, 1194
HphI GGTGA 2 cut(s) 1147, 1504
Hpy188III TCNNGA 4 cut(s) 14, 26, 650, 1706
Hpy99I CGWCG 5 cut(s) 56, 59, 226, 232, 271
HpyAV CCTTC 3 cut(s) 139, 439, 1036
HpyCH4III ACNGT 6 cut(s) 219, 305, 514, 724, 922, 1140
HpyCH4IV ACGT 2 cut(s) 54, 194
HpyF10VI GCNNNNNNNGC 6 cut(s) 233, 296, 623, 1013, 1617, 1739
HpyF3I CTNAG 8 cut(s) 398, 469, 537, 755, 1059, 1589, 1616, 1736
HpySE526I ACGT 2 cut(s) 54, 194
Hsp92I GRCGYC 3 cut(s) 54, 66, 266
Hsp92II CATG 8 cut(s) 362, 439, 703, 786, 1113, 1177, 1264, 1519
HspAI GCGC 2 cut(s) 66, 1700
KasI GGCGCC 1 cut(s) 65
Kpn2I TCCGGA 1 cut(s) 25
KpnI GGTACC 1 cut(s) 1553
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
LmnI GCTCC 2 cut(s) 287, 1407
Lsp1109I GCAGC 7 cut(s) 322, 413, 886, 1198, 1391, 1632, 1742
LweI GCATC 5 cut(s) 134, 428, 855, 1495, 1711
MaeI CTAG 9 cut(s) 47, 411, 416, 423, 575, 828, 1302, 1671, 1797
MaeII ACGT 2 cut(s) 54, 194
MaeIII GTNAC 2 cut(s) 1664, 1722
MalI GATC 8 cut(s) 100, 395, 630, 648, 972, 1425, 1434, 1545
MboI GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
MfeI CAATTG 1 cut(s) 682
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 1 cut(s) 1145
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 5 cut(s) 682, 777, 986, 1449, 1559
MluNI TGGCCA 1 cut(s) 492
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 492
Mox20I TGGCCA 1 cut(s) 492
MroI TCCGGA 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 922
MscI TGGCCA 1 cut(s) 492
MseI TTAA 3 cut(s) 905, 1562, 1692
MslI CAYNNNNRTG 4 cut(s) 387, 1259, 1322, 1367
Msp20I TGGCCA 1 cut(s) 492
MspA1I CMGCKG 1 cut(s) 1211
MspI CCGG 5 cut(s) 26, 108, 838, 913, 1194
MspR9I CCNGG 2 cut(s) 494, 1194
MunI CAATTG 1 cut(s) 682
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 1 cut(s) 494
MwoI GCNNNNNNNGC 6 cut(s) 233, 296, 623, 1013, 1617, 1739
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 1194
NcoI CCATGG 2 cut(s) 1173, 1515
NdeI CATATG 2 cut(s) 251, 1120
NdeII GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
NlaIII CATG 8 cut(s) 362, 439, 703, 786, 1113, 1177, 1264, 1519
NlaIV GGNNCC 7 cut(s) 67, 207, 911, 961, 1168, 1403, 1551
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 1664
NsbI TGCGCA 1 cut(s) 1701
NspI RCATGY 3 cut(s) 362, 703, 1113
PaeI GCATGC 1 cut(s) 362
PctI GAATGC 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 922
PfeI GAWTC 3 cut(s) 184, 950, 1335
PflFI GACNNNGTC 2 cut(s) 53, 514
PinAI ACCGGT 1 cut(s) 837
PkrI GCNGC 9 cut(s) 235, 337, 428, 901, 1089, 1213, 1381, 1622, 1732
PleI GAGTC 1 cut(s) 492
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 492
PsiI TTATAA 1 cut(s) 1788
Psp1406I AACGTT 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 759
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 7 cut(s) 67, 207, 911, 961, 1168, 1403, 1551
PspPI GGNCC 3 cut(s) 91, 206, 796
PsuI RGATCY 1 cut(s) 646
PsyI GACNNNGTC 2 cut(s) 53, 514
PvuII CAGCTG 1 cut(s) 1211
RsaI GTAC 6 cut(s) 692, 706, 1051, 1281, 1551, 1801
RsaNI GTAC 6 cut(s) 691, 705, 1050, 1280, 1550, 1800
RseI CAYNNNNRTG 4 cut(s) 387, 1259, 1322, 1367
SalI GTCGAC 1 cut(s) 220
SaqAI TTAA 3 cut(s) 905, 1562, 1692
SatI GCNGC 9 cut(s) 234, 336, 427, 900, 1088, 1212, 1380, 1621, 1731
Sau3AI GATC 8 cut(s) 98, 393, 628, 646, 970, 1423, 1432, 1543
Sau96I GGNCC 3 cut(s) 91, 206, 796
SchI GAGTC 1 cut(s) 492
ScrFI CCNGG 2 cut(s) 494, 1194
SduI GDGCHC 1 cut(s) 1145
SfaNI GCATC 5 cut(s) 134, 428, 855, 1495, 1711
SfoI GGCGCC 1 cut(s) 67
SinI GGWCC 2 cut(s) 91, 796
SmiMI CAYNNNNRTG 4 cut(s) 387, 1259, 1322, 1367
SphI GCATGC 1 cut(s) 362
Sse9I AATT 5 cut(s) 682, 777, 986, 1449, 1559
SsiI CCGC 9 cut(s) 111, 123, 152, 233, 236, 299, 322, 362, 1087
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 1 cut(s) 549
SspMI CTAG 9 cut(s) 47, 411, 416, 423, 575, 828, 1302, 1671, 1797
StyD4I CCNGG 2 cut(s) 492, 1192
StyI CCWWGG 3 cut(s) 963, 1173, 1515
TaaI ACNGT 6 cut(s) 219, 305, 514, 724, 922, 1140
TaiI ACGT 2 cut(s) 57, 197
TaqI TCGA 4 cut(s) 15, 182, 221, 1200
TasI AATT 5 cut(s) 682, 777, 986, 1449, 1559
TatI WGTACW 2 cut(s) 704, 1799
TauI GCSGC 2 cut(s) 236, 1090
TfiI GAWTC 3 cut(s) 184, 950, 1335
Tru1I TTAA 3 cut(s) 905, 1562, 1692
Tru9I TTAA 3 cut(s) 905, 1562, 1692
TscAI CASTG 4 cut(s) 810, 826, 1145, 1744
TseFI GTSAC 1 cut(s) 1664
TseI GCWGC 7 cut(s) 335, 426, 899, 1211, 1379, 1620, 1730
Tsp45I GTSAC 1 cut(s) 1664
TspDTI ATGAA 2 cut(s) 121, 1572
TspRI CASTG 4 cut(s) 810, 826, 1145, 1744
Tth111I GACNNNGTC 2 cut(s) 53, 514
VneI GTGCAC 1 cut(s) 1141
VpaK11BI GGWCC 2 cut(s) 91, 796
XapI RAATTY 1 cut(s) 777
XceI RCATGY 3 cut(s) 362, 703, 1113
XcmI CCANNNNNNNNNTGG 1 cut(s) 1461
XmiI GTMKAC 3 cut(s) 60, 221, 1525
XmnI GAANNNNTTC 1 cut(s) 922
XspI CTAG 9 cut(s) 47, 411, 416, 423, 575, 828, 1302, 1671, 1797
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.