Rw7G032950

Protein NRT1 PTR FAMILY 5.10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000191
Physical Location & Seq
Reverse (-)
48248221 .. 48251008
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G032950.1

Sequence Viewer

Length: 1629 bp
ATGGCCACTTCTCCGGGGACCCAAACTCCGCTATTAGACGACGTCGTGGAGGGCGCCGTTGACTGCAACGGCCATCGGTCCGAAGATCCAGCTCCGGCGGTTGGCGTTGCGGAGAGGGTTGCCTACTATGGAATCAGCTCCAACCTCATATCGTTTCTGACCGGGCCTATGGGACAGTCCACGGCCACGGCGGCCAAGAACGTCAACATATGGTCCGGAACGGCGTCGTTGCTTCCTCTATTGGGAGCTTTCGTTGCTGATTCTTTCCTGGGTCGCTACCGCACCATTGTCGCCGCTTCTCTCCTCTACATCATGGGTCTTGGGTTGCTCACTGTGTCAGCCGTGCTTCCTTCTTCTCAGCTTCAAGTATTATTATTCTTTTTTTCTCTATATTTAGTAGCAGTTGGGCAAGGTGGACACAAGCCTTGTGTTCAGGCTTTCGGAGCAGATCAGTTTGATAGACAAGATCCAGAGGAGTGCAAAGCAAAAAGCTCATTCTTCAATTGGTGGTATTTTGGTATATGTGCAGGCCCTTTATTGACACTTACAGTATTAGACTACATACAGGACAATCTTAGTTGGGGTCTGGGTTTTGGTCTTCCTTGCATTGTGATGGCCTTTGCACTGCTTATTTTCCTTCTTGGAACAAGAACGTACCGGTATAGTATCAAAGGGGATGAGGAAAGCCCATTTGTGAGAATTGGCAAGGTGTTTGCTGCTGCATTAAGGAATTGGCGAACTACTCCTTCAGCAATAGCTTCAGAAGAGGAATCTCGTGGAACCTTGCCTCACCAAAGTTCTGAACAATTCAAATTCCTTAACAAAGCCTTGGTTGCACCGGATGATTTGAAGGAAAATGGGAAGGTCTGTCCCATCACTGAGGTTGAAGAAGCAAAAGCTGTTCTTAGGTTGTTTCCGATATGGGTTACATGCTTGGTATATGCTATTGTGTTTGCGCAGTTCTCGACTTTCTTCACTAAGCAAGGTGCCACCATGGACCGAACATTTGTGCCAGGTTTCGACATACCAGCTGCTGCACTTCAGTCATTTATAAGCCTTGTCATTATTATATTCATTCCCATTTATGATCGTATATTTGTCCCAATAGCAAGATCATTCACCAGGGAACCCTCTGGCATTACTATGCTACAAAGAATAGGAACTGGGATGGTTATATCTACTATTTCCATGGTAGTTGCAGCTTTAGTTGAGATACAGAGGCTCCAAACTGCCAAAGATTATGATCTGGTTGATATGCCAAGTGCCACTGTTCCGATGAGTGTTTGGTGGTTAGTTCCTCAATATTTGTTAACCGGACTGGCAGATGTTTTCACCATGGTTGGTCTGCAAGAGTTCTTCTATGATCAGATGCCAAAAGAATTAAGAAGTGTAGGACTTGCCCTCTACCTCAGTATATTTGGTGTCGGAAGCTTCCTGAGCAGCTTTCTTATCTCTGTTATTGAGGAAGGAACCAGTTGGGCAGGCCACACTAGCTGGTTTTCCAATAACCTTAACCGTGCACATCTTGATTACTTTTATTGGTTACTAGCTGTACTCAGTGCAGTACAATTGGCCATCTACATGTGTATAGCAAAATCTTACATTTATAACAGGGGTGGTACAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

542

Amino Acids

59.58

Weight (kDa)

5.4

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 46 - 480 2.3e-15 Major Facilitator Superfamily
PTR2 PF00854 93 - 499 1.4e-98 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22540
fragaria_vesca FvH4_5g31770 FvH4_5g31770 FvH4_5g31790 FvH4_5g31790 FvH4_5g31800 FvH4_5g31800 FvH4_5g31800 FvH4_5g31830 FvH4_5g31840 FvH4_5g31840 FvH4_6g12331
malus_domestica MD02G1288000.v1.1 MD02G1288200.v1.1 MD03G1076700.v1.1 MD07G1122800.v1.1 MD08G1218300.v1.1 MD15G1406500.v1.1 MD15G1406600.v1.1 MD15G1406700.v1.1
prunus_persica Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1
pyrus_communis pycom05g17900 pycom08g18920 pycom15g36290 pycom15g36300 pycom15g36310
rosa_chinensis RchiOBHm_Chr3g0482421 RchiOBHm_Chr4g0413471 RchiOBHm_Chr7g0227651 RchiOBHm_Chr7g0227661 RchiOBHm_Chr7g0227671 RchiOBHm_Chr7g0227681 RchiOBHm_Chr7g0227701 RchiOBHm_Chr7g0227711 RchiOBHm_Chr7g0227721 RchiOBHm_Chr7g0227731 RchiOBHm_Chr7g0227741 RchiOBHm_Chr7g0227761 RchiOBHm_Chr7g0227781 RchiOBHm_Chr7g0227791 RchiOBHm_Chr7g0227801 RchiOBHm_Chr7g0227861 RchiOBHm_Chr7g0227881
rosa_laevigata RLG00000001610 RLG00000001611 RLG00000001613 RLG00000001617 RLG00000001618 RLG00000001619 RLG00000001622 RLG00000001623 RLG00000001625 RLG00000001627 RLG00000001628 RLG00000001629 RLG00000001630 RLG00000008217
rosa_multiflora Rmu_co8446845.1_g000001 Rmu_co8488273.1_g000001 Rmu_sc0000347.1_g000023 Rmu_sc0000347.1_g000032 Rmu_sc0000347.1_g000038 Rmu_sc0000347.1_g000046 Rmu_sc0000347.1_g000047 Rmu_sc0000347.1_g000049 Rmu_sc0000347.1_g000050 Rmu_sc0000883.1_g000014 Rmu_sc0000883.1_g000015 Rmu_sc0001930.1_g000001 Rmu_sc0001930.1_g000006 Rmu_sc0005026.1_g000009 Rmu_sc0005026.1_g000013 Rmu_sc0005026.1_g000018 Rmu_sc0005026.1_g000021 Rmu_sc0005743.1_g000008 Rmu_sc0005743.1_g000010 Rmu_sc0010635.1_g000001 Rmu_sc0013384.1_g000001 Rmu_sc0013521.1_g000001 Rmu_sc0014985.1_g000003 Rmu_sc0022490.1_g000001 Rmu_sc0025092.1_g000001 Rmu_sc0036408.1_g000001
rosa_roxburghii Rroxscaffold_3G00231820 Rroxscaffold_3G00231860 Rroxscaffold_3G00231890 Rroxscaffold_3G00231910 Rroxscaffold_3G00231930 Rroxscaffold_3G00231940 Rroxscaffold_3G00231960 Rroxscaffold_3G00231970 Rroxscaffold_3G00231980 Rroxscaffold_5G00357370
rosa_rugosa Rorug03G0196300 Rorug04G0120600 Rorug07G0242700 Rorug07G0242800 Rorug07G0242900 Rorug07G0243000 Rorug07G0243100 Rorug07G0243200 Rorug07G0243400 Rorug07G0243600 Rorug07G0243800 Rorug07G0243900
rosa_samantha Rh3AG109500 Rh3AG248000 Rh3BG283000 Rh3CG114700 Rh3CG279600 Rh3DG275400 Rh3DG275500 Rh3DG275800 Rh4AG180200 Rh4BG179400 Rh4DG175400 Rh6AG297600 Rh6CG311000 Rh7AG396200 Rh7AG396300 Rh7AG396400 Rh7AG396600 Rh7AG397000 Rh7AG397100 Rh7BG377800 Rh7BG377900 Rh7BG378000 Rh7BG378100 Rh7BG378300 Rh7BG378400 Rh7BG378500 Rh7BG378800 Rh7BG378900 Rh7DG391900 Rh7DG392000 Rh7DG392300 Rh7DG392400 Rh7DG392700
rosa_wichuraiana Rw3G022320 Rw4G015000 Rw4G015060 Rw7G032940 Rw7G032950 Rw7G032960 Rw7G032970 Rw7G032980 Rw7G032990 Rw7G033000 Rw7G033010 Rw7G033020 Rw7G033030 Rw7G033050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1052, 1608
AatII GACGTC 1 cut(s) 45
Acc16I TGCGCA 1 cut(s) 957
AccB1I GGYRCC 2 cut(s) 53, 986
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 6 cut(s) 29, 98, 110, 191, 280, 294
AclWI GGATC 2 cut(s) 80, 461
AcoI YGGCCR 5 cut(s) 3, 70, 183, 192, 1572
AcsI RAATTY 1 cut(s) 812
AcuI CTGAAG 3 cut(s) 732, 744, 1025
AcyI GRCGYC 3 cut(s) 42, 54, 224
AfaI GTAC 4 cut(s) 656, 1554, 1566, 1621
AfiI CCNNNNNNNGG 2 cut(s) 101, 242
AflIII ACRYGT 1 cut(s) 1581
AgeI ACCGGT 1 cut(s) 657
AgsI TTSAA 5 cut(s) 365, 502, 811, 850, 887
AjnI CCWGG 3 cut(s) 267, 1012, 1121
Alw21I GWGCWC 1 cut(s) 1522
Alw44I GTGCAC 1 cut(s) 1518
AlwI GGATC 2 cut(s) 80, 461
AlwNI CAGNNNCTG 1 cut(s) 1034
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 9 cut(s) 3, 70, 164, 183, 192, 529, 615, 1483, 1572
ApaLI GTGCAC 1 cut(s) 1518
ApeKI GCWGC 6 cut(s) 716, 719, 1031, 1034, 1199, 1440
ApoI RAATTY 1 cut(s) 812
ArsI GACNNNNNNTTYG 2 cut(s) 989, 1021
AsiGI ACCGGT 1 cut(s) 657
AspLEI GCGC 2 cut(s) 56, 958
AspS9I GGNCC 6 cut(s) 18, 78, 164, 213, 530, 997
AsuC2I CCSGG 2 cut(s) 15, 163
AsuHPI GGTGA 3 cut(s) 782, 1111, 1324
AvaII GGWCC 4 cut(s) 18, 78, 213, 997
BaeGI GKGCMC 1 cut(s) 1522
BalI TGGCCA 2 cut(s) 5, 1574
BanI GGYRCC 2 cut(s) 53, 986
BauI CACGAG 1 cut(s) 774
BbsI GAAGAC 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 1522
BbvI GCAGC 6 cut(s) 703, 706, 1018, 1021, 1211, 1452
BccI CCATC 5 cut(s) 81, 607, 881, 1162, 1583
BceAI ACGGC 6 cut(s) 41, 85, 198, 204, 237, 326
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 3 cut(s) 269, 1014, 1123
BclI TGATCA 1 cut(s) 1363
BcnI CCSGG 2 cut(s) 15, 163
BfaI CTAG 2 cut(s) 1491, 1547
BfoI RGCGCY 1 cut(s) 57
BglI GCCNNNNNGGC 1 cut(s) 191
BisI GCNGC 8 cut(s) 192, 294, 717, 720, 1032, 1035, 1200, 1441
BlsI GCNGC 8 cut(s) 193, 295, 718, 721, 1033, 1036, 1201, 1442
Bme1390I CCNGG 5 cut(s) 15, 163, 269, 1014, 1123
Bme18I GGWCC 4 cut(s) 18, 78, 213, 997
BmgT120I GGNCC 6 cut(s) 18, 78, 164, 213, 530, 997
BmiI GGNNCC 8 cut(s) 19, 20, 55, 781, 988, 1128, 1223, 1471
BmrFI CCNGG 5 cut(s) 15, 163, 269, 1014, 1123
BmrI ACTGGG 1 cut(s) 1173
BmsI GCATC 1 cut(s) 1359
BmuI ACTGGG 1 cut(s) 1173
BpiI GAAGAC 1 cut(s) 590
Bpu10I CCTNAGC 1 cut(s) 1436
BpuMI CCSGG 2 cut(s) 15, 163
BsaBI GATNNNNATC 1 cut(s) 1242
BsaHI GRCGYC 3 cut(s) 42, 54, 224
BsaJI CCNNGG 9 cut(s) 14, 180, 186, 268, 828, 993, 1122, 1188, 1335
BsaWI WCCGGW 4 cut(s) 215, 657, 838, 1313
BsaXI ACNNNNNCTCC 6 cut(s) 10, 40, 237, 267, 1206, 1236
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 242
Bse118I RCCGGY 1 cut(s) 657
Bse1I ACTGG 3 cut(s) 1168, 1323, 1473
Bse8I GATNNNNATC 1 cut(s) 1242
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 3 cut(s) 269, 1014, 1123
BseDI CCNNGG 9 cut(s) 14, 180, 186, 268, 828, 993, 1122, 1188, 1335
BseGI GGATG 3 cut(s) 682, 847, 1173
BseJI GATNNNNATC 1 cut(s) 1242
BseLI CCNNNNNNNGG 2 cut(s) 101, 242
BseMII CTCAG 5 cut(s) 371, 870, 1423, 1427, 1570
BseNI ACTGG 3 cut(s) 1168, 1323, 1473
BseRI GAGGAG 2 cut(s) 293, 488
BseSI GKGCMC 1 cut(s) 1522
BseXI GCAGC 6 cut(s) 703, 706, 1018, 1021, 1211, 1452
BsgI GTGCAG 3 cut(s) 546, 1020, 1581
BshFI GGCC 9 cut(s) 5, 72, 166, 185, 194, 531, 617, 1485, 1574
BshNI GGYRCC 2 cut(s) 53, 986
BshTI ACCGGT 1 cut(s) 657
BsiHKAI GWGCWC 1 cut(s) 1522
BsiSI CCGG 7 cut(s) 14, 95, 162, 216, 658, 839, 1314
BslFI GGGAC 4 cut(s) 31, 186, 855, 1085
BslI CCNNNNNNNGG 2 cut(s) 101, 242
BsmFI GGGAC 4 cut(s) 31, 186, 855, 1085
BsnI GGCC 9 cut(s) 5, 72, 166, 185, 194, 531, 617, 1485, 1574
Bsp1286I GDGCHC 1 cut(s) 1522
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
Bsp19I CCATGG 3 cut(s) 993, 1188, 1335
BspACI CCGC 6 cut(s) 29, 98, 110, 191, 280, 294
BspANI GGCC 9 cut(s) 5, 72, 166, 185, 194, 531, 617, 1485, 1574
BspCNI CTCAG 5 cut(s) 370, 871, 1422, 1428, 1569
BspEI TCCGGA 1 cut(s) 215
BspLI GGNNCC 8 cut(s) 19, 20, 55, 781, 988, 1128, 1223, 1471
BspPI GGATC 2 cut(s) 80, 461
BspT107I GGYRCC 2 cut(s) 53, 986
BsrFI RCCGGY 1 cut(s) 657
BsrI ACTGG 3 cut(s) 1168, 1323, 1473
BssAI RCCGGY 1 cut(s) 657
BssECI CCNNGG 9 cut(s) 14, 180, 186, 268, 828, 993, 1122, 1188, 1335
BssMI GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
BssNI GRCGYC 3 cut(s) 42, 54, 224
BssSI CACGAG 1 cut(s) 774
BssT1I CCWWGG 4 cut(s) 828, 993, 1188, 1335
Bst2BI CACGAG 1 cut(s) 774
Bst2UI CCWGG 3 cut(s) 269, 1014, 1123
Bst4CI ACNGT 6 cut(s) 177, 334, 550, 1270, 1517, 1624
Bst6I CTCTTC 1 cut(s) 759
BstACI GRCGYC 3 cut(s) 42, 54, 224
BstC8I GCNNGC 2 cut(s) 529, 1483
BstDEI CTNAG 8 cut(s) 357, 575, 879, 905, 978, 1409, 1436, 1556
BstDSI CCRYGG 5 cut(s) 180, 186, 993, 1188, 1335
BstF5I GGATG 3 cut(s) 682, 847, 1173
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 2 cut(s) 56, 958
BstKTI GATC 7 cut(s) 88, 451, 469, 1090, 1115, 1246, 1366
BstMBI GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
BstMWI GCNNNNNNNGC 6 cut(s) 191, 254, 443, 833, 1437, 1491
BstNI CCWGG 3 cut(s) 269, 1014, 1123
BstNSI RCATGY 2 cut(s) 933, 1585
BstSCI CCNGG 5 cut(s) 13, 161, 267, 1012, 1121
BstSLI GKGCMC 1 cut(s) 1522
BstV1I GCAGC 6 cut(s) 703, 706, 1018, 1021, 1211, 1452
BstV2I GAAGAC 1 cut(s) 590
BstX2I RGATCY 2 cut(s) 85, 466
BstYI RGATCY 2 cut(s) 85, 466
BsuRI GGCC 9 cut(s) 5, 72, 166, 185, 194, 531, 617, 1485, 1574
BtgI CCRYGG 5 cut(s) 180, 186, 993, 1188, 1335
BtsCI GGATG 3 cut(s) 682, 847, 1173
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 5 cut(s) 330, 623, 876, 1266, 1564
Cac8I GCNNGC 2 cut(s) 529, 1483
CaiI CAGNNNCTG 1 cut(s) 1034
CfoI GCGC 2 cut(s) 56, 958
Cfr10I RCCGGY 1 cut(s) 657
Cfr13I GGNCC 6 cut(s) 18, 78, 164, 213, 530, 997
CpoI CGGWCCG 1 cut(s) 78
CseI GACGC 1 cut(s) 213
Csp6I GTAC 4 cut(s) 655, 1553, 1565, 1620
CspAI ACCGGT 1 cut(s) 657
CspI CGGWCCG 1 cut(s) 78
CviAII CATG 6 cut(s) 313, 930, 994, 1189, 1336, 1582
CviQI GTAC 4 cut(s) 655, 1553, 1565, 1620
DdeI CTNAG 8 cut(s) 357, 575, 879, 905, 978, 1409, 1436, 1556
DinI GGCGCC 1 cut(s) 55
DpnI GATC 7 cut(s) 87, 450, 468, 1089, 1114, 1245, 1365
DpnII GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
EaeI YGGCCR 5 cut(s) 3, 70, 183, 192, 1572
Eam1104I CTCTTC 1 cut(s) 759
EarI CTCTTC 1 cut(s) 759
Eco130I CCWWGG 4 cut(s) 828, 993, 1188, 1335
Eco47I GGWCC 4 cut(s) 18, 78, 213, 997
Eco57I CTGAAG 3 cut(s) 732, 744, 1025
EcoO109I RGGNCCY 2 cut(s) 18, 530
EcoRII CCWGG 3 cut(s) 267, 1012, 1121
EcoT14I CCWWGG 4 cut(s) 828, 993, 1188, 1335
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 4 cut(s) 828, 993, 1188, 1335
FaeI CATG 6 cut(s) 316, 933, 997, 1192, 1339, 1585
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 4 cut(s) 31, 186, 855, 1085
FatI CATG 6 cut(s) 312, 929, 993, 1188, 1335, 1581
FauNDI CATATG 1 cut(s) 209
FbaI TGATCA 1 cut(s) 1363
Fnu4HI GCNGC 8 cut(s) 192, 294, 717, 720, 1032, 1035, 1200, 1441
FokI GGATG 3 cut(s) 689, 854, 1180
Fsp4HI GCNGC 8 cut(s) 192, 294, 717, 720, 1032, 1035, 1200, 1441
FspBI CTAG 2 cut(s) 1491, 1547
FspI TGCGCA 1 cut(s) 957
GlaI GCGC 2 cut(s) 55, 957
GluI GCNGC 8 cut(s) 192, 294, 717, 720, 1032, 1035, 1200, 1441
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 9 cut(s) 5, 72, 166, 185, 194, 531, 617, 1485, 1574
HapII CCGG 7 cut(s) 14, 95, 162, 216, 658, 839, 1314
HgaI GACGC 1 cut(s) 213
HhaI GCGC 2 cut(s) 56, 958
Hin1I GRCGYC 3 cut(s) 42, 54, 224
Hin1II CATG 6 cut(s) 316, 933, 997, 1192, 1339, 1585
Hin6I GCGC 2 cut(s) 54, 956
HinP1I GCGC 2 cut(s) 54, 956
HincII GTYRAC 3 cut(s) 61, 205, 1311
HindII GTYRAC 3 cut(s) 61, 205, 1311
HindIII AAGCTT 1 cut(s) 1429
HinfI GANTC 3 cut(s) 132, 260, 770
HpaI GTTAAC 1 cut(s) 1311
HpaII CCGG 7 cut(s) 14, 95, 162, 216, 658, 839, 1314
HphI GGTGA 3 cut(s) 782, 1111, 1324
Hpy166II GTNNAC 6 cut(s) 61, 180, 205, 416, 1311, 1520
Hpy188I TCNGA 9 cut(s) 82, 159, 443, 763, 802, 918, 1275, 1368, 1427
Hpy188III TCNNGA 5 cut(s) 216, 470, 964, 1435, 1526
Hpy8I GTNNAC 6 cut(s) 61, 180, 205, 416, 1311, 1520
Hpy99I CGWCG 3 cut(s) 44, 47, 229
HpyAV CCTTC 6 cut(s) 360, 647, 756, 844, 856, 1460
HpyCH4III ACNGT 6 cut(s) 177, 334, 550, 1270, 1517, 1624
HpyCH4IV ACGT 3 cut(s) 42, 201, 653
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 254, 443, 833, 1437, 1491
HpyF3I CTNAG 8 cut(s) 357, 575, 879, 905, 978, 1409, 1436, 1556
HpySE526I ACGT 3 cut(s) 42, 201, 653
Hsp92I GRCGYC 3 cut(s) 42, 54, 224
Hsp92II CATG 6 cut(s) 316, 933, 997, 1192, 1339, 1585
HspAI GCGC 2 cut(s) 54, 956
KasI GGCGCC 1 cut(s) 53
KflI GGGWCCC 1 cut(s) 18
Kpn2I TCCGGA 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 1363
KspAI GTTAAC 1 cut(s) 1311
Kzo9I GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
LmnI GCTCC 5 cut(s) 97, 143, 245, 443, 1227
Lsp1109I GCAGC 6 cut(s) 703, 706, 1018, 1021, 1211, 1452
LweI GCATC 1 cut(s) 1359
MaeI CTAG 2 cut(s) 1491, 1547
MaeII ACGT 3 cut(s) 42, 201, 653
MaeIII GTNAC 2 cut(s) 925, 1542
MalI GATC 7 cut(s) 87, 450, 468, 1089, 1114, 1245, 1365
MboI GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
MboII GAAGA 8 cut(s) 95, 345, 490, 590, 776, 899, 964, 1348
MfeI CAATTG 2 cut(s) 502, 1568
MflI RGATCY 2 cut(s) 85, 466
MhlI GDGCHC 1 cut(s) 1522
MlsI TGGCCA 2 cut(s) 5, 1574
MluCI AATT 7 cut(s) 502, 699, 730, 806, 812, 1379, 1568
MluNI TGGCCA 2 cut(s) 5, 1574
Mly113I GGCGCC 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 165, 1405
Mox20I TGGCCA 2 cut(s) 5, 1574
MroI TCCGGA 1 cut(s) 215
MscI TGGCCA 2 cut(s) 5, 1574
MseI TTAA 5 cut(s) 725, 819, 1310, 1382, 1512
MslI CAYNNNNRTG 3 cut(s) 611, 1142, 1580
Msp20I TGGCCA 2 cut(s) 5, 1574
MspA1I CMGCKG 1 cut(s) 1031
MspI CCGG 7 cut(s) 14, 95, 162, 216, 658, 839, 1314
MspR9I CCNGG 5 cut(s) 15, 163, 269, 1014, 1123
MunI CAATTG 2 cut(s) 502, 1568
MvaI CCWGG 3 cut(s) 269, 1014, 1123
MwoI GCNNNNNNNGC 6 cut(s) 191, 254, 443, 833, 1437, 1491
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 2 cut(s) 15, 163
NcoI CCATGG 3 cut(s) 993, 1188, 1335
NdeI CATATG 1 cut(s) 209
NdeII GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
NlaIII CATG 6 cut(s) 316, 933, 997, 1192, 1339, 1585
NlaIV GGNNCC 8 cut(s) 19, 20, 55, 781, 988, 1128, 1223, 1471
NsbI TGCGCA 1 cut(s) 957
NspI RCATGY 2 cut(s) 933, 1585
PciI ACATGT 1 cut(s) 1581
PfeI GAWTC 3 cut(s) 132, 260, 770
PflFI GACNNNGTC 1 cut(s) 41
PinAI ACCGGT 1 cut(s) 657
PkrI GCNGC 8 cut(s) 193, 295, 718, 721, 1033, 1036, 1201, 1442
PluTI GGCGCC 1 cut(s) 57
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 1581
PsiI TTATAA 2 cut(s) 1052, 1608
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 267, 1012, 1121
PspGI CCWGG 3 cut(s) 267, 1012, 1121
PspN4I GGNNCC 8 cut(s) 19, 20, 55, 781, 988, 1128, 1223, 1471
PspPI GGNCC 6 cut(s) 18, 78, 164, 213, 530, 997
PspPPI RGGWCCY 1 cut(s) 18
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 2 cut(s) 85, 466
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 4 cut(s) 656, 1554, 1566, 1621
RsaNI GTAC 4 cut(s) 655, 1553, 1565, 1620
RseI CAYNNNNRTG 3 cut(s) 611, 1142, 1580
Rsr2I CGGWCCG 1 cut(s) 78
RsrII CGGWCCG 1 cut(s) 78
SaqAI TTAA 5 cut(s) 725, 819, 1310, 1382, 1512
SatI GCNGC 8 cut(s) 192, 294, 717, 720, 1032, 1035, 1200, 1441
Sau3AI GATC 7 cut(s) 85, 448, 466, 1087, 1112, 1243, 1363
Sau96I GGNCC 6 cut(s) 18, 78, 164, 213, 530, 997
ScrFI CCNGG 5 cut(s) 15, 163, 269, 1014, 1123
SduI GDGCHC 1 cut(s) 1522
SfaNI GCATC 1 cut(s) 1359
SfiI GGCCNNNNNGGCC 1 cut(s) 191
SfoI GGCGCC 1 cut(s) 55
SinI GGWCC 4 cut(s) 18, 78, 213, 997
SmiMI CAYNNNNRTG 3 cut(s) 611, 1142, 1580
Sse9I AATT 7 cut(s) 502, 699, 730, 806, 812, 1379, 1568
SsiI CCGC 6 cut(s) 29, 98, 110, 191, 280, 294
SspDI GGCGCC 1 cut(s) 53
SspI AATATT 1 cut(s) 1304
SspMI CTAG 2 cut(s) 1491, 1547
StyD4I CCNGG 5 cut(s) 13, 161, 267, 1012, 1121
StyI CCWWGG 4 cut(s) 828, 993, 1188, 1335
TaaI ACNGT 6 cut(s) 177, 334, 550, 1270, 1517, 1624
TaiI ACGT 3 cut(s) 45, 204, 656
TaqI TCGA 2 cut(s) 965, 1020
TaqII GACCGA 2 cut(s) 66, 1014
TasI AATT 7 cut(s) 502, 699, 730, 806, 812, 1379, 1568
TatI WGTACW 2 cut(s) 1552, 1564
TauI GCSGC 2 cut(s) 194, 296
TfiI GAWTC 3 cut(s) 132, 260, 770
Tru1I TTAA 5 cut(s) 725, 819, 1310, 1382, 1512
Tru9I TTAA 5 cut(s) 725, 819, 1310, 1382, 1512
TscAI CASTG 5 cut(s) 337, 630, 883, 1273, 1564
TseI GCWGC 6 cut(s) 716, 719, 1031, 1034, 1199, 1440
TspDTI ATGAA 1 cut(s) 1063
TspRI CASTG 5 cut(s) 337, 630, 883, 1273, 1564
Tth111I GACNNNGTC 1 cut(s) 41
VneI GTGCAC 1 cut(s) 1518
VpaK11BI GGWCC 4 cut(s) 18, 78, 213, 997
XapI RAATTY 1 cut(s) 812
XceI RCATGY 2 cut(s) 933, 1585
XspI CTAG 2 cut(s) 1491, 1547
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.