Rh7BG378400

Protein NRT1 PTR FAMILY 5.10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000191
Physical Location & Seq
Reverse (-)
40697592 .. 40700895
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG378400.1

Sequence Viewer

Length: 1287 bp
ATGCTTACTTCTTCTGAGCTTCAAATAGTGTTATTCTTCTTCTCTCTATATCTAGTAGCAGTTGCGCAAGGTGGACACAAGCCCTGTGTTCAGGCTTTCGGCGCAGATCAGTTTGATGGACAAGATCCAGAGGAGTGCAAAGCGAAAAGCTCATTCTTCAATTGGTGGTACTTTGGTATGAATGCAGGCATACTATTGACATTCTCGGTATTGATCTACATACAGGACAATCTAAGCTGGGGTCTGGGTTTTGGAATTCCTTGTGTTGTGATGGTCCTTGGAATGATTATTTTTGTCAGTGGAACTAGGACATACCGGTATAGCATCCAAGGGGAGGAGGAAAGTCCATTTGTGAGAATTGGCAAGGTTTTTGTTGCTGCATTAAGGAACCGGCGAACTAGTCCTTCAGCAATAGCTTCTGAAGAGGAATCCCGTGGAACCTTGCCTGATCAAAGGTCTGAACAATTCAAGTTCCTCAACAAAGCCTTGCTTGCACCAGATAATTTGAAGAAAAACGGGAAGGTGTGTACCATCGCTGAGGTTGAGGAAGCAAAGGCTGTTCTGAGGCTGTTTCCAATATGGGCTACATGCTTGCCATATGCTATTGTATGTGCACAGTCCAGTACTTTCTTCACCAAGCAAGGTGCTACCATGGACAGAACTATTGTGCCCGGTTTTGACATACCAGCTGCTTCACTTCAGTCATTTATCTGCCTTGCCATAATGATGATCATACCCATTTATGACCGCATTTTTGTTCCAATGGCAAGAGCTTTCACCAGAGAACCCTTTGGCATTACAAAGCTACAAAGAATCGGAACTGGGATGTTTTTCTCTATCATATCTATGGTCGTTGCAGCTTTAGTTGAGATGAAAAGGCTCCAAACTGCCAAAGATTATGATCTGGTTGATCTGCCAAGTGCCACAATTCCAATGAGTATTTGGTGGTTGGTTCCTCAGTACTTTTTGTATGGACTAGCCGATGTTTTCACCATTGTTGGTCTACAAGAGTTCTTCTATGATCAGGTACCAAATGAATTAAGAAGCATGGGACTTGCCCTCTACCTCAGTATATTTGGTGTGGGAAACTTTCTGAGCAGCTTTCTTATCTCTATTATTGAGGAAGCAACCAGTTGGGGAGGCCACACTAGCTGGTTTTCCGACAACCTTAATCTTGCACATCTTGATTACTTTTACTGGTTACTAGCAGCACTCTGTGGAGTAGGATTGTTGGCCTACATGTATTTTGCAAAATCTTACATTTACAATAGGGCTAGTACAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

428

Amino Acids

48.02

Weight (kDa)

5.76

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 6 - 385 1.6e-91 POT family
MFS_1 PF07690 11 - 366 2.4e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22540
fragaria_vesca FvH4_5g31770 FvH4_5g31770 FvH4_5g31790 FvH4_5g31790 FvH4_5g31800 FvH4_5g31800 FvH4_5g31800 FvH4_5g31830 FvH4_5g31840 FvH4_5g31840 FvH4_6g12331
malus_domestica MD02G1288000.v1.1 MD02G1288200.v1.1 MD03G1076700.v1.1 MD07G1122800.v1.1 MD08G1218300.v1.1 MD15G1406500.v1.1 MD15G1406600.v1.1 MD15G1406700.v1.1
prunus_persica Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1 Prupe.7G017300_v2.0.a1
pyrus_communis pycom05g17900 pycom08g18920 pycom15g36290 pycom15g36300 pycom15g36310
rosa_chinensis RchiOBHm_Chr3g0482421 RchiOBHm_Chr4g0413471 RchiOBHm_Chr7g0227651 RchiOBHm_Chr7g0227661 RchiOBHm_Chr7g0227671 RchiOBHm_Chr7g0227681 RchiOBHm_Chr7g0227701 RchiOBHm_Chr7g0227711 RchiOBHm_Chr7g0227721 RchiOBHm_Chr7g0227731 RchiOBHm_Chr7g0227741 RchiOBHm_Chr7g0227761 RchiOBHm_Chr7g0227781 RchiOBHm_Chr7g0227791 RchiOBHm_Chr7g0227801 RchiOBHm_Chr7g0227861 RchiOBHm_Chr7g0227881
rosa_laevigata RLG00000001610 RLG00000001611 RLG00000001613 RLG00000001617 RLG00000001618 RLG00000001619 RLG00000001622 RLG00000001623 RLG00000001625 RLG00000001627 RLG00000001628 RLG00000001629 RLG00000001630 RLG00000008217
rosa_multiflora Rmu_co8446845.1_g000001 Rmu_co8488273.1_g000001 Rmu_sc0000347.1_g000023 Rmu_sc0000347.1_g000032 Rmu_sc0000347.1_g000038 Rmu_sc0000347.1_g000046 Rmu_sc0000347.1_g000047 Rmu_sc0000347.1_g000049 Rmu_sc0000347.1_g000050 Rmu_sc0000883.1_g000014 Rmu_sc0000883.1_g000015 Rmu_sc0001930.1_g000001 Rmu_sc0001930.1_g000006 Rmu_sc0005026.1_g000009 Rmu_sc0005026.1_g000013 Rmu_sc0005026.1_g000018 Rmu_sc0005026.1_g000021 Rmu_sc0005743.1_g000008 Rmu_sc0005743.1_g000010 Rmu_sc0010635.1_g000001 Rmu_sc0013384.1_g000001 Rmu_sc0013521.1_g000001 Rmu_sc0014985.1_g000003 Rmu_sc0022490.1_g000001 Rmu_sc0025092.1_g000001 Rmu_sc0036408.1_g000001
rosa_roxburghii Rroxscaffold_3G00231820 Rroxscaffold_3G00231860 Rroxscaffold_3G00231890 Rroxscaffold_3G00231910 Rroxscaffold_3G00231930 Rroxscaffold_3G00231940 Rroxscaffold_3G00231960 Rroxscaffold_3G00231970 Rroxscaffold_3G00231980 Rroxscaffold_5G00357370
rosa_rugosa Rorug03G0196300 Rorug04G0120600 Rorug07G0242700 Rorug07G0242800 Rorug07G0242900 Rorug07G0243000 Rorug07G0243100 Rorug07G0243200 Rorug07G0243400 Rorug07G0243600 Rorug07G0243800 Rorug07G0243900
rosa_samantha Rh3AG109500 Rh3AG248000 Rh3BG283000 Rh3CG114700 Rh3CG279600 Rh3DG275400 Rh3DG275500 Rh3DG275800 Rh4AG180200 Rh4BG179400 Rh4DG175400 Rh6AG297600 Rh6CG311000 Rh7AG396200 Rh7AG396300 Rh7AG396400 Rh7AG396600 Rh7AG397000 Rh7AG397100 Rh7BG377800 Rh7BG377900 Rh7BG378000 Rh7BG378100 Rh7BG378300 Rh7BG378400 Rh7BG378500 Rh7BG378800 Rh7BG378900 Rh7DG391900 Rh7DG392000 Rh7DG392300 Rh7DG392400 Rh7DG392700
rosa_wichuraiana Rw3G022320 Rw4G015000 Rw4G015060 Rw7G032940 Rw7G032950 Rw7G032960 Rw7G032970 Rw7G032980 Rw7G032990 Rw7G033000 Rw7G033010 Rw7G033020 Rw7G033030 Rw7G033050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 66
Acc65I GGTACC 1 cut(s) 1027
AccB1I GGYRCC 1 cut(s) 1027
AccI GTMKAC 1 cut(s) 1003
AciI CCGC 1 cut(s) 748
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 3 cut(s) 390, 441, 683
AdeI CACNNNGTG 1 cut(s) 1217
AfaI GTAC 6 cut(s) 170, 529, 625, 962, 1029, 1279
AfiI CCNNNNNNNGG 1 cut(s) 334
AflIII ACRYGT 1 cut(s) 1239
AgeI ACCGGT 1 cut(s) 315
AgsI TTSAA 4 cut(s) 23, 160, 469, 508
AhlI ACTAGT 1 cut(s) 398
Alw21I GWGCWC 1 cut(s) 616
Alw44I GTGCAC 1 cut(s) 612
AlwI GGATC 1 cut(s) 119
AoxI GGCC 2 cut(s) 1141, 1233
ApaLI GTGCAC 1 cut(s) 612
ApeKI GCWGC 5 cut(s) 377, 689, 857, 1098, 1208
ApoI RAATTY 1 cut(s) 255
ArsI GACNNNNNNTTYG 2 cut(s) 737, 769
AsiGI ACCGGT 1 cut(s) 315
Asp718I GGTACC 1 cut(s) 1027
AspLEI GCGC 2 cut(s) 67, 104
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 3 cut(s) 625, 769, 982
AvaII GGWCC 1 cut(s) 274
BaeGI GKGCMC 2 cut(s) 616, 672
BaeI ACNNNNGTAYC 2 cut(s) 160, 193
BanI GGYRCC 1 cut(s) 1027
Bbv12I GWGCWC 1 cut(s) 616
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 5 cut(s) 364, 676, 869, 1110, 1220
BccI CCATC 3 cut(s) 110, 265, 539
BclI TGATCA 3 cut(s) 448, 729, 1021
BcnI CCSGG 1 cut(s) 672
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 7 cut(s) 53, 306, 399, 977, 1149, 1205, 1275
BisI GCNGC 5 cut(s) 378, 690, 858, 1099, 1209
BlsI GCNGC 5 cut(s) 379, 691, 859, 1100, 1210
BmcAI AGTACT 2 cut(s) 625, 962
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 5 cut(s) 389, 439, 881, 954, 1029
BmrFI CCNGG 1 cut(s) 672
BmrI ACTGGG 1 cut(s) 831
BmsI GCATC 1 cut(s) 333
BmuI ACTGGG 1 cut(s) 831
Bpu10I CCTNAGC 1 cut(s) 537
BpuMI CCSGG 1 cut(s) 672
BsaBI GATNNNNATC 1 cut(s) 900
BsaJI CCNNGG 4 cut(s) 277, 328, 433, 651
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse118I RCCGGY 2 cut(s) 315, 390
Bse1I ACTGG 4 cut(s) 621, 826, 1131, 1202
Bse8I GATNNNNATC 1 cut(s) 900
BseDI CCNNGG 4 cut(s) 277, 328, 433, 651
BseGI GGATG 2 cut(s) 324, 831
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMII CTCAG 6 cut(s) 6, 528, 554, 971, 1081, 1085
BseNI ACTGG 4 cut(s) 621, 826, 1131, 1202
BseRI GAGGAG 2 cut(s) 146, 350
BseSI GKGCMC 2 cut(s) 616, 672
BseXI GCAGC 5 cut(s) 364, 676, 869, 1110, 1220
BseYI CCCAGC 1 cut(s) 237
BshFI GGCC 2 cut(s) 1143, 1235
BshNI GGYRCC 1 cut(s) 1027
BshTI ACCGGT 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 3 cut(s) 316, 391, 672
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 1 cut(s) 334
BsmFI GGGAC 1 cut(s) 1065
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 2 cut(s) 1143, 1235
Bsp1286I GDGCHC 2 cut(s) 616, 672
Bsp143I GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 1 cut(s) 748
BspANI GGCC 2 cut(s) 1143, 1235
BspCNI CTCAG 6 cut(s) 7, 529, 555, 970, 1080, 1086
BspLI GGNNCC 5 cut(s) 389, 439, 881, 954, 1029
BspPI GGATC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 1027
BsrFI RCCGGY 2 cut(s) 315, 390
BsrI ACTGG 4 cut(s) 621, 826, 1131, 1202
BssAI RCCGGY 2 cut(s) 315, 390
BssECI CCNNGG 4 cut(s) 277, 328, 433, 651
BssMI GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
BssT1I CCWWGG 3 cut(s) 277, 328, 651
Bst4CI ACNGT 1 cut(s) 618
Bst6I CTCTTC 1 cut(s) 417
BstC8I GCNNGC 3 cut(s) 187, 492, 593
BstDEI CTNAG 7 cut(s) 15, 233, 537, 563, 957, 1067, 1094
BstDSI CCRYGG 2 cut(s) 433, 651
BstF5I GGATG 2 cut(s) 324, 831
BstHHI GCGC 2 cut(s) 67, 104
BstKTI GATC 8 cut(s) 109, 127, 216, 451, 732, 904, 913, 1024
BstMBI GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
BstMWI GCNNNNNNNGC 3 cut(s) 101, 491, 1149
BstNSI RCATGY 2 cut(s) 591, 1243
BstSCI CCNGG 1 cut(s) 670
BstSLI GKGCMC 2 cut(s) 616, 672
BstV1I GCAGC 5 cut(s) 364, 676, 869, 1110, 1220
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 2 cut(s) 1143, 1235
BtgI CCRYGG 2 cut(s) 433, 651
BtgZI GCGATG 1 cut(s) 517
BtsCI GGATG 2 cut(s) 324, 831
BtsIMutI CAGTG 1 cut(s) 304
Cac8I GCNNGC 3 cut(s) 187, 492, 593
CfoI GCGC 2 cut(s) 67, 104
Cfr10I RCCGGY 2 cut(s) 315, 390
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 6 cut(s) 169, 528, 624, 961, 1028, 1278
CspAI ACCGGT 1 cut(s) 315
CviAII CATG 4 cut(s) 588, 652, 1048, 1240
CviQI GTAC 6 cut(s) 169, 528, 624, 961, 1028, 1278
DdeI CTNAG 7 cut(s) 15, 233, 537, 563, 957, 1067, 1094
DpnI GATC 8 cut(s) 108, 126, 215, 450, 731, 903, 912, 1023
DpnII GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
DraIII CACNNNGTG 1 cut(s) 1217
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Eco130I CCWWGG 3 cut(s) 277, 328, 651
Eco47I GGWCC 1 cut(s) 274
Eco57I CTGAAG 3 cut(s) 390, 441, 683
EcoRI GAATTC 1 cut(s) 255
EcoT14I CCWWGG 3 cut(s) 277, 328, 651
ErhI CCWWGG 3 cut(s) 277, 328, 651
FaeI CATG 4 cut(s) 591, 655, 1051, 1243
FalI AAGNNNNNCTT 2 cut(s) 474, 506
FaqI GGGAC 1 cut(s) 1065
FatI CATG 4 cut(s) 587, 651, 1047, 1239
FauNDI CATATG 1 cut(s) 598
FbaI TGATCA 3 cut(s) 448, 729, 1021
FblI GTMKAC 1 cut(s) 1003
Fnu4HI GCNGC 5 cut(s) 378, 690, 858, 1099, 1209
FokI GGATG 2 cut(s) 311, 838
Fsp4HI GCNGC 5 cut(s) 378, 690, 858, 1099, 1209
FspBI CTAG 7 cut(s) 53, 306, 399, 977, 1149, 1205, 1275
FspI TGCGCA 1 cut(s) 66
GlaI GCGC 2 cut(s) 66, 103
GluI GCNGC 5 cut(s) 378, 690, 858, 1099, 1209
GsaI CCCAGC 1 cut(s) 241
HaeIII GGCC 2 cut(s) 1143, 1235
HapII CCGG 3 cut(s) 316, 391, 672
HhaI GCGC 2 cut(s) 67, 104
Hin1II CATG 4 cut(s) 591, 655, 1051, 1243
Hin6I GCGC 2 cut(s) 65, 102
HinP1I GCGC 2 cut(s) 65, 102
HinfI GANTC 2 cut(s) 428, 813
HpaII CCGG 3 cut(s) 316, 391, 672
HphI GGTGA 3 cut(s) 625, 769, 982
Hpy166II GTNNAC 4 cut(s) 74, 528, 614, 1004
Hpy188I TCNGA 7 cut(s) 16, 421, 460, 564, 818, 1095, 1162
Hpy188III TCNNGA 2 cut(s) 128, 1184
Hpy8I GTNNAC 4 cut(s) 74, 528, 614, 1004
HpyAV CCTTC 2 cut(s) 414, 514
HpyCH4III ACNGT 1 cut(s) 618
HpyCH4V TGCA 8 cut(s) 138, 185, 380, 494, 614, 857, 1178, 1250
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 491, 1149
HpyF3I CTNAG 7 cut(s) 15, 233, 537, 563, 957, 1067, 1094
Hsp92II CATG 4 cut(s) 591, 655, 1051, 1243
HspAI GCGC 2 cut(s) 65, 102
KpnI GGTACC 1 cut(s) 1031
Ksp22I TGATCA 3 cut(s) 448, 729, 1021
Kzo9I GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
LmnI GCTCC 1 cut(s) 885
Lsp1109I GCAGC 5 cut(s) 364, 676, 869, 1110, 1220
LweI GCATC 1 cut(s) 333
MaeI CTAG 7 cut(s) 53, 306, 399, 977, 1149, 1205, 1275
MaeIII GTNAC 1 cut(s) 1200
MalI GATC 8 cut(s) 108, 126, 215, 450, 731, 903, 912, 1023
MboI GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
MboII GAAGA 8 cut(s) 3, 28, 31, 148, 434, 520, 622, 1006
MfeI CAATTG 1 cut(s) 160
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 2 cut(s) 616, 672
MluCI AATT 7 cut(s) 160, 255, 357, 464, 502, 927, 1037
MmeI TCCRAC 1 cut(s) 1185
MseI TTAA 3 cut(s) 383, 1040, 1170
MslI CAYNNNNRTG 2 cut(s) 725, 845
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 3 cut(s) 316, 391, 672
MspR9I CCNGG 1 cut(s) 672
MunI CAATTG 1 cut(s) 160
Mva1269I GAATGC 1 cut(s) 187
MwoI GCNNNNNNNGC 3 cut(s) 101, 491, 1149
NciI CCSGG 1 cut(s) 672
NcoI CCATGG 1 cut(s) 651
NdeI CATATG 1 cut(s) 598
NdeII GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
NlaIII CATG 4 cut(s) 591, 655, 1051, 1243
NlaIV GGNNCC 5 cut(s) 389, 439, 881, 954, 1029
NsbI TGCGCA 1 cut(s) 66
NspI RCATGY 2 cut(s) 591, 1243
PciI ACATGT 1 cut(s) 1239
PctI GAATGC 1 cut(s) 187
PfeI GAWTC 2 cut(s) 428, 813
PinAI ACCGGT 1 cut(s) 315
PkrI GCNGC 5 cut(s) 379, 691, 859, 1100, 1210
PscI ACATGT 1 cut(s) 1239
PspFI CCCAGC 1 cut(s) 237
PspN4I GGNNCC 5 cut(s) 389, 439, 881, 954, 1029
PspPI GGNCC 1 cut(s) 274
PsuI RGATCY 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 6 cut(s) 170, 529, 625, 962, 1029, 1279
RsaNI GTAC 6 cut(s) 169, 528, 624, 961, 1028, 1278
RseI CAYNNNNRTG 2 cut(s) 725, 845
SaqAI TTAA 3 cut(s) 383, 1040, 1170
SatI GCNGC 5 cut(s) 378, 690, 858, 1099, 1209
Sau3AI GATC 8 cut(s) 106, 124, 213, 448, 729, 901, 910, 1021
Sau96I GGNCC 1 cut(s) 274
ScaI AGTACT 2 cut(s) 625, 962
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 2 cut(s) 616, 672
SfaNI GCATC 1 cut(s) 333
SinI GGWCC 1 cut(s) 274
SmiMI CAYNNNNRTG 2 cut(s) 725, 845
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 7 cut(s) 160, 255, 357, 464, 502, 927, 1037
SsiI CCGC 1 cut(s) 748
SspMI CTAG 7 cut(s) 53, 306, 399, 977, 1149, 1205, 1275
StyD4I CCNGG 1 cut(s) 670
StyI CCWWGG 3 cut(s) 277, 328, 651
TaaI ACNGT 1 cut(s) 618
TasI AATT 7 cut(s) 160, 255, 357, 464, 502, 927, 1037
TatI WGTACW 3 cut(s) 623, 960, 1277
TfiI GAWTC 2 cut(s) 428, 813
Tru1I TTAA 3 cut(s) 383, 1040, 1170
Tru9I TTAA 3 cut(s) 383, 1040, 1170
TscAI CASTG 1 cut(s) 304
TseI GCWGC 5 cut(s) 377, 689, 857, 1098, 1208
TspDTI ATGAA 3 cut(s) 194, 887, 1050
TspRI CASTG 1 cut(s) 304
VneI GTGCAC 1 cut(s) 612
VpaK11BI GGWCC 1 cut(s) 274
XapI RAATTY 1 cut(s) 255
XceI RCATGY 2 cut(s) 591, 1243
XcmI CCANNNNNNNNNTGG 1 cut(s) 939
XmiI GTMKAC 1 cut(s) 1003
XspI CTAG 7 cut(s) 53, 306, 399, 977, 1149, 1205, 1275
ZrmI AGTACT 2 cut(s) 625, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.