MD02G1261500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000424
Physical Location & Seq
Forward (+)
31599558 .. 31601279
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1261500.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGTCAGACTTCCCACCGGAGATACTGTTCGACATCCTATCACGCCTTCCGCCCAAGGATGTGATTCGATTCCTCTGCGTTTCCAAAGCTTGGAATGCCCTCATCCGCCACCACCGCTTCATCGAGGCTCATCTTCAACGCTCCATCCAAACCAATTCTTTTCGCACCATTTTACTCGAGTCGGAAGATGGCTCTCGCCCTTCAAATTTCTTTTCATCGGCTTTCGACGACAGCGAAACGTTCGGGCCAGTCGTCAAAATTGAGCAGCCGTTGAAATGCCCTGACGACTATACTGAGATATTGGGCTGCTCAATTAATGGTGTGGTTTGCGTTCACAACGGAATGCGTAAGAATCTTGCTTTGTGGAACCCGTCAATTCAAAAGTTCAAGAAGATTCCTCTCCCAACCTTTGAGGTTGAGCGGCGGCGATCCTCATTCTCGGCGATCCCTGAATATGGATTCGGGTACGATTCAGCTAATCACGATTATAAAATTTTGGCAATTGTGAATTTTGATCGGACGGATGATGCTTCTGTGCCTGTGAGCTCTCAAGTCAGTATCTATAGCCTAAAATTCAACTCATGGAAAAGGATTCCAAAATTGCCTTGCGATGGTTTCTATGTGCAGACGGGCGATGTGGTGTTTTTGAACGGTGCTATGAGTTGTTTGGTGCGGAAATCAGATTCATATAAAAATCGATGCAAAATTGTATCCCTTGATCTTGCCAGTGAGAAGTATATGGAGTTTGGCACCCCGGTGGGGGATGAGGATGACAATTTTATGATGAGTTTGAAGGTCTTGGGAGGCTCTCTATGTATTTGTGTTCATGAGTTTTGGCCAAAATGTGATATTTGGATTATGAAGGAATACGGAGTCACAAAATCTTGGACCTTGCTTTTTCCTTTTGAGAATGGAGGTATGGCTTATTCCAACAGATATTGCAAACCTTTGGTGTTCTCAAAGGAGGGTGAAATGCTTGTTCTTGTGAATGTAGAGCGGACTACCGTTTTTCGGTGTGATTTAAAGAAAAAGAGAGCCAAACAAGTTCGAATTTGTGGTCTGCCGACTTCCTTCAATGGAACCATTTGTGTGGGGAGCCTTTCTCTTCTTGACGGCGATCTGATGACTGTTGGAAGGCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

382

Amino Acids

43.26

Weight (kDa)

8.3

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 40 2.9e-11 F-box domain
F-box-like PF12937 2 - 38 6e-09 F-box-like
FBA_3 PF08268 68 - 357 5.4e-19 F-box associated beta propeller domain
FBA_1 PF07734 79 - 333 3.6e-22 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AccB1I GGYRCC 1 cut(s) 749
AccB7I CCANNNNNTGG 1 cut(s) 90
AccBSI CCGCTC 2 cut(s) 421, 997
AciI CCGC 7 cut(s) 50, 106, 115, 421, 424, 673, 997
AclI AACGTT 1 cut(s) 239
AclWI GGATC 2 cut(s) 423, 439
AcoI YGGCCR 1 cut(s) 836
AcsI RAATTY 5 cut(s) 205, 492, 508, 572, 1050
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 6 cut(s) 90, 455, 611, 759, 760, 1011
AgsI TTSAA 9 cut(s) 137, 204, 274, 380, 388, 577, 649, 793, 1075
AleI CACNNNNGTG 1 cut(s) 755
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 3 cut(s) 89, 476, 546
AluI AGCT 3 cut(s) 89, 476, 546
Alw21I GWGCWC 1 cut(s) 548
AlwI GGATC 2 cut(s) 423, 439
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 2 cut(s) 245, 836
ApeKI GCWGC 3 cut(s) 265, 306, 1138
ApoI RAATTY 5 cut(s) 205, 492, 508, 572, 1050
ArsI GACNNNNNNTTYG 2 cut(s) 1042, 1074
AseI ATTAAT 1 cut(s) 315
AspS9I GGNCC 2 cut(s) 245, 888
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 1 cut(s) 980
AsuII TTCGAA 1 cut(s) 1048
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 888
BalI TGGCCA 1 cut(s) 838
BanI GGYRCC 1 cut(s) 749
BanII GRGCYC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 548
BbvI GCAGC 2 cut(s) 277, 293
BccI CCATC 3 cut(s) 152, 182, 605
BceAI ACGGC 2 cut(s) 253, 1129
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciVI GTATCC 1 cut(s) 721
BcnI CCSGG 1 cut(s) 755
BfmI CTRYAG 1 cut(s) 562
BfuI GTATCC 1 cut(s) 721
BisI GCNGC 5 cut(s) 266, 307, 422, 425, 1139
BlsI GCNGC 5 cut(s) 267, 308, 423, 426, 1140
Bme1390I CCNGG 1 cut(s) 755
Bme18I GGWCC 1 cut(s) 888
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 2 cut(s) 245, 888
BmiI GGNNCC 4 cut(s) 368, 751, 1081, 1097
BmrFI CCNGG 1 cut(s) 755
BmsI GCATC 2 cut(s) 517, 689
BplI GAGNNNNNCTC 2 cut(s) 1087, 1119
Bpu14I TTCGAA 1 cut(s) 1048
BpuEI CTTGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 755
Bsa29I ATCGAT 1 cut(s) 697
BsaJI CCNNGG 2 cut(s) 54, 753
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 6 cut(s) 90, 455, 611, 759, 760, 1011
Bse1I ACTGG 2 cut(s) 248, 726
BseCI ATCGAT 1 cut(s) 697
BseDI CCNNGG 2 cut(s) 54, 753
BseGI GGATG 7 cut(s) 33, 64, 102, 144, 529, 769, 775
BseLI CCNNNNNNNGG 6 cut(s) 90, 455, 611, 759, 760, 1011
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 248, 726
BseXI GCAGC 2 cut(s) 277, 293
BsgI GTGCAG 1 cut(s) 644
BshFI GGCC 2 cut(s) 247, 838
BshNI GGYRCC 1 cut(s) 749
BshVI ATCGAT 1 cut(s) 697
BsiHKAI GWGCWC 1 cut(s) 548
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 17, 755
BslI CCNNNNNNNGG 6 cut(s) 90, 455, 611, 759, 760, 1011
BsmI GAATGC 2 cut(s) 100, 348
BsnI GGCC 2 cut(s) 247, 838
BsoBI CYCGRG 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 1048
Bsp1286I GDGCHC 1 cut(s) 548
Bsp143I GATC 5 cut(s) 428, 444, 514, 718, 1117
BspACI CCGC 7 cut(s) 50, 106, 115, 421, 424, 673, 997
BspANI GGCC 2 cut(s) 247, 838
BspCNI CTCAG 1 cut(s) 286
BspDI ATCGAT 1 cut(s) 697
BspHI TCATGA 1 cut(s) 826
BspLI GGNNCC 4 cut(s) 368, 751, 1081, 1097
BspPI GGATC 2 cut(s) 423, 439
BspT104I TTCGAA 1 cut(s) 1048
BspT107I GGYRCC 1 cut(s) 749
BsrBI CCGCTC 2 cut(s) 421, 997
BsrI ACTGG 2 cut(s) 248, 726
BssECI CCNNGG 2 cut(s) 54, 753
BssMI GATC 5 cut(s) 428, 444, 514, 718, 1117
BssT1I CCWWGG 1 cut(s) 54
Bst4CI ACNGT 4 cut(s) 27, 653, 1006, 1129
Bst6I CTCTTC 1 cut(s) 1110
BstBI TTCGAA 1 cut(s) 1048
BstDEI CTNAG 1 cut(s) 294
BstF5I GGATG 7 cut(s) 33, 64, 102, 144, 529, 769, 775
BstKTI GATC 5 cut(s) 431, 447, 517, 721, 1120
BstMBI GATC 5 cut(s) 428, 444, 514, 718, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 95, 114
BstSCI CCNGG 1 cut(s) 753
BstSFI CTRYAG 1 cut(s) 562
BstV1I GCAGC 2 cut(s) 277, 293
BstXI CCANNNNNNTGG 1 cut(s) 1090
Bsu15I ATCGAT 1 cut(s) 697
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 2 cut(s) 247, 838
BsuTUI ATCGAT 1 cut(s) 697
BtgZI GCGATG 2 cut(s) 624, 648
BtsCI GGATG 7 cut(s) 33, 64, 102, 144, 529, 769, 775
BtsIMutI CAGTG 1 cut(s) 733
CciI TCATGA 1 cut(s) 826
Cfr13I GGNCC 2 cut(s) 245, 888
ClaI ATCGAT 1 cut(s) 697
Csp6I GTAC 1 cut(s) 466
CviAII CATG 2 cut(s) 582, 827
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 1 cut(s) 294
DpnI GATC 5 cut(s) 430, 446, 516, 720, 1119
DpnII GATC 5 cut(s) 428, 444, 514, 718, 1117
DraI TTTAAA 1 cut(s) 1023
EaeI YGGCCR 1 cut(s) 836
Eam1104I CTCTTC 1 cut(s) 1110
EarI CTCTTC 1 cut(s) 1110
EciI GGCGGA 2 cut(s) 39, 95
Ecl136II GAGCTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 888
Eco53kI GAGCTC 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 546
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 2 cut(s) 585, 830
FatI CATG 2 cut(s) 581, 826
Fnu4HI GCNGC 5 cut(s) 266, 307, 422, 425, 1139
FokI GGATG 7 cut(s) 20, 71, 89, 131, 536, 776, 782
FriOI GRGCYC 1 cut(s) 548
Fsp4HI GCNGC 5 cut(s) 266, 307, 422, 425, 1139
GluI GCNGC 5 cut(s) 266, 307, 422, 425, 1139
HaeIII GGCC 2 cut(s) 247, 838
HapII CCGG 2 cut(s) 17, 755
Hin1II CATG 2 cut(s) 585, 830
HindIII AAGCTT 1 cut(s) 87
HpaII CCGG 2 cut(s) 17, 755
HphI GGTGA 1 cut(s) 980
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 5 cut(s) 7, 184, 519, 682, 1122
Hpy188III TCNNGA 4 cut(s) 388, 482, 827, 1109
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 6 cut(s) 56, 210, 787, 856, 1081, 1128
HpyCH4III ACNGT 4 cut(s) 27, 653, 1006, 1129
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 3 cut(s) 625, 702, 942
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 114
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 2 cut(s) 585, 830
Kzo9I GATC 5 cut(s) 428, 444, 514, 718, 1117
LmnI GCTCC 2 cut(s) 146, 1095
LpnPI CCDG 7 cut(s) 30, 261, 294, 462, 552, 739, 768
Lsp1109I GCAGC 2 cut(s) 277, 293
LweI GCATC 2 cut(s) 517, 689
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 874
MalI GATC 5 cut(s) 430, 446, 516, 720, 1119
MbiI CCGCTC 2 cut(s) 421, 997
MboI GATC 5 cut(s) 428, 444, 514, 718, 1117
MboII GAAGA 4 cut(s) 125, 197, 403, 1097
MfeI CAATTG 1 cut(s) 501
MhlI GDGCHC 1 cut(s) 548
MlsI TGGCCA 1 cut(s) 838
MluNI TGGCCA 1 cut(s) 838
MlyI GAGTC 2 cut(s) 188, 882
MmeI TCCRAC 3 cut(s) 162, 954, 1111
Mox20I TGGCCA 1 cut(s) 838
MscI TGGCCA 1 cut(s) 838
MseI TTAA 2 cut(s) 315, 1022
MslI CAYNNNNRTG 2 cut(s) 755, 1088
Msp20I TGGCCA 1 cut(s) 838
MspI CCGG 2 cut(s) 17, 755
MspR9I CCNGG 1 cut(s) 755
MunI CAATTG 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 100, 348
MwoI GCNNNNNNNGC 2 cut(s) 95, 114
NciI CCSGG 1 cut(s) 755
NdeII GATC 5 cut(s) 428, 444, 514, 718, 1117
NlaIII CATG 2 cut(s) 585, 830
NlaIV GGNNCC 4 cut(s) 368, 751, 1081, 1097
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 874
NspV TTCGAA 1 cut(s) 1048
OliI CACNNNNGTG 1 cut(s) 755
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 826
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 249
PctI GAATGC 2 cut(s) 100, 348
PfeI GAWTC 8 cut(s) 64, 69, 352, 394, 459, 470, 592, 683
PflMI CCANNNNNTGG 1 cut(s) 90
PkrI GCNGC 5 cut(s) 267, 308, 423, 426, 1140
PleI GAGTC 2 cut(s) 187, 881
PpsI GAGTC 2 cut(s) 187, 881
PshBI ATTAAT 1 cut(s) 315
PsiI TTATAA 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 548
Psp1406I AACGTT 1 cut(s) 239
PspN4I GGNNCC 4 cut(s) 368, 751, 1081, 1097
PspPI GGNCC 2 cut(s) 245, 888
PspXI VCTCGAGB 1 cut(s) 176
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 2 cut(s) 755, 1088
SacI GAGCTC 1 cut(s) 548
SaqAI TTAA 2 cut(s) 315, 1022
SatI GCNGC 5 cut(s) 266, 307, 422, 425, 1139
Sau3AI GATC 5 cut(s) 428, 444, 514, 718, 1117
Sau96I GGNCC 2 cut(s) 245, 888
SchI GAGTC 2 cut(s) 188, 882
ScrFI CCNGG 1 cut(s) 755
SduI GDGCHC 1 cut(s) 548
SfaNI GCATC 2 cut(s) 517, 689
SfcI CTRYAG 1 cut(s) 562
Sfr274I CTCGAG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 1048
SinI GGWCC 1 cut(s) 888
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 2 cut(s) 755, 1088
SmlI CTYRAG 2 cut(s) 176, 549
SmoI CTYRAG 2 cut(s) 176, 549
SsiI CCGC 7 cut(s) 50, 106, 115, 421, 424, 673, 997
SstI GAGCTC 1 cut(s) 548
StyD4I CCNGG 1 cut(s) 753
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 27, 653, 1006, 1129
TaiI ACGT 1 cut(s) 242
TaqI TCGA 7 cut(s) 30, 67, 123, 177, 225, 697, 1048
TauI GCSGC 2 cut(s) 424, 427
TfiI GAWTC 8 cut(s) 64, 69, 352, 394, 459, 470, 592, 683
Tru1I TTAA 2 cut(s) 315, 1022
Tru9I TTAA 2 cut(s) 315, 1022
TscAI CASTG 1 cut(s) 733
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 3 cut(s) 265, 306, 1138
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 5 cut(s) 109, 204, 675, 815, 875
TspGWI ACGGA 3 cut(s) 354, 536, 885
TspRI CASTG 1 cut(s) 733
Van91I CCANNNNNTGG 1 cut(s) 90
VpaK11BI GGWCC 1 cut(s) 888
VspI ATTAAT 1 cut(s) 315
XapI RAATTY 5 cut(s) 205, 492, 508, 572, 1050
XhoI CTCGAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.