MD16G1218300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000424
Physical Location & Seq
Forward (+)
21653520 .. 21654632
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1218300.v1.1.491

Sequence Viewer

Length: 957 bp
ATGTCAAGTTTTCCACCGGAGCTCGTCGTCGACATCCTTTCATGGCTTGCTCCGGAGGATTTGATACGATGCATGCGTGTTTGCAAAGCTTGGAATGCTTTCATCCGCGGTGAAAACTTCATCAAATCCCACCTCCAACGCTCCATCCAAACCAACTCCACTCGCTTCATTTTACTCACCTCATCTGATTCGGTTTTATTTTCCTTGCCTTTCGGCGGCAAGGAGACGTTCGGGTCAGCCAATGGGGGCTTTGCTTTGTGGAATCCATCCATCCGAAAGTTGAAGAAGATTCCGCGCAGCACACTTGAGCCGCTGCCACCCCTGGTTTCGGGGCATTCTGCCTTGTATGGATTCGGGTATGATTCCGCTAATGCTGATTATAATCTTGTGGGAGTAATAGAGTTTTACAATTTGAAGAATGTTATTGTGAGTTCTCGTGTTGAACTTTATAGCCTAAAATCTAACTCATGGAAAAGCATCCAAAACATGCCTTGCGATGGATTTAAAGTATATCCAATTTGTTCACGTCGTTTATTGTTTATAAGTGGTGCTCTTTGTTGGCTGATGCATAATAATACAAATCGGTTGATAATTCTATCCCTTGATCTTGCAAGTGAGAAATATCGCCAATTTCCCACCCCATATGTTGGGAATGGCCGCATTTATCTGCTTCTAGATGAATATGGAGTGGCAGAATCTTGGACTTTTCTTTTTTCTGTTGAGATGGAAGTTCTGCCTATGGGGTTTGATTTACTTTGCAAACTTTTGGCTTTCTTAAAGAATGGTGACCTGGTTCTTTTGACGAATCACAATGGTGGTATTTATTGGTATGACTTAGAGGAAAAAAGTTTCAAACAAGTTGAAATTCATGGTGAACCAAGTACCTACATAGCAACCGTCGGTGTGGGAAGCCTTTGTCTTCTTGATGGCGATTCTGTAGTTGCTAAGAGGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

319

Amino Acids

35.78

Weight (kDa)

7.57

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 41 2.7e-09 F-box domain
F-box-like PF12937 3 - 38 9.1e-11 F-box-like
FBA_3 PF08268 78 - 214 2.9e-07 F-box associated beta propeller domain
FBA_1 PF07734 82 - 214 1.2e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 381, 542
AasI GACNNNNNNGTC 1 cut(s) 232
AccB7I CCANNNNNTGG 1 cut(s) 647
AccI GTMKAC 1 cut(s) 30
AccII CGCG 2 cut(s) 108, 295
AccIII TCCGGA 1 cut(s) 52
AciI CCGC 7 cut(s) 106, 108, 216, 293, 311, 366, 658
AcoI YGGCCR 1 cut(s) 655
AcsI RAATTY 1 cut(s) 864
AfaI GTAC 1 cut(s) 883
AfiI CCNNNNNNNGG 4 cut(s) 215, 328, 497, 647
AgsI TTSAA 5 cut(s) 283, 415, 443, 853, 863
AjiI CACGTC 1 cut(s) 527
AjnI CCWGG 2 cut(s) 321, 789
AleI CACNNNNGTG 1 cut(s) 813
AluBI AGCT 2 cut(s) 22, 89
AluI AGCT 2 cut(s) 22, 89
Alw21I GWGCWC 2 cut(s) 24, 553
Alw26I GTCTC 1 cut(s) 218
Aor13HI TCCGGA 1 cut(s) 52
AoxI GGCC 1 cut(s) 655
ApeKI GCWGC 2 cut(s) 297, 313
ApoI RAATTY 1 cut(s) 864
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 297
AsuHPI GGTGA 4 cut(s) 122, 169, 797, 884
BanII GRGCYC 1 cut(s) 24
BauI CACGAG 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 911
Bbv12I GWGCWC 2 cut(s) 24, 553
BbvI GCAGC 2 cut(s) 300, 309
BccI CCATC 6 cut(s) 152, 274, 278, 491, 718, 920
BciT130I CCWGG 2 cut(s) 323, 791
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 1 cut(s) 674
BfmI CTRYAG 1 cut(s) 936
BisI GCNGC 5 cut(s) 217, 298, 311, 314, 658
BlsI GCNGC 5 cut(s) 218, 299, 312, 315, 659
Bme1390I CCNGG 2 cut(s) 323, 791
BmgBI CACGTC 1 cut(s) 527
BmrFI CCNGG 2 cut(s) 323, 791
BmsI GCATC 3 cut(s) 59, 486, 555
BpiI GAAGAC 1 cut(s) 911
BpuEI CTTGAG 1 cut(s) 326
BsaBI GATNNNNATC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 106, 321
BsaWI WCCGGW 2 cut(s) 16, 52
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 328, 497, 647
Bse8I GATNNNNATC 1 cut(s) 381
BseAI TCCGGA 1 cut(s) 52
BseBI CCWGG 2 cut(s) 323, 791
BseDI CCNNGG 2 cut(s) 106, 321
BseGI GGATG 6 cut(s) 33, 102, 144, 266, 270, 477
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 4 cut(s) 215, 328, 497, 647
BseXI GCAGC 2 cut(s) 300, 309
Bsh1236I CGCG 2 cut(s) 108, 295
BshFI GGCC 1 cut(s) 657
BsiHKAI GWGCWC 2 cut(s) 24, 553
BsiSI CCGG 2 cut(s) 17, 53
BslI CCNNNNNNNGG 4 cut(s) 215, 328, 497, 647
BsmAI GTCTC 1 cut(s) 218
BsmBI CGTCTC 1 cut(s) 218
BsmI GAATGC 2 cut(s) 100, 334
BsnI GGCC 1 cut(s) 657
Bsp1286I GDGCHC 2 cut(s) 24, 553
Bsp13I TCCGGA 1 cut(s) 52
Bsp143I GATC 1 cut(s) 604
BspACI CCGC 7 cut(s) 106, 108, 216, 293, 311, 366, 658
BspANI GGCC 1 cut(s) 657
BspEI TCCGGA 1 cut(s) 52
BspFNI CGCG 2 cut(s) 108, 295
BssECI CCNNGG 2 cut(s) 106, 321
BssMI GATC 1 cut(s) 604
BssSI CACGAG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 323, 791
Bst4CI ACNGT 1 cut(s) 898
BstC8I GCNNGC 2 cut(s) 48, 74
BstDEI CTNAG 2 cut(s) 835, 945
BstDSI CCRYGG 1 cut(s) 106
BstEII GGTNACC 1 cut(s) 785
BstF5I GGATG 6 cut(s) 33, 102, 144, 266, 270, 477
BstFNI CGCG 2 cut(s) 108, 295
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 1 cut(s) 607
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 1 cut(s) 604
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstNI CCWGG 2 cut(s) 323, 791
BstNSI RCATGY 2 cut(s) 76, 490
BstPI GGTNACC 1 cut(s) 785
BstSCI CCNGG 2 cut(s) 321, 789
BstSFI CTRYAG 1 cut(s) 936
BstUI CGCG 2 cut(s) 108, 295
BstV1I GCAGC 2 cut(s) 300, 309
BstV2I GAAGAC 1 cut(s) 911
BsuRI GGCC 1 cut(s) 657
BtgI CCRYGG 1 cut(s) 106
BtgZI GCGATG 1 cut(s) 510
BtrI CACGTC 1 cut(s) 527
BtsCI GGATG 6 cut(s) 33, 102, 144, 266, 270, 477
Cac8I GCNNGC 2 cut(s) 48, 74
CfoI GCGC 1 cut(s) 297
Cfr42I CCGCGG 1 cut(s) 109
CsiI ACCWGGT 1 cut(s) 789
Csp6I GTAC 1 cut(s) 882
CviAII CATG 5 cut(s) 42, 73, 468, 487, 869
CviQI GTAC 1 cut(s) 882
DdeI CTNAG 2 cut(s) 835, 945
DpnI GATC 1 cut(s) 606
DpnII GATC 1 cut(s) 604
DraI TTTAAA 1 cut(s) 505
DrdI GACNNNNNNGTC 1 cut(s) 232
DseDI GACNNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 655
Ecl136II GAGCTC 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 24
Eco53kI GAGCTC 1 cut(s) 22
Eco91I GGTNACC 1 cut(s) 785
EcoICRI GAGCTC 1 cut(s) 22
EcoO65I GGTNACC 1 cut(s) 785
EcoRII CCWGG 2 cut(s) 321, 789
EcoT22I ATGCAT 2 cut(s) 74, 570
EcoT38I GRGCYC 1 cut(s) 24
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 5 cut(s) 45, 76, 471, 490, 872
FatI CATG 5 cut(s) 41, 72, 467, 486, 868
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 5 cut(s) 217, 298, 311, 314, 658
FokI GGATG 6 cut(s) 20, 89, 131, 253, 257, 464
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 5 cut(s) 217, 298, 311, 314, 658
FspBI CTAG 1 cut(s) 674
GlaI GCGC 1 cut(s) 296
GluI GCNGC 5 cut(s) 217, 298, 311, 314, 658
HaeIII GGCC 1 cut(s) 657
HapII CCGG 2 cut(s) 17, 53
HhaI GCGC 1 cut(s) 297
Hin1II CATG 5 cut(s) 45, 76, 471, 490, 872
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 8 cut(s) 188, 262, 289, 351, 362, 695, 805, 932
HpaII CCGG 2 cut(s) 17, 53
HphI GGTGA 4 cut(s) 122, 169, 797, 884
Hpy166II GTNNAC 3 cut(s) 31, 524, 875
Hpy188I TCNGA 2 cut(s) 187, 275
Hpy188III TCNNGA 3 cut(s) 53, 674, 923
Hpy8I GTNNAC 3 cut(s) 31, 524, 875
Hpy99I CGWCG 4 cut(s) 29, 32, 531, 902
HpyCH4III ACNGT 1 cut(s) 898
HpyCH4IV ACGT 2 cut(s) 227, 526
HpyCH4V TGCA 5 cut(s) 72, 84, 568, 611, 759
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpyF3I CTNAG 2 cut(s) 835, 945
HpySE526I ACGT 2 cut(s) 227, 526
Hsp92II CATG 5 cut(s) 45, 76, 471, 490, 872
HspAI GCGC 1 cut(s) 295
Kpn2I TCCGGA 1 cut(s) 52
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 1 cut(s) 604
LmnI GCTCC 3 cut(s) 19, 55, 146
LpnPI CCDG 6 cut(s) 30, 66, 308, 335, 776, 803
Lsp1109I GCAGC 2 cut(s) 300, 309
LweI GCATC 3 cut(s) 59, 486, 555
MabI ACCWGGT 1 cut(s) 789
MaeI CTAG 1 cut(s) 674
MaeII ACGT 2 cut(s) 227, 526
MaeIII GTNAC 1 cut(s) 785
MalI GATC 1 cut(s) 606
MboI GATC 1 cut(s) 604
MboII GAAGA 4 cut(s) 295, 298, 427, 911
MhlI GDGCHC 2 cut(s) 24, 553
MluCI AATT 5 cut(s) 409, 516, 591, 629, 864
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 5 cut(s) 49, 143, 190, 832, 942
Mph1103I ATGCAT 2 cut(s) 74, 570
MroI TCCGGA 1 cut(s) 52
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 2 cut(s) 504, 776
MslI CAYNNNNRTG 1 cut(s) 813
MspA1I CMGCKG 2 cut(s) 108, 313
MspI CCGG 2 cut(s) 17, 53
MspR9I CCNGG 2 cut(s) 323, 791
Mva1269I GAATGC 2 cut(s) 100, 334
MvaI CCWGG 2 cut(s) 323, 791
MvnI CGCG 2 cut(s) 108, 295
MwoI GCNNNNNNNGC 1 cut(s) 95
NdeI CATATG 1 cut(s) 643
NdeII GATC 1 cut(s) 604
NlaIII CATG 5 cut(s) 45, 76, 471, 490, 872
NmuCI GTSAC 1 cut(s) 785
NsiI ATGCAT 2 cut(s) 74, 570
NspI RCATGY 2 cut(s) 76, 490
OliI CACNNNNGTG 1 cut(s) 813
PaeI GCATGC 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 73
PctI GAATGC 2 cut(s) 100, 334
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 8 cut(s) 188, 262, 289, 351, 362, 695, 805, 932
PflMI CCANNNNNTGG 1 cut(s) 647
PkrI GCNGC 5 cut(s) 218, 299, 312, 315, 659
PsiI TTATAA 2 cut(s) 381, 542
Psp124BI GAGCTC 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 321, 789
PspEI GGTNACC 1 cut(s) 785
PspGI CCWGG 2 cut(s) 321, 789
RsaI GTAC 1 cut(s) 883
RsaNI GTAC 1 cut(s) 882
RseI CAYNNNNRTG 1 cut(s) 813
SacI GAGCTC 1 cut(s) 24
SacII CCGCGG 1 cut(s) 109
SalI GTCGAC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 504, 776
SatI GCNGC 5 cut(s) 217, 298, 311, 314, 658
Sau3AI GATC 1 cut(s) 604
ScrFI CCNGG 2 cut(s) 323, 791
SduI GDGCHC 2 cut(s) 24, 553
SetI ASST 8 cut(s) 24, 91, 135, 182, 230, 529, 792, 887
SexAI ACCWGGT 1 cut(s) 789
SfaNI GCATC 3 cut(s) 59, 486, 555
SfcI CTRYAG 1 cut(s) 936
Sfr303I CCGCGG 1 cut(s) 109
SgrBI CCGCGG 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 813
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SphI GCATGC 1 cut(s) 76
Sse9I AATT 5 cut(s) 409, 516, 591, 629, 864
SsiI CCGC 7 cut(s) 106, 108, 216, 293, 311, 366, 658
SspMI CTAG 1 cut(s) 674
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 321, 789
TaaI ACNGT 1 cut(s) 898
TaiI ACGT 2 cut(s) 230, 529
TaqI TCGA 1 cut(s) 30
TasI AATT 5 cut(s) 409, 516, 591, 629, 864
TauI GCSGC 3 cut(s) 219, 313, 660
TfiI GAWTC 8 cut(s) 188, 262, 289, 351, 362, 695, 805, 932
Tru1I TTAA 2 cut(s) 504, 776
Tru9I TTAA 2 cut(s) 504, 776
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 2 cut(s) 297, 313
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 6 cut(s) 30, 91, 109, 157, 693, 857
Van91I CCANNNNNTGG 1 cut(s) 647
XapI RAATTY 1 cut(s) 864
XbaI TCTAGA 1 cut(s) 673
XceI RCATGY 2 cut(s) 76, 490
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 1 cut(s) 674
Zsp2I ATGCAT 2 cut(s) 74, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.