MD02G1290400.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000424
Physical Location & Seq
Reverse (-)
34625059 .. 34626243
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1290400.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGCGGAGTTCCCACCGGAGCTGATCTTTGAAATCCTTTTACGGCTCCCGCCCGAGGACTTGTTACGATGCTTGTGTGTTTCCAAATCTTGGAATGCCACCATCCATGACAAAAAATTTATCAAATCCCATCAACGCTCCATCCAAACCAATTCTTTTGGCTCTCTTTTAGTAACTGCAGGTAATTCCCACGACGGGAGGAAGTTGAAGTTCTCTAATATTTACAACGATGGTACTATAGAACGAGCCACGGGAATCGGACAGTCGCCTCTGCTAGACGTAGAAGGCTGGACTCGTTTTCCTTACACGTTGGTCTCATGCAATGGGATAATTTGCATTCGCACCGAGACGGTCGACAAGGTCGAGGAATTTGTTTTGTGGAATCCGTCAATTCAAAAGTTCAAAAAGATACCCTCCCCAACGTTTGAGCAGCCTCCATCGTCAGATTTATATTTGCACCGTTCCTATGGATTCGGGTATGATTCAGCAAACGACGACTATAAAATTTTGGGAATTGCAAGGTTTTTTGTTAAAGGGGACTTGAATATTGCTGTGCACCAATATCAAATTTATAGCCTAAAATCCAACTCATGGAGAAAGATAAAAAACATGCCGCGCGATGAGTTTCAATTAGGTATGAGTGATATCGTGTTTTCGAACGGTTCTTTAAGTTGGCAGGCATATAACGTATTAGATGAAAAATTCTATGTTGTCACCTTTGAGCTTGCCAGTGAGAAATATCACTGGTTTCCCAACCCGGTTGATAGGGATGGTGATATTTGGCTTGTAGAGGTCTTGGGAGACTCGCTATGTGTTGTTCGTTGGTGCATCAGAGAAGCTTGGATTATGAAGGAACATGGATCTTGGACCCTGCTTTATTCGATTGGGTGGGTCGATGAGTTTCGTCGTGGTTATTGGAAACCTGTGATGCTCTCAAAGAATGGTGAAAGTGTTCTTGTGCAGATAGACTCTAGAAAGTTTGTTTGGCTTGATTTAGAGAAAAAAAGTTGGAAAGAAGTTGAAATTGGTGGTCTACACGACGATGGTTTCAAAGCAACCATTTGCAAGCGGAGCCTTTGCCTTCTTGATGGCGACCCTGTTATTGTTGAGAGGATCAAGAATCCCTTCTCGAGGTTTTCTCCTCCACTACTAGATCAAGAATCCCTTCTCGCCAATACTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

395

Amino Acids

45.52

Weight (kDa)

6.12

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 3 - 41 3.4e-10 F-box domain
F-box-like PF12937 5 - 38 1.2e-08 F-box-like
FBA_3 PF08268 102 - 352 4.5e-18 F-box associated beta propeller domain
FBA_1 PF07734 103 - 336 1.7e-21 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 170
AccB7I CCANNNNNTGG 2 cut(s) 90, 591
AccI GTMKAC 2 cut(s) 354, 1033
AccII CGCG 2 cut(s) 616, 618
AciI CCGC 4 cut(s) 5, 50, 614, 1069
AclI AACGTT 1 cut(s) 422
AclWI GGATC 2 cut(s) 868, 1121
AcsI RAATTY 5 cut(s) 116, 368, 504, 567, 701
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 5 cut(s) 55, 90, 195, 591, 1131
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 8 cut(s) 32, 208, 395, 403, 544, 629, 1022, 1051
AjuI GAANNNNNNNTTGG 4 cut(s) 967, 999, 1008, 1040
AluBI AGCT 3 cut(s) 22, 724, 839
AluI AGCT 3 cut(s) 22, 724, 839
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 3 cut(s) 319, 341, 795
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 2 cut(s) 868, 1121
Ama87I CYCGRG 2 cut(s) 53, 1129
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 1 cut(s) 430
ApoI RAATTY 5 cut(s) 116, 368, 504, 567, 701
Asp700I GAANNNNTTC 3 cut(s) 951, 1124, 1164
AspLEI GCGC 1 cut(s) 618
AspS9I GGNCC 1 cut(s) 867
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 3 cut(s) 706, 785, 956
AsuII TTCGAA 1 cut(s) 656
AvaI CYCGRG 2 cut(s) 53, 1129
AvaII GGWCC 1 cut(s) 867
BaeGI GKGCMC 1 cut(s) 558
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 1 cut(s) 442
BccI CCATC 8 cut(s) 110, 138, 149, 224, 445, 764, 1037, 1082
BceAI ACGGC 1 cut(s) 59
BcnI CCSGG 1 cut(s) 758
BcoDI GTCTC 3 cut(s) 319, 341, 795
BfaI CTAG 3 cut(s) 275, 972, 1151
BfmI CTRYAG 2 cut(s) 177, 237
BfuAI ACCTGC 1 cut(s) 170
BisI GCNGC 2 cut(s) 431, 614
BlsI GCNGC 2 cut(s) 432, 615
Bme1390I CCNGG 1 cut(s) 758
Bme18I GGWCC 1 cut(s) 867
BmeT110I CYCGRG 2 cut(s) 53, 1129
BmgT120I GGNCC 1 cut(s) 867
BmiI GGNNCC 3 cut(s) 47, 869, 1073
BmrFI CCNGG 1 cut(s) 758
BmsI GCATC 3 cut(s) 59, 837, 918
BplI GAGNNNNNCTC 2 cut(s) 1123, 1155
Bpu14I TTCGAA 1 cut(s) 656
BpuMI CCSGG 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 54, 249
BsaWI WCCGGW 1 cut(s) 16
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 90, 195, 591, 1131
Bse1I ACTGG 2 cut(s) 729, 749
Bse3DI GCAATG 1 cut(s) 328
BseDI CCNNGG 2 cut(s) 54, 249
BseGI GGATG 3 cut(s) 102, 141, 775
BseLI CCNNNNNNNGG 5 cut(s) 55, 90, 195, 591, 1131
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 1 cut(s) 1170
BseNI ACTGG 2 cut(s) 729, 749
BseRI GAGGAG 1 cut(s) 1131
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 980
Bsh1236I CGCG 2 cut(s) 616, 618
Bsh1285I CGRYCG 1 cut(s) 354
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 558
BsiHKCI CYCGRG 2 cut(s) 53, 1129
BsiSI CCGG 2 cut(s) 17, 758
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 5 cut(s) 55, 90, 195, 591, 1131
BsmAI GTCTC 3 cut(s) 319, 341, 795
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 551
BsmI GAATGC 2 cut(s) 100, 336
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 2 cut(s) 53, 1129
Bsp119I TTCGAA 1 cut(s) 656
Bsp1286I GDGCHC 1 cut(s) 558
Bsp143I GATC 4 cut(s) 24, 860, 1113, 1153
BspACI CCGC 4 cut(s) 5, 50, 614, 1069
BspCNI CTCAG 1 cut(s) 1171
BspFNI CGCG 2 cut(s) 616, 618
BspLI GGNNCC 3 cut(s) 47, 869, 1073
BspMAI CTGCAG 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 170
BspPI GGATC 2 cut(s) 868, 1121
BspT104I TTCGAA 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 328
BsrI ACTGG 2 cut(s) 729, 749
BssECI CCNNGG 2 cut(s) 54, 249
BssMI GATC 4 cut(s) 24, 860, 1113, 1153
Bst4CI ACNGT 4 cut(s) 264, 352, 461, 662
BstBI TTCGAA 1 cut(s) 656
BstC8I GCNNGC 3 cut(s) 678, 726, 1067
BstDEI CTNAG 1 cut(s) 1179
BstDSI CCRYGG 1 cut(s) 249
BstENI CCTNNNNNAGG 1 cut(s) 1129
BstF5I GGATG 3 cut(s) 102, 141, 775
BstFNI CGCG 2 cut(s) 616, 618
BstHHI GCGC 1 cut(s) 618
BstKTI GATC 4 cut(s) 27, 863, 1116, 1156
BstMAI GTCTC 3 cut(s) 319, 341, 795
BstMBI GATC 4 cut(s) 24, 860, 1113, 1153
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 1 cut(s) 1071
BstNSI RCATGY 1 cut(s) 613
BstSCI CCNGG 1 cut(s) 756
BstSFI CTRYAG 2 cut(s) 177, 237
BstSLI GKGCMC 1 cut(s) 558
BstUI CGCG 2 cut(s) 616, 618
BstV1I GCAGC 1 cut(s) 442
BstX2I RGATCY 1 cut(s) 860
BstYI RGATCY 1 cut(s) 860
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 633
BtsCI GGATG 3 cut(s) 102, 141, 775
BtsIMutI CAGTG 2 cut(s) 736, 742
BveI ACCTGC 1 cut(s) 170
Cac8I GCNNGC 3 cut(s) 678, 726, 1067
CfoI GCGC 1 cut(s) 618
Cfr13I GGNCC 1 cut(s) 867
Csp6I GTAC 1 cut(s) 234
CviAII CATG 5 cut(s) 107, 318, 591, 610, 857
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 1 cut(s) 1179
DpnI GATC 4 cut(s) 26, 862, 1115, 1155
DpnII GATC 4 cut(s) 24, 860, 1113, 1153
EciI GGCGGA 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 319
Eco32I GATATC 1 cut(s) 646
Eco47I GGWCC 1 cut(s) 867
Eco88I CYCGRG 2 cut(s) 53, 1129
EcoNI CCTNNNNNAGG 1 cut(s) 1129
EcoRV GATATC 1 cut(s) 646
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 5 cut(s) 110, 321, 594, 613, 860
FalI AAGNNNNNCTT 4 cut(s) 1109, 1141, 1149, 1181
FaqI GGGAC 1 cut(s) 551
FatI CATG 5 cut(s) 106, 317, 590, 609, 856
FauI CCCGC 1 cut(s) 57
FblI GTMKAC 2 cut(s) 354, 1033
Fnu4HI GCNGC 2 cut(s) 431, 614
FokI GGATG 3 cut(s) 89, 128, 782
Fsp4HI GCNGC 2 cut(s) 431, 614
FspBI CTAG 3 cut(s) 275, 972, 1151
GlaI GCGC 1 cut(s) 617
GluI GCNGC 2 cut(s) 431, 614
HapII CCGG 2 cut(s) 17, 758
HhaI GCGC 1 cut(s) 618
Hin1II CATG 5 cut(s) 110, 321, 594, 613, 860
Hin6I GCGC 1 cut(s) 616
HinP1I GCGC 1 cut(s) 616
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 837
HinfI GANTC 9 cut(s) 255, 292, 382, 471, 482, 803, 968, 1120, 1160
HpaII CCGG 2 cut(s) 17, 758
HphI GGTGA 3 cut(s) 706, 785, 956
Hpy166II GTNNAC 3 cut(s) 355, 556, 1034
Hpy188I TCNGA 3 cut(s) 260, 445, 833
Hpy188III TCNNGA 5 cut(s) 972, 1085, 1117, 1129, 1157
Hpy8I GTNNAC 3 cut(s) 355, 556, 1034
Hpy99I CGWCG 4 cut(s) 197, 497, 909, 1043
HpyAV CCTTC 5 cut(s) 278, 844, 1091, 1135, 1175
HpyCH4III ACNGT 4 cut(s) 264, 352, 461, 662
HpyCH4IV ACGT 4 cut(s) 279, 308, 422, 687
HpyCH4V TGCA 9 cut(s) 179, 321, 336, 457, 518, 556, 828, 961, 1065
HpyF10VI GCNNNNNNNGC 1 cut(s) 1071
HpyF3I CTNAG 1 cut(s) 1179
HpySE526I ACGT 4 cut(s) 279, 308, 422, 687
Hsp92II CATG 5 cut(s) 110, 321, 594, 613, 860
HspAI GCGC 1 cut(s) 616
Kzo9I GATC 4 cut(s) 24, 860, 1113, 1153
LmnI GCTCC 4 cut(s) 19, 51, 143, 1071
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 3 cut(s) 59, 837, 918
MaeI CTAG 3 cut(s) 275, 972, 1151
MaeII ACGT 4 cut(s) 279, 308, 422, 687
MaeIII GTNAC 3 cut(s) 63, 172, 712
MalI GATC 4 cut(s) 26, 862, 1115, 1155
MboI GATC 4 cut(s) 24, 860, 1113, 1153
MflI RGATCY 1 cut(s) 860
MhlI GDGCHC 1 cut(s) 558
MlyI GAGTC 3 cut(s) 286, 797, 962
MmeI TCCRAC 2 cut(s) 609, 989
MroXI GAANNNNTTC 3 cut(s) 951, 1124, 1164
MseI TTAA 2 cut(s) 531, 668
MslI CAYNNNNRTG 1 cut(s) 1041
MspI CCGG 2 cut(s) 17, 758
MspR9I CCNGG 1 cut(s) 758
Mva1269I GAATGC 2 cut(s) 100, 336
MvnI CGCG 2 cut(s) 616, 618
MwoI GCNNNNNNNGC 1 cut(s) 1071
NciI CCSGG 1 cut(s) 758
NdeII GATC 4 cut(s) 24, 860, 1113, 1153
NlaIII CATG 5 cut(s) 110, 321, 594, 613, 860
NlaIV GGNNCC 3 cut(s) 47, 869, 1073
NmuCI GTSAC 1 cut(s) 712
NspI RCATGY 1 cut(s) 613
NspV TTCGAA 1 cut(s) 656
PaeR7I CTCGAG 1 cut(s) 1129
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 2 cut(s) 100, 336
PdmI GAANNNNTTC 3 cut(s) 951, 1124, 1164
PfeI GAWTC 6 cut(s) 255, 382, 471, 482, 1120, 1160
PflFI GACNNNGTC 1 cut(s) 359
PflMI CCANNNNNTGG 2 cut(s) 90, 591
PkrI GCNGC 2 cut(s) 432, 615
PleI GAGTC 3 cut(s) 286, 797, 962
PpsI GAGTC 3 cut(s) 286, 797, 962
Psp1406I AACGTT 1 cut(s) 422
PspN4I GGNNCC 3 cut(s) 47, 869, 1073
PspPI GGNCC 1 cut(s) 867
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 860
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
RseI CAYNNNNRTG 1 cut(s) 1041
SalI GTCGAC 1 cut(s) 353
SaqAI TTAA 2 cut(s) 531, 668
SatI GCNGC 2 cut(s) 431, 614
Sau3AI GATC 4 cut(s) 24, 860, 1113, 1153
Sau96I GGNCC 1 cut(s) 867
SchI GAGTC 3 cut(s) 286, 797, 962
ScrFI CCNGG 1 cut(s) 758
SduI GDGCHC 1 cut(s) 558
SfaNI GCATC 3 cut(s) 59, 837, 918
SfcI CTRYAG 2 cut(s) 177, 237
Sfr274I CTCGAG 1 cut(s) 1129
SfuI TTCGAA 1 cut(s) 656
SinI GGWCC 1 cut(s) 867
SlaI CTCGAG 1 cut(s) 1129
SmiMI CAYNNNNRTG 1 cut(s) 1041
SmlI CTYRAG 1 cut(s) 1129
SmoI CTYRAG 1 cut(s) 1129
SsiI CCGC 4 cut(s) 5, 50, 614, 1069
SspI AATATT 2 cut(s) 220, 547
SspMI CTAG 3 cut(s) 275, 972, 1151
StyD4I CCNGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 264, 352, 461, 662
TaiI ACGT 4 cut(s) 282, 311, 425, 690
TaqI TCGA 6 cut(s) 354, 363, 656, 881, 894, 1130
TauI GCSGC 1 cut(s) 616
TfiI GAWTC 6 cut(s) 255, 382, 471, 482, 1120, 1160
Tru1I TTAA 2 cut(s) 531, 668
Tru9I TTAA 2 cut(s) 531, 668
TscAI CASTG 2 cut(s) 736, 749
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 2 cut(s) 711, 863
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 2 cut(s) 736, 749
Tth111I GACNNNGTC 1 cut(s) 359
Van91I CCANNNNNTGG 2 cut(s) 90, 591
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 1 cut(s) 867
XagI CCTNNNNNAGG 1 cut(s) 1129
XapI RAATTY 5 cut(s) 116, 368, 504, 567, 701
XbaI TCTAGA 1 cut(s) 971
XceI RCATGY 1 cut(s) 613
XhoI CTCGAG 1 cut(s) 1129
XmiI GTMKAC 2 cut(s) 354, 1033
XmnI GAANNNNTTC 3 cut(s) 951, 1124, 1164
XspI CTAG 3 cut(s) 275, 972, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.