Rh6CG375300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000424
Physical Location & Seq
Forward (+)
56146401 .. 56147808
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG375300.1

Sequence Viewer

Length: 1197 bp
ATGTCCACTAAACGCAGCCTTTCAACAACTTCATATTTACCACCGGAAATTATATTTGAGATCCTTTTACATCTCCCACCCAAGGATTTGATACGATTCATGCGTGTTTGTAAAGCTTGGAAGACCACCATCCGCAGCCAAGAGTTTATCAAAGCCCAGCACGATCGCTCCATGAAGACCAACAGTCCTCGCATCATTGTACTTTTACAACGCGGTTCCTACGACCCTTGTAAATATTTCTCTTTATCTGTTTATTTTGACAGAGATGTATTCAGTAATGCTGTGAGGATAAAGCAGCCATTGGAACACCCTGACGAAGAGACCCTCATGCTGGGTTACTGTAACGGTTTGGTTTGCATAAAGTACTCTAACTCAATATCGAATGCTGAGAGTTTTGTGGTATGGAATCCGTCGATTCAAAGGTTCAAGAGAATTCCCTTCACAGCCATCGAGCTGCCAGCTGACACTGGGAACAGAATTCCAAAGTACGGGTTTGGGTATGATTCGACCAATGATGACTATAAACTAGTGAGAGTGGTAGTGTTTACAAATAATGATGGGGATGAAGTACTGAATTCGGAAGTGAAGATTTATAGTCTAAAATCTAACTCATGGAAAAGGATCCAAAGTTTGCCTTGCATTAAGGATTTTCAAATGTATTCAAAAGATCTGTCCTTTTTGAATGGTGCTCTACATTGCCTGATTCGTCATAGGTCAGATAGCAAGATGAAACGAATTCTATCTCTTGATCTTGCAACTGAGAAGTATGACGACTTTCCGGCCCCGCCGGTAGATTACGAGAATCCTACAATATTGATGTTGGAGATCTTGGGAGGATATCTATGTGTTTGTCCTAGACCTTCTCCACCCGCAAAGTGGATCCCCCGAAGTGATGTATGGATTATGAAGGAATATGGAGTGGCAGCATCTTGGACTCGGTTATGTACTATTAAGCAGCAGGATATGCCTCGGATGAGGTACGGATATTGCAAACCTTTGTTATTTTCAAAGACGCGAGAAATGATTCTTTTCAAAAGAAGTTGTGCCAAGCTTTTCTGGTATGATTTAGAGAAAAAGAGGGCAAAACAAATTGACATTTTTGATAAGCCCTCATCCTTTTCGACTGCTATTTGTGTGGGAAACCTTCGTCTCCTTGATGGTGAACCTGTAACTGTTCGTGTCAGTGAAGGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

398

Amino Acids

46.1

Weight (kDa)

9.14

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 11 - 45 1.1e-09 F-box-like
F-box PF00646 13 - 50 2.6e-12 F-box domain
FBA_3 PF08268 78 - 367 1.6e-18 F-box associated beta propeller domain
FBA_1 PF07734 96 - 372 2.9e-26 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 213, 1015
AciI CCGC 4 cut(s) 133, 213, 785, 870
AclWI GGATC 5 cut(s) 55, 616, 629, 874, 887
AcsI RAATTY 4 cut(s) 432, 477, 574, 735
AfaI GTAC 6 cut(s) 201, 365, 488, 570, 946, 980
AfiI CCNNNNNNNGG 4 cut(s) 82, 331, 488, 876
AgsI TTSAA 8 cut(s) 24, 419, 427, 653, 663, 682, 1008, 1033
AhdI GACNNNNNGTC 1 cut(s) 183
AhlI ACTAGT 1 cut(s) 526
AluBI AGCT 4 cut(s) 116, 454, 461, 1051
AluI AGCT 4 cut(s) 116, 454, 461, 1051
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 2 cut(s) 314, 1154
AlwI GGATC 5 cut(s) 55, 616, 629, 874, 887
AoxI GGCC 1 cut(s) 780
ApeKI GCWGC 6 cut(s) 15, 135, 295, 454, 923, 955
ApoI RAATTY 4 cut(s) 432, 477, 574, 735
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
Asp700I GAANNNNTTC 1 cut(s) 1023
AspS9I GGNCC 1 cut(s) 781
AsuHPI GGTGA 1 cut(s) 1172
BamHI GGATCC 2 cut(s) 621, 879
BbsI GAAGAC 2 cut(s) 128, 182
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 6 cut(s) 27, 147, 307, 441, 935, 967
BccI CCATC 4 cut(s) 137, 455, 551, 1151
BcoDI GTCTC 2 cut(s) 314, 1154
BcuI ACTAGT 1 cut(s) 526
BfaI CTAG 2 cut(s) 527, 855
BglII AGATCT 2 cut(s) 667, 825
BisI GCNGC 6 cut(s) 16, 136, 296, 455, 924, 956
BlsI GCNGC 6 cut(s) 17, 137, 297, 456, 925, 957
BmcAI AGTACT 2 cut(s) 365, 570
BmeRI GACNNNNNGTC 1 cut(s) 183
BmgT120I GGNCC 1 cut(s) 781
BmiI GGNNCC 4 cut(s) 217, 623, 783, 881
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 2 cut(s) 201, 935
BmuI ACTGGG 1 cut(s) 477
BpiI GAAGAC 2 cut(s) 128, 182
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 81, 968
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 331, 488, 876
Bse118I RCCGGY 1 cut(s) 787
Bse1I ACTGG 1 cut(s) 472
Bse3DI GCAATG 1 cut(s) 694
BseDI CCNNGG 2 cut(s) 81, 968
BseGI GGATG 4 cut(s) 129, 568, 978, 1112
BseLI CCNNNNNNNGG 4 cut(s) 82, 331, 488, 876
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 2 cut(s) 378, 750
BseNI ACTGG 1 cut(s) 472
BseXI GCAGC 6 cut(s) 27, 147, 307, 441, 935, 967
BseYI CCCAGC 2 cut(s) 156, 331
Bsh1236I CGCG 2 cut(s) 213, 1015
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 1 cut(s) 782
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 3 cut(s) 44, 779, 788
BslI CCNNNNNNNGG 4 cut(s) 82, 331, 488, 876
BsmAI GTCTC 2 cut(s) 314, 1154
BsmBI CGTCTC 1 cut(s) 1154
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 1 cut(s) 782
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
BspACI CCGC 4 cut(s) 133, 213, 785, 870
BspANI GGCC 1 cut(s) 782
BspCNI CTCAG 2 cut(s) 379, 751
BspFNI CGCG 2 cut(s) 213, 1015
BspLI GGNNCC 4 cut(s) 217, 623, 783, 881
BspPI GGATC 5 cut(s) 55, 616, 629, 874, 887
BspTNI GGTCTC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 694
BsrFI RCCGGY 1 cut(s) 787
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 787
BssECI CCNNGG 2 cut(s) 81, 968
BssMI GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 4 cut(s) 185, 341, 347, 1174
Bst6I CTCTTC 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 964
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 387, 759
BstF5I GGATG 4 cut(s) 129, 568, 978, 1112
BstFNI CGCG 2 cut(s) 213, 1015
BstKTI GATC 7 cut(s) 63, 166, 624, 670, 751, 828, 882
BstMAI GTCTC 2 cut(s) 314, 1154
BstMBI GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 964
BstUI CGCG 2 cut(s) 213, 1015
BstV1I GCAGC 6 cut(s) 27, 147, 307, 441, 935, 967
BstV2I GAAGAC 2 cut(s) 128, 182
BstX2I RGATCY 5 cut(s) 60, 621, 667, 825, 879
BstYI RGATCY 5 cut(s) 60, 621, 667, 825, 879
BsuRI GGCC 1 cut(s) 782
BtsCI GGATG 4 cut(s) 129, 568, 978, 1112
BtsIMutI CAGTG 2 cut(s) 465, 1189
Cac8I GCNNGC 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 787
Cfr13I GGNCC 1 cut(s) 781
CseI GACGC 1 cut(s) 1021
Csp6I GTAC 6 cut(s) 200, 364, 487, 569, 945, 979
CviAII CATG 4 cut(s) 100, 172, 328, 612
CviQI GTAC 6 cut(s) 200, 364, 487, 569, 945, 979
DdeI CTNAG 2 cut(s) 387, 759
DpnI GATC 7 cut(s) 62, 165, 623, 669, 750, 827, 881
DpnII GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
DriI GACNNNNNGTC 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 312
Eam1105I GACNNNNNGTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 314
Eco32I GATATC 1 cut(s) 839
EcoRI GAATTC 4 cut(s) 432, 477, 574, 735
EcoRV GATATC 1 cut(s) 839
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 1154
FaeI CATG 4 cut(s) 103, 175, 331, 615
FalI AAGNNNNNCTT 2 cut(s) 619, 651
FatI CATG 4 cut(s) 99, 171, 327, 611
FauI CCCGC 2 cut(s) 792, 877
Fnu4HI GCNGC 6 cut(s) 16, 136, 296, 455, 924, 956
FokI GGATG 4 cut(s) 116, 575, 985, 1099
Fsp4HI GCNGC 6 cut(s) 16, 136, 296, 455, 924, 956
FspBI CTAG 2 cut(s) 527, 855
GluI GCNGC 6 cut(s) 16, 136, 296, 455, 924, 956
GsaI CCCAGC 2 cut(s) 160, 335
HaeIII GGCC 1 cut(s) 782
HapII CCGG 3 cut(s) 44, 779, 788
HgaI GACGC 1 cut(s) 1021
Hin1II CATG 4 cut(s) 103, 175, 331, 615
HindIII AAGCTT 2 cut(s) 114, 1049
HinfI GANTC 8 cut(s) 96, 406, 415, 503, 703, 802, 934, 1024
HpaII CCGG 3 cut(s) 44, 779, 788
HphI GGTGA 1 cut(s) 1172
Hpy166II GTNNAC 3 cut(s) 6, 546, 1163
Hpy188I TCNGA 3 cut(s) 580, 718, 972
Hpy188III TCNNGA 2 cut(s) 427, 746
Hpy8I GTNNAC 3 cut(s) 6, 546, 1163
Hpy99I CGWCG 1 cut(s) 415
HpyAV CCTTC 5 cut(s) 448, 870, 901, 1154, 1181
HpyCH4III ACNGT 4 cut(s) 185, 341, 347, 1174
HpyCH4V TGCA 4 cut(s) 357, 639, 755, 990
HpyF10VI GCNNNNNNNGC 1 cut(s) 964
HpyF3I CTNAG 2 cut(s) 387, 759
Hsp92II CATG 4 cut(s) 103, 175, 331, 615
Kzo9I GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 6 cut(s) 27, 147, 307, 441, 935, 967
LweI GCATC 2 cut(s) 201, 935
MaeI CTAG 2 cut(s) 527, 855
MaeIII GTNAC 3 cut(s) 335, 341, 1168
MalI GATC 7 cut(s) 62, 165, 623, 669, 750, 827, 881
MboI GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
MboII GAAGA 4 cut(s) 133, 187, 329, 598
MflI RGATCY 5 cut(s) 60, 621, 667, 825, 879
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 6 cut(s) 48, 432, 477, 574, 735, 1089
MlyI GAGTC 1 cut(s) 928
MmeI TCCRAC 1 cut(s) 801
MnlI CCTC 8 cut(s) 198, 279, 335, 827, 969, 978, 1071, 1120
MroXI GAANNNNTTC 1 cut(s) 1023
MseI TTAA 2 cut(s) 642, 951
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 3 cut(s) 44, 779, 788
Mva1269I GAATGC 1 cut(s) 388
MvnI CGCG 2 cut(s) 213, 1015
MwoI GCNNNNNNNGC 1 cut(s) 964
NdeII GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
NlaIII CATG 4 cut(s) 103, 175, 331, 615
NlaIV GGNNCC 4 cut(s) 217, 623, 783, 881
PcsI WCGNNNNNNNCGW 1 cut(s) 100
PctI GAATGC 1 cut(s) 388
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 7 cut(s) 96, 406, 415, 503, 703, 802, 1024
PkrI GCNGC 6 cut(s) 17, 137, 297, 456, 925, 957
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 1 cut(s) 928
PpsI GAGTC 1 cut(s) 928
PspFI CCCAGC 2 cut(s) 156, 331
PspN4I GGNNCC 4 cut(s) 217, 623, 783, 881
PspPI GGNCC 1 cut(s) 781
PsuI RGATCY 5 cut(s) 60, 621, 667, 825, 879
PvuI CGATCG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 6 cut(s) 201, 365, 488, 570, 946, 980
RsaNI GTAC 6 cut(s) 200, 364, 487, 569, 945, 979
SaqAI TTAA 2 cut(s) 642, 951
SatI GCNGC 6 cut(s) 16, 136, 296, 455, 924, 956
Sau3AI GATC 7 cut(s) 60, 163, 621, 667, 748, 825, 879
Sau96I GGNCC 1 cut(s) 781
ScaI AGTACT 2 cut(s) 365, 570
SchI GAGTC 1 cut(s) 928
SduI GDGCHC 1 cut(s) 691
SfaNI GCATC 2 cut(s) 201, 935
SpeI ACTAGT 1 cut(s) 526
Sse9I AATT 6 cut(s) 48, 432, 477, 574, 735, 1089
SsiI CCGC 4 cut(s) 133, 213, 785, 870
SspI AATATT 2 cut(s) 236, 813
SspMI CTAG 2 cut(s) 527, 855
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 4 cut(s) 185, 341, 347, 1174
TaqI TCGA 5 cut(s) 380, 413, 450, 506, 1121
TasI AATT 6 cut(s) 48, 432, 477, 574, 735, 1089
TatI WGTACW 4 cut(s) 199, 363, 568, 944
TfiI GAWTC 7 cut(s) 96, 406, 415, 503, 703, 802, 1024
Tru1I TTAA 2 cut(s) 642, 951
Tru9I TTAA 2 cut(s) 642, 951
TscAI CASTG 2 cut(s) 472, 1189
TseI GCWGC 6 cut(s) 15, 135, 295, 454, 923, 955
TspDTI ATGAA 6 cut(s) 21, 88, 188, 579, 743, 920
TspGWI ACGGA 2 cut(s) 399, 996
TspRI CASTG 2 cut(s) 472, 1189
XapI RAATTY 4 cut(s) 432, 477, 574, 735
XcmI CCANNNNNNNNNTGG 1 cut(s) 873
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 2 cut(s) 527, 855
ZrmI AGTACT 2 cut(s) 365, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.