pycom05g27240

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000424
Physical Location & Seq
Forward (+)
28270015 .. 28270936
922 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27240.1

Sequence Viewer

Length: 882 bp
ATGCCACCATCCATGATCGAAAGTTCATCGATGCCCATCAGCAACGGAGCCGAATTCGGTCTTCCCAACGCTGCGGAGAACGACTTGTGTTCATTGGCGTTCTACGATAATGATACGTTGGGGACCATTGAGAAACTGGAAGCGCCGCTTAAACGCCGTGGTACTTGCACTCACATATTGGGCTACTCTAACGGTCTTGTTTGCATCCGCAACAGTAAACATAGTAGCGATGAAGGCGATTTTGCCATTTGGAATCCATCAATTCAAAAGTTCAAGAAGATATCCTCTACAAGCTTTGAGCAGCCAACAGATTTGACATTAGGACGTCCCTGTTATGGTTTCGGGTATGATTTCGCTAACGATGACTATAAATTTGTGAAAATTGTGGAATTTCTTGATGGGGACGGGGTTATTACGGGCGCCCAGGTGAAGGTTTATAGCTTAAAATCCTACTCCTGGAAACGGATCCAAAGCATGTCTGCCAATGGTTTTGGAGTTGATTCAAAGAATATCCGGTTTCTGAAAGGTGCTCTAAGTTGGCTAACAAGAAACGCAGGTGACAACCGTTACAAAATTCTGTCTCTTGATCTTGCCACTGAGCAATGTTGTGAGTTTCCCACCCCGTTTAATGGGGATGACAATTCTAGACTGTTTTTGGACGTTTTAGGAAGCTTTTTATGTATTTGTGTTGGTCATTTTTATATCCGAAAGGATTTTTGGATTATGAAAAAATTTGGGGAGGCAGAATCTTGGTCCCTAATTTATTCTGTTGATGTGGAAACCATACGTACATCGCTTGGCTATTTCAAACCTATGGCTTTTTCAAAGAATGGTGAAATGGTTCTTTTTACGGACAACTATGATAAGTCTTTTGGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

294

Amino Acids

32.93

Weight (kDa)

5.52

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 46 - 291 4.3e-12 F-box associated beta propeller domain
FBA_1 PF07734 60 - 272 2.6e-19 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 545
AatII GACGTC 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 419
AciI CCGC 3 cut(s) 74, 146, 208
AclWI GGATC 2 cut(s) 460, 473
AcsI RAATTY 5 cut(s) 53, 371, 389, 573, 731
AcyI GRCGYC 2 cut(s) 325, 420
AfaI GTAC 2 cut(s) 163, 790
AfiI CCNNNNNNNGG 5 cut(s) 335, 430, 456, 462, 629
AgsI TTSAA 5 cut(s) 266, 274, 504, 808, 825
AjnI CCWGG 2 cut(s) 423, 455
AjuI GAANNNNNNNTTGG 2 cut(s) 700, 732
AluBI AGCT 3 cut(s) 294, 441, 672
AluI AGCT 3 cut(s) 294, 441, 672
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 1 cut(s) 585
AlwI GGATC 2 cut(s) 460, 473
ApeKI GCWGC 2 cut(s) 71, 301
ApoI RAATTY 5 cut(s) 53, 371, 389, 573, 731
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
Asp700I GAANNNNTTC 1 cut(s) 840
AspLEI GCGC 2 cut(s) 145, 422
AspS9I GGNCC 2 cut(s) 123, 753
AsuHPI GGTGA 3 cut(s) 439, 569, 845
AvaII GGWCC 2 cut(s) 123, 753
BamHI GGATCC 1 cut(s) 465
BanI GGYRCC 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 2 cut(s) 58, 313
BccI CCATC 4 cut(s) 16, 44, 265, 392
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 2 cut(s) 425, 457
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 1 cut(s) 645
BfoI RGCGCY 2 cut(s) 146, 423
BfuAI ACCTGC 1 cut(s) 545
BisI GCNGC 3 cut(s) 72, 146, 302
BlsI GCNGC 3 cut(s) 73, 147, 303
Bme1390I CCNGG 2 cut(s) 425, 457
Bme18I GGWCC 2 cut(s) 123, 753
BmgT120I GGNCC 2 cut(s) 123, 753
BmiI GGNNCC 5 cut(s) 49, 124, 421, 467, 755
BmrFI CCNGG 2 cut(s) 425, 457
BmsI GCATC 2 cut(s) 21, 213
BpiI GAAGAC 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 29
BsaAI YACGTR 1 cut(s) 788
BsaBI GATNNNNATC 1 cut(s) 35
BsaHI GRCGYC 2 cut(s) 325, 420
BsaJI CCNNGG 2 cut(s) 157, 423
BsaWI WCCGGW 1 cut(s) 513
Bsc4I CCNNNNNNNGG 5 cut(s) 335, 430, 456, 462, 629
Bse1I ACTGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 608
Bse8I GATNNNNATC 1 cut(s) 35
BseBI CCWGG 2 cut(s) 425, 457
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 157, 423
BseGI GGATG 3 cut(s) 8, 204, 640
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 5 cut(s) 335, 430, 456, 462, 629
BseMI GCAATG 1 cut(s) 608
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 1 cut(s) 141
BseXI GCAGC 2 cut(s) 58, 313
BshNI GGYRCC 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 532
BsiSI CCGG 1 cut(s) 514
BslFI GGGAC 4 cut(s) 136, 312, 416, 739
BslI CCNNNNNNNGG 5 cut(s) 335, 430, 456, 462, 629
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 4 cut(s) 136, 312, 416, 739
Bsp1286I GDGCHC 1 cut(s) 532
Bsp143I GATC 3 cut(s) 15, 465, 586
BspACI CCGC 3 cut(s) 74, 146, 208
BspCNI CTCAG 1 cut(s) 589
BspDI ATCGAT 1 cut(s) 29
BspLI GGNNCC 5 cut(s) 49, 124, 421, 467, 755
BspMI ACCTGC 1 cut(s) 545
BspPI GGATC 2 cut(s) 460, 473
BspT107I GGYRCC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 608
BsrI ACTGG 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 157, 423
BssMI GATC 3 cut(s) 15, 465, 586
BssNI GRCGYC 2 cut(s) 325, 420
Bst2UI CCWGG 2 cut(s) 425, 457
Bst4CI ACNGT 4 cut(s) 194, 215, 566, 651
BstACI GRCGYC 2 cut(s) 325, 420
BstBAI YACGTR 1 cut(s) 788
BstDEI CTNAG 2 cut(s) 533, 597
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 3 cut(s) 8, 204, 640
BstH2I RGCGCY 2 cut(s) 146, 423
BstHHI GCGC 2 cut(s) 145, 422
BstKTI GATC 3 cut(s) 18, 468, 589
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 3 cut(s) 15, 465, 586
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstNI CCWGG 2 cut(s) 425, 457
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 423, 455
BstSNI TACGTA 1 cut(s) 788
BstV1I GCAGC 2 cut(s) 58, 313
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
Bsu15I ATCGAT 1 cut(s) 29
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 157
BtgZI GCGATG 2 cut(s) 243, 777
BtsCI GGATG 3 cut(s) 8, 204, 640
BtsIMutI CAGTG 1 cut(s) 594
BveI ACCTGC 1 cut(s) 545
CfoI GCGC 2 cut(s) 145, 422
Cfr13I GGNCC 2 cut(s) 123, 753
ClaI ATCGAT 1 cut(s) 29
Csp6I GTAC 2 cut(s) 162, 789
CviAII CATG 2 cut(s) 13, 475
CviQI GTAC 2 cut(s) 162, 789
DdeI CTNAG 2 cut(s) 533, 597
DinI GGCGCC 1 cut(s) 421
DpnI GATC 3 cut(s) 17, 467, 588
DpnII GATC 3 cut(s) 15, 465, 586
Eco105I TACGTA 1 cut(s) 788
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 2 cut(s) 123, 753
EcoRI GAATTC 1 cut(s) 53
EcoRII CCWGG 2 cut(s) 423, 455
EcoRV GATATC 1 cut(s) 282
EgeI GGCGCC 1 cut(s) 421
EheI GGCGCC 1 cut(s) 421
FaeI CATG 2 cut(s) 16, 478
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FaqI GGGAC 4 cut(s) 136, 312, 416, 739
FatI CATG 2 cut(s) 12, 474
Fnu4HI GCNGC 3 cut(s) 72, 146, 302
FokI GGATG 2 cut(s) 191, 647
Fsp4HI GCNGC 3 cut(s) 72, 146, 302
FspBI CTAG 1 cut(s) 645
GlaI GCGC 2 cut(s) 144, 421
GluI GCNGC 3 cut(s) 72, 146, 302
HaeII RGCGCY 2 cut(s) 146, 423
HapII CCGG 1 cut(s) 514
HhaI GCGC 2 cut(s) 145, 422
Hin1I GRCGYC 2 cut(s) 325, 420
Hin1II CATG 2 cut(s) 16, 478
Hin6I GCGC 2 cut(s) 143, 420
HinP1I GCGC 2 cut(s) 143, 420
HindIII AAGCTT 2 cut(s) 292, 670
HinfI GANTC 3 cut(s) 253, 500, 746
HpaII CCGG 1 cut(s) 514
HphI GGTGA 3 cut(s) 439, 569, 845
Hpy166II GTNNAC 1 cut(s) 218
Hpy188I TCNGA 2 cut(s) 522, 707
Hpy188III TCNNGA 4 cut(s) 274, 395, 584, 645
Hpy8I GTNNAC 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 227, 424
HpyCH4III ACNGT 4 cut(s) 194, 215, 566, 651
HpyCH4IV ACGT 4 cut(s) 116, 325, 660, 787
HpyCH4V TGCA 2 cut(s) 168, 204
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpyF3I CTNAG 2 cut(s) 533, 597
HpySE526I ACGT 4 cut(s) 116, 325, 660, 787
Hsp92I GRCGYC 2 cut(s) 325, 420
Hsp92II CATG 2 cut(s) 16, 478
HspAI GCGC 2 cut(s) 143, 420
KasI GGCGCC 1 cut(s) 419
Kzo9I GATC 3 cut(s) 15, 465, 586
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 8 cut(s) 122, 343, 410, 437, 442, 469, 527, 540
Lsp1109I GCAGC 2 cut(s) 58, 313
LweI GCATC 2 cut(s) 21, 213
MaeI CTAG 1 cut(s) 645
MaeII ACGT 4 cut(s) 116, 325, 660, 787
MaeIII GTNAC 2 cut(s) 557, 566
MalI GATC 3 cut(s) 17, 467, 588
MboI GATC 3 cut(s) 15, 465, 586
MboII GAAGA 2 cut(s) 53, 289
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 9 cut(s) 53, 261, 371, 381, 389, 573, 640, 731, 759
Mly113I GGCGCC 1 cut(s) 420
MnlI CCTC 2 cut(s) 295, 733
MroXI GAANNNNTTC 1 cut(s) 840
MseI TTAA 3 cut(s) 150, 443, 627
MspI CCGG 1 cut(s) 514
MspR9I CCNGG 2 cut(s) 425, 457
MvaI CCWGG 2 cut(s) 425, 457
MwoI GCNNNNNNNGC 1 cut(s) 234
NarI GGCGCC 1 cut(s) 420
NdeII GATC 3 cut(s) 15, 465, 586
NlaIII CATG 2 cut(s) 16, 478
NlaIV GGNNCC 5 cut(s) 49, 124, 421, 467, 755
NmuCI GTSAC 1 cut(s) 557
NspI RCATGY 1 cut(s) 478
PaqCI CACCTGC 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 3 cut(s) 253, 500, 746
PfoI TCCNGGA 1 cut(s) 455
PkrI GCNGC 3 cut(s) 73, 147, 303
PluTI GGCGCC 1 cut(s) 423
Ppu21I YACGTR 1 cut(s) 788
Psp6I CCWGG 2 cut(s) 423, 455
PspGI CCWGG 2 cut(s) 423, 455
PspN4I GGNNCC 5 cut(s) 49, 124, 421, 467, 755
PspPI GGNCC 2 cut(s) 123, 753
PsuI RGATCY 1 cut(s) 465
RsaI GTAC 2 cut(s) 163, 790
RsaNI GTAC 2 cut(s) 162, 789
SaqAI TTAA 3 cut(s) 150, 443, 627
SatI GCNGC 3 cut(s) 72, 146, 302
Sau3AI GATC 3 cut(s) 15, 465, 586
Sau96I GGNCC 2 cut(s) 123, 753
ScrFI CCNGG 2 cut(s) 425, 457
SduI GDGCHC 1 cut(s) 532
SfaNI GCATC 2 cut(s) 21, 213
SfoI GGCGCC 1 cut(s) 421
SinI GGWCC 2 cut(s) 123, 753
SnaBI TACGTA 1 cut(s) 788
Sse9I AATT 9 cut(s) 53, 261, 371, 381, 389, 573, 640, 731, 759
SsiI CCGC 3 cut(s) 74, 146, 208
SspDI GGCGCC 1 cut(s) 419
SspMI CTAG 1 cut(s) 645
StyD4I CCNGG 2 cut(s) 423, 455
TaaI ACNGT 4 cut(s) 194, 215, 566, 651
TaiI ACGT 4 cut(s) 119, 328, 663, 790
TaqI TCGA 2 cut(s) 18, 29
TaqII GACCGA 1 cut(s) 47
TasI AATT 9 cut(s) 53, 261, 371, 381, 389, 573, 640, 731, 759
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 3 cut(s) 253, 500, 746
Tru1I TTAA 3 cut(s) 150, 443, 627
Tru9I TTAA 3 cut(s) 150, 443, 627
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 2 cut(s) 71, 301
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 4 cut(s) 15, 81, 246, 740
TspGWI ACGGA 3 cut(s) 60, 478, 866
TspRI CASTG 1 cut(s) 601
VpaK11BI GGWCC 2 cut(s) 123, 753
XapI RAATTY 5 cut(s) 53, 371, 389, 573, 731
XbaI TCTAGA 1 cut(s) 644
XceI RCATGY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 1 cut(s) 645
ZraI GACGTC 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.