pycom02g22350

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000424
Physical Location & Seq
Forward (+)
20485901 .. 20486347
447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22350.1

Sequence Viewer

Length: 447 bp
ATGCGTAAGAATCTTGCTTTGTGGAACCCGTCGATTCAAAAGTTCAAGAAGATTCCTCTCCCAACCTTTGAGATTGAGCAGCAGGCATCCTCATACGGGGTCCCTGAATATGGATTCGGGTACGATTCAGCTAATCACGATTATAAAATTTTGGCAATTTTGAACTTTAATCGGAGGGATGATGCTACCATGAGTTCTCAAGTCAGTCTTTATAGCCTAAAATTCAACTCATGGAAAAGGATCCCAAAATTGCCTTGCGATGGTTTCTATGTGCAGACGGGCGATGTGGTGTTTTTGAACGGTGCTATGAGTTGTTTGATGCGGAAATCAGATTCATATAAAAATCGATGCAAAATTGTAGCCCTTGATCGATCCTGGTTTTTCTTGCCAGTGAGAAGTATATGGAGTTTGGCACCCCGGTGGGGGATGAGGATGACAATTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

149

Amino Acids

17.23

Weight (kDa)

9.74

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 3 - 123 3.5e-12 F-box associated beta propeller domain
FBA_3 PF08268 3 - 105 5.4e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 412
AciI CCGC 1 cut(s) 322
AclWI GGATC 3 cut(s) 235, 248, 366
AcsI RAATTY 2 cut(s) 147, 221
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 5 cut(s) 96, 110, 260, 422, 423
AgsI TTSAA 5 cut(s) 38, 46, 163, 226, 298
AjnI CCWGG 1 cut(s) 374
AleI CACNNNNGTG 1 cut(s) 418
AluBI AGCT 1 cut(s) 131
AluI AGCT 1 cut(s) 131
AlwI GGATC 3 cut(s) 235, 248, 366
ApeKI GCWGC 1 cut(s) 79
ApoI RAATTY 2 cut(s) 147, 221
AspS9I GGNCC 1 cut(s) 100
AsuC2I CCSGG 1 cut(s) 418
AvaII GGWCC 1 cut(s) 100
BamHI GGATCC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 412
BbvI GCAGC 1 cut(s) 91
BccI CCATC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 376
BcnI CCSGG 1 cut(s) 418
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
Bme1390I CCNGG 2 cut(s) 376, 418
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 5 cut(s) 26, 101, 102, 242, 414
BmrFI CCNGG 2 cut(s) 376, 418
BmsI GCATC 4 cut(s) 95, 172, 309, 338
BpuEI CTTGAG 1 cut(s) 183
BpuMI CCSGG 1 cut(s) 418
Bsa29I ATCGAT 2 cut(s) 346, 370
BsaJI CCNNGG 1 cut(s) 416
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 110, 260, 422, 423
Bse1I ACTGG 1 cut(s) 389
BseBI CCWGG 1 cut(s) 376
BseCI ATCGAT 2 cut(s) 346, 370
BseDI CCNNGG 1 cut(s) 416
BseGI GGATG 4 cut(s) 86, 184, 432, 438
BseLI CCNNNNNNNGG 5 cut(s) 96, 110, 260, 422, 423
BseNI ACTGG 1 cut(s) 389
BseXI GCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 293
BshNI GGYRCC 1 cut(s) 412
BshVI ATCGAT 2 cut(s) 346, 370
BsiSI CCGG 1 cut(s) 418
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 5 cut(s) 96, 110, 260, 422, 423
BsmFI GGGAC 1 cut(s) 86
Bsp143I GATC 3 cut(s) 240, 367, 371
BspACI CCGC 1 cut(s) 322
BspDI ATCGAT 2 cut(s) 346, 370
BspLI GGNNCC 5 cut(s) 26, 101, 102, 242, 414
BspPI GGATC 3 cut(s) 235, 248, 366
BspT107I GGYRCC 1 cut(s) 412
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 1 cut(s) 416
BssMI GATC 3 cut(s) 240, 367, 371
Bst2UI CCWGG 1 cut(s) 376
Bst4CI ACNGT 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 84
BstF5I GGATG 4 cut(s) 86, 184, 432, 438
BstKTI GATC 3 cut(s) 243, 370, 374
BstMBI GATC 3 cut(s) 240, 367, 371
BstNI CCWGG 1 cut(s) 376
BstSCI CCNGG 2 cut(s) 374, 416
BstV1I GCAGC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
Bsu15I ATCGAT 2 cut(s) 346, 370
BsuTUI ATCGAT 2 cut(s) 346, 370
BtgZI GCGATG 2 cut(s) 273, 297
BtsCI GGATG 4 cut(s) 86, 184, 432, 438
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 100
ClaI ATCGAT 2 cut(s) 346, 370
Csp6I GTAC 1 cut(s) 121
CviAII CATG 2 cut(s) 190, 231
CviJI RGCY 3 cut(s) 131, 216, 362
CviKI_1 RGCY 3 cut(s) 131, 216, 362
CviQI GTAC 1 cut(s) 121
DpnI GATC 3 cut(s) 242, 369, 373
DpnII GATC 3 cut(s) 240, 367, 371
Eco47I GGWCC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 374
FaeI CATG 2 cut(s) 193, 234
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 1 cut(s) 86
FatI CATG 2 cut(s) 189, 230
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 3 cut(s) 73, 191, 439
Fsp4HI GCNGC 1 cut(s) 80
GluI GCNGC 1 cut(s) 80
HapII CCGG 1 cut(s) 418
Hin1II CATG 2 cut(s) 193, 234
HinfI GANTC 6 cut(s) 10, 34, 52, 114, 125, 332
HpaII CCGG 1 cut(s) 418
Hpy188I TCNGA 2 cut(s) 174, 331
Hpy188III TCNNGA 2 cut(s) 46, 137
Hpy99I CGWCG 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 274, 351
Hsp92II CATG 2 cut(s) 193, 234
KflI GGGWCCC 1 cut(s) 100
Kzo9I GATC 3 cut(s) 240, 367, 371
LpnPI CCDG 6 cut(s) 68, 117, 361, 388, 402, 431
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 4 cut(s) 95, 172, 309, 338
MalI GATC 3 cut(s) 242, 369, 373
MboI GATC 3 cut(s) 240, 367, 371
MboII GAAGA 1 cut(s) 61
MflI RGATCY 1 cut(s) 240
MluCI AATT 6 cut(s) 147, 156, 221, 248, 354, 438
MnlI CCTC 4 cut(s) 66, 100, 168, 423
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 1 cut(s) 418
MspI CCGG 1 cut(s) 418
MspR9I CCNGG 2 cut(s) 376, 418
MvaI CCWGG 1 cut(s) 376
NciI CCSGG 1 cut(s) 418
NdeII GATC 3 cut(s) 240, 367, 371
NlaIII CATG 2 cut(s) 193, 234
NlaIV GGNNCC 5 cut(s) 26, 101, 102, 242, 414
OliI CACNNNNGTG 1 cut(s) 418
PfeI GAWTC 6 cut(s) 10, 34, 52, 114, 125, 332
PkrI GCNGC 1 cut(s) 81
PpuMI RGGWCCY 1 cut(s) 100
PsiI TTATAA 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 374
PspGI CCWGG 1 cut(s) 374
PspN4I GGNNCC 5 cut(s) 26, 101, 102, 242, 414
PspPI GGNCC 1 cut(s) 100
PspPPI RGGWCCY 1 cut(s) 100
PsuI RGATCY 1 cut(s) 240
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 418
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 3 cut(s) 240, 367, 371
Sau96I GGNCC 1 cut(s) 100
ScrFI CCNGG 2 cut(s) 376, 418
SetI ASST 2 cut(s) 68, 133
SfaNI GCATC 4 cut(s) 95, 172, 309, 338
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 418
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 6 cut(s) 147, 156, 221, 248, 354, 438
SsiI CCGC 1 cut(s) 322
StyD4I CCNGG 2 cut(s) 374, 416
TaaI ACNGT 1 cut(s) 302
TaqI TCGA 3 cut(s) 32, 346, 370
TasI AATT 6 cut(s) 147, 156, 221, 248, 354, 438
TfiI GAWTC 6 cut(s) 10, 34, 52, 114, 125, 332
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 1 cut(s) 396
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 396
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 2 cut(s) 147, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.