MD05G1183700.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000424
Physical Location & Seq
Reverse (-)
31230848 .. 31232224
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1183700.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGTCAGACCTCCCATTGGACCTGCTCTTTAAGATCCTTTTACTTCTGCAGCCCAAGGATATGATCCGATGCATGTGTGTTTCCAAAGCTTGGAATTCTTCTATCCGCAACCAACGGTTCATCACCATCAAATCCAACTTTTGTCGCACCATTATACTTAATCTGTATGATCCCATGCATTTCTTGTCGTTGCCTTTGTACGACAATGATACGTTGGGGACTGGGATGAAAATTGCACGGCCGCCATTGGAACAATCTTGTGATAGCATATTGGGCTACTCTAATGGCTTGTTATGCATTGAGCACACCTATGACAACAATAGAGCTTTTGCTTTGTGGAACCCGTCCATTCAAAAGTTCAAGATGATTCCTTGTACAACCACCTCTGAGCCGCACCTCCAGCAGCCATCGGAGATGAACGACAAACTGAAGAAGCAAGGGCACTATGGATTCGGGTATGATTCAACTAATGATGACTATAAACTTGTGGAAGTTGTGGAGTTTTGTAGTATAGATAAACGGCGTTCCACTGGATCTGTTTGTGGAAATTGTGTATACGAGGATATGGTTGTTGCTAGTTATGAAGTGAATGTTCATAACCTGAAATCCAACGCGTGGAAAAGGATCCAAAGCATGCCTCCTCGCGATGGCTTTCGACTTCCTCCATTACAATCAGTGGCCGTGTGTTTGAACAGTGCCTTAAGTTGGAAGATGGATTGCCATTTAGATGACGATCGTCCAGGCATGATTCTAACTCTTGATCTTGCCAGTGAGAAATATCGCGAGTTTCCCATCCCAATCCGTGGGGACGAGGATGAGATTTCTTTGGGTTTGGTGGGAGGCTATTTATGTATTTGTCATCGTAATTTTGAGGTTTCAGACTTTCAAATTGATGTTTGGATTATGAAGGAATATGGAGTGACGGGATCTTGGAACCTGCTTTGTTCCGTTGAGAAGTCCGTTGACTCCCACATCAAACCTTTGGTATTTTCCAAGAATGGCGACATGGTACTTTTTGAGATGGACTGTGAGCAGCTTTTTTGGTTTGATTTAGAGAAAAAGAGTGCGAAAGTTGAATTTTGTCGTGAGCCTGGTGACTTTGAGGTAAATGTTTGTGAAGGTGGGGGCTCGGGGAGCGTTTGTGTTCTTCTTGATGGTGATCCTGTAATTGTTGGGAGGCAGCAACAACTGTGTTCGACCTCTGAGAAAGAAGAAACTTTCAATATTGAAGAAGGTGTTCCATCACATATGGTACTCAAAGCAACAGAAAATCCGAGTTTAGTGCTCCTTTCTTTCTTTAGACTAGGCTTTTTTATCTTTGTAATATTGTGTATTTCTTTTTCTTTTTTTGTATCATTGATCTATGGTAACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

459

Amino Acids

51.99

Weight (kDa)

5.08

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 1 - 40 3.7e-10 F-box domain
F-box-like PF12937 1 - 37 4e-08 F-box-like
FBA_3 PF08268 57 - 357 1.9e-13 F-box associated beta propeller domain
FBA_1 PF07734 93 - 358 4.7e-19 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 30, 945
AccB7I CCANNNNNTGG 3 cut(s) 90, 615, 803
AccI GTMKAC 1 cut(s) 555
AccII CGCG 3 cut(s) 614, 645, 783
AciI CCGC 3 cut(s) 106, 242, 392
AclWI GGATC 8 cut(s) 28, 58, 164, 541, 619, 632, 934, 1154
AcoI YGGCCR 2 cut(s) 239, 678
AcsI RAATTY 2 cut(s) 94, 1076
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 4 cut(s) 200, 376, 1011, 1254
AfiI CCNNNNNNNGG 6 cut(s) 16, 90, 615, 647, 705, 803
AflII CTTAAG 1 cut(s) 700
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 8 cut(s) 353, 361, 465, 691, 887, 1076, 1222, 1229
AjnI CCWGG 2 cut(s) 739, 1090
AluBI AGCT 4 cut(s) 89, 326, 1036, 1374
AluI AGCT 4 cut(s) 89, 326, 1036, 1374
Alw21I GWGCWC 2 cut(s) 306, 1287
AlwI GGATC 8 cut(s) 28, 58, 164, 541, 619, 632, 934, 1154
Ama87I CYCGRG 1 cut(s) 1129
AoxI GGCC 2 cut(s) 239, 678
ApeKI GCWGC 4 cut(s) 49, 403, 1033, 1180
ApoI RAATTY 2 cut(s) 94, 1076
Asp700I GAANNNNTTC 1 cut(s) 1236
AspS9I GGNCC 1 cut(s) 19
AsuHPI GGTGA 3 cut(s) 115, 1106, 1169
AvaI CYCGRG 1 cut(s) 1129
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 444
BamHI GGATCC 1 cut(s) 624
BanII GRGCYC 1 cut(s) 1130
Bbv12I GWGCWC 2 cut(s) 306, 1287
BbvI GCAGC 4 cut(s) 61, 415, 1045, 1192
BccI CCATC 8 cut(s) 134, 415, 641, 706, 800, 1015, 1148, 1249
BceAI ACGGC 3 cut(s) 254, 536, 665
BcgI CGANNNNNNTGC 2 cut(s) 1264, 1298
BciT130I CCWGG 2 cut(s) 741, 1092
BfaI CTAG 3 cut(s) 576, 1304, 1375
BfmI CTRYAG 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 700
BfuAI ACCTGC 2 cut(s) 30, 945
BisI GCNGC 6 cut(s) 50, 242, 392, 404, 1034, 1181
BlsI GCNGC 6 cut(s) 51, 243, 393, 405, 1035, 1182
Bme1390I CCNGG 2 cut(s) 741, 1092
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 1129
BmgT120I GGNCC 1 cut(s) 19
BmiI GGNNCC 3 cut(s) 341, 626, 935
BmrFI CCNGG 2 cut(s) 741, 1092
BmrI ACTGGG 1 cut(s) 231
BmsI GCATC 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 231
BoxI GACNNNNGTC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 383
BsaBI GATNNNNATC 2 cut(s) 732, 1158
BsaJI CCNNGG 2 cut(s) 54, 802
BsaXI ACNNNNNCTCC 2 cut(s) 1126, 1156
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 90, 615, 647, 705, 803
Bse1I ACTGG 3 cut(s) 226, 535, 768
Bse8I GATNNNNATC 2 cut(s) 732, 1158
BseBI CCWGG 2 cut(s) 741, 1092
BseDI CCNNGG 2 cut(s) 54, 802
BseGI GGATG 3 cut(s) 231, 792, 820
BseJI GATNNNNATC 2 cut(s) 732, 1158
BseLI CCNNNNNNNGG 6 cut(s) 16, 90, 615, 647, 705, 803
BseMII CTCAG 2 cut(s) 378, 1194
BseNI ACTGG 3 cut(s) 226, 535, 768
BseRI GAGGAG 1 cut(s) 630
BseSI GKGCMC 1 cut(s) 444
BseX3I CGGCCG 1 cut(s) 239
BseXI GCAGC 4 cut(s) 61, 415, 1045, 1192
Bsh1236I CGCG 3 cut(s) 614, 645, 783
Bsh1285I CGRYCG 2 cut(s) 242, 736
BshFI GGCC 2 cut(s) 241, 680
BsiEI CGRYCG 2 cut(s) 242, 736
BsiHKAI GWGCWC 2 cut(s) 306, 1287
BsiHKCI CYCGRG 1 cut(s) 1129
BslFI GGGAC 2 cut(s) 232, 821
BslI CCNNNNNNNGG 6 cut(s) 16, 90, 615, 647, 705, 803
BsmFI GGGAC 2 cut(s) 232, 821
BsnI GGCC 2 cut(s) 241, 680
BsoBI CYCGRG 1 cut(s) 1129
Bsp1286I GDGCHC 4 cut(s) 306, 444, 1130, 1287
Bsp1407I TGTACA 1 cut(s) 374
Bsp68I TCGCGA 2 cut(s) 645, 783
BspACI CCGC 3 cut(s) 106, 242, 392
BspANI GGCC 2 cut(s) 241, 680
BspCNI CTCAG 2 cut(s) 379, 1195
BspFNI CGCG 3 cut(s) 614, 645, 783
BspLI GGNNCC 3 cut(s) 341, 626, 935
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 2 cut(s) 30, 945
BspPI GGATC 8 cut(s) 28, 58, 164, 541, 619, 632, 934, 1154
BspTI CTTAAG 1 cut(s) 700
BsrGI TGTACA 1 cut(s) 374
BsrI ACTGG 3 cut(s) 226, 535, 768
BssECI CCNNGG 2 cut(s) 54, 802
BssNAI GTATAC 1 cut(s) 556
BssT1I CCWWGG 1 cut(s) 54
Bst1107I GTATAC 1 cut(s) 556
Bst2UI CCWGG 2 cut(s) 741, 1092
Bst4CI ACNGT 4 cut(s) 117, 695, 1028, 1191
BstAFI CTTAAG 1 cut(s) 700
BstAUI TGTACA 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 635
BstDEI CTNAG 2 cut(s) 387, 1203
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 3 cut(s) 231, 792, 820
BstFNI CGCG 3 cut(s) 614, 645, 783
BstMCI CGRYCG 2 cut(s) 242, 736
BstMWI GCNNNNNNNGC 4 cut(s) 273, 294, 400, 1134
BstNI CCWGG 2 cut(s) 741, 1092
BstNSI RCATGY 2 cut(s) 76, 637
BstPAI GACNNNNGTC 1 cut(s) 735
BstSCI CCNGG 2 cut(s) 739, 1090
BstSFI CTRYAG 1 cut(s) 47
BstSLI GKGCMC 1 cut(s) 444
BstUI CGCG 3 cut(s) 614, 645, 783
BstV1I GCAGC 4 cut(s) 61, 415, 1045, 1192
BstX2I RGATCY 4 cut(s) 33, 533, 624, 926
BstYI RGATCY 4 cut(s) 33, 533, 624, 926
BstZ17I GTATAC 1 cut(s) 556
BstZI CGGCCG 1 cut(s) 239
BsuRI GGCC 2 cut(s) 241, 680
BtgI CCRYGG 1 cut(s) 802
BtgZI GCGATG 1 cut(s) 660
BtsCI GGATG 3 cut(s) 231, 792, 820
BtsIMutI CAGTG 4 cut(s) 528, 681, 700, 775
BtuMI TCGCGA 2 cut(s) 645, 783
BveI ACCTGC 2 cut(s) 30, 945
Cac8I GCNNGC 1 cut(s) 635
Cfr13I GGNCC 1 cut(s) 19
Csp6I GTAC 4 cut(s) 199, 375, 1010, 1253
CviAII CATG 5 cut(s) 73, 175, 634, 745, 1006
CviQI GTAC 4 cut(s) 199, 375, 1010, 1253
DdeI CTNAG 2 cut(s) 387, 1203
EaeI YGGCCR 2 cut(s) 239, 678
EagI CGGCCG 1 cut(s) 239
EclXI CGGCCG 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 1130
Eco47I GGWCC 1 cut(s) 19
Eco52I CGGCCG 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 1129
EcoRI GAATTC 1 cut(s) 94
EcoRII CCWGG 2 cut(s) 739, 1090
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 3 cut(s) 74, 180, 299
EcoT38I GRGCYC 1 cut(s) 1130
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 5 cut(s) 76, 178, 637, 748, 1009
FaqI GGGAC 2 cut(s) 232, 821
FatI CATG 5 cut(s) 72, 174, 633, 744, 1005
FauNDI CATATG 1 cut(s) 1248
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 6 cut(s) 50, 242, 392, 404, 1034, 1181
FokI GGATG 3 cut(s) 238, 779, 827
FriOI GRGCYC 1 cut(s) 1130
Fsp4HI GCNGC 6 cut(s) 50, 242, 392, 404, 1034, 1181
FspBI CTAG 3 cut(s) 576, 1304, 1375
GluI GCNGC 6 cut(s) 50, 242, 392, 404, 1034, 1181
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 2 cut(s) 241, 680
Hin1II CATG 5 cut(s) 76, 178, 637, 748, 1009
HincII GTYRAC 1 cut(s) 964
HindII GTYRAC 1 cut(s) 964
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 5 cut(s) 367, 450, 461, 748, 965
HphI GGTGA 3 cut(s) 115, 1106, 1169
Hpy166II GTNNAC 2 cut(s) 556, 964
Hpy188I TCNGA 7 cut(s) 7, 68, 388, 412, 880, 1204, 1275
Hpy188III TCNNGA 6 cut(s) 361, 644, 758, 782, 1085, 1151
Hpy8I GTNNAC 2 cut(s) 556, 964
HpyAV CCTTC 3 cut(s) 901, 1112, 1226
HpyCH4III ACNGT 4 cut(s) 117, 695, 1028, 1191
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 5 cut(s) 49, 72, 178, 236, 297
HpyF10VI GCNNNNNNNGC 4 cut(s) 273, 294, 400, 1134
HpyF3I CTNAG 2 cut(s) 387, 1203
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 5 cut(s) 76, 178, 637, 748, 1009
LmnI GCTCC 2 cut(s) 1134, 1290
Lsp1109I GCAGC 4 cut(s) 61, 415, 1045, 1192
LweI GCATC 1 cut(s) 59
MaeI CTAG 3 cut(s) 576, 1304, 1375
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 3 cut(s) 919, 1094, 1367
MboII GAAGA 6 cut(s) 90, 442, 721, 1139, 1223, 1241
MflI RGATCY 4 cut(s) 33, 533, 624, 926
MhlI GDGCHC 4 cut(s) 306, 444, 1130, 1287
MluCI AATT 7 cut(s) 94, 231, 547, 865, 888, 1076, 1167
MluI ACGCGT 1 cut(s) 612
MlyI GAGTC 1 cut(s) 959
MmeI TCCRAC 3 cut(s) 159, 633, 686
Mph1103I ATGCAT 3 cut(s) 74, 180, 299
MroXI GAANNNNTTC 1 cut(s) 1236
MseI TTAA 3 cut(s) 30, 159, 701
MslI CAYNNNNRTG 2 cut(s) 309, 726
MspCI CTTAAG 1 cut(s) 700
MspR9I CCNGG 2 cut(s) 741, 1092
MvaI CCWGG 2 cut(s) 741, 1092
MvnI CGCG 3 cut(s) 614, 645, 783
MwoI GCNNNNNNNGC 4 cut(s) 273, 294, 400, 1134
NdeI CATATG 1 cut(s) 1248
NlaIII CATG 5 cut(s) 76, 178, 637, 748, 1009
NlaIV GGNNCC 3 cut(s) 341, 626, 935
NmuCI GTSAC 2 cut(s) 919, 1094
NruI TCGCGA 2 cut(s) 645, 783
NsiI ATGCAT 3 cut(s) 74, 180, 299
NspI RCATGY 2 cut(s) 76, 637
PaeI GCATGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 1236
PfeI GAWTC 4 cut(s) 367, 450, 461, 748
PflMI CCANNNNNTGG 3 cut(s) 90, 615, 803
PkrI GCNGC 6 cut(s) 51, 243, 393, 405, 1035, 1182
Ple19I CGATCG 1 cut(s) 736
PleI GAGTC 1 cut(s) 959
PpsI GAGTC 1 cut(s) 959
PshAI GACNNNNGTC 1 cut(s) 735
Psp6I CCWGG 2 cut(s) 739, 1090
PspGI CCWGG 2 cut(s) 739, 1090
PspN4I GGNNCC 3 cut(s) 341, 626, 935
PspPI GGNCC 1 cut(s) 19
PstI CTGCAG 1 cut(s) 51
PsuI RGATCY 4 cut(s) 33, 533, 624, 926
PvuI CGATCG 1 cut(s) 736
RruI TCGCGA 2 cut(s) 645, 783
RsaI GTAC 4 cut(s) 200, 376, 1011, 1254
RsaNI GTAC 4 cut(s) 199, 375, 1010, 1253
RseI CAYNNNNRTG 2 cut(s) 309, 726
SaqAI TTAA 3 cut(s) 30, 159, 701
SatI GCNGC 6 cut(s) 50, 242, 392, 404, 1034, 1181
Sau96I GGNCC 1 cut(s) 19
SchI GAGTC 1 cut(s) 959
ScrFI CCNGG 2 cut(s) 741, 1092
SduI GDGCHC 4 cut(s) 306, 444, 1130, 1287
SfaNI GCATC 1 cut(s) 59
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 2 cut(s) 309, 726
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
SphI GCATGC 1 cut(s) 637
Sse9I AATT 7 cut(s) 94, 231, 547, 865, 888, 1076, 1167
SsiI CCGC 3 cut(s) 106, 242, 392
SspI AATATT 2 cut(s) 1225, 1326
SspMI CTAG 3 cut(s) 576, 1304, 1375
StyD4I CCNGG 2 cut(s) 739, 1090
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 117, 695, 1028, 1191
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 655, 1196
TasI AATT 7 cut(s) 94, 231, 547, 865, 888, 1076, 1167
TatI WGTACW 1 cut(s) 374
TauI GCSGC 2 cut(s) 244, 394
TfiI GAWTC 4 cut(s) 367, 450, 461, 748
Tru1I TTAA 3 cut(s) 30, 159, 701
Tru9I TTAA 3 cut(s) 30, 159, 701
TscAI CASTG 4 cut(s) 535, 681, 700, 775
TseFI GTSAC 2 cut(s) 919, 1094
TseI GCWGC 4 cut(s) 49, 403, 1033, 1180
Tsp45I GTSAC 2 cut(s) 919, 1094
TspDTI ATGAA 6 cut(s) 109, 242, 431, 584, 597, 920
TspGWI ACGGA 3 cut(s) 791, 937, 949
TspRI CASTG 4 cut(s) 535, 681, 700, 775
Van91I CCANNNNNTGG 3 cut(s) 90, 615, 803
Vha464I CTTAAG 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 2 cut(s) 94, 1076
XceI RCATGY 2 cut(s) 76, 637
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 1 cut(s) 1236
XspI CTAG 3 cut(s) 576, 1304, 1375
Zsp2I ATGCAT 3 cut(s) 74, 180, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.