RLG00000005304

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000424
Physical Location & Seq
Forward (+)
66658549 .. 66661642
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005304

Sequence Viewer

Length: 2520 bp
ATGGAAGGGAAACCTAAATCTGTTTTTATGGTGGCGCATTCTGATGACGGCTCATATAGTGCCCTATATGAAGTGAAAATCCAACCAGGAGGAGAAGTTATAGGCGTCGTAGGCGGAGGCGGAAGATCAGCAGCAACTCATAAAAAACGCCGCCAGCTACTTGAGCCGGTGGTTAAGTTGTTTGATGACAAAGGTATAGTGGTACCGGAGCATTCAAACTTGGCAACTGCAAGGTTCTTCAGCCGCTCCAAATTATACATATGGCTGAACGAAAGCAAGTCTAAAATACTTAACAAAGAGATCACCACTACCAACTCAACATCCACACCCTGCTATGGATATGTTTTGGACACGGAGACGAAGTCATTGGTCCCGTTCAAGTCATCTCAAGCACATAAGCCATCAGCAACTGTTATTTCCGCCTTTGGTAAGCTTTATCTTCTTGCGAATCCAGAATGCTGGCCCGAGATCCCAACCCCGGCCTTTGAGCGGTATGATCCTGCCACTAATTGTTGGGAATCCCTCCCTCCTTTTCCAGGCTATAATCACCGGAAAGAGATTAGAATAATAGGTTATGCCGTCTGTTCCAGCTTTATTCTGTTCACAGCAGACCACCATAAGGAATACGTGGCATTCAATGTGACCAGAAAACAATGGGCTTTTGATGTGACCAGAAAACATTGGCATGAAGTCAAAGTGTGGCCGCCTACTCAATATCGTTTAACGAATGGTTCAGTCGGCCGGGGGAGGGCCGTGGTTGTAGGTGATACTGTCTATACCTTTAACTATTCATGTACTAGTGTTGTGGCATTCAACTTTTGGTGGGATTTAGATGAGGATGGTGATGTTACCTTCTATGTAAGCCCAGAATCCATTTTGAAAGGCTTGGAGATTAGGTTGGGGACAAAAGGAAAGAAAGGAGGGTATTTGGTTCATTTGGGGAACTTGGATTTTTGTCTTGTCCAAAATTTCTTCCAACTTAGTAGAGCTGGTCGTCAAGAATTTTGTATCACGACGTTTCAGGTTGTGGTCGACGGAGGAGGAAGGCATATCAAGACCTTACATTCAACTATTTGTGAACTGGACACCCCCGACTCTAATTTCATTTGTTTAAGTTACTGCTTGACTCTAGATGTTGGTGATATTGAACCCAAAGAAGAAGAGAGAGTGATCACTACCAGGTCCATGCAACAGAAAGAACAAGGTGTTGTTTCAACAAATGAATTAGAAGAAACAAAGCAATGCAGCCTTTTCGGCACATCAGACATCCCACAAGAGTTGATCTTTGACATCCTTGCAAGGCTGTCAACCAAAGATTTGATTAGACTCGTGTGCGTTTCTAAAGCATGGAATGCTGCCATCCAAGACCCACAATTGGCCAAACTGCATCTCTGTCTCTCCATCAAGATCATCAACTCTTTGGATCCCACCTTTTATATTCTTTCATCTGTACATCTATCAGATGACTTTTATCCAGTGGCCTTGTTCAACAATAACACAAAGGGTAGAGTTGTCAAAGTTAAACATCGATCAAAACAGGTTCAGCAAAGAGCTAGCATCTTAGGTTACTGTAATGGTTTGCTTTGCATTTCTGGATGGTCTAGGGATGCCAATGAATATGAAGACTTTGGTTTGTGGAATCCAACAATACAAAAGTTCAAGAAGGTTCCATTGTCAGCCTTCAGTAAGTTCACGAAGAGGGAAACCTTTTATGGATTCGGGTATGATTCAGTTAACGACGATTACAAATTTGTGAGATTGGCACAGTTTGAGGATCATCATCGGAGTGGTGTTGTTACAAGTTCGGAAGTGCAGGTCTATAGTCTAAAATCGCACTCATGGAAAAGGATCCAAGACTTGCCTGCTCATCTTAACAAAGATTTTGAACTTGCCTCTATAGGGGTTTGTGTTGACGGTGCTTTACATTGGTTGATGAGATTACGTGGTGAAAGTGTAAGAGGGATGATAGTTCTAACCCTTCATCTCACAACTGAAGAGTATCACTGGTTTTCTACCCCTGACTATAATTATCCATGTCGCTGTGCAAATTCAAAAGACGAGCGTAAAAACAGGCACTGTTTGTCTTTGCAAGTTTTGGGAGGGTGCTTATGTTTTCGATTCCAATGTGATCTCCTGGATGCTTGGATTTTGAAGGAAAATGGAGTTGCCGAATCATGGACTAAACTTTATTCTACCAAGGAATTGCCCGAGTTATTTTCCTGCCAGCCTTTGATGCATTCTGGGTGTGGTAAAATGGTTCTTTTCTCAAATCACTTTGGGAACACTTTCTGGTTTGATTTAGAGAATAAGAAACGTAAAGTGGTGGCCAGACCCGATGAACGTGCTTTTGGCTTTGACACGTTAATTACTCGTCAAACCCTTCATCTCCTTAGGAGGCGACCAGAGCTTCCTTCAAAAGATGGGCATAACAGCCGAATTAGTCGCAACCCCAAAAGGTTTTTACAAGCCGGAAACTTCGGTTTCTTGTGCTTTGAAGCAGCCAACTGGCAGCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

840

Amino Acids

95.6

Weight (kDa)

8.68

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 113 - 234 4.6e-08 Protein of unknown function (DUF1668)
F-box PF00646 421 - 459 4.4e-09 F-box domain
F-box-like PF12937 421 - 459 6.7e-09 F-box-like
FBA_3 PF08268 505 - 676 8.5e-18 F-box associated beta propeller domain
FBA_1 PF07734 508 - 766 2.4e-22 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32600 AT1G54640
fragaria_vesca FvH4_2g26290 FvH4_2g26290 FvH4_2g26300 FvH4_5g16160 FvH4_6g49561 FvH4_6g49581
malus_domestica MD02G1261500.v1.1 MD02G1290400.v1.1 MD02G1290700.v1.1 MD02G1290800.v1.1 MD05G1183700.v1.1 MD07G1058700.v1.1 MD07G1058900.v1.1 MD07G1059200.v1.1 MD08G1079300.v1.1 MD08G1079600.v1.1 MD16G1218300.v1.1 MD16G1218400.v1.1 MD16G1218600.v1.1 MD16G1218700.v1.1 MD17G1102900.v1.1
prunus_persica Prupe.1G024400_v2.0.a1 Prupe.1G024500_v2.0.a1 Prupe.1G024600_v2.0.a1 Prupe.1G024800_v2.0.a1 Prupe.1G025300_v2.0.a1 Prupe.1G025400_v2.0.a1 Prupe.1G556300_v2.0.a1 Prupe.3G094100_v2.0.a1 Prupe.3G094200_v2.0.a1
pyrus_communis pycom02g22350 pycom05g27240 pycom07g04530 pycom07g04540 pycom08g06570 pycom10g07370 pycom16g17130 pycom16g17140 pycom16g17150 pycom16g17160 pycom16g17180 pycom17g09720
rosa_chinensis RchiOBHm_Chr6g0294901 RchiOBHm_Chr7g0179991 RchiOBHm_Chr7g0180101 RchiOBHm_Chr7g0180241
rosa_laevigata RLG00000005281 RLG00000005282 RLG00000005293 RLG00000005304 RLG00000011829 RLG00000011831 RLG00000011832 RLG00000021887
rosa_multiflora Rmu_co8519977.1_g000001 Rmu_sc0008966.1_g000002 Rmu_sc0008966.1_g000003 Rmu_sc0008966.1_g000008 Rmu_sc0011963.1_g000010 Rmu_ssc0000119.1_g000012 Rmu_ssc0000119.1_g000032
rosa_roxburghii Rroxscaffold_2G00081970 Rroxscaffold_2G00081980 Rroxscaffold_3G00273030 Rroxscaffold_3G00273250 Rroxscaffold_7G00172880 Rroxscaffold_7G00172900
rosa_rugosa Rorug02G0541400 Rorug06G0429600
rosa_samantha Rh2AG613800 Rh2CG595200 Rh2CG595300 Rh6AG361300 Rh6BG368000 Rh6BG368100 Rh6BG368300 Rh6BG368500 Rh6BG368600 Rh6CG375200 Rh6CG375300 Rh7AG030100 Rh7AG031000 Rh7AG032000 Rh7BG029400 Rh7BG029500 Rh7BG032100 Rh7CG031100 Rh7CG032700 Rh7CG032800 Rh7CG033900 Rh7DG030400 Rh7DG032400
rosa_wichuraiana Rw7G002420 Rw7G002670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1804
Acc65I GGTACC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 202
AccBSI CCGCTC 2 cut(s) 246, 490
AccI GTMKAC 1 cut(s) 1032
AciI CCGC 7 cut(s) 114, 120, 151, 244, 420, 490, 704
AclWI GGATC 7 cut(s) 463, 491, 1418, 1431, 1782, 1843, 1856
AcoI YGGCCR 4 cut(s) 701, 739, 1377, 2325
AcsI RAATTY 4 cut(s) 967, 1001, 1748, 2047
AcuI CTGAAG 3 cut(s) 223, 1666, 2013
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 3 cut(s) 204, 796, 1452
AfiI CCNNNNNNNGG 9 cut(s) 335, 478, 489, 536, 619, 748, 1375, 1899, 2175
AflIII ACRYGT 1 cut(s) 2358
AhlI ACTAGT 1 cut(s) 797
AjnI CCWGG 4 cut(s) 85, 535, 1178, 2133
AjuI GAANNNNNNNTTGG 2 cut(s) 2331, 2363
AluBI AGCT 6 cut(s) 157, 433, 591, 989, 1553, 2407
AluI AGCT 6 cut(s) 157, 433, 591, 989, 1553, 2407
Alw26I GTCTC 2 cut(s) 350, 1400
AlwI GGATC 7 cut(s) 463, 491, 1418, 1431, 1782, 1843, 1856
AlwNI CAGNNNCTG 2 cut(s) 410, 2076
Ama87I CYCGRG 2 cut(s) 464, 2207
AoxI GGCC 8 cut(s) 461, 480, 701, 739, 750, 1377, 1479, 2325
ApeKI GCWGC 5 cut(s) 131, 1245, 1355, 2498, 2509
ApoI RAATTY 4 cut(s) 967, 1001, 1748, 2047
Asp700I GAANNNNTTC 1 cut(s) 2285
Asp718I GGTACC 1 cut(s) 202
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 4 cut(s) 370, 462, 750, 1182
AsuC2I CCSGG 2 cut(s) 479, 743
AsuHPI GGTGA 6 cut(s) 295, 539, 776, 854, 1151, 1958
AsuNHI GCTAGC 1 cut(s) 1553
AvaI CYCGRG 2 cut(s) 464, 2207
AvaII GGWCC 2 cut(s) 370, 1182
AxyI CCTNAGG 1 cut(s) 2390
BaeGI GKGCMC 1 cut(s) 64
BalI TGGCCA 2 cut(s) 1379, 2327
BamHI GGATCC 2 cut(s) 1423, 1848
BanI GGYRCC 1 cut(s) 202
BauI CACGAG 1 cut(s) 1328
BbsI GAAGAC 1 cut(s) 1629
BbvI GCAGC 4 cut(s) 143, 1257, 1342, 2510
BccI CCATC 6 cut(s) 409, 833, 1367, 1409, 1590, 2414
BceAI ACGGC 3 cut(s) 64, 563, 737
BcgI CGANNNNNNTGC 4 cut(s) 1234, 1268, 2324, 2358
BciT130I CCWGG 4 cut(s) 87, 537, 1180, 2135
BclI TGATCA 1 cut(s) 1170
BcnI CCSGG 2 cut(s) 479, 743
BcoDI GTCTC 2 cut(s) 350, 1400
BcuI ACTAGT 1 cut(s) 797
BfaI CTAG 4 cut(s) 798, 1130, 1554, 1602
BfmI CTRYAG 2 cut(s) 1819, 1896
BfuAI ACCTGC 1 cut(s) 1804
BglI GCCNNNNNGGC 1 cut(s) 1254
BisI GCNGC 8 cut(s) 132, 151, 244, 704, 1246, 1356, 2499, 2510
BlsI GCNGC 8 cut(s) 133, 152, 245, 705, 1247, 1357, 2500, 2511
Bme1390I CCNGG 6 cut(s) 87, 479, 537, 743, 1180, 2135
Bme18I GGWCC 2 cut(s) 370, 1182
BmeT110I CYCGRG 2 cut(s) 464, 2207
BmgT120I GGNCC 4 cut(s) 370, 462, 750, 1182
BmiI GGNNCC 5 cut(s) 204, 372, 1425, 1668, 1850
BmrFI CCNGG 6 cut(s) 87, 479, 537, 743, 1180, 2135
BmsI GCATC 5 cut(s) 1396, 1566, 1597, 2128, 2223
BmtI GCTAGC 1 cut(s) 1557
BpiI GAAGAC 1 cut(s) 1629
BpuEI CTTGAG 2 cut(s) 182, 372
BpuMI CCSGG 2 cut(s) 479, 743
Bsa29I ATCGAT 1 cut(s) 1528
BsaAI YACGTR 2 cut(s) 628, 1943
BsaBI GATNNNNATC 1 cut(s) 1779
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 4 cut(s) 477, 742, 753, 2196
BsaWI WCCGGW 2 cut(s) 205, 549
BsaXI ACNNNNNCTCC 6 cut(s) 81, 111, 347, 377, 881, 911
Bsc4I CCNNNNNNNGG 9 cut(s) 335, 478, 489, 536, 619, 748, 1375, 1899, 2175
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 4 cut(s) 1086, 1475, 2009, 2510
Bse21I CCTNAGG 1 cut(s) 2390
Bse3DI GCAATG 1 cut(s) 1247
Bse8I GATNNNNATC 1 cut(s) 1779
BseBI CCWGG 4 cut(s) 87, 537, 1180, 2135
BseCI ATCGAT 1 cut(s) 1528
BseDI CCNNGG 4 cut(s) 477, 742, 753, 2196
BseGI GGATG 9 cut(s) 320, 844, 1266, 1290, 1359, 1601, 1612, 1968, 2143
BseJI GATNNNNATC 1 cut(s) 1779
BseLI CCNNNNNNNGG 9 cut(s) 335, 478, 489, 536, 619, 748, 1375, 1899, 2175
BseMI GCAATG 1 cut(s) 1247
BseNI ACTGG 4 cut(s) 1086, 1475, 2009, 2510
BseRI GAGGAG 2 cut(s) 105, 1053
BseSI GKGCMC 1 cut(s) 64
BseX3I CGGCCG 1 cut(s) 739
BseXI GCAGC 4 cut(s) 143, 1257, 1342, 2510
BsgI GTGCAG 1 cut(s) 1832
Bsh1285I CGRYCG 1 cut(s) 742
BshFI GGCC 8 cut(s) 463, 482, 703, 741, 752, 1379, 1481, 2327
BshNI GGYRCC 1 cut(s) 202
BshVI ATCGAT 1 cut(s) 1528
BsiEI CGRYCG 1 cut(s) 742
BsiHKCI CYCGRG 2 cut(s) 464, 2207
BsiSI CCGG 6 cut(s) 167, 206, 479, 550, 742, 2469
BslFI GGGAC 2 cut(s) 356, 916
BslI CCNNNNNNNGG 9 cut(s) 335, 478, 489, 536, 619, 748, 1375, 1899, 2175
BsmAI GTCTC 2 cut(s) 350, 1400
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 2 cut(s) 356, 916
BsmI GAATGC 7 cut(s) 37, 211, 461, 632, 809, 1357, 2236
BsnI GGCC 8 cut(s) 463, 482, 703, 741, 752, 1379, 1481, 2327
BsoBI CYCGRG 2 cut(s) 464, 2207
Bsp1286I GDGCHC 1 cut(s) 64
Bsp1407I TGTACA 1 cut(s) 1450
BspACI CCGC 7 cut(s) 114, 120, 151, 244, 420, 490, 704
BspANI GGCC 8 cut(s) 463, 482, 703, 741, 752, 1379, 1481, 2327
BspDI ATCGAT 1 cut(s) 1528
BspLI GGNNCC 5 cut(s) 204, 372, 1425, 1668, 1850
BspMI ACCTGC 1 cut(s) 1804
BspOI GCTAGC 1 cut(s) 1557
BspPI GGATC 7 cut(s) 463, 491, 1418, 1431, 1782, 1843, 1856
BspT107I GGYRCC 1 cut(s) 202
BsrBI CCGCTC 2 cut(s) 246, 490
BsrDI GCAATG 1 cut(s) 1247
BsrFI RCCGGY 1 cut(s) 166
BsrGI TGTACA 1 cut(s) 1450
BsrI ACTGG 4 cut(s) 1086, 1475, 2009, 2510
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 4 cut(s) 477, 742, 753, 2196
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 1328
BssT1I CCWWGG 1 cut(s) 2196
Bst2BI CACGAG 1 cut(s) 1328
Bst2UI CCWGG 4 cut(s) 87, 537, 1180, 2135
Bst4CI ACNGT 6 cut(s) 412, 772, 1571, 1767, 1916, 2078
Bst6I CTCTTC 3 cut(s) 1155, 1691, 1989
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 1352
BstAUI TGTACA 1 cut(s) 1450
BstBAI YACGTR 2 cut(s) 628, 1943
BstC8I GCNNGC 5 cut(s) 155, 461, 1555, 1863, 2225
BstDEI CTNAG 3 cut(s) 980, 1561, 2390
BstDSI CCRYGG 1 cut(s) 753
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 9 cut(s) 320, 844, 1266, 1290, 1359, 1601, 1612, 1968, 2143
BstHHI GCGC 1 cut(s) 37
BstMAI GTCTC 2 cut(s) 350, 1400
BstMCI CGRYCG 1 cut(s) 742
BstMWI GCNNNNNNNGC 6 cut(s) 111, 163, 1254, 1352, 2233, 2404
BstNI CCWGG 4 cut(s) 87, 537, 1180, 2135
BstSCI CCNGG 6 cut(s) 85, 477, 535, 741, 1178, 2133
BstSFI CTRYAG 2 cut(s) 1819, 1896
BstSLI GKGCMC 1 cut(s) 64
BstV1I GCAGC 4 cut(s) 143, 1257, 1342, 2510
BstV2I GAAGAC 1 cut(s) 1629
BstX2I RGATCY 3 cut(s) 468, 1423, 1848
BstXI CCANNNNNNTGG 1 cut(s) 459
BstYI RGATCY 3 cut(s) 468, 1423, 1848
BstZI CGGCCG 1 cut(s) 739
Bsu15I ATCGAT 1 cut(s) 1528
Bsu36I CCTNAGG 1 cut(s) 2390
BsuRI GGCC 8 cut(s) 463, 482, 703, 741, 752, 1379, 1481, 2327
BsuTUI ATCGAT 1 cut(s) 1528
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 9 cut(s) 320, 844, 1266, 1290, 1359, 1601, 1612, 1968, 2143
BtsIMutI CAGTG 3 cut(s) 1482, 2002, 2074
BveI ACCTGC 1 cut(s) 1804
Cac8I GCNNGC 5 cut(s) 155, 461, 1555, 1863, 2225
CaiI CAGNNNCTG 2 cut(s) 410, 2076
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 4 cut(s) 370, 462, 750, 1182
ClaI ATCGAT 1 cut(s) 1528
CseI GACGC 1 cut(s) 94
CsiI ACCWGGT 1 cut(s) 1178
Csp6I GTAC 3 cut(s) 203, 795, 1451
CspCI CAANNNNNGTGG 2 cut(s) 1260, 1295
CviAII CATG 7 cut(s) 686, 792, 1186, 1347, 1839, 2034, 2175
CviQI GTAC 3 cut(s) 203, 795, 1451
DdeI CTNAG 3 cut(s) 980, 1561, 2390
EaeI YGGCCR 4 cut(s) 701, 739, 1377, 2325
EagI CGGCCG 1 cut(s) 739
Eam1104I CTCTTC 3 cut(s) 1155, 1691, 1989
EarI CTCTTC 3 cut(s) 1155, 1691, 1989
EciI GGCGGA 3 cut(s) 129, 135, 409
EclXI CGGCCG 1 cut(s) 739
Eco130I CCWWGG 1 cut(s) 2196
Eco47I GGWCC 2 cut(s) 370, 1182
Eco52I CGGCCG 1 cut(s) 739
Eco57I CTGAAG 3 cut(s) 223, 1666, 2013
Eco81I CCTNAGG 1 cut(s) 2390
Eco88I CYCGRG 2 cut(s) 464, 2207
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRII CCWGG 4 cut(s) 85, 535, 1178, 2133
EcoT14I CCWWGG 1 cut(s) 2196
EcoT22I ATGCAT 1 cut(s) 2238
ErhI CCWWGG 1 cut(s) 2196
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 7 cut(s) 689, 795, 1189, 1350, 1842, 2037, 2178
FaqI GGGAC 2 cut(s) 356, 916
FatI CATG 7 cut(s) 685, 791, 1185, 1346, 1838, 2033, 2174
FauNDI CATATG 1 cut(s) 260
FbaI TGATCA 1 cut(s) 1170
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 8 cut(s) 132, 151, 244, 704, 1246, 1356, 2499, 2510
FokI GGATG 9 cut(s) 307, 851, 1253, 1277, 1346, 1608, 1619, 1975, 2150
Fsp4HI GCNGC 8 cut(s) 132, 151, 244, 704, 1246, 1356, 2499, 2510
FspBI CTAG 4 cut(s) 798, 1130, 1554, 1602
GlaI GCGC 1 cut(s) 36
GluI GCNGC 8 cut(s) 132, 151, 244, 704, 1246, 1356, 2499, 2510
HaeIII GGCC 8 cut(s) 463, 482, 703, 741, 752, 1379, 1481, 2327
HapII CCGG 6 cut(s) 167, 206, 479, 550, 742, 2469
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 37
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 7 cut(s) 689, 795, 1189, 1350, 1842, 2037, 2178
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 4 cut(s) 1033, 1308, 1735, 1912
HindII GTYRAC 4 cut(s) 1033, 1308, 1735, 1912
HindIII AAGCTT 1 cut(s) 431
HpaI GTTAAC 1 cut(s) 1735
HpaII CCGG 6 cut(s) 167, 206, 479, 550, 742, 2469
HphI GGTGA 6 cut(s) 295, 539, 776, 854, 1151, 1958
Hpy166II GTNNAC 7 cut(s) 603, 1033, 1079, 1308, 1692, 1735, 1912
Hpy188I TCNGA 5 cut(s) 43, 1264, 1462, 1785, 1807
Hpy188III TCNNGA 9 cut(s) 452, 998, 1012, 1054, 1130, 1405, 1593, 1660, 1693
Hpy8I GTNNAC 7 cut(s) 603, 1033, 1079, 1308, 1692, 1735, 1912
Hpy99I CGWCG 4 cut(s) 110, 1018, 1037, 1742
HpyAV CCTTC 8 cut(s) 862, 1038, 1657, 1690, 1988, 2146, 2390, 2421
HpyCH4III ACNGT 6 cut(s) 412, 772, 1571, 1767, 1916, 2078
HpyCH4IV ACGT 6 cut(s) 627, 1016, 1942, 2314, 2341, 2360
HpyF10VI GCNNNNNNNGC 6 cut(s) 111, 163, 1254, 1352, 2233, 2404
HpyF3I CTNAG 3 cut(s) 980, 1561, 2390
HpySE526I ACGT 6 cut(s) 627, 1016, 1942, 2314, 2341, 2360
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 7 cut(s) 689, 795, 1189, 1350, 1842, 2037, 2178
HspAI GCGC 1 cut(s) 35
KpnI GGTACC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 1170
KspAI GTTAAC 1 cut(s) 1735
LmnI GCTCC 2 cut(s) 208, 251
Lsp1109I GCAGC 4 cut(s) 143, 1257, 1342, 2510
LweI GCATC 5 cut(s) 1396, 1566, 1597, 2128, 2223
MabI ACCWGGT 1 cut(s) 1178
MaeI CTAG 4 cut(s) 798, 1130, 1554, 1602
MaeII ACGT 6 cut(s) 627, 1016, 1942, 2314, 2341, 2360
MaeIII GTNAC 6 cut(s) 640, 667, 847, 1115, 1565, 1795
MbiI CCGCTC 2 cut(s) 246, 490
MfeI CAATTG 1 cut(s) 1373
MflI RGATCY 3 cut(s) 468, 1423, 1848
MhlI GDGCHC 1 cut(s) 64
MlsI TGGCCA 2 cut(s) 1379, 2327
MluNI TGGCCA 2 cut(s) 1379, 2327
MlyI GAGTC 3 cut(s) 1088, 1120, 1320
MmeI TCCRAC 3 cut(s) 106, 1000, 1667
Mox20I TGGCCA 2 cut(s) 1379, 2327
Mph1103I ATGCAT 1 cut(s) 2238
MroXI GAANNNNTTC 1 cut(s) 2285
MscI TGGCCA 2 cut(s) 1379, 2327
MseI TTAA 9 cut(s) 174, 291, 722, 783, 1112, 1521, 1734, 1872, 2363
MslI CAYNNNNRTG 3 cut(s) 42, 684, 1785
Msp20I TGGCCA 2 cut(s) 1379, 2327
MspI CCGG 6 cut(s) 167, 206, 479, 550, 742, 2469
MspR9I CCNGG 6 cut(s) 87, 479, 537, 743, 1180, 2135
MunI CAATTG 1 cut(s) 1373
Mva1269I GAATGC 7 cut(s) 37, 211, 461, 632, 809, 1357, 2236
MvaI CCWGG 4 cut(s) 87, 537, 1180, 2135
MwoI GCNNNNNNNGC 6 cut(s) 111, 163, 1254, 1352, 2233, 2404
NciI CCSGG 2 cut(s) 479, 743
NdeI CATATG 1 cut(s) 260
NheI GCTAGC 1 cut(s) 1553
NlaIII CATG 7 cut(s) 689, 795, 1189, 1350, 1842, 2037, 2178
NlaIV GGNNCC 5 cut(s) 204, 372, 1425, 1668, 1850
NmuCI GTSAC 2 cut(s) 640, 667
NsiI ATGCAT 1 cut(s) 2238
PctI GAATGC 7 cut(s) 37, 211, 461, 632, 809, 1357, 2236
PdmI GAANNNNTTC 1 cut(s) 2285
PfeI GAWTC 8 cut(s) 448, 518, 869, 1639, 1716, 1727, 2118, 2171
PflFI GACNNNGTC 1 cut(s) 361
PfoI TCCNGGA 1 cut(s) 2133
PkrI GCNGC 8 cut(s) 133, 152, 245, 705, 1247, 1357, 2500, 2511
PleI GAGTC 3 cut(s) 1088, 1120, 1320
PpsI GAGTC 3 cut(s) 1088, 1120, 1320
Ppu21I YACGTR 2 cut(s) 628, 1943
Psp6I CCWGG 4 cut(s) 85, 535, 1178, 2133
PspGI CCWGG 4 cut(s) 85, 535, 1178, 2133
PspN4I GGNNCC 5 cut(s) 204, 372, 1425, 1668, 1850
PspPI GGNCC 4 cut(s) 370, 462, 750, 1182
PstNI CAGNNNCTG 2 cut(s) 410, 2076
PsuI RGATCY 3 cut(s) 468, 1423, 1848
PsyI GACNNNGTC 1 cut(s) 361
RsaI GTAC 3 cut(s) 204, 796, 1452
RsaNI GTAC 3 cut(s) 203, 795, 1451
RseI CAYNNNNRTG 3 cut(s) 42, 684, 1785
SalI GTCGAC 1 cut(s) 1031
SaqAI TTAA 9 cut(s) 174, 291, 722, 783, 1112, 1521, 1734, 1872, 2363
SatI GCNGC 8 cut(s) 132, 151, 244, 704, 1246, 1356, 2499, 2510
Sau96I GGNCC 4 cut(s) 370, 462, 750, 1182
SchI GAGTC 3 cut(s) 1088, 1120, 1320
ScrFI CCNGG 6 cut(s) 87, 479, 537, 743, 1180, 2135
SduI GDGCHC 1 cut(s) 64
SexAI ACCWGGT 1 cut(s) 1178
SfaNI GCATC 5 cut(s) 1396, 1566, 1597, 2128, 2223
SfcI CTRYAG 2 cut(s) 1819, 1896
SinI GGWCC 2 cut(s) 370, 1182
SmiMI CAYNNNNRTG 3 cut(s) 42, 684, 1785
SmlI CTYRAG 2 cut(s) 161, 387
SmoI CTYRAG 2 cut(s) 161, 387
SpeI ACTAGT 1 cut(s) 797
SsiI CCGC 7 cut(s) 114, 120, 151, 244, 420, 490, 704
SspMI CTAG 4 cut(s) 798, 1130, 1554, 1602
StyD4I CCNGG 6 cut(s) 85, 477, 535, 741, 1178, 2133
StyI CCWWGG 1 cut(s) 2196
TaaI ACNGT 6 cut(s) 412, 772, 1571, 1767, 1916, 2078
TaiI ACGT 6 cut(s) 630, 1019, 1945, 2317, 2344, 2363
TaqI TCGA 3 cut(s) 1032, 1528, 2116
TatI WGTACW 2 cut(s) 794, 1450
TauI GCSGC 3 cut(s) 153, 246, 706
TfiI GAWTC 8 cut(s) 448, 518, 869, 1639, 1716, 1727, 2118, 2171
Tru1I TTAA 9 cut(s) 174, 291, 722, 783, 1112, 1521, 1734, 1872, 2363
Tru9I TTAA 9 cut(s) 174, 291, 722, 783, 1112, 1521, 1734, 1872, 2363
TscAI CASTG 3 cut(s) 1482, 2009, 2081
TseFI GTSAC 2 cut(s) 640, 667
TseI GCWGC 5 cut(s) 131, 1245, 1355, 2498, 2509
Tsp45I GTSAC 2 cut(s) 640, 667
TspGWI ACGGA 2 cut(s) 368, 1050
TspRI CASTG 3 cut(s) 1482, 2009, 2081
Tth111I GACNNNGTC 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 370, 1182
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 4 cut(s) 967, 1001, 1748, 2047
XbaI TCTAGA 1 cut(s) 1129
XmiI GTMKAC 1 cut(s) 1032
XmnI GAANNNNTTC 1 cut(s) 2285
XspI CTAG 4 cut(s) 798, 1130, 1554, 1602
Zsp2I ATGCAT 1 cut(s) 2238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.