pycom2246g00070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002246
OG0000104
Physical Location & Seq
Forward (+)
6641 .. 6874
234 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2246g00070.1

Sequence Viewer

Length: 234 bp
ATGGGCACGGGGGTCCCAGCCTGGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGAAAGACTGGGAACGGTCCCTCGGGGGCCTTCCCCGGGCAGCGAACATTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCTGCGGATGCTAACGCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

78

Amino Acids

8.08

Weight (kDa)

6.7

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 73
AccII CGCG 2 cut(s) 64, 83
AciI CCGC 6 cut(s) 42, 62, 64, 73, 81, 215
AcoI YGGCCR 1 cut(s) 89
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 3 cut(s) 87, 94, 131
AjnI CCWGG 1 cut(s) 20
AluBI AGCT 2 cut(s) 55, 149
AluI AGCT 2 cut(s) 55, 149
Ama87I CYCGRG 3 cut(s) 50, 117, 130
AoxI GGCC 2 cut(s) 89, 122
ApeKI GCWGC 2 cut(s) 55, 135
AspS9I GGNCC 4 cut(s) 13, 112, 122, 209
AsuC2I CCSGG 3 cut(s) 93, 131, 132
AvaI CYCGRG 3 cut(s) 50, 117, 130
AvaII GGWCC 3 cut(s) 13, 112, 209
BaeGI GKGCMC 1 cut(s) 8
BbvI GCAGC 2 cut(s) 42, 147
BccI CCATC 1 cut(s) 200
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 1 cut(s) 22
BcnI CCSGG 3 cut(s) 93, 131, 132
BisI GCNGC 4 cut(s) 56, 65, 81, 136
BlsI GCNGC 4 cut(s) 57, 66, 82, 137
Bme1390I CCNGG 4 cut(s) 22, 93, 131, 132
Bme18I GGWCC 3 cut(s) 13, 112, 209
BmeT110I CYCGRG 3 cut(s) 50, 117, 130
BmgT120I GGNCC 4 cut(s) 13, 112, 122, 209
BmiI GGNNCC 5 cut(s) 14, 15, 26, 114, 123
BmrFI CCNGG 4 cut(s) 22, 93, 131, 132
BmrI ACTGGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 208
BmuI ACTGGG 1 cut(s) 113
BpuMI CCSGG 3 cut(s) 93, 131, 132
BsaJI CCNNGG 5 cut(s) 62, 92, 116, 129, 130
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 94, 131
Bse118I RCCGGY 1 cut(s) 87
Bse1I ACTGG 2 cut(s) 108, 161
Bse3DI GCAATG 2 cut(s) 199, 234
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 5 cut(s) 62, 92, 116, 129, 130
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 3 cut(s) 87, 94, 131
BseMI GCAATG 2 cut(s) 199, 234
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 2 cut(s) 108, 161
BseSI GKGCMC 1 cut(s) 8
BseX3I CGGCCG 1 cut(s) 89
BseXI GCAGC 2 cut(s) 42, 147
BseYI CCCAGC 1 cut(s) 16
Bsh1236I CGCG 2 cut(s) 64, 83
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 2 cut(s) 91, 124
BsiEI CGRYCG 1 cut(s) 92
BsiHKCI CYCGRG 3 cut(s) 50, 117, 130
BsiSI CCGG 3 cut(s) 88, 92, 131
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 3 cut(s) 87, 94, 131
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 2 cut(s) 91, 124
BsoBI CYCGRG 3 cut(s) 50, 117, 130
Bsp1286I GDGCHC 1 cut(s) 8
BspACI CCGC 6 cut(s) 42, 62, 64, 73, 81, 215
BspANI GGCC 2 cut(s) 91, 124
BspCNI CTCAG 1 cut(s) 163
BspFNI CGCG 2 cut(s) 64, 83
BspLI GGNNCC 5 cut(s) 14, 15, 26, 114, 123
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 2 cut(s) 199, 234
BsrFI RCCGGY 1 cut(s) 87
BsrI ACTGG 2 cut(s) 108, 161
BssAI RCCGGY 1 cut(s) 87
BssECI CCNNGG 5 cut(s) 62, 92, 116, 129, 130
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 2 cut(s) 112, 182
BstC8I GCNNGC 2 cut(s) 85, 89
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 2 cut(s) 64, 83
BstMCI CGRYCG 1 cut(s) 92
BstMWI GCNNNNNNNGC 4 cut(s) 55, 61, 64, 70
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 4 cut(s) 20, 91, 129, 130
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 2 cut(s) 64, 83
BstV1I GCAGC 2 cut(s) 42, 147
BstZI CGGCCG 1 cut(s) 89
BsuRI GGCC 2 cut(s) 91, 124
BtgI CCRYGG 1 cut(s) 62
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 85, 89
Cfr10I RCCGGY 1 cut(s) 87
Cfr13I GGNCC 4 cut(s) 13, 112, 122, 209
Cfr42I CCGCGG 1 cut(s) 65
Cfr9I CCCGGG 1 cut(s) 130
Csp6I GTAC 1 cut(s) 160
CviJI RGCY 6 cut(s) 20, 35, 55, 91, 124, 149
CviKI_1 RGCY 6 cut(s) 20, 35, 55, 91, 124, 149
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 89
EagI CGGCCG 1 cut(s) 89
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 89
Eco47I GGWCC 3 cut(s) 13, 112, 209
Eco52I CGGCCG 1 cut(s) 89
Eco88I CYCGRG 3 cut(s) 50, 117, 130
EcoO109I RGGNCCY 2 cut(s) 13, 122
EcoRII CCWGG 1 cut(s) 20
FaqI GGGAC 1 cut(s) 98
FauI CCCGC 2 cut(s) 66, 69
Fnu4HI GCNGC 4 cut(s) 56, 65, 81, 136
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 4 cut(s) 56, 65, 81, 136
GluI GCNGC 4 cut(s) 56, 65, 81, 136
GsaI CCCAGC 1 cut(s) 20
HaeIII GGCC 2 cut(s) 91, 124
HapII CCGG 3 cut(s) 88, 92, 131
HinfI GANTC 1 cut(s) 173
HpaII CCGG 3 cut(s) 88, 92, 131
Hpy188I TCNGA 2 cut(s) 153, 178
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 135
HpyCH4III ACNGT 2 cut(s) 112, 182
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 61, 64, 70
HpyF3I CTNAG 1 cut(s) 150
KflI GGGWCCC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 87
KroNI GCCGGC 1 cut(s) 89
KspI CCGCGG 1 cut(s) 65
LmnI GCTCC 1 cut(s) 63
LpnPI CCDG 9 cut(s) 7, 30, 34, 89, 101, 105, 142, 144, 225
Lsp1109I GCAGC 2 cut(s) 42, 147
LweI GCATC 1 cut(s) 208
MbiI CCGCTC 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 1 cut(s) 126
MroNI GCCGGC 1 cut(s) 87
MseI TTAA 2 cut(s) 185, 189
MslI CAYNNNNRTG 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 3 cut(s) 88, 92, 131
MspR9I CCNGG 4 cut(s) 22, 93, 131, 132
MvaI CCWGG 1 cut(s) 22
MvnI CGCG 2 cut(s) 64, 83
MwoI GCNNNNNNNGC 4 cut(s) 55, 61, 64, 70
NaeI GCCGGC 1 cut(s) 89
NciI CCSGG 3 cut(s) 93, 131, 132
NgoMIV GCCGGC 1 cut(s) 87
NlaIV GGNNCC 5 cut(s) 14, 15, 26, 114, 123
PacI TTAATTAA 1 cut(s) 189
PaeR7I CTCGAG 1 cut(s) 50
PdiI GCCGGC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 4 cut(s) 57, 66, 82, 137
PpuMI RGGWCCY 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 5 cut(s) 14, 15, 26, 114, 123
PspPI GGNCC 4 cut(s) 13, 112, 122, 209
PspPPI RGGWCCY 1 cut(s) 13
PspXI VCTCGAGB 1 cut(s) 50
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 204
SacII CCGCGG 1 cut(s) 65
SaqAI TTAA 2 cut(s) 185, 189
SatI GCNGC 4 cut(s) 56, 65, 81, 136
Sau96I GGNCC 4 cut(s) 13, 112, 122, 209
ScrFI CCNGG 4 cut(s) 22, 93, 131, 132
SduI GDGCHC 1 cut(s) 8
SetI ASST 2 cut(s) 57, 151
SfaNI GCATC 1 cut(s) 208
Sfr274I CTCGAG 1 cut(s) 50
Sfr303I CCGCGG 1 cut(s) 65
SgrBI CCGCGG 1 cut(s) 65
SinI GGWCC 3 cut(s) 13, 112, 209
SlaI CTCGAG 1 cut(s) 50
SmaI CCCGGG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 1 cut(s) 186
SsiI CCGC 6 cut(s) 42, 62, 64, 73, 81, 215
StyD4I CCNGG 4 cut(s) 20, 91, 129, 130
TaaI ACNGT 2 cut(s) 112, 182
TaqI TCGA 1 cut(s) 51
TasI AATT 1 cut(s) 186
TauI GCSGC 2 cut(s) 67, 83
TfiI GAWTC 1 cut(s) 173
Tru1I TTAA 2 cut(s) 185, 189
Tru9I TTAA 2 cut(s) 185, 189
TseI GCWGC 2 cut(s) 55, 135
TspGWI ACGGA 1 cut(s) 177
TspMI CCCGGG 1 cut(s) 130
VpaK11BI GGWCC 3 cut(s) 13, 112, 209
XhoI CTCGAG 1 cut(s) 50
XmaI CCCGGG 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.