Rroxscaffold_12G00450640

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000012
OG0000104
Physical Location & Seq
Forward (+)
22100 .. 23673
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_12G00450640.1

Sequence Viewer

Length: 753 bp
ATGGTAGGCAGCATGCGCCGTCTCGGCGTGCTTCGGCATCTGCGTGCTCCTGGTAACGGCCTGCGGGCTCCCCATTCGGCCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGATTAAACCCGTAAGGCGCAAGGAAGCTAATGTGCGGGATCCCTTTGCGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGCGCGTCTCGCGCGAACTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGTGGTGGTTGACGGCAACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCATTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGAACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGCTCCTTCGGGGGCTTTCCCGGGCGTTCAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

26.87

Weight (kDa)

11.5

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 103
AatII GACGTC 1 cut(s) 309
AccB7I CCANNNNNTGG 1 cut(s) 396
AccBSI CCGCTC 1 cut(s) 640
AccI GTMKAC 1 cut(s) 712
AccII CGCG 6 cut(s) 219, 226, 228, 417, 631, 650
AccIII TCCGGA 2 cut(s) 299, 453
AclI AACGTT 1 cut(s) 288
AclWI GGATC 4 cut(s) 164, 177, 528, 541
AcoI YGGCCR 1 cut(s) 656
AcsI RAATTY 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 2 cut(s) 486, 727
AflIII ACRYGT 1 cut(s) 111
AgsI TTSAA 3 cut(s) 89, 448, 706
AjiI CACGTC 2 cut(s) 271, 413
AjnI CCWGG 2 cut(s) 49, 359
AluBI AGCT 2 cut(s) 158, 622
AluI AGCT 2 cut(s) 158, 622
Alw21I GWGCWC 3 cut(s) 49, 187, 407
Alw26I GTCTC 4 cut(s) 26, 226, 297, 509
Alw44I GTGCAC 1 cut(s) 183
AlwI GGATC 4 cut(s) 164, 177, 528, 541
AlwNI CAGNNNCTG 1 cut(s) 396
Ama87I CYCGRG 5 cut(s) 320, 544, 561, 617, 696
Aor13HI TCCGGA 2 cut(s) 299, 453
AoxI GGCC 5 cut(s) 58, 78, 316, 440, 656
ApaLI GTGCAC 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 9, 622
ApoI RAATTY 1 cut(s) 254
AspLEI GCGC 5 cut(s) 18, 150, 219, 228, 433
AspS9I GGNCC 7 cut(s) 79, 96, 316, 387, 441, 459, 580
AsuC2I CCSGG 6 cut(s) 266, 436, 479, 660, 697, 698
AsuHPI GGTGA 2 cut(s) 142, 620
AsuII TTCGAA 1 cut(s) 235
AvaI CYCGRG 5 cut(s) 320, 544, 561, 617, 696
AvaII GGWCC 4 cut(s) 96, 387, 459, 580
BaeGI GKGCMC 2 cut(s) 187, 575
BamHI GGATCC 2 cut(s) 169, 533
BanII GRGCYC 1 cut(s) 70
Bbv12I GWGCWC 3 cut(s) 49, 187, 407
BbvI GCAGC 2 cut(s) 21, 609
BceAI ACGGC 4 cut(s) 3, 73, 298, 384
BciT130I CCWGG 2 cut(s) 51, 361
BcnI CCSGG 6 cut(s) 266, 436, 479, 660, 697, 698
BcoDI GTCTC 4 cut(s) 26, 226, 297, 509
BglI GCCNNNNNGGC 2 cut(s) 24, 439
BisI GCNGC 6 cut(s) 10, 394, 440, 623, 632, 648
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 6 cut(s) 11, 395, 441, 624, 633, 649
Bme1390I CCNGG 8 cut(s) 51, 266, 361, 436, 479, 660, 697, 698
Bme18I GGWCC 4 cut(s) 96, 387, 459, 580
BmeT110I CYCGRG 5 cut(s) 320, 544, 561, 617, 696
BmgBI CACGTC 2 cut(s) 271, 413
BmgT120I GGNCC 7 cut(s) 79, 96, 316, 387, 441, 459, 580
BmiI GGNNCC 7 cut(s) 69, 171, 317, 388, 535, 581, 582
BmrFI CCNGG 8 cut(s) 51, 266, 361, 436, 479, 660, 697, 698
BmrI ACTGGG 2 cut(s) 578, 680
BmsI GCATC 2 cut(s) 46, 506
BmuI ACTGGG 2 cut(s) 578, 680
Bpu1102I GCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 235
BpuMI CCSGG 6 cut(s) 266, 436, 479, 660, 697, 698
BsaHI GRCGYC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 509
BsaWI WCCGGW 3 cut(s) 299, 425, 453
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bse118I RCCGGY 1 cut(s) 654
Bse1I ACTGG 3 cut(s) 584, 675, 727
BseAI TCCGGA 2 cut(s) 299, 453
BseBI CCWGG 2 cut(s) 51, 361
BseMII CTCAG 3 cut(s) 200, 386, 730
BseNI ACTGG 3 cut(s) 584, 675, 727
BseSI GKGCMC 2 cut(s) 187, 575
BseX3I CGGCCG 1 cut(s) 656
BseXI GCAGC 2 cut(s) 21, 609
BseYI CCCAGC 1 cut(s) 568
Bsh1236I CGCG 6 cut(s) 219, 226, 228, 417, 631, 650
Bsh1285I CGRYCG 3 cut(s) 195, 483, 659
BshFI GGCC 5 cut(s) 60, 80, 318, 442, 658
BsiEI CGRYCG 3 cut(s) 195, 483, 659
BsiHKAI GWGCWC 3 cut(s) 49, 187, 407
BsiHKCI CYCGRG 5 cut(s) 320, 544, 561, 617, 696
BslFI GGGAC 4 cut(s) 281, 566, 572, 677
BsmAI GTCTC 4 cut(s) 26, 226, 297, 509
BsmBI CGTCTC 3 cut(s) 26, 226, 297
BsmFI GGGAC 4 cut(s) 281, 566, 572, 677
BsnI GGCC 5 cut(s) 60, 80, 318, 442, 658
Bso31I GGTCTC 1 cut(s) 509
BsoBI CYCGRG 5 cut(s) 320, 544, 561, 617, 696
Bsp119I TTCGAA 1 cut(s) 235
Bsp1286I GDGCHC 5 cut(s) 49, 70, 187, 407, 575
Bsp13I TCCGGA 2 cut(s) 299, 453
Bsp143I GATC 3 cut(s) 169, 202, 533
Bsp1720I GCTNAGC 1 cut(s) 372
BspANI GGCC 5 cut(s) 60, 80, 318, 442, 658
BspCNI CTCAG 3 cut(s) 201, 385, 729
BspEI TCCGGA 2 cut(s) 299, 453
BspFNI CGCG 6 cut(s) 219, 226, 228, 417, 631, 650
BspLI GGNNCC 7 cut(s) 69, 171, 317, 388, 535, 581, 582
BspPI GGATC 4 cut(s) 164, 177, 528, 541
BspQI GCTCTTC 1 cut(s) 395
BspT104I TTCGAA 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 509
BsrBI CCGCTC 1 cut(s) 640
BsrFI RCCGGY 1 cut(s) 654
BsrI ACTGG 3 cut(s) 584, 675, 727
BssAI RCCGGY 1 cut(s) 654
BssMI GATC 3 cut(s) 169, 202, 533
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 99, 508
Bst2UI CCWGG 2 cut(s) 51, 361
Bst4CI ACNGT 2 cut(s) 710, 748
Bst6I CTCTTC 2 cut(s) 320, 395
BstACI GRCGYC 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 235
BstC8I GCNNGC 8 cut(s) 14, 29, 45, 62, 66, 352, 652, 656
BstDEI CTNAG 3 cut(s) 209, 372, 716
BstDSI CCRYGG 1 cut(s) 629
BstFNI CGCG 6 cut(s) 219, 226, 228, 417, 631, 650
BstHHI GCGC 5 cut(s) 18, 150, 219, 228, 433
BstKTI GATC 3 cut(s) 172, 205, 536
BstMAI GTCTC 4 cut(s) 26, 226, 297, 509
BstMBI GATC 3 cut(s) 169, 202, 533
BstMCI CGRYCG 3 cut(s) 195, 483, 659
BstNI CCWGG 2 cut(s) 51, 361
BstNSI RCATGY 2 cut(s) 16, 115
BstSCI CCNGG 8 cut(s) 49, 264, 359, 434, 477, 658, 695, 696
BstSLI GKGCMC 2 cut(s) 187, 575
BstUI CGCG 6 cut(s) 219, 226, 228, 417, 631, 650
BstV1I GCAGC 2 cut(s) 21, 609
BstX2I RGATCY 2 cut(s) 169, 533
BstYI RGATCY 2 cut(s) 169, 533
BstZI CGGCCG 1 cut(s) 656
BsuRI GGCC 5 cut(s) 60, 80, 318, 442, 658
BtgI CCRYGG 1 cut(s) 629
BtrI CACGTC 2 cut(s) 271, 413
Cac8I GCNNGC 8 cut(s) 14, 29, 45, 62, 66, 352, 652, 656
CaiI CAGNNNCTG 1 cut(s) 396
CfoI GCGC 5 cut(s) 18, 150, 219, 228, 433
Cfr10I RCCGGY 1 cut(s) 654
Cfr13I GGNCC 7 cut(s) 79, 96, 316, 387, 441, 459, 580
Cfr42I CCGCGG 1 cut(s) 632
Cfr9I CCCGGG 1 cut(s) 696
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 485, 726
CviAII CATG 2 cut(s) 13, 112
CviQI GTAC 2 cut(s) 485, 726
DdeI CTNAG 3 cut(s) 209, 372, 716
DpnI GATC 3 cut(s) 171, 204, 535
DpnII GATC 3 cut(s) 169, 202, 533
DrdI GACNNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 656
EagI CGGCCG 1 cut(s) 656
Eam1104I CTCTTC 2 cut(s) 320, 395
EarI CTCTTC 2 cut(s) 320, 395
EclXI CGGCCG 1 cut(s) 656
Eco130I CCWWGG 2 cut(s) 99, 508
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 509
Eco47I GGWCC 4 cut(s) 96, 387, 459, 580
Eco52I CGGCCG 1 cut(s) 656
Eco88I CYCGRG 5 cut(s) 320, 544, 561, 617, 696
EcoO109I RGGNCCY 2 cut(s) 316, 580
EcoRII CCWGG 2 cut(s) 49, 359
EcoT14I CCWWGG 2 cut(s) 99, 508
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 99, 508
Esp3I CGTCTC 3 cut(s) 26, 226, 297
FaeI CATG 2 cut(s) 16, 115
FaiI YATR 3 cut(s) 14, 113, 491
FaqI GGGAC 4 cut(s) 281, 566, 572, 677
FatI CATG 2 cut(s) 12, 111
FauI CCCGC 5 cut(s) 57, 159, 172, 305, 633
FblI GTMKAC 1 cut(s) 712
Fnu4HI GCNGC 6 cut(s) 10, 394, 440, 623, 632, 648
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 6 cut(s) 10, 394, 440, 623, 632, 648
GlaI GCGC 5 cut(s) 17, 149, 218, 227, 432
GluI GCNGC 6 cut(s) 10, 394, 440, 623, 632, 648
GsaI CCCAGC 1 cut(s) 572
HaeIII GGCC 5 cut(s) 60, 80, 318, 442, 658
HgaI GACGC 1 cut(s) 208
HhaI GCGC 5 cut(s) 18, 150, 219, 228, 433
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 2 cut(s) 16, 115
Hin6I GCGC 5 cut(s) 16, 148, 217, 226, 431
HinP1I GCGC 5 cut(s) 16, 148, 217, 226, 431
HincII GTYRAC 3 cut(s) 125, 280, 713
HindII GTYRAC 3 cut(s) 125, 280, 713
HinfI GANTC 6 cut(s) 104, 121, 243, 296, 714, 739
HphI GGTGA 2 cut(s) 142, 620
Hpy166II GTNNAC 5 cut(s) 125, 185, 280, 611, 713
Hpy188I TCNGA 4 cut(s) 109, 210, 719, 744
Hpy188III TCNNGA 7 cut(s) 86, 259, 300, 322, 454, 546, 589
Hpy8I GTNNAC 5 cut(s) 125, 185, 280, 611, 713
Hpy99I CGWCG 3 cut(s) 311, 417, 601
HpyAV CCTTC 2 cut(s) 207, 692
HpyCH4III ACNGT 2 cut(s) 710, 748
HpyCH4IV ACGT 4 cut(s) 270, 288, 306, 412
HpyCH4V TGCA 1 cut(s) 185
HpyF3I CTNAG 3 cut(s) 209, 372, 716
HpySE526I ACGT 4 cut(s) 270, 288, 306, 412
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 2 cut(s) 16, 115
HspAI GCGC 5 cut(s) 16, 148, 217, 226, 431
KflI GGGWCCC 1 cut(s) 580
Kpn2I TCCGGA 2 cut(s) 299, 453
KroI GCCGGC 1 cut(s) 654
KroNI GCCGGC 1 cut(s) 656
KspI CCGCGG 1 cut(s) 632
Kzo9I GATC 3 cut(s) 169, 202, 533
LguI GCTCTTC 1 cut(s) 395
LmnI GCTCC 3 cut(s) 52, 73, 684
Lsp1109I GCAGC 2 cut(s) 21, 609
LweI GCATC 2 cut(s) 46, 506
MaeII ACGT 4 cut(s) 270, 288, 306, 412
MaeIII GTNAC 2 cut(s) 53, 538
MalI GATC 3 cut(s) 171, 204, 535
MbiI CCGCTC 1 cut(s) 640
MboI GATC 3 cut(s) 169, 202, 533
MboII GAAGA 3 cut(s) 197, 337, 412
MflI RGATCY 2 cut(s) 169, 533
MhlI GDGCHC 5 cut(s) 49, 70, 187, 407, 575
MluCI AATT 1 cut(s) 254
MlyI GAGTC 4 cut(s) 113, 130, 305, 708
MnlI CCTC 5 cut(s) 329, 373, 450, 553, 557
MroI TCCGGA 2 cut(s) 299, 453
MroNI GCCGGC 1 cut(s) 654
MseI TTAA 4 cut(s) 135, 251, 344, 751
MslI CAYNNNNRTG 1 cut(s) 42
MspA1I CMGCKG 2 cut(s) 393, 631
MspR9I CCNGG 8 cut(s) 51, 266, 361, 436, 479, 660, 697, 698
MvaI CCWGG 2 cut(s) 51, 361
MvnI CGCG 6 cut(s) 219, 226, 228, 417, 631, 650
NaeI GCCGGC 1 cut(s) 656
NciI CCSGG 6 cut(s) 266, 436, 479, 660, 697, 698
NdeII GATC 3 cut(s) 169, 202, 533
NgoMIV GCCGGC 1 cut(s) 654
NlaIII CATG 2 cut(s) 16, 115
NlaIV GGNNCC 7 cut(s) 69, 171, 317, 388, 535, 581, 582
NspI RCATGY 2 cut(s) 16, 115
NspV TTCGAA 1 cut(s) 235
PaeI GCATGC 1 cut(s) 16
PaeR7I CTCGAG 2 cut(s) 561, 617
PasI CCCWGGG 1 cut(s) 360
PciI ACATGT 1 cut(s) 111
PciSI GCTCTTC 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PdiI GCCGGC 1 cut(s) 656
PfeI GAWTC 2 cut(s) 243, 739
PflMI CCANNNNNTGG 1 cut(s) 396
PkrI GCNGC 6 cut(s) 11, 395, 441, 624, 633, 649
PleI GAGTC 4 cut(s) 112, 129, 304, 708
PpsI GAGTC 4 cut(s) 112, 129, 304, 708
PpuMI RGGWCCY 1 cut(s) 580
PscI ACATGT 1 cut(s) 111
Psp1406I AACGTT 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 49, 359
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 2 cut(s) 49, 359
PspN4I GGNNCC 7 cut(s) 69, 171, 317, 388, 535, 581, 582
PspPI GGNCC 7 cut(s) 79, 96, 316, 387, 441, 459, 580
PspPPI RGGWCCY 1 cut(s) 580
PspXI VCTCGAGB 2 cut(s) 561, 617
PstNI CAGNNNCTG 1 cut(s) 396
PsuI RGATCY 2 cut(s) 169, 533
RsaI GTAC 2 cut(s) 486, 727
RsaNI GTAC 2 cut(s) 485, 726
RseI CAYNNNNRTG 1 cut(s) 42
SacII CCGCGG 1 cut(s) 632
SalI GTCGAC 1 cut(s) 711
SapI GCTCTTC 1 cut(s) 395
SaqAI TTAA 4 cut(s) 135, 251, 344, 751
SatI GCNGC 6 cut(s) 10, 394, 440, 623, 632, 648
Sau3AI GATC 3 cut(s) 169, 202, 533
Sau96I GGNCC 7 cut(s) 79, 96, 316, 387, 441, 459, 580
SchI GAGTC 4 cut(s) 113, 130, 305, 708
ScrFI CCNGG 8 cut(s) 51, 266, 361, 436, 479, 660, 697, 698
SduI GDGCHC 5 cut(s) 49, 70, 187, 407, 575
SfaNI GCATC 2 cut(s) 46, 506
Sfr274I CTCGAG 2 cut(s) 561, 617
Sfr303I CCGCGG 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 632
SinI GGWCC 4 cut(s) 96, 387, 459, 580
SlaI CTCGAG 2 cut(s) 561, 617
SmaI CCCGGG 1 cut(s) 698
SmiMI CAYNNNNRTG 1 cut(s) 42
SmlI CTYRAG 2 cut(s) 561, 617
SmoI CTYRAG 2 cut(s) 561, 617
SphI GCATGC 1 cut(s) 16
Sse9I AATT 1 cut(s) 254
StyD4I CCNGG 8 cut(s) 49, 264, 359, 434, 477, 658, 695, 696
StyI CCWWGG 2 cut(s) 99, 508
TaaI ACNGT 2 cut(s) 710, 748
TaiI ACGT 4 cut(s) 273, 291, 309, 415
TaqI TCGA 4 cut(s) 235, 562, 618, 712
TaqII GACCGA 2 cut(s) 209, 476
TasI AATT 1 cut(s) 254
TauI GCSGC 4 cut(s) 396, 442, 634, 650
TfiI GAWTC 2 cut(s) 243, 739
Tru1I TTAA 4 cut(s) 135, 251, 344, 751
Tru9I TTAA 4 cut(s) 135, 251, 344, 751
TseI GCWGC 2 cut(s) 9, 622
TspGWI ACGGA 4 cut(s) 109, 526, 681, 743
TspMI CCCGGG 1 cut(s) 696
Van91I CCANNNNNTGG 1 cut(s) 396
VneI GTGCAC 1 cut(s) 183
VpaK11BI GGWCC 4 cut(s) 96, 387, 459, 580
XapI RAATTY 1 cut(s) 254
XceI RCATGY 2 cut(s) 16, 115
XhoI CTCGAG 2 cut(s) 561, 617
XmaI CCCGGG 1 cut(s) 696
XmiI GTMKAC 1 cut(s) 712
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.