Rroxscaffold_65G00445350

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000065
OG0000104
Physical Location & Seq
Reverse (-)
63390 .. 63798
409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_65G00445350.1

Sequence Viewer

Length: 321 bp
ATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCGAGGGCTGGGCACGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGAACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGACGGACTGGGAACGCTCCTTCGGGGGCTTTCCCGGGCGTTCAACAGTCGACTCAGAACTGTGCTCGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.6

Weight (kDa)

10.12

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 142
AccI GTMKAC 1 cut(s) 213
AccII CGCG 3 cut(s) 133, 152, 262
AciI CCGC 6 cut(s) 131, 133, 142, 150, 262, 273
AclWI GGATC 2 cut(s) 31, 44
AcoI YGGCCR 1 cut(s) 158
AcsI RAATTY 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 156, 262
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 2 cut(s) 207, 253
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
Alw21I GWGCWC 1 cut(s) 230
AlwI GGATC 2 cut(s) 31, 44
Ama87I CYCGRG 4 cut(s) 47, 64, 119, 197
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 124
ApoI RAATTY 1 cut(s) 248
ArsI GACNNNNNNTTYG 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 1 cut(s) 82
AsuC2I CCSGG 3 cut(s) 162, 198, 199
AsuHPI GGTGA 1 cut(s) 122
AvaI CYCGRG 4 cut(s) 47, 64, 119, 197
AvaII GGWCC 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 78
BamHI GGATCC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 1 cut(s) 111
BceAI ACGGC 1 cut(s) 286
BcnI CCSGG 3 cut(s) 162, 198, 199
BfrI CTTAAG 1 cut(s) 293
BisI GCNGC 3 cut(s) 125, 134, 150
BlsI GCNGC 3 cut(s) 126, 135, 151
Bme1390I CCNGG 3 cut(s) 162, 198, 199
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 4 cut(s) 47, 64, 119, 197
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 3 cut(s) 38, 83, 84
BmrFI CCNGG 3 cut(s) 162, 198, 199
BmrI ACTGGG 2 cut(s) 80, 181
BmuI ACTGGG 2 cut(s) 80, 181
BpuMI CCSGG 3 cut(s) 162, 198, 199
BsaJI CCNNGG 4 cut(s) 46, 131, 161, 197
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 262
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 86, 176
BseDI CCNNGG 4 cut(s) 46, 131, 161, 197
BseLI CCNNNNNNNGG 2 cut(s) 156, 262
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 86, 176
BseSI GKGCMC 1 cut(s) 78
BseX3I CGGCCG 1 cut(s) 158
BseXI GCAGC 1 cut(s) 111
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 3 cut(s) 133, 152, 262
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 1 cut(s) 160
BsiEI CGRYCG 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 230
BsiHKCI CYCGRG 4 cut(s) 47, 64, 119, 197
BsiSI CCGG 3 cut(s) 157, 161, 198
BslFI GGGAC 3 cut(s) 68, 74, 178
BslI CCNNNNNNNGG 2 cut(s) 156, 262
BsmFI GGGAC 3 cut(s) 68, 74, 178
BsnI GGCC 1 cut(s) 160
BsoBI CYCGRG 4 cut(s) 47, 64, 119, 197
Bsp1286I GDGCHC 2 cut(s) 78, 230
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 6 cut(s) 131, 133, 142, 150, 262, 273
BspANI GGCC 1 cut(s) 160
BspCNI CTCAG 1 cut(s) 230
BspFNI CGCG 3 cut(s) 133, 152, 262
BspLI GGNNCC 3 cut(s) 38, 83, 84
BspPI GGATC 2 cut(s) 31, 44
BspTI CTTAAG 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 156
BsrI ACTGG 2 cut(s) 86, 176
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 4 cut(s) 46, 131, 161, 197
BssMI GATC 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 211, 225
BstAFI CTTAAG 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 154, 158
BstDEI CTNAG 1 cut(s) 217
BstDSI CCRYGG 1 cut(s) 131
BstFNI CGCG 3 cut(s) 133, 152, 262
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 4 cut(s) 124, 130, 133, 139
BstSCI CCNGG 3 cut(s) 160, 196, 197
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 3 cut(s) 133, 152, 262
BstV1I GCAGC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BstZI CGGCCG 1 cut(s) 158
BsuRI GGCC 1 cut(s) 160
BtgI CCRYGG 1 cut(s) 131
Cac8I GCNNGC 2 cut(s) 154, 158
CfoI GCGC 1 cut(s) 262
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 82
Cfr42I CCGCGG 1 cut(s) 134
Cfr9I CCCGGG 1 cut(s) 197
CviJI RGCY 7 cut(s) 63, 71, 104, 124, 160, 192, 302
CviKI_1 RGCY 7 cut(s) 63, 71, 104, 124, 160, 192, 302
DdeI CTNAG 1 cut(s) 217
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 1 cut(s) 158
EagI CGGCCG 1 cut(s) 158
EclXI CGGCCG 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 158
Eco88I CYCGRG 4 cut(s) 47, 64, 119, 197
EcoO109I RGGNCCY 1 cut(s) 82
FaiI YATR 1 cut(s) 285
FaqI GGGAC 3 cut(s) 68, 74, 178
FauI CCCGC 3 cut(s) 135, 255, 266
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 3 cut(s) 125, 134, 150
Fsp4HI GCNGC 3 cut(s) 125, 134, 150
GlaI GCGC 1 cut(s) 261
GluI GCNGC 3 cut(s) 125, 134, 150
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 1 cut(s) 160
HapII CCGG 3 cut(s) 157, 161, 198
HhaI GCGC 1 cut(s) 262
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 2 cut(s) 215, 287
HpaII CCGG 3 cut(s) 157, 161, 198
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 3 cut(s) 113, 214, 268
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 2 cut(s) 49, 91
Hpy8I GTNNAC 3 cut(s) 113, 214, 268
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 2 cut(s) 211, 225
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 130, 133, 139
HpyF3I CTNAG 1 cut(s) 217
HspAI GCGC 1 cut(s) 260
KflI GGGWCCC 1 cut(s) 82
KroI GCCGGC 1 cut(s) 156
KroNI GCCGGC 1 cut(s) 158
KspI CCGCGG 1 cut(s) 134
Kzo9I GATC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 6 cut(s) 57, 99, 157, 170, 174, 211
Lsp1109I GCAGC 1 cut(s) 111
MaeIII GTNAC 2 cut(s) 41, 278
MalI GATC 1 cut(s) 38
MbiI CCGCTC 1 cut(s) 142
MboI GATC 1 cut(s) 36
MboII GAAGA 1 cut(s) 256
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 2 cut(s) 78, 230
MluCI AATT 1 cut(s) 248
MlyI GAGTC 2 cut(s) 209, 281
MnlI CCTC 3 cut(s) 56, 60, 320
MroNI GCCGGC 1 cut(s) 156
MroXI GAANNNNTTC 1 cut(s) 248
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 133
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 3 cut(s) 157, 161, 198
MspR9I CCNGG 3 cut(s) 162, 198, 199
MvnI CGCG 3 cut(s) 133, 152, 262
MwoI GCNNNNNNNGC 4 cut(s) 124, 130, 133, 139
NaeI GCCGGC 1 cut(s) 158
NciI CCSGG 3 cut(s) 162, 198, 199
NdeII GATC 1 cut(s) 36
NgoMIV GCCGGC 1 cut(s) 156
NlaIV GGNNCC 3 cut(s) 38, 83, 84
PaeR7I CTCGAG 2 cut(s) 64, 119
PdiI GCCGGC 1 cut(s) 158
PdmI GAANNNNTTC 1 cut(s) 248
PkrI GCNGC 3 cut(s) 126, 135, 151
PleI GAGTC 2 cut(s) 209, 281
PpsI GAGTC 2 cut(s) 209, 281
PpuMI RGGWCCY 1 cut(s) 82
Psp5II RGGWCCY 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 71
PspN4I GGNNCC 3 cut(s) 38, 83, 84
PspPI GGNCC 1 cut(s) 82
PspPPI RGGWCCY 1 cut(s) 82
PspXI VCTCGAGB 2 cut(s) 64, 119
PsuI RGATCY 1 cut(s) 36
SacII CCGCGG 1 cut(s) 134
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 125, 134, 150
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 2 cut(s) 209, 281
ScrFI CCNGG 3 cut(s) 162, 198, 199
SduI GDGCHC 2 cut(s) 78, 230
SetI ASST 3 cut(s) 48, 126, 300
Sfr274I CTCGAG 2 cut(s) 64, 119
Sfr303I CCGCGG 1 cut(s) 134
SgrBI CCGCGG 1 cut(s) 134
SinI GGWCC 1 cut(s) 82
SlaI CTCGAG 2 cut(s) 64, 119
SmaI CCCGGG 1 cut(s) 199
SmlI CTYRAG 3 cut(s) 64, 119, 293
SmoI CTYRAG 3 cut(s) 64, 119, 293
Sse9I AATT 1 cut(s) 248
SsiI CCGC 6 cut(s) 131, 133, 142, 150, 262, 273
StyD4I CCNGG 3 cut(s) 160, 196, 197
TaaI ACNGT 2 cut(s) 211, 225
TaqI TCGA 4 cut(s) 65, 120, 213, 230
TasI AATT 1 cut(s) 248
TauI GCSGC 2 cut(s) 136, 152
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 1 cut(s) 124
TspGWI ACGGA 2 cut(s) 29, 182
TspMI CCCGGG 1 cut(s) 197
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 1 cut(s) 248
XhoI CTCGAG 2 cut(s) 64, 119
XmaI CCCGGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.