Rroxscaffold_21G00439660

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000021
OG0000104
Physical Location & Seq
Forward (+)
149028 .. 149848
821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_21G00439660.1

Sequence Viewer

Length: 498 bp
ATGGCGCTTAAGCGCGTGACCTATACCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGTGAGCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTCTCCAAGGTGAACAGCCTCTGGTCAATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTGCGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGAACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGCTCCTTCGGGGGCTTTCCCGGGCGTTCAACGGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.34

Weight (kDa)

11.41

Isoelectric Point (pI)

55.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 110, 381
AccI GTMKAC 1 cut(s) 453
AccII CGCG 4 cut(s) 15, 70, 372, 391
AciI CCGC 8 cut(s) 70, 73, 105, 110, 370, 372, 381, 389
AclWI GGATC 2 cut(s) 268, 281
AcoI YGGCCR 3 cut(s) 28, 111, 397
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 5 cut(s) 33, 175, 395, 402, 406
AflII CTTAAG 1 cut(s) 8
AgsI TTSAA 3 cut(s) 150, 164, 447
AjnI CCWGG 2 cut(s) 45, 87
AluBI AGCT 1 cut(s) 363
AluI AGCT 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 2 cut(s) 268, 281
AlwNI CAGNNNCTG 1 cut(s) 231
Ama87I CYCGRG 3 cut(s) 284, 358, 437
AoxI GGCC 5 cut(s) 28, 48, 111, 166, 397
ApeKI GCWGC 3 cut(s) 79, 300, 363
AspLEI GCGC 3 cut(s) 7, 15, 70
AspS9I GGNCC 2 cut(s) 49, 321
AsuC2I CCSGG 4 cut(s) 102, 401, 438, 439
AsuHPI GGTGA 2 cut(s) 232, 361
AvaI CYCGRG 3 cut(s) 284, 358, 437
AvaII GGWCC 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 316
BamHI GGATCC 1 cut(s) 273
BbvI GCAGC 3 cut(s) 66, 287, 350
BceAI ACGGC 3 cut(s) 15, 98, 211
BciT130I CCWGG 2 cut(s) 47, 89
BcnI CCSGG 4 cut(s) 102, 401, 438, 439
BcoDI GTCTC 1 cut(s) 217
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 36
BglII AGATCT 1 cut(s) 124
BisI GCNGC 7 cut(s) 71, 80, 111, 301, 364, 373, 389
BlsI GCNGC 7 cut(s) 72, 81, 112, 302, 365, 374, 390
Bme1390I CCNGG 6 cut(s) 47, 89, 102, 401, 438, 439
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 3 cut(s) 284, 358, 437
BmgT120I GGNCC 2 cut(s) 49, 321
BmiI GGNNCC 5 cut(s) 51, 185, 275, 322, 323
BmrFI CCNGG 6 cut(s) 47, 89, 102, 401, 438, 439
BmrI ACTGGG 2 cut(s) 319, 421
BmuI ACTGGG 2 cut(s) 319, 421
BpuMI CCSGG 4 cut(s) 102, 401, 438, 439
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 6 cut(s) 88, 216, 283, 370, 400, 437
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 175, 395, 402, 406
Bse118I RCCGGY 2 cut(s) 26, 395
Bse1I ACTGG 3 cut(s) 325, 416, 468
BseBI CCWGG 2 cut(s) 47, 89
BseDI CCNNGG 6 cut(s) 88, 216, 283, 370, 400, 437
BseLI CCNNNNNNNGG 5 cut(s) 33, 175, 395, 402, 406
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 3 cut(s) 325, 416, 468
BseSI GKGCMC 1 cut(s) 316
BseX3I CGGCCG 3 cut(s) 28, 111, 397
BseXI GCAGC 3 cut(s) 66, 287, 350
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 4 cut(s) 15, 70, 372, 391
Bsh1285I CGRYCG 5 cut(s) 31, 77, 114, 400, 453
BshFI GGCC 5 cut(s) 30, 50, 113, 168, 399
BsiEI CGRYCG 5 cut(s) 31, 77, 114, 400, 453
BsiHKCI CYCGRG 3 cut(s) 284, 358, 437
BsiSI CCGG 5 cut(s) 27, 101, 396, 400, 438
BslFI GGGAC 3 cut(s) 307, 313, 418
BslI CCNNNNNNNGG 5 cut(s) 33, 175, 395, 402, 406
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 3 cut(s) 307, 313, 418
BsnI GGCC 5 cut(s) 30, 50, 113, 168, 399
Bso31I GGTCTC 1 cut(s) 217
BsoBI CYCGRG 3 cut(s) 284, 358, 437
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 2 cut(s) 124, 273
BspACI CCGC 8 cut(s) 70, 73, 105, 110, 370, 372, 381, 389
BspANI GGCC 5 cut(s) 30, 50, 113, 168, 399
BspCNI CTCAG 1 cut(s) 470
BspFNI CGCG 4 cut(s) 15, 70, 372, 391
BspLI GGNNCC 5 cut(s) 51, 185, 275, 322, 323
BspPI GGATC 2 cut(s) 268, 281
BspTI CTTAAG 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 217
BsrBI CCGCTC 2 cut(s) 110, 381
BsrFI RCCGGY 2 cut(s) 26, 395
BsrI ACTGG 3 cut(s) 325, 416, 468
BssAI RCCGGY 2 cut(s) 26, 395
BssECI CCNNGG 6 cut(s) 88, 216, 283, 370, 400, 437
BssMI GATC 2 cut(s) 124, 273
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 47, 89
Bst4CI ACNGT 2 cut(s) 451, 489
Bst6I CTCTTC 1 cut(s) 166
BstAFI CTTAAG 1 cut(s) 8
BstC8I GCNNGC 2 cut(s) 393, 397
BstDEI CTNAG 1 cut(s) 457
BstDSI CCRYGG 1 cut(s) 370
BstFNI CGCG 4 cut(s) 15, 70, 372, 391
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 15, 70
BstKTI GATC 2 cut(s) 127, 276
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 2 cut(s) 124, 273
BstMCI CGRYCG 5 cut(s) 31, 77, 114, 400, 453
BstMWI GCNNNNNNNGC 9 cut(s) 36, 76, 79, 119, 306, 363, 369, 372, 378
BstNI CCWGG 2 cut(s) 47, 89
BstSCI CCNGG 6 cut(s) 45, 87, 100, 399, 436, 437
BstSLI GKGCMC 1 cut(s) 316
BstUI CGCG 4 cut(s) 15, 70, 372, 391
BstV1I GCAGC 3 cut(s) 66, 287, 350
BstX2I RGATCY 2 cut(s) 124, 273
BstYI RGATCY 2 cut(s) 124, 273
BstZI CGGCCG 3 cut(s) 28, 111, 397
BsuRI GGCC 5 cut(s) 30, 50, 113, 168, 399
BtgI CCRYGG 1 cut(s) 370
Cac8I GCNNGC 2 cut(s) 393, 397
CaiI CAGNNNCTG 1 cut(s) 231
CfoI GCGC 3 cut(s) 7, 15, 70
Cfr10I RCCGGY 2 cut(s) 26, 395
Cfr13I GGNCC 2 cut(s) 49, 321
Cfr42I CCGCGG 1 cut(s) 373
Cfr9I CCCGGG 1 cut(s) 437
Csp6I GTAC 1 cut(s) 467
CviAII CATG 2 cut(s) 188, 207
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 1 cut(s) 457
DpnI GATC 2 cut(s) 126, 275
DpnII GATC 2 cut(s) 124, 273
EaeI YGGCCR 3 cut(s) 28, 111, 397
EagI CGGCCG 3 cut(s) 28, 111, 397
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
EciI GGCGGA 1 cut(s) 120
EclXI CGGCCG 3 cut(s) 28, 111, 397
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 321
Eco52I CGGCCG 3 cut(s) 28, 111, 397
Eco88I CYCGRG 3 cut(s) 284, 358, 437
EcoO109I RGGNCCY 2 cut(s) 49, 321
EcoRII CCWGG 2 cut(s) 45, 87
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 191, 210
FaiI YATR 3 cut(s) 24, 189, 208
FaqI GGGAC 3 cut(s) 307, 313, 418
FatI CATG 2 cut(s) 187, 206
FauI CCCGC 1 cut(s) 374
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 7 cut(s) 71, 80, 111, 301, 364, 373, 389
Fsp4HI GCNGC 7 cut(s) 71, 80, 111, 301, 364, 373, 389
GlaI GCGC 3 cut(s) 6, 14, 69
GluI GCNGC 7 cut(s) 71, 80, 111, 301, 364, 373, 389
GsaI CCCAGC 1 cut(s) 313
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 30, 50, 113, 168, 399
HapII CCGG 5 cut(s) 27, 101, 396, 400, 438
HhaI GCGC 3 cut(s) 7, 15, 70
Hin1II CATG 2 cut(s) 191, 210
Hin6I GCGC 3 cut(s) 5, 13, 68
HinP1I GCGC 3 cut(s) 5, 13, 68
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 2 cut(s) 455, 480
HpaII CCGG 5 cut(s) 27, 101, 396, 400, 438
HphI GGTGA 2 cut(s) 232, 361
Hpy166II GTNNAC 4 cut(s) 193, 223, 352, 454
Hpy188I TCNGA 2 cut(s) 460, 485
Hpy188III TCNNGA 2 cut(s) 286, 330
Hpy8I GTNNAC 4 cut(s) 193, 223, 352, 454
Hpy99I CGWCG 3 cut(s) 36, 119, 342
HpyAV CCTTC 2 cut(s) 158, 433
HpyCH4III ACNGT 2 cut(s) 451, 489
HpyCH4V TGCA 3 cut(s) 82, 122, 204
HpyF10VI GCNNNNNNNGC 9 cut(s) 36, 76, 79, 119, 306, 363, 369, 372, 378
HpyF3I CTNAG 1 cut(s) 457
Hsp92II CATG 2 cut(s) 191, 210
HspAI GCGC 3 cut(s) 5, 13, 68
KflI GGGWCCC 1 cut(s) 321
KroI GCCGGC 1 cut(s) 395
KroNI GCCGGC 1 cut(s) 397
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 2 cut(s) 124, 273
LmnI GCTCC 2 cut(s) 107, 425
Lsp1109I GCAGC 3 cut(s) 66, 287, 350
MaeIII GTNAC 2 cut(s) 16, 278
MalI GATC 2 cut(s) 126, 275
MbiI CCGCTC 2 cut(s) 110, 381
MboI GATC 2 cut(s) 124, 273
MboII GAAGA 1 cut(s) 183
MflI RGATCY 2 cut(s) 124, 273
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 1 cut(s) 493
MlyI GAGTC 1 cut(s) 449
MnlI CCTC 5 cut(s) 57, 167, 239, 293, 298
MroNI GCCGGC 1 cut(s) 395
MseI TTAA 3 cut(s) 9, 492, 496
MspA1I CMGCKG 1 cut(s) 372
MspCI CTTAAG 1 cut(s) 8
MspI CCGG 5 cut(s) 27, 101, 396, 400, 438
MspR9I CCNGG 6 cut(s) 47, 89, 102, 401, 438, 439
MvaI CCWGG 2 cut(s) 47, 89
MvnI CGCG 4 cut(s) 15, 70, 372, 391
MwoI GCNNNNNNNGC 9 cut(s) 36, 76, 79, 119, 306, 363, 369, 372, 378
NaeI GCCGGC 1 cut(s) 397
NciI CCSGG 4 cut(s) 102, 401, 438, 439
NdeII GATC 2 cut(s) 124, 273
NgoMIV GCCGGC 1 cut(s) 395
NlaIII CATG 2 cut(s) 191, 210
NlaIV GGNNCC 5 cut(s) 51, 185, 275, 322, 323
NmuCI GTSAC 1 cut(s) 16
PacI TTAATTAA 1 cut(s) 496
PaeR7I CTCGAG 1 cut(s) 358
PdiI GCCGGC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 480
PkrI GCNGC 7 cut(s) 72, 81, 112, 302, 365, 374, 390
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 321
Psp6I CCWGG 2 cut(s) 45, 87
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 2 cut(s) 45, 87
PspN4I GGNNCC 5 cut(s) 51, 185, 275, 322, 323
PspPI GGNCC 2 cut(s) 49, 321
PspPPI RGGWCCY 1 cut(s) 321
PspXI VCTCGAGB 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 231
PsuI RGATCY 2 cut(s) 124, 273
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SacII CCGCGG 1 cut(s) 373
SalI GTCGAC 1 cut(s) 452
SaqAI TTAA 3 cut(s) 9, 492, 496
SatI GCNGC 7 cut(s) 71, 80, 111, 301, 364, 373, 389
Sau3AI GATC 2 cut(s) 124, 273
Sau96I GGNCC 2 cut(s) 49, 321
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 6 cut(s) 47, 89, 102, 401, 438, 439
SduI GDGCHC 1 cut(s) 316
SetI ASST 6 cut(s) 23, 90, 185, 222, 285, 365
Sfr274I CTCGAG 1 cut(s) 358
Sfr303I CCGCGG 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 373
SinI GGWCC 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 358
SmaI CCCGGG 1 cut(s) 439
SmlI CTYRAG 2 cut(s) 8, 358
SmoI CTYRAG 2 cut(s) 8, 358
Sse9I AATT 1 cut(s) 493
SsiI CCGC 8 cut(s) 70, 73, 105, 110, 370, 372, 381, 389
SspI AATATT 1 cut(s) 146
StyD4I CCNGG 6 cut(s) 45, 87, 100, 399, 436, 437
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 2 cut(s) 451, 489
TaqI TCGA 2 cut(s) 359, 453
TasI AATT 1 cut(s) 493
TauI GCSGC 4 cut(s) 73, 113, 375, 391
TfiI GAWTC 1 cut(s) 480
Tru1I TTAA 3 cut(s) 9, 492, 496
Tru9I TTAA 3 cut(s) 9, 492, 496
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 3 cut(s) 79, 300, 363
Tsp45I GTSAC 1 cut(s) 16
TspGWI ACGGA 3 cut(s) 266, 422, 484
TspMI CCCGGG 1 cut(s) 437
Vha464I CTTAAG 1 cut(s) 8
VpaK11BI GGWCC 1 cut(s) 321
XhoI CTCGAG 1 cut(s) 358
XmaI CCCGGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.