Rroxscaffold_22G00439990

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000022
OG0000104
Physical Location & Seq
Forward (+)
129259 .. 130020
762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_22G00439990.1

Sequence Viewer

Length: 354 bp
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGTGAGCCCGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGATTGGCTCGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGAACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGACGGACTGGGAACGCTCCTTCGGGGCTTTCCCGGGCGTTCAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

12.66

Weight (kDa)

11.56

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 56, 243
AccI GTMKAC 1 cut(s) 313
AccII CGCG 3 cut(s) 16, 234, 253
AciI CCGC 8 cut(s) 16, 19, 51, 56, 232, 234, 243, 251
AcoI YGGCCR 2 cut(s) 57, 259
AfaI GTAC 1 cut(s) 328
AfiI CCNNNNNNNGG 2 cut(s) 121, 257
AgsI TTSAA 3 cut(s) 96, 110, 307
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 1 cut(s) 225
AluI AGCT 1 cut(s) 225
Ama87I CYCGRG 3 cut(s) 164, 220, 297
AoxI GGCC 3 cut(s) 57, 112, 259
ApeKI GCWGC 2 cut(s) 25, 225
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 183
AsuC2I CCSGG 4 cut(s) 48, 263, 298, 299
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 3 cut(s) 164, 220, 297
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 178
BanII GRGCYC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 12, 212
BceAI ACGGC 2 cut(s) 44, 157
BciT130I CCWGG 1 cut(s) 35
BcnI CCSGG 4 cut(s) 48, 263, 298, 299
BglII AGATCT 1 cut(s) 70
BisI GCNGC 6 cut(s) 17, 26, 57, 226, 235, 251
BlsI GCNGC 6 cut(s) 18, 27, 58, 227, 236, 252
Bme1390I CCNGG 5 cut(s) 35, 48, 263, 298, 299
Bme18I GGWCC 1 cut(s) 183
BmeT110I CYCGRG 3 cut(s) 164, 220, 297
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 3 cut(s) 131, 184, 185
BmrFI CCNGG 5 cut(s) 35, 48, 263, 298, 299
BmrI ACTGGG 2 cut(s) 181, 282
BmuI ACTGGG 2 cut(s) 181, 282
BpuMI CCSGG 4 cut(s) 48, 263, 298, 299
BsaJI CCNNGG 4 cut(s) 34, 232, 262, 297
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 257
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 3 cut(s) 187, 277, 328
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 4 cut(s) 34, 232, 262, 297
BseLI CCNNNNNNNGG 2 cut(s) 121, 257
BseMII CTCAG 1 cut(s) 331
BseNI ACTGG 3 cut(s) 187, 277, 328
BseSI GKGCMC 1 cut(s) 178
BseX3I CGGCCG 2 cut(s) 57, 259
BseXI GCAGC 2 cut(s) 12, 212
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 3 cut(s) 16, 234, 253
Bsh1285I CGRYCG 3 cut(s) 23, 60, 262
BshFI GGCC 3 cut(s) 59, 114, 261
BsiEI CGRYCG 3 cut(s) 23, 60, 262
BsiHKCI CYCGRG 3 cut(s) 164, 220, 297
BsiSI CCGG 4 cut(s) 48, 258, 262, 298
BslFI GGGAC 3 cut(s) 169, 175, 279
BslI CCNNNNNNNGG 2 cut(s) 121, 257
BsmFI GGGAC 3 cut(s) 169, 175, 279
BsnI GGCC 3 cut(s) 59, 114, 261
BsoBI CYCGRG 3 cut(s) 164, 220, 297
Bsp1286I GDGCHC 2 cut(s) 48, 178
Bsp143I GATC 1 cut(s) 70
BspACI CCGC 8 cut(s) 16, 19, 51, 56, 232, 234, 243, 251
BspANI GGCC 3 cut(s) 59, 114, 261
BspCNI CTCAG 1 cut(s) 330
BspFNI CGCG 3 cut(s) 16, 234, 253
BspLI GGNNCC 3 cut(s) 131, 184, 185
BsrBI CCGCTC 2 cut(s) 56, 243
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 3 cut(s) 187, 277, 328
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 4 cut(s) 34, 232, 262, 297
BssMI GATC 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 311, 349
Bst6I CTCTTC 1 cut(s) 112
BstC8I GCNNGC 2 cut(s) 255, 259
BstDEI CTNAG 1 cut(s) 317
BstDSI CCRYGG 1 cut(s) 232
BstFNI CGCG 3 cut(s) 16, 234, 253
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 1 cut(s) 73
BstMBI GATC 1 cut(s) 70
BstMCI CGRYCG 3 cut(s) 23, 60, 262
BstMWI GCNNNNNNNGC 7 cut(s) 22, 25, 65, 225, 231, 234, 240
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 5 cut(s) 33, 46, 261, 296, 297
BstSLI GKGCMC 1 cut(s) 178
BstUI CGCG 3 cut(s) 16, 234, 253
BstV1I GCAGC 2 cut(s) 12, 212
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BstZI CGGCCG 2 cut(s) 57, 259
BsuRI GGCC 3 cut(s) 59, 114, 261
BtgI CCRYGG 1 cut(s) 232
Cac8I GCNNGC 2 cut(s) 255, 259
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 183
Cfr42I CCGCGG 1 cut(s) 235
Cfr9I CCCGGG 1 cut(s) 297
Csp6I GTAC 1 cut(s) 327
CviAII CATG 2 cut(s) 134, 153
CviJI RGCY 9 cut(s) 46, 59, 114, 163, 171, 205, 225, 261, 292
CviKI_1 RGCY 9 cut(s) 46, 59, 114, 163, 171, 205, 225, 261, 292
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 1 cut(s) 317
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
EaeI YGGCCR 2 cut(s) 57, 259
EagI CGGCCG 2 cut(s) 57, 259
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
EciI GGCGGA 1 cut(s) 66
EclXI CGGCCG 2 cut(s) 57, 259
Eco24I GRGCYC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 183
Eco52I CGGCCG 2 cut(s) 57, 259
Eco88I CYCGRG 3 cut(s) 164, 220, 297
EcoO109I RGGNCCY 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 48
FaeI CATG 2 cut(s) 137, 156
FaiI YATR 2 cut(s) 135, 154
FaqI GGGAC 3 cut(s) 169, 175, 279
FatI CATG 2 cut(s) 133, 152
FauI CCCGC 1 cut(s) 236
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 6 cut(s) 17, 26, 57, 226, 235, 251
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 6 cut(s) 17, 26, 57, 226, 235, 251
GlaI GCGC 1 cut(s) 15
GluI GCNGC 6 cut(s) 17, 26, 57, 226, 235, 251
GsaI CCCAGC 1 cut(s) 175
HaeIII GGCC 3 cut(s) 59, 114, 261
HapII CCGG 4 cut(s) 48, 258, 262, 298
HhaI GCGC 1 cut(s) 16
Hin1II CATG 2 cut(s) 137, 156
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 1 cut(s) 314
HindII GTYRAC 1 cut(s) 314
HinfI GANTC 2 cut(s) 315, 340
HpaII CCGG 4 cut(s) 48, 258, 262, 298
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 3 cut(s) 139, 214, 314
Hpy188I TCNGA 2 cut(s) 320, 345
Hpy188III TCNNGA 1 cut(s) 192
Hpy8I GTNNAC 3 cut(s) 139, 214, 314
Hpy99I CGWCG 2 cut(s) 65, 204
HpyAV CCTTC 2 cut(s) 104, 294
HpyCH4III ACNGT 2 cut(s) 311, 349
HpyCH4V TGCA 3 cut(s) 28, 68, 150
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 25, 65, 225, 231, 234, 240
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 2 cut(s) 137, 156
HspAI GCGC 1 cut(s) 14
KflI GGGWCCC 1 cut(s) 183
KroI GCCGGC 1 cut(s) 257
KroNI GCCGGC 1 cut(s) 259
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 2 cut(s) 53, 286
Lsp1109I GCAGC 2 cut(s) 12, 212
MalI GATC 1 cut(s) 72
MbiI CCGCTC 2 cut(s) 56, 243
MboI GATC 1 cut(s) 70
MboII GAAGA 1 cut(s) 129
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 2 cut(s) 48, 178
MlyI GAGTC 1 cut(s) 309
MnlI CCTC 3 cut(s) 3, 113, 160
MroNI GCCGGC 1 cut(s) 257
MseI TTAA 1 cut(s) 352
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 4 cut(s) 48, 258, 262, 298
MspR9I CCNGG 5 cut(s) 35, 48, 263, 298, 299
MvaI CCWGG 1 cut(s) 35
MvnI CGCG 3 cut(s) 16, 234, 253
MwoI GCNNNNNNNGC 7 cut(s) 22, 25, 65, 225, 231, 234, 240
NaeI GCCGGC 1 cut(s) 259
NciI CCSGG 4 cut(s) 48, 263, 298, 299
NdeII GATC 1 cut(s) 70
NgoMIV GCCGGC 1 cut(s) 257
NlaIII CATG 2 cut(s) 137, 156
NlaIV GGNNCC 3 cut(s) 131, 184, 185
PaeR7I CTCGAG 2 cut(s) 164, 220
PdiI GCCGGC 1 cut(s) 259
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 6 cut(s) 18, 27, 58, 227, 236, 252
PleI GAGTC 1 cut(s) 309
PpsI GAGTC 1 cut(s) 309
PpuMI RGGWCCY 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 33
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 3 cut(s) 131, 184, 185
PspPI GGNCC 1 cut(s) 183
PspPPI RGGWCCY 1 cut(s) 183
PspXI VCTCGAGB 2 cut(s) 164, 220
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
SacII CCGCGG 1 cut(s) 235
SalI GTCGAC 1 cut(s) 312
SaqAI TTAA 1 cut(s) 352
SatI GCNGC 6 cut(s) 17, 26, 57, 226, 235, 251
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 5 cut(s) 35, 48, 263, 298, 299
SduI GDGCHC 2 cut(s) 48, 178
SetI ASST 3 cut(s) 36, 131, 227
Sfr274I CTCGAG 2 cut(s) 164, 220
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SinI GGWCC 1 cut(s) 183
SlaI CTCGAG 2 cut(s) 164, 220
SmaI CCCGGG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 164, 220
SmoI CTYRAG 2 cut(s) 164, 220
SsiI CCGC 8 cut(s) 16, 19, 51, 56, 232, 234, 243, 251
SspI AATATT 1 cut(s) 92
StyD4I CCNGG 5 cut(s) 33, 46, 261, 296, 297
TaaI ACNGT 2 cut(s) 311, 349
TaqI TCGA 3 cut(s) 165, 221, 313
TauI GCSGC 4 cut(s) 19, 59, 237, 253
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 1 cut(s) 352
Tru9I TTAA 1 cut(s) 352
TseI GCWGC 2 cut(s) 25, 225
TspGWI ACGGA 2 cut(s) 283, 344
TspMI CCCGGG 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 183
XhoI CTCGAG 2 cut(s) 164, 220
XmaI CCCGGG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.