Rroxscaffold_20G00445070

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000020
OG0000104
Physical Location & Seq
Forward (+)
25274 .. 25683
410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_20G00445070.1

Sequence Viewer

Length: 345 bp
ATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTGCGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGAACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGACGGACTGGGAACGCTCCTTCGGGGGCTTTCCGGGCGTTCAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTTGCTCGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.4

Weight (kDa)

11.3

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 1 cut(s) 214
AccII CGCG 3 cut(s) 135, 154, 286
AciI CCGC 6 cut(s) 133, 135, 144, 152, 286, 297
AclWI GGATC 2 cut(s) 31, 44
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 1 cut(s) 272
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 2 cut(s) 158, 286
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 2 cut(s) 208, 277
AluBI AGCT 1 cut(s) 126
AluI AGCT 1 cut(s) 126
AlwI GGATC 2 cut(s) 31, 44
Ama87I CYCGRG 2 cut(s) 47, 121
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 2 cut(s) 63, 126
ApoI RAATTY 1 cut(s) 272
ArsI GACNNNNNNTTYG 1 cut(s) 321
Asp700I GAANNNNTTC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 1 cut(s) 84
AsuC2I CCSGG 2 cut(s) 164, 200
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 2 cut(s) 47, 121
AvaII GGWCC 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 79
BamHI GGATCC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 50, 113
BceAI ACGGC 1 cut(s) 310
BcnI CCSGG 2 cut(s) 164, 200
BfrI CTTAAG 1 cut(s) 317
BisI GCNGC 4 cut(s) 64, 127, 136, 152
BlsI GCNGC 4 cut(s) 65, 128, 137, 153
Bme1390I CCNGG 2 cut(s) 164, 200
Bme18I GGWCC 1 cut(s) 84
BmeT110I CYCGRG 2 cut(s) 47, 121
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 3 cut(s) 38, 85, 86
BmrFI CCNGG 2 cut(s) 164, 200
BmrI ACTGGG 2 cut(s) 82, 183
BmuI ACTGGG 2 cut(s) 82, 183
BpuMI CCSGG 2 cut(s) 164, 200
BsaJI CCNNGG 3 cut(s) 46, 133, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 286
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 3 cut(s) 88, 178, 229
BseDI CCNNGG 3 cut(s) 46, 133, 163
BseLI CCNNNNNNNGG 2 cut(s) 158, 286
BseMII CTCAG 1 cut(s) 232
BseNI ACTGG 3 cut(s) 88, 178, 229
BseSI GKGCMC 1 cut(s) 79
BseX3I CGGCCG 1 cut(s) 160
BseXI GCAGC 2 cut(s) 50, 113
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 3 cut(s) 135, 154, 286
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 163
BsiHKCI CYCGRG 2 cut(s) 47, 121
BsiSI CCGG 3 cut(s) 159, 163, 199
BslFI GGGAC 3 cut(s) 70, 76, 180
BslI CCNNNNNNNGG 2 cut(s) 158, 286
BsmFI GGGAC 3 cut(s) 70, 76, 180
BsnI GGCC 1 cut(s) 162
BsoBI CYCGRG 2 cut(s) 47, 121
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 6 cut(s) 133, 135, 144, 152, 286, 297
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 3 cut(s) 135, 154, 286
BspLI GGNNCC 3 cut(s) 38, 85, 86
BspPI GGATC 2 cut(s) 31, 44
BspTI CTTAAG 1 cut(s) 317
BsrBI CCGCTC 1 cut(s) 144
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 3 cut(s) 88, 178, 229
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 3 cut(s) 46, 133, 163
BssMI GATC 1 cut(s) 36
Bst4CI ACNGT 1 cut(s) 212
BstAFI CTTAAG 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 156, 160
BstDEI CTNAG 1 cut(s) 218
BstDSI CCRYGG 1 cut(s) 133
BstFNI CGCG 3 cut(s) 135, 154, 286
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 1 cut(s) 39
BstMBI GATC 1 cut(s) 36
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 6 cut(s) 69, 126, 132, 135, 141, 200
BstSCI CCNGG 2 cut(s) 162, 198
BstSLI GKGCMC 1 cut(s) 79
BstUI CGCG 3 cut(s) 135, 154, 286
BstV1I GCAGC 2 cut(s) 50, 113
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BstZI CGGCCG 1 cut(s) 160
BsuRI GGCC 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 156, 160
CfoI GCGC 1 cut(s) 286
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 1 cut(s) 84
Cfr42I CCGCGG 1 cut(s) 136
Csp6I GTAC 1 cut(s) 228
CviJI RGCY 7 cut(s) 63, 72, 106, 126, 162, 194, 326
CviKI_1 RGCY 7 cut(s) 63, 72, 106, 126, 162, 194, 326
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 1 cut(s) 218
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 1 cut(s) 160
EagI CGGCCG 1 cut(s) 160
EclXI CGGCCG 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 84
Eco52I CGGCCG 1 cut(s) 160
Eco88I CYCGRG 2 cut(s) 47, 121
EcoO109I RGGNCCY 1 cut(s) 84
FaiI YATR 1 cut(s) 309
FaqI GGGAC 3 cut(s) 70, 76, 180
FauI CCCGC 3 cut(s) 137, 279, 290
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 4 cut(s) 64, 127, 136, 152
Fsp4HI GCNGC 4 cut(s) 64, 127, 136, 152
GlaI GCGC 1 cut(s) 285
GluI GCNGC 4 cut(s) 64, 127, 136, 152
GsaI CCCAGC 1 cut(s) 76
HaeIII GGCC 1 cut(s) 162
HapII CCGG 3 cut(s) 159, 163, 199
HhaI GCGC 1 cut(s) 286
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 215
HindII GTYRAC 1 cut(s) 215
HinfI GANTC 3 cut(s) 216, 241, 311
HpaII CCGG 3 cut(s) 159, 163, 199
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 3 cut(s) 115, 215, 292
Hpy188I TCNGA 2 cut(s) 221, 246
Hpy188III TCNNGA 2 cut(s) 49, 93
Hpy8I GTNNAC 3 cut(s) 115, 215, 292
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 1 cut(s) 195
HpyCH4III ACNGT 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 126, 132, 135, 141, 200
HpyF3I CTNAG 1 cut(s) 218
HspAI GCGC 1 cut(s) 284
KflI GGGWCCC 1 cut(s) 84
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
KspI CCGCGG 1 cut(s) 136
Kzo9I GATC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 7 cut(s) 58, 101, 159, 172, 176, 210, 212
Lsp1109I GCAGC 2 cut(s) 50, 113
MaeIII GTNAC 2 cut(s) 41, 302
MalI GATC 1 cut(s) 38
MbiI CCGCTC 1 cut(s) 144
MboI GATC 1 cut(s) 36
MboII GAAGA 1 cut(s) 280
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 79
MluCI AATT 1 cut(s) 272
MlyI GAGTC 2 cut(s) 210, 305
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 3 cut(s) 56, 61, 344
MroNI GCCGGC 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 1 cut(s) 318
MspA1I CMGCKG 1 cut(s) 135
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 3 cut(s) 159, 163, 199
MspR9I CCNGG 2 cut(s) 164, 200
MvnI CGCG 3 cut(s) 135, 154, 286
MwoI GCNNNNNNNGC 6 cut(s) 69, 126, 132, 135, 141, 200
NaeI GCCGGC 1 cut(s) 160
NciI CCSGG 2 cut(s) 164, 200
NdeII GATC 1 cut(s) 36
NgoMIV GCCGGC 1 cut(s) 158
NlaIV GGNNCC 3 cut(s) 38, 85, 86
PaeR7I CTCGAG 1 cut(s) 121
PdiI GCCGGC 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 4 cut(s) 65, 128, 137, 153
PleI GAGTC 2 cut(s) 210, 305
PpsI GAGTC 2 cut(s) 210, 305
PpuMI RGGWCCY 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 72
PspN4I GGNNCC 3 cut(s) 38, 85, 86
PspPI GGNCC 1 cut(s) 84
PspPPI RGGWCCY 1 cut(s) 84
PspXI VCTCGAGB 1 cut(s) 121
PsuI RGATCY 1 cut(s) 36
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
SacII CCGCGG 1 cut(s) 136
SalI GTCGAC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 4 cut(s) 64, 127, 136, 152
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 84
SchI GAGTC 2 cut(s) 210, 305
ScrFI CCNGG 2 cut(s) 164, 200
SduI GDGCHC 1 cut(s) 79
SetI ASST 3 cut(s) 48, 128, 324
Sfr274I CTCGAG 1 cut(s) 121
Sfr303I CCGCGG 1 cut(s) 136
SgrBI CCGCGG 1 cut(s) 136
SinI GGWCC 1 cut(s) 84
SlaI CTCGAG 1 cut(s) 121
SmlI CTYRAG 2 cut(s) 121, 317
SmoI CTYRAG 2 cut(s) 121, 317
Sse9I AATT 1 cut(s) 272
SsiI CCGC 6 cut(s) 133, 135, 144, 152, 286, 297
StyD4I CCNGG 2 cut(s) 162, 198
TaaI ACNGT 1 cut(s) 212
TaqI TCGA 3 cut(s) 122, 214, 254
TasI AATT 1 cut(s) 272
TauI GCSGC 2 cut(s) 138, 154
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseI GCWGC 2 cut(s) 63, 126
TspGWI ACGGA 3 cut(s) 29, 184, 245
Vha464I CTTAAG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 1 cut(s) 272
XhoI CTCGAG 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.