Rroxscaffold_27G00446650

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000027
OG0000104
Physical Location & Seq
Reverse (-)
78028 .. 79002
975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_27G00446650.1

Sequence Viewer

Length: 357 bp
ATGAACCGGAAGCCGGGTTACGGTGCCCAACTACGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGAACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGGACGGACTGGGAACGCTCCTTCGGGGGCTTTCCCCGGCGTTCAACGGTCGACTCGAACTGGTACGGACAAGGGGAATCCGACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

12.19

Weight (kDa)

11.83

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AccBSI CCGCTC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 317
AccII CGCG 3 cut(s) 36, 235, 254
AciI CCGC 5 cut(s) 107, 233, 235, 244, 252
AclWI GGATC 2 cut(s) 132, 145
AcoI YGGCCR 1 cut(s) 260
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 5 cut(s) 13, 20, 56, 258, 265
AgsI TTSAA 1 cut(s) 311
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
AlwI GGATC 2 cut(s) 132, 145
Ama87I CYCGRG 3 cut(s) 148, 165, 221
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 184
AsuC2I CCSGG 3 cut(s) 15, 264, 303
AsuHPI GGTGA 1 cut(s) 224
AvaI CYCGRG 3 cut(s) 148, 165, 221
AvaII GGWCC 1 cut(s) 184
BaeGI GKGCMC 2 cut(s) 28, 179
BamHI GGATCC 1 cut(s) 137
BanI GGYRCC 1 cut(s) 23
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 213
BcnI CCSGG 3 cut(s) 15, 264, 303
BfaI CTAG 1 cut(s) 44
BisI GCNGC 3 cut(s) 227, 236, 252
BlsI GCNGC 3 cut(s) 228, 237, 253
Bme1390I CCNGG 3 cut(s) 15, 264, 303
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 3 cut(s) 148, 165, 221
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 4 cut(s) 25, 139, 185, 186
BmrFI CCNGG 3 cut(s) 15, 264, 303
BmrI ACTGGG 2 cut(s) 182, 285
BmuI ACTGGG 2 cut(s) 182, 285
BoxI GACNNNNGTC 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 110
BpuMI CCSGG 3 cut(s) 15, 264, 303
BsaJI CCNNGG 4 cut(s) 147, 233, 263, 301
BsaWI WCCGGW 1 cut(s) 6
Bsc4I CCNNNNNNNGG 5 cut(s) 13, 20, 56, 258, 265
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 3 cut(s) 188, 280, 331
BseDI CCNNGG 4 cut(s) 147, 233, 263, 301
BseLI CCNNNNNNNGG 5 cut(s) 13, 20, 56, 258, 265
BseNI ACTGG 3 cut(s) 188, 280, 331
BseSI GKGCMC 2 cut(s) 28, 179
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 1 cut(s) 213
BseYI CCCAGC 1 cut(s) 172
Bsh1236I CGCG 3 cut(s) 36, 235, 254
Bsh1285I CGRYCG 2 cut(s) 263, 317
BshFI GGCC 1 cut(s) 262
BshNI GGYRCC 1 cut(s) 23
BsiEI CGRYCG 2 cut(s) 263, 317
BsiHKCI CYCGRG 3 cut(s) 148, 165, 221
BsiSI CCGG 5 cut(s) 7, 14, 259, 263, 303
BslFI GGGAC 3 cut(s) 170, 176, 282
BslI CCNNNNNNNGG 5 cut(s) 13, 20, 56, 258, 265
BsmFI GGGAC 3 cut(s) 170, 176, 282
BsnI GGCC 1 cut(s) 262
BsoBI CYCGRG 3 cut(s) 148, 165, 221
Bsp1286I GDGCHC 2 cut(s) 28, 179
Bsp143I GATC 1 cut(s) 137
BspACI CCGC 5 cut(s) 107, 233, 235, 244, 252
BspANI GGCC 1 cut(s) 262
BspFNI CGCG 3 cut(s) 36, 235, 254
BspLI GGNNCC 4 cut(s) 25, 139, 185, 186
BspPI GGATC 2 cut(s) 132, 145
BspT107I GGYRCC 1 cut(s) 23
BsrBI CCGCTC 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 3 cut(s) 188, 280, 331
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 4 cut(s) 147, 233, 263, 301
BssMI GATC 1 cut(s) 137
Bst4CI ACNGT 4 cut(s) 23, 86, 315, 352
BstC8I GCNNGC 2 cut(s) 256, 260
BstDEI CTNAG 1 cut(s) 110
BstDSI CCRYGG 1 cut(s) 233
BstFNI CGCG 3 cut(s) 36, 235, 254
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 1 cut(s) 140
BstMBI GATC 1 cut(s) 137
BstMCI CGRYCG 2 cut(s) 263, 317
BstMWI GCNNNNNNNGC 4 cut(s) 226, 232, 235, 241
BstPAI GACNNNNGTC 1 cut(s) 87
BstSCI CCNGG 3 cut(s) 13, 262, 301
BstSLI GKGCMC 2 cut(s) 28, 179
BstUI CGCG 3 cut(s) 36, 235, 254
BstV1I GCAGC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 137
BstYI RGATCY 1 cut(s) 137
BstZI CGGCCG 1 cut(s) 260
BsuRI GGCC 1 cut(s) 262
BtgI CCRYGG 1 cut(s) 233
Cac8I GCNNGC 2 cut(s) 256, 260
CfoI GCGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 1 cut(s) 184
Cfr42I CCGCGG 1 cut(s) 236
Csp6I GTAC 1 cut(s) 330
CviAII CATG 1 cut(s) 92
CviJI RGCY 7 cut(s) 13, 164, 172, 206, 226, 262, 296
CviKI_1 RGCY 7 cut(s) 13, 164, 172, 206, 226, 262, 296
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 1 cut(s) 110
DpnI GATC 1 cut(s) 139
DpnII GATC 1 cut(s) 137
EaeI YGGCCR 1 cut(s) 260
EagI CGGCCG 1 cut(s) 260
EclXI CGGCCG 1 cut(s) 260
Eco47I GGWCC 1 cut(s) 184
Eco52I CGGCCG 1 cut(s) 260
Eco88I CYCGRG 3 cut(s) 148, 165, 221
EcoO109I RGGNCCY 1 cut(s) 184
FaeI CATG 1 cut(s) 95
FaiI YATR 1 cut(s) 93
FaqI GGGAC 3 cut(s) 170, 176, 282
FatI CATG 1 cut(s) 91
FauI CCCGC 1 cut(s) 237
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 3 cut(s) 227, 236, 252
Fsp4HI GCNGC 3 cut(s) 227, 236, 252
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 37
GluI GCNGC 3 cut(s) 227, 236, 252
GsaI CCCAGC 1 cut(s) 176
HaeIII GGCC 1 cut(s) 262
HapII CCGG 5 cut(s) 7, 14, 259, 263, 303
HhaI GCGC 1 cut(s) 38
Hin1II CATG 1 cut(s) 95
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HinfI GANTC 2 cut(s) 319, 343
HpaII CCGG 5 cut(s) 7, 14, 259, 263, 303
HphI GGTGA 1 cut(s) 224
Hpy166II GTNNAC 2 cut(s) 215, 318
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 2 cut(s) 150, 193
Hpy8I GTNNAC 2 cut(s) 215, 318
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 297
HpyCH4III ACNGT 4 cut(s) 23, 86, 315, 352
HpyF10VI GCNNNNNNNGC 4 cut(s) 226, 232, 235, 241
HpyF3I CTNAG 1 cut(s) 110
Hsp92II CATG 1 cut(s) 95
HspAI GCGC 1 cut(s) 36
KflI GGGWCCC 1 cut(s) 184
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
KspI CCGCGG 1 cut(s) 236
Kzo9I GATC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 114, 289
Lsp1109I GCAGC 1 cut(s) 213
MaeI CTAG 1 cut(s) 44
MaeIII GTNAC 2 cut(s) 17, 142
MalI GATC 1 cut(s) 139
MbiI CCGCTC 1 cut(s) 244
MboI GATC 1 cut(s) 137
MflI RGATCY 1 cut(s) 137
MhlI GDGCHC 2 cut(s) 28, 179
MlyI GAGTC 1 cut(s) 313
MnlI CCTC 2 cut(s) 157, 161
MroNI GCCGGC 1 cut(s) 258
MseI TTAA 2 cut(s) 71, 355
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 5 cut(s) 7, 14, 259, 263, 303
MspR9I CCNGG 3 cut(s) 15, 264, 303
MvnI CGCG 3 cut(s) 36, 235, 254
MwoI GCNNNNNNNGC 4 cut(s) 226, 232, 235, 241
NaeI GCCGGC 1 cut(s) 260
NciI CCSGG 3 cut(s) 15, 264, 303
NdeII GATC 1 cut(s) 137
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 1 cut(s) 95
NlaIV GGNNCC 4 cut(s) 25, 139, 185, 186
PaeR7I CTCGAG 2 cut(s) 165, 221
PcsI WCGNNNNNNNCGW 1 cut(s) 319
PdiI GCCGGC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 343
PkrI GCNGC 3 cut(s) 228, 237, 253
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 184
PshAI GACNNNNGTC 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 184
PspFI CCCAGC 1 cut(s) 172
PspN4I GGNNCC 4 cut(s) 25, 139, 185, 186
PspPI GGNCC 1 cut(s) 184
PspPPI RGGWCCY 1 cut(s) 184
PspXI VCTCGAGB 2 cut(s) 165, 221
PsuI RGATCY 1 cut(s) 137
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
SacII CCGCGG 1 cut(s) 236
SalI GTCGAC 1 cut(s) 316
SaqAI TTAA 2 cut(s) 71, 355
SatI GCNGC 3 cut(s) 227, 236, 252
Sau3AI GATC 1 cut(s) 137
Sau96I GGNCC 1 cut(s) 184
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 3 cut(s) 15, 264, 303
SduI GDGCHC 2 cut(s) 28, 179
SetI ASST 3 cut(s) 45, 149, 228
Sfr274I CTCGAG 2 cut(s) 165, 221
Sfr303I CCGCGG 1 cut(s) 236
SgrBI CCGCGG 1 cut(s) 236
SinI GGWCC 1 cut(s) 184
SlaI CTCGAG 2 cut(s) 165, 221
SmlI CTYRAG 2 cut(s) 165, 221
SmoI CTYRAG 2 cut(s) 165, 221
SsiI CCGC 5 cut(s) 107, 233, 235, 244, 252
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 3 cut(s) 13, 262, 301
TaaI ACNGT 4 cut(s) 23, 86, 315, 352
TaqI TCGA 6 cut(s) 67, 100, 166, 222, 317, 322
TauI GCSGC 2 cut(s) 238, 254
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 2 cut(s) 71, 355
Tru9I TTAA 2 cut(s) 71, 355
TseI GCWGC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 130, 286, 347
VpaK11BI GGWCC 1 cut(s) 184
XhoI CTCGAG 2 cut(s) 165, 221
XmiI GTMKAC 1 cut(s) 317
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.