Rroxscaffold_20G00445030

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000020
OG0000104
Physical Location & Seq
Forward (+)
6417 .. 6823
407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_20G00445030.1

Sequence Viewer

Length: 354 bp
ATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTGCGAGGGCTGGGCACGGGGGTCCCGATCCCGAACCCGTCGGCTGTCGGTGAACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACGGGAACGCTCCTTCGGGGGCTTTCCCGGGCGTTCAACGCATTGCGATGGTCCTGCGGATGTTAACGCAATGTGATTTACGCCGTGCTGCGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

12.8

Weight (kDa)

11.27

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 144
AccII CGCG 3 cut(s) 135, 154, 295
AciI CCGC 7 cut(s) 133, 135, 144, 152, 229, 295, 306
AclWI GGATC 3 cut(s) 31, 44, 84
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 1 cut(s) 281
AfiI CCNNNNNNNGG 4 cut(s) 158, 165, 169, 295
AflII CTTAAG 1 cut(s) 326
AgsI TTSAA 2 cut(s) 209, 286
AluBI AGCT 1 cut(s) 126
AluI AGCT 1 cut(s) 126
AlwI GGATC 3 cut(s) 31, 44, 84
Ama87I CYCGRG 3 cut(s) 47, 121, 199
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 3 cut(s) 63, 126, 260
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 1 cut(s) 330
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 2 cut(s) 84, 223
AsuC2I CCSGG 3 cut(s) 164, 200, 201
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 3 cut(s) 47, 121, 199
AvaII GGWCC 2 cut(s) 84, 223
BaeGI GKGCMC 1 cut(s) 79
BamHI GGATCC 1 cut(s) 36
BbvI GCAGC 3 cut(s) 50, 113, 247
BccI CCATC 1 cut(s) 214
BceAI ACGGC 2 cut(s) 240, 319
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BcnI CCSGG 3 cut(s) 164, 200, 201
BfrI CTTAAG 1 cut(s) 326
BisI GCNGC 5 cut(s) 64, 127, 136, 152, 261
BlsI GCNGC 5 cut(s) 65, 128, 137, 153, 262
Bme1390I CCNGG 3 cut(s) 164, 200, 201
Bme18I GGWCC 2 cut(s) 84, 223
BmeT110I CYCGRG 3 cut(s) 47, 121, 199
BmgT120I GGNCC 2 cut(s) 84, 223
BmiI GGNNCC 3 cut(s) 38, 85, 86
BmrFI CCNGG 3 cut(s) 164, 200, 201
BpuMI CCSGG 3 cut(s) 164, 200, 201
BsaJI CCNNGG 4 cut(s) 46, 133, 163, 199
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 165, 169, 295
Bse118I RCCGGY 1 cut(s) 158
Bse3DI GCAATG 2 cut(s) 213, 248
BseDI CCNNGG 4 cut(s) 46, 133, 163, 199
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 4 cut(s) 158, 165, 169, 295
BseMI GCAATG 2 cut(s) 213, 248
BseSI GKGCMC 1 cut(s) 79
BseX3I CGGCCG 1 cut(s) 160
BseXI GCAGC 3 cut(s) 50, 113, 247
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 3 cut(s) 135, 154, 295
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 163
BsiHKCI CYCGRG 3 cut(s) 47, 121, 199
BsiSI CCGG 3 cut(s) 159, 163, 200
BslFI GGGAC 2 cut(s) 70, 181
BslI CCNNNNNNNGG 4 cut(s) 158, 165, 169, 295
BsmFI GGGAC 2 cut(s) 70, 181
BsnI GGCC 1 cut(s) 162
BsoBI CYCGRG 3 cut(s) 47, 121, 199
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 2 cut(s) 36, 89
BspACI CCGC 7 cut(s) 133, 135, 144, 152, 229, 295, 306
BspANI GGCC 1 cut(s) 162
BspFNI CGCG 3 cut(s) 135, 154, 295
BspLI GGNNCC 3 cut(s) 38, 85, 86
BspPI GGATC 3 cut(s) 31, 44, 84
BspTI CTTAAG 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 144
BsrDI GCAATG 2 cut(s) 213, 248
BsrFI RCCGGY 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 4 cut(s) 46, 133, 163, 199
BssMI GATC 2 cut(s) 36, 89
BstAFI CTTAAG 1 cut(s) 326
BstC8I GCNNGC 2 cut(s) 156, 160
BstDSI CCRYGG 1 cut(s) 133
BstF5I GGATG 1 cut(s) 237
BstFNI CGCG 3 cut(s) 135, 154, 295
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 2 cut(s) 39, 92
BstMBI GATC 2 cut(s) 36, 89
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 6 cut(s) 69, 126, 132, 135, 141, 210
BstSCI CCNGG 3 cut(s) 162, 198, 199
BstSLI GKGCMC 1 cut(s) 79
BstUI CGCG 3 cut(s) 135, 154, 295
BstV1I GCAGC 3 cut(s) 50, 113, 247
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BstZI CGGCCG 1 cut(s) 160
BsuRI GGCC 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 133
BtgZI GCGATG 1 cut(s) 233
BtsCI GGATG 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 156, 160
CfoI GCGC 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 84, 223
Cfr42I CCGCGG 1 cut(s) 136
Cfr9I CCCGGG 1 cut(s) 199
CviJI RGCY 7 cut(s) 63, 72, 106, 126, 162, 194, 335
CviKI_1 RGCY 7 cut(s) 63, 72, 106, 126, 162, 194, 335
DpnI GATC 2 cut(s) 38, 91
DpnII GATC 2 cut(s) 36, 89
EaeI YGGCCR 1 cut(s) 160
EagI CGGCCG 1 cut(s) 160
EclXI CGGCCG 1 cut(s) 160
Eco47I GGWCC 2 cut(s) 84, 223
Eco52I CGGCCG 1 cut(s) 160
Eco88I CYCGRG 3 cut(s) 47, 121, 199
EcoO109I RGGNCCY 1 cut(s) 84
FaiI YATR 1 cut(s) 318
FaqI GGGAC 2 cut(s) 70, 181
FauI CCCGC 3 cut(s) 137, 288, 299
Fnu4HI GCNGC 5 cut(s) 64, 127, 136, 152, 261
FokI GGATG 1 cut(s) 244
Fsp4HI GCNGC 5 cut(s) 64, 127, 136, 152, 261
GlaI GCGC 1 cut(s) 294
GluI GCNGC 5 cut(s) 64, 127, 136, 152, 261
GsaI CCCAGC 1 cut(s) 76
HaeIII GGCC 1 cut(s) 162
HapII CCGG 3 cut(s) 159, 163, 200
HhaI GCGC 1 cut(s) 295
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HinfI GANTC 1 cut(s) 320
HpaI GTTAAC 1 cut(s) 237
HpaII CCGG 3 cut(s) 159, 163, 200
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 3 cut(s) 115, 237, 301
Hpy188III TCNNGA 3 cut(s) 49, 87, 93
Hpy8I GTNNAC 3 cut(s) 115, 237, 301
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 126, 132, 135, 141, 210
HspAI GCGC 1 cut(s) 293
KflI GGGWCCC 1 cut(s) 84
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
KspAI GTTAAC 1 cut(s) 237
KspI CCGCGG 1 cut(s) 136
Kzo9I GATC 2 cut(s) 36, 89
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 5 cut(s) 58, 172, 176, 213, 239
Lsp1109I GCAGC 3 cut(s) 50, 113, 247
MaeIII GTNAC 2 cut(s) 41, 311
MalI GATC 2 cut(s) 38, 91
MbiI CCGCTC 1 cut(s) 144
MboI GATC 2 cut(s) 36, 89
MboII GAAGA 1 cut(s) 289
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 79
MluCI AATT 1 cut(s) 281
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 3 cut(s) 56, 61, 353
MroNI GCCGGC 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 2 cut(s) 236, 327
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 135
MspCI CTTAAG 1 cut(s) 326
MspI CCGG 3 cut(s) 159, 163, 200
MspR9I CCNGG 3 cut(s) 164, 200, 201
MvnI CGCG 3 cut(s) 135, 154, 295
MwoI GCNNNNNNNGC 6 cut(s) 69, 126, 132, 135, 141, 210
NaeI GCCGGC 1 cut(s) 160
NciI CCSGG 3 cut(s) 164, 200, 201
NdeII GATC 2 cut(s) 36, 89
NgoMIV GCCGGC 1 cut(s) 158
NlaIV GGNNCC 3 cut(s) 38, 85, 86
PaeR7I CTCGAG 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PdiI GCCGGC 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 281
PkrI GCNGC 5 cut(s) 65, 128, 137, 153, 262
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PpuMI RGGWCCY 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 72
PspN4I GGNNCC 3 cut(s) 38, 85, 86
PspPI GGNCC 2 cut(s) 84, 223
PspPPI RGGWCCY 1 cut(s) 84
PspXI VCTCGAGB 1 cut(s) 121
PsuI RGATCY 1 cut(s) 36
RseI CAYNNNNRTG 1 cut(s) 218
SacII CCGCGG 1 cut(s) 136
SaqAI TTAA 2 cut(s) 236, 327
SatI GCNGC 5 cut(s) 64, 127, 136, 152, 261
Sau3AI GATC 2 cut(s) 36, 89
Sau96I GGNCC 2 cut(s) 84, 223
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 164, 200, 201
SduI GDGCHC 1 cut(s) 79
SetI ASST 3 cut(s) 48, 128, 333
Sfr274I CTCGAG 1 cut(s) 121
Sfr303I CCGCGG 1 cut(s) 136
SgrBI CCGCGG 1 cut(s) 136
SinI GGWCC 2 cut(s) 84, 223
SlaI CTCGAG 1 cut(s) 121
SmaI CCCGGG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 2 cut(s) 121, 326
SmoI CTYRAG 2 cut(s) 121, 326
Sse9I AATT 1 cut(s) 281
SsiI CCGC 7 cut(s) 133, 135, 144, 152, 229, 295, 306
StyD4I CCNGG 3 cut(s) 162, 198, 199
TaqI TCGA 1 cut(s) 122
TasI AATT 1 cut(s) 281
TauI GCSGC 2 cut(s) 138, 154
Tru1I TTAA 2 cut(s) 236, 327
Tru9I TTAA 2 cut(s) 236, 327
TseI GCWGC 3 cut(s) 63, 126, 260
TspGWI ACGGA 2 cut(s) 29, 185
TspMI CCCGGG 1 cut(s) 199
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 84, 223
XapI RAATTY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 121
XmaI CCCGGG 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.