pycom2575g00020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002575
OG0000104
Physical Location & Seq
Reverse (-)
10232 .. 12333
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGACTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGCCTATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTTTTTACCCTACTGATGACAGCGTCGCAATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTCAAAATGTCGACCGTGGCATGCTAGTGACATGCCATGCCTCGCAAAATGCCAATGACATGCCAAGCCTCGCAAAATGTCGACCGTGGCATGCCAATGACATGCCAAGCCAAGCCTCGCAAAATGTCGACCGTGGCATGCCAATGACATGCCAATGGTCGCAAAATGTCGGCCATGGCATGCCAATGACATGCCAAGCCTCGCAAAATGTCGACCGTGGCATGCCAATGACATGCCAATGTCATGCCAAGCCTCGCAAAATGTCGACCATGGCATGCCAAGGGTCGCAAAATGTCGACCATGGCATGCCAATGACATGCCAAGCCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.09

Weight (kDa)

9.04

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 6 cut(s) 279, 349, 396, 480, 533, 564
AccII CGCG 2 cut(s) 235, 259
AclWI GGATC 1 cut(s) 54
AcoI YGGCCR 1 cut(s) 439
AcsI RAATTY 1 cut(s) 77
AfaI GTAC 2 cut(s) 28, 199
AgsI TTSAA 3 cut(s) 83, 191, 245
AluBI AGCT 2 cut(s) 86, 264
AluI AGCT 2 cut(s) 86, 264
AlwI GGATC 1 cut(s) 54
AoxI GGCC 2 cut(s) 51, 439
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AspLEI GCGC 2 cut(s) 237, 259
BbvI GCAGC 1 cut(s) 142
BfaI CTAG 3 cut(s) 87, 195, 293
BisI GCNGC 1 cut(s) 131
BlsI GCNGC 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 208
Bsa29I ATCGAT 1 cut(s) 58
BsaJI CCNNGG 8 cut(s) 283, 353, 400, 442, 484, 537, 547, 568
Bse1I ACTGG 3 cut(s) 24, 124, 251
BseCI ATCGAT 1 cut(s) 58
BseDI CCNNGG 8 cut(s) 283, 353, 400, 442, 484, 537, 547, 568
BseNI ACTGG 3 cut(s) 24, 124, 251
BseXI GCAGC 1 cut(s) 142
Bsh1236I CGCG 2 cut(s) 235, 259
Bsh1285I CGRYCG 4 cut(s) 283, 353, 400, 484
BshFI GGCC 2 cut(s) 53, 441
BshVI ATCGAT 1 cut(s) 58
BsiEI CGRYCG 4 cut(s) 283, 353, 400, 484
BsnI GGCC 2 cut(s) 53, 441
Bsp143I GATC 1 cut(s) 59
Bsp19I CCATGG 3 cut(s) 442, 537, 568
BspANI GGCC 2 cut(s) 53, 441
BspDI ATCGAT 1 cut(s) 58
BspFNI CGCG 2 cut(s) 235, 259
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 1 cut(s) 54
BsrI ACTGG 3 cut(s) 24, 124, 251
BssECI CCNNGG 8 cut(s) 283, 353, 400, 442, 484, 537, 547, 568
BssMI GATC 1 cut(s) 59
BssT1I CCWWGG 4 cut(s) 442, 537, 547, 568
Bst4CI ACNGT 7 cut(s) 41, 211, 269, 284, 354, 401, 485
BstC8I GCNNGC 7 cut(s) 290, 360, 407, 449, 491, 544, 575
BstDSI CCRYGG 7 cut(s) 283, 353, 400, 442, 484, 537, 568
BstFNI CGCG 2 cut(s) 235, 259
BstHHI GCGC 2 cut(s) 237, 259
BstKTI GATC 1 cut(s) 62
BstMBI GATC 1 cut(s) 59
BstMCI CGRYCG 4 cut(s) 283, 353, 400, 484
BstMWI GCNNNNNNNGC 2 cut(s) 144, 256
BstUI CGCG 2 cut(s) 235, 259
BstV1I GCAGC 1 cut(s) 142
Bsu15I ATCGAT 1 cut(s) 58
BsuRI GGCC 2 cut(s) 53, 441
BsuTUI ATCGAT 1 cut(s) 58
BtgI CCRYGG 7 cut(s) 283, 353, 400, 442, 484, 537, 568
BtgZI GCGATG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 7 cut(s) 290, 360, 407, 449, 491, 544, 575
CfoI GCGC 2 cut(s) 237, 259
ClaI ATCGAT 1 cut(s) 58
CseI GACGC 1 cut(s) 163
Csp6I GTAC 2 cut(s) 27, 198
CviQI GTAC 2 cut(s) 27, 198
DpnI GATC 1 cut(s) 61
DpnII GATC 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 439
Eco130I CCWWGG 4 cut(s) 442, 537, 547, 568
EcoT14I CCWWGG 4 cut(s) 442, 537, 547, 568
ErhI CCWWGG 4 cut(s) 442, 537, 547, 568
FblI GTMKAC 6 cut(s) 279, 349, 396, 480, 533, 564
Fnu4HI GCNGC 1 cut(s) 131
Fsp4HI GCNGC 1 cut(s) 131
FspBI CTAG 3 cut(s) 87, 195, 293
GlaI GCGC 2 cut(s) 236, 258
GluI GCNGC 1 cut(s) 131
HaeIII GGCC 2 cut(s) 53, 441
HgaI GACGC 1 cut(s) 163
HhaI GCGC 2 cut(s) 237, 259
Hin6I GCGC 2 cut(s) 235, 257
HinP1I GCGC 2 cut(s) 235, 257
HincII GTYRAC 6 cut(s) 280, 350, 397, 481, 534, 565
HindII GTYRAC 6 cut(s) 280, 350, 397, 481, 534, 565
HinfI GANTC 3 cut(s) 4, 13, 215
Hpy166II GTNNAC 6 cut(s) 280, 350, 397, 481, 534, 565
Hpy188I TCNGA 3 cut(s) 18, 76, 98
Hpy8I GTNNAC 6 cut(s) 280, 350, 397, 481, 534, 565
Hpy99I CGWCG 2 cut(s) 152, 179
HpyAV CCTTC 1 cut(s) 81
HpyCH4III ACNGT 7 cut(s) 41, 211, 269, 284, 354, 401, 485
HpyCH4IV ACGT 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 256
HpySE526I ACGT 1 cut(s) 150
HspAI GCGC 2 cut(s) 235, 257
Kzo9I GATC 1 cut(s) 59
LpnPI CCDG 4 cut(s) 37, 96, 105, 264
Lsp1109I GCAGC 1 cut(s) 142
MaeI CTAG 3 cut(s) 87, 195, 293
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 2 cut(s) 103, 295
MalI GATC 1 cut(s) 61
MboI GATC 1 cut(s) 59
MfeI CAATTG 1 cut(s) 223
MluCI AATT 3 cut(s) 77, 186, 223
MnlI CCTC 7 cut(s) 83, 197, 319, 347, 394, 478, 531
MslI CAYNNNNRTG 8 cut(s) 293, 363, 410, 421, 452, 494, 505, 578
MunI CAATTG 1 cut(s) 223
MvnI CGCG 2 cut(s) 235, 259
MwoI GCNNNNNNNGC 2 cut(s) 144, 256
NcoI CCATGG 3 cut(s) 442, 537, 568
NdeII GATC 1 cut(s) 59
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 295
PaeI GCATGC 7 cut(s) 292, 362, 409, 451, 493, 546, 577
PfeI GAWTC 2 cut(s) 13, 215
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 2 cut(s) 28, 199
RsaNI GTAC 2 cut(s) 27, 198
RseI CAYNNNNRTG 8 cut(s) 293, 363, 410, 421, 452, 494, 505, 578
SalI GTCGAC 6 cut(s) 278, 348, 395, 479, 532, 563
SatI GCNGC 1 cut(s) 131
Sau3AI GATC 1 cut(s) 59
SetI ASST 6 cut(s) 73, 88, 94, 153, 196, 266
SmiMI CAYNNNNRTG 8 cut(s) 293, 363, 410, 421, 452, 494, 505, 578
SphI GCATGC 7 cut(s) 292, 362, 409, 451, 493, 546, 577
Sse9I AATT 3 cut(s) 77, 186, 223
SspMI CTAG 3 cut(s) 87, 195, 293
StyI CCWWGG 4 cut(s) 442, 537, 547, 568
TaaI ACNGT 7 cut(s) 41, 211, 269, 284, 354, 401, 485
TaiI ACGT 1 cut(s) 153
TaqI TCGA 7 cut(s) 58, 279, 349, 396, 480, 533, 564
TasI AATT 3 cut(s) 77, 186, 223
TfiI GAWTC 2 cut(s) 13, 215
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 131
TspRI CASTG 1 cut(s) 258
Tth111I GACNNNGTC 1 cut(s) 173
XapI RAATTY 1 cut(s) 77
XmiI GTMKAC 6 cut(s) 279, 349, 396, 480, 533, 564
XspI CTAG 3 cut(s) 87, 195, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.