pycom520g01350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000104
Physical Location & Seq
Reverse (-)
1120414 .. 1120712
299 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 299 bp
TACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGAATGGGCACGGGGGTCCCAGCCTGGAACCCTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTGCCGCGTGCCGGCCGGGGGAAAGACTGGGAACGGTCCCTTCGGGGGCCTTCCCCGGGCAGCGAACATTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

10.55

Weight (kDa)

11.08

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000104)

Species Orthologous Gene IDs
pyrus_communis pycom2246g00050 pycom2246g00060 pycom2246g00070 pycom2246g00100 pycom2246g00170 pycom2575g00020 pycom520g01350
rosa_roxburghii Rroxscaffold_102G00450880 Rroxscaffold_102G00450940 Rroxscaffold_103G00450570 Rroxscaffold_104G00444660 Rroxscaffold_104G00444710 Rroxscaffold_104G00444740 Rroxscaffold_104G00444800 Rroxscaffold_104G00444860 Rroxscaffold_105G00447090 Rroxscaffold_105G00447190 Rroxscaffold_105G00447230 Rroxscaffold_106G00451430 Rroxscaffold_107G00442440 Rroxscaffold_107G00442550 Rroxscaffold_107G00442640 Rroxscaffold_107G00442690 Rroxscaffold_111G00451560 Rroxscaffold_112G00451580 Rroxscaffold_113G00451620 Rroxscaffold_115G00451690 Rroxscaffold_118G00451780 Rroxscaffold_122G00451870 Rroxscaffold_123G00451910 Rroxscaffold_124G00451960 Rroxscaffold_128G00452050 Rroxscaffold_12G00450640 Rroxscaffold_131G00452100 Rroxscaffold_132G00452130 Rroxscaffold_138G00452190 Rroxscaffold_13G00448670 Rroxscaffold_14G00441890 Rroxscaffold_14G00441960 Rroxscaffold_14G00442010 Rroxscaffold_14G00442070 Rroxscaffold_15G00447460 Rroxscaffold_15G00447510 Rroxscaffold_15G00447580 Rroxscaffold_162G00450750 Rroxscaffold_17G00435460 Rroxscaffold_17G00435510 Rroxscaffold_17G00435570 Rroxscaffold_17G00435640 Rroxscaffold_17G00435910 Rroxscaffold_18G00446090 Rroxscaffold_18G00446110 Rroxscaffold_18G00446170 Rroxscaffold_19G00448930 Rroxscaffold_1G00000010 Rroxscaffold_1G00000090 Rroxscaffold_1G00000130 Rroxscaffold_20G00445030 Rroxscaffold_20G00445040 Rroxscaffold_20G00445070 Rroxscaffold_20G00445110 Rroxscaffold_21G00439440 Rroxscaffold_21G00439460 Rroxscaffold_21G00439470 Rroxscaffold_21G00439520 Rroxscaffold_21G00439570 Rroxscaffold_21G00439660 Rroxscaffold_22G00439850 Rroxscaffold_22G00439990 Rroxscaffold_22G00440030 Rroxscaffold_25G00450140 Rroxscaffold_25G00450170 Rroxscaffold_26G00447770 Rroxscaffold_27G00446650 Rroxscaffold_29G00441730 Rroxscaffold_29G00441740 Rroxscaffold_29G00441760 Rroxscaffold_29G00441780 Rroxscaffold_29G00441830 Rroxscaffold_29G00441870 Rroxscaffold_30G00449490 Rroxscaffold_30G00449510 Rroxscaffold_31G00438130 Rroxscaffold_31G00438170 Rroxscaffold_31G00438270 Rroxscaffold_32G00442790 Rroxscaffold_32G00442800 Rroxscaffold_32G00442840 Rroxscaffold_33G00439700 Rroxscaffold_33G00439710 Rroxscaffold_34G00443170 Rroxscaffold_34G00443210 Rroxscaffold_34G00443280 Rroxscaffold_34G00443310 Rroxscaffold_35G00441010 Rroxscaffold_35G00441120 Rroxscaffold_35G00441180 Rroxscaffold_35G00441260 Rroxscaffold_35G00441290 Rroxscaffold_36G00440070 Rroxscaffold_36G00440110 Rroxscaffold_36G00440140 Rroxscaffold_36G00440190 Rroxscaffold_37G00445180 Rroxscaffold_37G00445190 Rroxscaffold_37G00445230 Rroxscaffold_37G00445240 Rroxscaffold_37G00445270 Rroxscaffold_38G00444450 Rroxscaffold_38G00444530 Rroxscaffold_38G00444570 Rroxscaffold_38G00444600 Rroxscaffold_38G00444620 Rroxscaffold_39G00448260 Rroxscaffold_40G00447720 Rroxscaffold_43G00449810 Rroxscaffold_43G00449840 Rroxscaffold_43G00449900 Rroxscaffold_44G00440460 Rroxscaffold_44G00440470 Rroxscaffold_44G00440510 Rroxscaffold_44G00440600 Rroxscaffold_44G00440640 Rroxscaffold_45G00438810 Rroxscaffold_45G00438840 Rroxscaffold_45G00438870 Rroxscaffold_45G00438890 Rroxscaffold_45G00438980 Rroxscaffold_46G00448720 Rroxscaffold_46G00448740 Rroxscaffold_47G00443950 Rroxscaffold_47G00443980 Rroxscaffold_47G00444000 Rroxscaffold_47G00444090 Rroxscaffold_48G00448430 Rroxscaffold_48G00448470 Rroxscaffold_48G00448510 Rroxscaffold_49G00438600 Rroxscaffold_49G00438620 Rroxscaffold_49G00438680 Rroxscaffold_49G00438710 Rroxscaffold_49G00438740 Rroxscaffold_49G00438780 Rroxscaffold_50G00444160 Rroxscaffold_50G00444260 Rroxscaffold_50G00444290 Rroxscaffold_50G00444370 Rroxscaffold_50G00444400 Rroxscaffold_53G00449700 Rroxscaffold_53G00449730 Rroxscaffold_53G00449740 Rroxscaffold_54G00443410 Rroxscaffold_54G00443460 Rroxscaffold_54G00443490 Rroxscaffold_54G00443530 Rroxscaffold_54G00443580 Rroxscaffold_54G00443610 Rroxscaffold_54G00443670 Rroxscaffold_55G00450070 Rroxscaffold_55G00450130 Rroxscaffold_56G00450950 Rroxscaffold_58G00449000 Rroxscaffold_58G00449050 Rroxscaffold_59G00442150 Rroxscaffold_59G00442180 Rroxscaffold_59G00442250 Rroxscaffold_59G00442270 Rroxscaffold_59G00442280 Rroxscaffold_60G00448270 Rroxscaffold_60G00448290 Rroxscaffold_60G00448330 Rroxscaffold_60G00448400 Rroxscaffold_61G00449970 Rroxscaffold_62G00437930 Rroxscaffold_62G00438030 Rroxscaffold_64G00450690 Rroxscaffold_64G00450700 Rroxscaffold_65G00445280 Rroxscaffold_65G00445350 Rroxscaffold_65G00445390 Rroxscaffold_66G00437570 Rroxscaffold_66G00437600 Rroxscaffold_66G00437620 Rroxscaffold_66G00437690 Rroxscaffold_66G00437730 Rroxscaffold_66G00437770 Rroxscaffold_66G00437860 Rroxscaffold_68G00447000 Rroxscaffold_68G00447040 Rroxscaffold_69G00446680 Rroxscaffold_69G00446780 Rroxscaffold_69G00446790 Rroxscaffold_69G00446810 Rroxscaffold_6G00387760 Rroxscaffold_6G00387790 Rroxscaffold_6G00387830 Rroxscaffold_6G00387840 Rroxscaffold_70G00446290 Rroxscaffold_70G00446390 Rroxscaffold_71G00445730 Rroxscaffold_71G00445750 Rroxscaffold_72G00449340 Rroxscaffold_72G00449380 Rroxscaffold_73G00439090 Rroxscaffold_73G00439120 Rroxscaffold_73G00439190 Rroxscaffold_73G00439210 Rroxscaffold_73G00439250 Rroxscaffold_74G00442890 Rroxscaffold_74G00442960 Rroxscaffold_75G00447280 Rroxscaffold_75G00447300 Rroxscaffold_75G00447340 Rroxscaffold_75G00447390 Rroxscaffold_75G00447410 Rroxscaffold_75G00447440 Rroxscaffold_76G00448590 Rroxscaffold_78G00449630 Rroxscaffold_78G00449640 Rroxscaffold_79G00450250 Rroxscaffold_80G00450280 Rroxscaffold_80G00450300 Rroxscaffold_80G00450310 Rroxscaffold_81G00450490 Rroxscaffold_82G00450810 Rroxscaffold_86G00451260 Rroxscaffold_87G00451290 Rroxscaffold_87G00451300 Rroxscaffold_87G00451320 Rroxscaffold_88G00451390 Rroxscaffold_90G00448810 Rroxscaffold_91G00442980 Rroxscaffold_91G00443040 Rroxscaffold_92G00446460 Rroxscaffold_92G00446490 Rroxscaffold_92G00446500 Rroxscaffold_92G00446520 Rroxscaffold_92G00446540 Rroxscaffold_92G00446560 Rroxscaffold_93G00440970 Rroxscaffold_93G00440990 Rroxscaffold_94G00445800 Rroxscaffold_94G00445870 Rroxscaffold_94G00445950 Rroxscaffold_94G00445990 Rroxscaffold_95G00449230 Rroxscaffold_95G00449260 Rroxscaffold_95G00449270 Rroxscaffold_96G00449080 Rroxscaffold_97G00442330 Rroxscaffold_97G00442350 Rroxscaffold_97G00442360 Rroxscaffold_98G00451340 Rroxscaffold_99G00451190 Rroxscaffold_99G00451210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccBSI CCGCTC 1 cut(s) 181
AccII CGCG 2 cut(s) 172, 190
AciI CCGC 6 cut(s) 4, 150, 170, 172, 181, 188
AclWI GGATC 2 cut(s) 69, 82
AcoI YGGCCR 1 cut(s) 196
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 4 cut(s) 194, 201, 228, 239
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 2 cut(s) 163, 257
AluI AGCT 2 cut(s) 163, 257
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 2 cut(s) 69, 82
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 2 cut(s) 158, 238
AoxI GGCC 2 cut(s) 196, 230
ApeKI GCWGC 2 cut(s) 163, 243
AspS9I GGNCC 3 cut(s) 122, 219, 230
AsuC2I CCSGG 3 cut(s) 200, 239, 240
AsuHPI GGTGA 1 cut(s) 33
AvaI CYCGRG 2 cut(s) 158, 238
AvaII GGWCC 2 cut(s) 122, 219
BaeGI GKGCMC 1 cut(s) 117
BamHI GGATCC 1 cut(s) 74
BanI GGYRCC 1 cut(s) 184
BbvI GCAGC 2 cut(s) 150, 255
BccI CCATC 1 cut(s) 33
BciT130I CCWGG 1 cut(s) 131
BcnI CCSGG 3 cut(s) 200, 239, 240
BcoDI GTCTC 1 cut(s) 18
BisI GCNGC 4 cut(s) 164, 173, 188, 244
BlsI GCNGC 4 cut(s) 165, 174, 189, 245
Bme1390I CCNGG 4 cut(s) 131, 200, 239, 240
Bme18I GGWCC 2 cut(s) 122, 219
BmeT110I CYCGRG 2 cut(s) 158, 238
BmgT120I GGNCC 3 cut(s) 122, 219, 230
BmiI GGNNCC 7 cut(s) 76, 123, 124, 135, 186, 221, 231
BmrFI CCNGG 4 cut(s) 131, 200, 239, 240
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 1 cut(s) 15
BmuI ACTGGG 1 cut(s) 220
BpuMI CCSGG 3 cut(s) 200, 239, 240
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 6 cut(s) 17, 138, 170, 199, 237, 238
Bsc4I CCNNNNNNNGG 4 cut(s) 194, 201, 228, 239
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 215, 269
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 6 cut(s) 17, 138, 170, 199, 237, 238
BseLI CCNNNNNNNGG 4 cut(s) 194, 201, 228, 239
BseMII CTCAG 2 cut(s) 95, 272
BseNI ACTGG 2 cut(s) 215, 269
BseSI GKGCMC 1 cut(s) 117
BseX3I CGGCCG 1 cut(s) 196
BseXI GCAGC 2 cut(s) 150, 255
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 2 cut(s) 172, 190
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 2 cut(s) 198, 232
BshNI GGYRCC 1 cut(s) 184
BsiEI CGRYCG 1 cut(s) 199
BsiHKCI CYCGRG 2 cut(s) 158, 238
BsiSI CCGG 3 cut(s) 195, 199, 239
BslFI GGGAC 2 cut(s) 108, 205
BslI CCNNNNNNNGG 4 cut(s) 194, 201, 228, 239
BsmAI GTCTC 1 cut(s) 18
BsmFI GGGAC 2 cut(s) 108, 205
BsnI GGCC 2 cut(s) 198, 232
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 2 cut(s) 158, 238
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 1 cut(s) 74
BspACI CCGC 6 cut(s) 4, 150, 170, 172, 181, 188
BspANI GGCC 2 cut(s) 198, 232
BspCNI CTCAG 2 cut(s) 96, 271
BspFNI CGCG 2 cut(s) 172, 190
BspLI GGNNCC 7 cut(s) 76, 123, 124, 135, 186, 221, 231
BspPI GGATC 2 cut(s) 69, 82
BspT107I GGYRCC 1 cut(s) 184
BspTNI GGTCTC 1 cut(s) 18
BsrBI CCGCTC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 2 cut(s) 215, 269
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 6 cut(s) 17, 138, 170, 199, 237, 238
BssMI GATC 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 17
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 219, 290
BstC8I GCNNGC 2 cut(s) 192, 196
BstDEI CTNAG 2 cut(s) 104, 258
BstDSI CCRYGG 1 cut(s) 170
BstFNI CGCG 2 cut(s) 172, 190
BstKTI GATC 1 cut(s) 77
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 1 cut(s) 74
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 5 cut(s) 163, 169, 172, 178, 187
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 4 cut(s) 129, 198, 237, 238
BstSLI GKGCMC 1 cut(s) 117
BstUI CGCG 2 cut(s) 172, 190
BstV1I GCAGC 2 cut(s) 150, 255
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
BstZI CGGCCG 1 cut(s) 196
BsuRI GGCC 2 cut(s) 198, 232
BtgI CCRYGG 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 192, 196
CaiI CAGNNNCTG 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 3 cut(s) 122, 219, 230
Cfr42I CCGCGG 1 cut(s) 173
Cfr9I CCCGGG 1 cut(s) 238
Csp6I GTAC 1 cut(s) 268
CviJI RGCY 8 cut(s) 29, 101, 129, 143, 163, 198, 232, 257
CviKI_1 RGCY 8 cut(s) 29, 101, 129, 143, 163, 198, 232, 257
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 2 cut(s) 104, 258
DpnI GATC 1 cut(s) 76
DpnII GATC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 196
EagI CGGCCG 1 cut(s) 196
EciI GGCGGA 1 cut(s) 165
EclXI CGGCCG 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 17
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 122, 219
Eco52I CGGCCG 1 cut(s) 196
Eco88I CYCGRG 2 cut(s) 158, 238
EcoO109I RGGNCCY 2 cut(s) 122, 230
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 17
ErhI CCWWGG 1 cut(s) 17
FaqI GGGAC 2 cut(s) 108, 205
FauI CCCGC 2 cut(s) 174, 177
Fnu4HI GCNGC 4 cut(s) 164, 173, 188, 244
Fsp4HI GCNGC 4 cut(s) 164, 173, 188, 244
GluI GCNGC 4 cut(s) 164, 173, 188, 244
GsaI CCCAGC 1 cut(s) 129
HaeIII GGCC 2 cut(s) 198, 232
HapII CCGG 3 cut(s) 195, 199, 239
HinfI GANTC 1 cut(s) 281
HpaII CCGG 3 cut(s) 195, 199, 239
HphI GGTGA 1 cut(s) 33
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 105, 261, 286
Hpy188III TCNNGA 1 cut(s) 87
Hpy8I GTNNAC 1 cut(s) 24
HpyAV CCTTC 2 cut(s) 233, 243
HpyCH4III ACNGT 2 cut(s) 219, 290
HpyF10VI GCNNNNNNNGC 5 cut(s) 163, 169, 172, 178, 187
HpyF3I CTNAG 2 cut(s) 104, 258
KflI GGGWCCC 1 cut(s) 122
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
KspI CCGCGG 1 cut(s) 173
Kzo9I GATC 1 cut(s) 74
LmnI GCTCC 1 cut(s) 171
LpnPI CCDG 9 cut(s) 18, 116, 139, 143, 196, 208, 212, 250, 252
Lsp1109I GCAGC 2 cut(s) 150, 255
LweI GCATC 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 79
MalI GATC 1 cut(s) 76
MbiI CCGCTC 1 cut(s) 181
MboI GATC 1 cut(s) 74
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 1 cut(s) 294
MnlI CCTC 3 cut(s) 40, 99, 148
MroNI GCCGGC 1 cut(s) 194
MseI TTAA 2 cut(s) 293, 297
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 3 cut(s) 195, 199, 239
MspR9I CCNGG 4 cut(s) 131, 200, 239, 240
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 2 cut(s) 172, 190
MwoI GCNNNNNNNGC 5 cut(s) 163, 169, 172, 178, 187
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 3 cut(s) 200, 239, 240
NdeII GATC 1 cut(s) 74
NgoMIV GCCGGC 1 cut(s) 194
NlaIV GGNNCC 7 cut(s) 76, 123, 124, 135, 186, 221, 231
NmeAIII GCCGAG 1 cut(s) 119
PacI TTAATTAA 1 cut(s) 297
PaeR7I CTCGAG 1 cut(s) 158
PdiI GCCGGC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 281
PkrI GCNGC 4 cut(s) 165, 174, 189, 245
PpuMI RGGWCCY 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 129
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 7 cut(s) 76, 123, 124, 135, 186, 221, 231
PspPI GGNCC 3 cut(s) 122, 219, 230
PspPPI RGGWCCY 1 cut(s) 122
PspXI VCTCGAGB 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 74
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacII CCGCGG 1 cut(s) 173
SaqAI TTAA 2 cut(s) 293, 297
SatI GCNGC 4 cut(s) 164, 173, 188, 244
Sau3AI GATC 1 cut(s) 74
Sau96I GGNCC 3 cut(s) 122, 219, 230
ScrFI CCNGG 4 cut(s) 131, 200, 239, 240
SduI GDGCHC 1 cut(s) 117
SetI ASST 4 cut(s) 14, 23, 165, 259
SfaNI GCATC 1 cut(s) 15
Sfr274I CTCGAG 1 cut(s) 158
Sfr303I CCGCGG 1 cut(s) 173
SgrBI CCGCGG 1 cut(s) 173
SinI GGWCC 2 cut(s) 122, 219
SlaI CTCGAG 1 cut(s) 158
SmaI CCCGGG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 1 cut(s) 294
SsiI CCGC 6 cut(s) 4, 150, 170, 172, 181, 188
StyD4I CCNGG 4 cut(s) 129, 198, 237, 238
StyI CCWWGG 1 cut(s) 17
TaaI ACNGT 2 cut(s) 219, 290
TaqI TCGA 2 cut(s) 37, 159
TasI AATT 1 cut(s) 294
TauI GCSGC 2 cut(s) 175, 190
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 2 cut(s) 293, 297
Tru9I TTAA 2 cut(s) 293, 297
TseI GCWGC 2 cut(s) 163, 243
TspGWI ACGGA 2 cut(s) 67, 285
TspMI CCCGGG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 122, 219
XhoI CTCGAG 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.