Rmu_sc0003778.1_g000006

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003778.1
OG0000178
Physical Location & Seq
Reverse (-)
19814 .. 21783
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003778.1_g000006.1.cds

Sequence Viewer

Length: 1263 bp
atggacactgaaggcaagacgaatgattcatataagactcgtcttgatatggaagagatgggaatcaagtctgacttgcaccccaaatctattgatagtgtaattaagtttatattagcatcttatactttcagtacagatgagagaaaatattttcgtgagttatgtaattgtacaaaacttccagatgagatgtcttcaaatatctttaattgcgtgaacattgatgattccaagatttatgggctgaaaagtcatgattgccacattatattgcagcggcttcttcctgttgccttgcgtgggtacttgtctaaaggtgttcgtgaggtcattatcaaactttgtttgttctttaaggagtggacattcaagactttgagattgaatattttggaaagactagataaagatattgtcgtaattctgtgcaagctagagttgaacttcccaccttcattttttaacattatgactcacttgccaattcacctagcatacgaggtaatatttgctggaccaggacaatatcgttggatgttcccattcgagggaaaaatgcataatttaaaggattattgtagaaccaatgcccatcccgaaggatcaattgctgaaggttattgcgacagtgaatgcctcacattttgttctatgtactttcgcgacattgagacaaaatttaaccaaggagacaggaatcatgatgtgagtgagagaagaataactagaatatctgttttcaaccagaatgttagatttttaaaaggagcagttgatgatgttcttagcctaactgattttgcaatgattcgatgggagcataaagcagaacttgagaggcagaatattacaaacataggaaaggaacaacaacaaagatttcacaagtggtttttgagacgtgttcaacatatgcaagttgagggttcaacagaacatatcgaatccttactaaacttggccaatggacctcagcgtgaagtgacacagtataaagttgaagtgggtgatttattagaggacattgaagatccatatcagcaagagtcacatgagtatgtcaatttagatgaaattgacattggggagacttcattgtgtagagatgacagtgaaatgatcattgttgatttgaatgctgacaagcaagggattagtgattctacattcatcaatgatagcgatgcaaaagatggtgactttggtgatgaagataatgagtttatgtattctgatgatgacgaggacaactatacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

420

Amino Acids

49.05

Weight (kDa)

4.85

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 666
AciI CCGC 1 cut(s) 280
AclWI GGATC 2 cut(s) 613, 1028
AcoI YGGCCR 1 cut(s) 963
AcsI RAATTY 1 cut(s) 680
AcuI CTGAAG 2 cut(s) 30, 636
AfaI GTAC 4 cut(s) 136, 175, 308, 659
AfiI CCNNNNNNNGG 3 cut(s) 303, 550, 551
AflIII ACRYGT 1 cut(s) 904
AjiI CACGTC 1 cut(s) 905
AjnI CCWGG 1 cut(s) 520
AjuI GAANNNNNNNTTGG 2 cut(s) 1068, 1100
AluBI AGCT 1 cut(s) 436
AluI AGCT 1 cut(s) 436
Alw26I GTCTC 4 cut(s) 668, 687, 895, 1085
AlwI GGATC 2 cut(s) 613, 1028
AoxI GGCC 1 cut(s) 963
ApeKI GCWGC 1 cut(s) 277
ApoI RAATTY 1 cut(s) 680
AspS9I GGNCC 2 cut(s) 518, 971
AsuHPI GGTGA 4 cut(s) 482, 1022, 1211, 1220
AvaII GGWCC 2 cut(s) 518, 971
BalI TGGCCA 1 cut(s) 965
BbsI GAAGAC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 1 cut(s) 289
BccI CCATC 4 cut(s) 52, 603, 810, 1190
BciT130I CCWGG 1 cut(s) 522
BclI TGATCA 1 cut(s) 1122
BcoDI GTCTC 4 cut(s) 668, 687, 895, 1085
BfaI CTAG 4 cut(s) 404, 437, 494, 729
BisI GCNGC 2 cut(s) 278, 281
BlsI GCNGC 2 cut(s) 279, 282
Bme1390I CCNGG 1 cut(s) 522
Bme18I GGWCC 2 cut(s) 518, 971
BmgBI CACGTC 1 cut(s) 905
BmgT120I GGNCC 2 cut(s) 518, 971
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 128, 1177
BpiI GAAGAC 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 975
BpuEI CTTGAG 1 cut(s) 857
BsaBI GATNNNNATC 2 cut(s) 62, 1038
BsaJI CCNNGG 1 cut(s) 688
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 550, 551
Bse3DI GCAATG 1 cut(s) 813
Bse8I GATNNNNATC 2 cut(s) 62, 1038
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 1 cut(s) 688
BseGI GGATG 2 cut(s) 543, 595
BseJI GATNNNNATC 2 cut(s) 62, 1038
BseLI CCNNNNNNNGG 3 cut(s) 303, 550, 551
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 1 cut(s) 989
BseXI GCAGC 1 cut(s) 289
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 965
BslI CCNNNNNNNGG 3 cut(s) 303, 550, 551
BsmAI GTCTC 4 cut(s) 668, 687, 895, 1085
BsmBI CGTCTC 1 cut(s) 895
BsmI GAATGC 2 cut(s) 641, 1144
BsnI GGCC 1 cut(s) 965
Bsp1407I TGTACA 1 cut(s) 173
Bsp143I GATC 3 cut(s) 605, 1033, 1122
Bsp68I TCGCGA 1 cut(s) 666
BspACI CCGC 1 cut(s) 280
BspANI GGCC 1 cut(s) 965
BspCNI CTCAG 1 cut(s) 988
BspFNI CGCG 1 cut(s) 666
BspHI TCATGA 2 cut(s) 256, 703
BspPI GGATC 2 cut(s) 613, 1028
BsrDI GCAATG 1 cut(s) 813
BsrGI TGTACA 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 688
BssMI GATC 3 cut(s) 605, 1033, 1122
BssT1I CCWWGG 1 cut(s) 688
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 3 cut(s) 632, 993, 1115
Bst6I CTCTTC 1 cut(s) 48
BstAUI TGTACA 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 788, 975
BstF5I GGATG 2 cut(s) 543, 595
BstFNI CGCG 1 cut(s) 666
BstKTI GATC 3 cut(s) 608, 1036, 1125
BstMAI GTCTC 4 cut(s) 668, 687, 895, 1085
BstMBI GATC 3 cut(s) 605, 1033, 1122
BstNI CCWGG 1 cut(s) 522
BstSCI CCNGG 1 cut(s) 520
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 1 cut(s) 289
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 1033
BstYI RGATCY 1 cut(s) 1033
BsuRI GGCC 1 cut(s) 965
BtgZI GCGATG 1 cut(s) 1200
BtrI CACGTC 1 cut(s) 905
BtsCI GGATG 2 cut(s) 543, 595
BtsIMutI CAGTG 3 cut(s) 6, 637, 1120
BtuMI TCGCGA 1 cut(s) 666
Cac8I GCNNGC 1 cut(s) 434
CciI TCATGA 2 cut(s) 256, 703
Cfr13I GGNCC 2 cut(s) 518, 971
Csp6I GTAC 4 cut(s) 135, 174, 307, 658
CviAII CATG 3 cut(s) 257, 704, 1055
CviJI RGCY 5 cut(s) 247, 283, 436, 792, 965
CviKI_1 RGCY 5 cut(s) 247, 283, 436, 792, 965
CviQI GTAC 4 cut(s) 135, 174, 307, 658
DdeI CTNAG 2 cut(s) 788, 975
DpnI GATC 3 cut(s) 607, 1035, 1124
DpnII GATC 3 cut(s) 605, 1033, 1122
DraI TTTAAA 2 cut(s) 570, 765
EaeI YGGCCR 1 cut(s) 963
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 688
Eco47I GGWCC 2 cut(s) 518, 971
Eco57I CTGAAG 2 cut(s) 30, 636
EcoRII CCWGG 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 688
EcoT22I ATGCAT 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 688
Esp3I CGTCTC 1 cut(s) 895
FaeI CATG 3 cut(s) 260, 707, 1058
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 819, 851
FatI CATG 3 cut(s) 256, 703, 1054
FauNDI CATATG 1 cut(s) 915
FbaI TGATCA 1 cut(s) 1122
Fnu4HI GCNGC 2 cut(s) 278, 281
FokI GGATG 2 cut(s) 550, 582
Fsp4HI GCNGC 2 cut(s) 278, 281
FspBI CTAG 4 cut(s) 404, 437, 494, 729
GluI GCNGC 2 cut(s) 278, 281
HaeIII GGCC 1 cut(s) 965
Hin1II CATG 3 cut(s) 260, 707, 1058
HphI GGTGA 4 cut(s) 482, 1022, 1211, 1220
Hpy166II GTNNAC 2 cut(s) 220, 366
Hpy188I TCNGA 2 cut(s) 73, 1237
Hpy188III TCNNGA 9 cut(s) 44, 158, 185, 257, 326, 373, 599, 665, 704
Hpy8I GTNNAC 2 cut(s) 220, 366
HpyAV CCTTC 4 cut(s) 5, 465, 596, 611
HpyCH4III ACNGT 3 cut(s) 632, 993, 1115
HpyCH4IV ACGT 1 cut(s) 904
HpyCH4V TGCA 7 cut(s) 79, 277, 432, 562, 806, 919, 1190
HpyF3I CTNAG 2 cut(s) 788, 975
HpySE526I ACGT 1 cut(s) 904
Hsp92II CATG 3 cut(s) 260, 707, 1058
Ksp22I TGATCA 1 cut(s) 1122
Kzo9I GATC 3 cut(s) 605, 1033, 1122
LmnI GCTCC 2 cut(s) 770, 820
LpnPI CCDG 7 cut(s) 198, 303, 501, 507, 534, 682, 761
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 2 cut(s) 128, 1177
MaeI CTAG 4 cut(s) 404, 437, 494, 729
MaeII ACGT 1 cut(s) 904
MaeIII GTNAC 3 cut(s) 985, 1050, 1199
MalI GATC 3 cut(s) 607, 1035, 1124
MboI GATC 3 cut(s) 605, 1033, 1122
MboII GAAGA 6 cut(s) 65, 189, 278, 732, 1043, 1226
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 1 cut(s) 1033
MlsI TGGCCA 1 cut(s) 965
MluNI TGGCCA 1 cut(s) 965
MlyI GAGTC 3 cut(s) 31, 469, 1058
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 9 cut(s) 322, 496, 544, 650, 834, 919, 984, 1015, 1240
Mox20I TGGCCA 1 cut(s) 965
Mph1103I ATGCAT 1 cut(s) 564
MscI TGGCCA 1 cut(s) 965
MseI TTAA 7 cut(s) 105, 210, 357, 465, 569, 684, 764
MslI CAYNNNNRTG 1 cut(s) 1059
Msp20I TGGCCA 1 cut(s) 965
MspA1I CMGCKG 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 522
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 2 cut(s) 641, 1144
MvaI CCWGG 1 cut(s) 522
MvnI CGCG 1 cut(s) 666
NdeI CATATG 1 cut(s) 915
NdeII GATC 3 cut(s) 605, 1033, 1122
NlaIII CATG 3 cut(s) 260, 707, 1058
NmuCI GTSAC 3 cut(s) 985, 1050, 1199
NruI TCGCGA 1 cut(s) 666
NsiI ATGCAT 1 cut(s) 564
PagI TCATGA 2 cut(s) 256, 703
PctI GAATGC 2 cut(s) 641, 1144
PfeI GAWTC 7 cut(s) 26, 63, 230, 700, 811, 947, 1163
PkrI GCNGC 2 cut(s) 279, 282
PleI GAGTC 3 cut(s) 31, 469, 1057
PpsI GAGTC 3 cut(s) 31, 469, 1057
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspPI GGNCC 2 cut(s) 518, 971
PsuI RGATCY 1 cut(s) 1033
RruI TCGCGA 1 cut(s) 666
RsaI GTAC 4 cut(s) 136, 175, 308, 659
RsaNI GTAC 4 cut(s) 135, 174, 307, 658
RseI CAYNNNNRTG 1 cut(s) 1059
SaqAI TTAA 7 cut(s) 105, 210, 357, 465, 569, 684, 764
SatI GCNGC 2 cut(s) 278, 281
Sau3AI GATC 3 cut(s) 605, 1033, 1122
Sau96I GGNCC 2 cut(s) 518, 971
SchI GAGTC 3 cut(s) 31, 469, 1058
ScrFI CCNGG 1 cut(s) 522
SetI ASST 9 cut(s) 322, 333, 438, 457, 495, 507, 622, 907, 976
SfaNI GCATC 2 cut(s) 128, 1177
SinI GGWCC 2 cut(s) 518, 971
SmiMI CAYNNNNRTG 1 cut(s) 1059
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
SsiI CCGC 1 cut(s) 280
SspI AATATT 4 cut(s) 152, 391, 510, 850
SspMI CTAG 4 cut(s) 404, 437, 494, 729
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 1 cut(s) 688
TaaI ACNGT 3 cut(s) 632, 993, 1115
TaiI ACGT 1 cut(s) 907
TaqI TCGA 3 cut(s) 549, 814, 945
TatI WGTACW 3 cut(s) 134, 173, 657
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 7 cut(s) 26, 63, 230, 700, 811, 947, 1163
Tru1I TTAA 7 cut(s) 105, 210, 357, 465, 569, 684, 764
Tru9I TTAA 7 cut(s) 105, 210, 357, 465, 569, 684, 764
TscAI CASTG 3 cut(s) 13, 637, 1120
TseFI GTSAC 3 cut(s) 985, 1050, 1199
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 3 cut(s) 985, 1050, 1199
TspDTI ATGAA 6 cut(s) 18, 447, 1086, 1089, 1162, 1227
TspRI CASTG 3 cut(s) 13, 637, 1120
VpaK11BI GGWCC 2 cut(s) 518, 971
XapI RAATTY 1 cut(s) 680
XspI CTAG 4 cut(s) 404, 437, 494, 729
Zsp2I ATGCAT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.