Rh5DG168200

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000178
Physical Location & Seq
Forward (+)
17892461 .. 17896313
3853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG168200.1

Sequence Viewer

Length: 1305 bp
ATGAATTTCGCAATTGCCCACATGAAGCCAGGGTCTGATTTGATACCTTGTCCGTGCATGGATTGCAATAATTTTCGTAAATATAGCCCAGACCAAGTTGAGGATCATTTATATGCAAATGGAATGGCTAAGGATTACACTAAGTGGACAGATCATGGAGAAAATGATGATGATTTTGAACTAGAAGATGATATTTTGGAATCTGAAAATGAAGAGGATGTTGATGATGTAGTTGAAATGTTAAATGATTTCCAAGCTCATGATGATAGGGAATCTCAAGAACATGTCCCTATTATGGAAGAAAATAAGTTTAGACGATTGATGAGGGATGCAGAGCAAGAATTATACCCTGGTTGTCGAAAATTCTCTAAATTATCCTTCACTAATGTTAAGTTGTTAAAGGGAGCAGTTGATGATGTTCTTAGCCTAACTGACTTTGCAAGGATTCGATGGTATGTGCTGAATAATTGTGATGAAGTACTGCCATATATAAGGGAACATAAAGCAGAACTTGAGAGGCAAAATATTCCAAACATAGAAAAAGAACAACAACAGAAGTTTCATAAATGGTTTTTGCGACGTGTTCAACAGATGCAAGTTGAGGGTTCAACAGAAGATGTTGAATCCTTACTATACTTGGCCAGTGGACCTCAACGTGAAGTGGCACGATATAGTGGGTGTATCGTCAATGGCATAAGGTTTCATACTCAGAAACGCGATGCAAATAAAAAAACTCAAAACTATGGAGTGGTAGTCAAAGGAGAGCATCGTGGTAAAAGTATCGACTTTTATGGTGTTTTGAAAGATATAATTGTGTTATCATACCTTGGAAATAATCAGGTGGTCGTTTTCAAATGTGATTGGCTGGATCTCAATGCAAGAAGGGGAATTCAAGTTGATGAGAACCAGTTTACAAGTGTTAATTTTACCAAAAAATGGTATATGAATGATCCATTTGCATTAGCATGTCAAACACAGCAGGTATACTATTTGAAAGATACAAAGCATGGTAGTAATTGGCGTGTTGTAGAAAGGTCGCAGCCTAGAGGGATGTATGATTTTACAGAAAAAGAAACTGAAGTGGGTGATTCATTAGAGGAGGTAGAAGATCCATATCAGCAAGAGTCACATGGGTATGTCGATTCAGTTGAAGTTAATGTTGGAGAGACGTCATTACATAGAGATGACATGGATATGATCGTTGTTGATTTGAATGCTAAAGATAGTGATGCAGAAGATGACGACTTTAATGATGAAGATAATGAGTTGATGTCTTATGATGATATCGAGGATGACTCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

434

Amino Acids

50.71

Weight (kDa)

4.55

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 2 - 54 5e-13 Transposase-associated domain
DUF4216 PF13952 273 - 343 1.4e-23 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1172
Acc36I ACCTGC 1 cut(s) 970
AccB7I CCANNNNNTGG 1 cut(s) 936
AccI GTMKAC 1 cut(s) 984
AccII CGCG 1 cut(s) 717
AclWI GGATC 4 cut(s) 111, 876, 944, 1103
AcoI YGGCCR 1 cut(s) 639
AcsI RAATTY 3 cut(s) 4, 362, 888
AcuI CTGAAG 1 cut(s) 1098
AcyI GRCGYC 1 cut(s) 1169
AdeI CACNNNGTG 1 cut(s) 144
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 3 cut(s) 100, 295, 936
AflIII ACRYGT 2 cut(s) 283, 580
AjiI CACGTC 1 cut(s) 581
AjnI CCWGG 2 cut(s) 28, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 1143, 1175
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 1160
AlwI GGATC 4 cut(s) 111, 876, 944, 1103
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 1 cut(s) 639
ApeKI GCWGC 1 cut(s) 1039
ApoI RAATTY 3 cut(s) 4, 362, 888
AspS9I GGNCC 1 cut(s) 647
AsuHPI GGTGA 1 cut(s) 1097
AvaII GGWCC 1 cut(s) 647
BalI TGGCCA 1 cut(s) 641
BbvI GCAGC 1 cut(s) 1051
BccI CCATC 1 cut(s) 444
BciT130I CCWGG 2 cut(s) 30, 351
BcoDI GTCTC 1 cut(s) 1160
BfaI CTAG 2 cut(s) 182, 1044
BfuAI ACCTGC 1 cut(s) 970
BisI GCNGC 1 cut(s) 1040
BlsI GCNGC 1 cut(s) 1041
BmcAI AGTACT 1 cut(s) 480
Bme1390I CCNGG 2 cut(s) 30, 351
Bme18I GGWCC 1 cut(s) 647
BmgBI CACGTC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 647
BmrFI CCNGG 2 cut(s) 30, 351
BmsI GCATC 5 cut(s) 319, 582, 709, 775, 1219
BplI GAGNNNNNCTC 1 cut(s) 1280
Bpu10I CCTNAGC 1 cut(s) 129
BpuEI CTTGAG 2 cut(s) 261, 533
BsaAI YACGTR 1 cut(s) 1302
BsaBI GATNNNNATC 1 cut(s) 1113
BsaHI GRCGYC 1 cut(s) 1169
BsaJI CCNNGG 3 cut(s) 29, 349, 826
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 295, 936
Bse1I ACTGG 2 cut(s) 642, 907
Bse8I GATNNNNATC 1 cut(s) 1113
BseBI CCWGG 2 cut(s) 30, 351
BseDI CCNNGG 3 cut(s) 29, 349, 826
BseGI GGATG 4 cut(s) 223, 334, 1056, 1297
BseJI GATNNNNATC 1 cut(s) 1113
BseLI CCNNNNNNNGG 3 cut(s) 100, 295, 936
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 2 cut(s) 642, 907
BseRI GAGGAG 1 cut(s) 1112
BseXI GCAGC 1 cut(s) 1051
Bsh1236I CGCG 1 cut(s) 717
BshFI GGCC 1 cut(s) 641
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 100, 295, 936
BsmAI GTCTC 1 cut(s) 1160
BsmBI CGTCTC 1 cut(s) 1160
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 1219
BsnI GGCC 1 cut(s) 641
Bsp143I GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 1 cut(s) 721
BspFNI CGCG 1 cut(s) 717
BspHI TCATGA 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 4 cut(s) 111, 876, 944, 1103
BsrI ACTGG 2 cut(s) 642, 907
BssECI CCNNGG 3 cut(s) 29, 349, 826
BssMI GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
BssNAI GTATAC 1 cut(s) 985
BssNI GRCGYC 1 cut(s) 1169
BssT1I CCWWGG 1 cut(s) 826
Bst1107I GTATAC 1 cut(s) 985
Bst2UI CCWGG 2 cut(s) 30, 351
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 1169
BstAPI GCANNNNNTGC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 1302
BstDEI CTNAG 4 cut(s) 129, 141, 422, 708
BstF5I GGATG 4 cut(s) 223, 334, 1056, 1297
BstFNI CGCG 1 cut(s) 717
BstKTI GATC 6 cut(s) 106, 154, 871, 952, 1111, 1200
BstMAI GTCTC 1 cut(s) 1160
BstMBI GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNI CCWGG 2 cut(s) 30, 351
BstNSI RCATGY 2 cut(s) 287, 969
BstSCI CCNGG 2 cut(s) 28, 349
BstUI CGCG 1 cut(s) 717
BstV1I GCAGC 1 cut(s) 1051
BstX2I RGATCY 2 cut(s) 868, 1108
BstYI RGATCY 2 cut(s) 868, 1108
BstZ17I GTATAC 1 cut(s) 985
BsuRI GGCC 1 cut(s) 641
BtgZI GCGATG 1 cut(s) 732
BtrI CACGTC 1 cut(s) 581
BtsCI GGATG 4 cut(s) 223, 334, 1056, 1297
BtsIMutI CAGTG 1 cut(s) 649
BveI ACCTGC 1 cut(s) 970
CaiI CAGNNNCTG 1 cut(s) 35
CciI TCATGA 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 647
Csp6I GTAC 1 cut(s) 479
CviAII CATG 9 cut(s) 22, 58, 155, 260, 284, 966, 1007, 1130, 1189
CviJI RGCY 8 cut(s) 28, 87, 128, 257, 426, 641, 865, 1042
CviKI_1 RGCY 8 cut(s) 28, 87, 128, 257, 426, 641, 865, 1042
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 4 cut(s) 129, 141, 422, 708
DpnI GATC 6 cut(s) 105, 153, 870, 951, 1110, 1199
DpnII GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
DraIII CACNNNGTG 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 826
Eco32I GATATC 1 cut(s) 1285
Eco47I GGWCC 1 cut(s) 647
Eco57I CTGAAG 1 cut(s) 1098
EcoRI GAATTC 1 cut(s) 888
EcoRII CCWGG 2 cut(s) 28, 349
EcoRV GATATC 1 cut(s) 1285
EcoT14I CCWWGG 1 cut(s) 826
ErhI CCWWGG 1 cut(s) 826
Esp3I CGTCTC 1 cut(s) 1160
FaeI CATG 9 cut(s) 25, 61, 158, 263, 287, 969, 1010, 1133, 1192
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 1 cut(s) 272
FatI CATG 9 cut(s) 21, 57, 154, 259, 283, 965, 1006, 1129, 1188
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 1 cut(s) 1040
FokI GGATG 3 cut(s) 230, 341, 1063
Fsp4HI GCNGC 1 cut(s) 1040
FspBI CTAG 2 cut(s) 182, 1044
GluI GCNGC 1 cut(s) 1040
HaeIII GGCC 1 cut(s) 641
Hin1I GRCGYC 1 cut(s) 1169
Hin1II CATG 9 cut(s) 25, 61, 158, 263, 287, 969, 1010, 1133, 1192
HinfI GANTC 8 cut(s) 200, 272, 445, 623, 1088, 1124, 1142, 1295
HphI GGTGA 1 cut(s) 1097
Hpy166II GTNNAC 4 cut(s) 147, 647, 912, 985
Hpy188I TCNGA 3 cut(s) 37, 205, 711
Hpy188III TCNNGA 2 cut(s) 260, 278
Hpy8I GTNNAC 4 cut(s) 147, 647, 912, 985
Hpy99I CGWCG 1 cut(s) 582
HpyAV CCTTC 2 cut(s) 388, 876
HpyCH4IV ACGT 4 cut(s) 580, 655, 1169, 1301
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 4 cut(s) 129, 141, 422, 708
HpySE526I ACGT 4 cut(s) 580, 655, 1169, 1301
Hsp92I GRCGYC 1 cut(s) 1169
Hsp92II CATG 9 cut(s) 25, 61, 158, 263, 287, 969, 1010, 1133, 1192
Kzo9I GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 1 cut(s) 1051
LweI GCATC 5 cut(s) 319, 582, 709, 775, 1219
MaeI CTAG 2 cut(s) 182, 1044
MaeII ACGT 4 cut(s) 580, 655, 1169, 1301
MaeIII GTNAC 1 cut(s) 1125
MalI GATC 6 cut(s) 105, 153, 870, 951, 1110, 1199
MboI GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
MboII GAAGA 7 cut(s) 197, 224, 311, 626, 1118, 1247, 1268
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 2 cut(s) 868, 1108
MlsI TGGCCA 1 cut(s) 641
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 2 cut(s) 1133, 1289
MmeI TCCRAC 1 cut(s) 1141
Mox20I TGGCCA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 641
MseI TTAA 6 cut(s) 242, 390, 398, 921, 1155, 1248
MslI CAYNNNNRTG 5 cut(s) 111, 964, 1134, 1182, 1193
Msp20I TGGCCA 1 cut(s) 641
MspR9I CCNGG 2 cut(s) 30, 351
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 1219
MvaI CCWGG 2 cut(s) 30, 351
MvnI CGCG 1 cut(s) 717
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
NlaIII CATG 9 cut(s) 25, 61, 158, 263, 287, 969, 1010, 1133, 1192
NmuCI GTSAC 1 cut(s) 1125
NspI RCATGY 2 cut(s) 287, 969
PagI TCATGA 1 cut(s) 259
PciI ACATGT 1 cut(s) 283
PctI GAATGC 1 cut(s) 1219
PfeI GAWTC 6 cut(s) 200, 272, 445, 623, 1088, 1142
PflMI CCANNNNNTGG 1 cut(s) 936
PkrI GCNGC 1 cut(s) 1041
PleI GAGTC 2 cut(s) 1132, 1289
PpsI GAGTC 2 cut(s) 1132, 1289
Ppu21I YACGTR 1 cut(s) 1302
PscI ACATGT 1 cut(s) 283
Psp6I CCWGG 2 cut(s) 28, 349
PspGI CCWGG 2 cut(s) 28, 349
PspPI GGNCC 1 cut(s) 647
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 868, 1108
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 5 cut(s) 111, 964, 1134, 1182, 1193
SaqAI TTAA 6 cut(s) 242, 390, 398, 921, 1155, 1248
SatI GCNGC 1 cut(s) 1040
Sau3AI GATC 6 cut(s) 103, 151, 868, 949, 1108, 1197
Sau96I GGNCC 1 cut(s) 647
ScaI AGTACT 1 cut(s) 480
SchI GAGTC 2 cut(s) 1133, 1289
ScrFI CCNGG 2 cut(s) 30, 351
SfaNI GCATC 5 cut(s) 319, 582, 709, 775, 1219
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 5 cut(s) 111, 964, 1134, 1182, 1193
SmlI CTYRAG 2 cut(s) 276, 512
SmoI CTYRAG 2 cut(s) 276, 512
SspI AATATT 1 cut(s) 526
SspMI CTAG 2 cut(s) 182, 1044
StyD4I CCNGG 2 cut(s) 28, 349
StyI CCWWGG 1 cut(s) 826
TaiI ACGT 4 cut(s) 583, 658, 1172, 1304
TaqI TCGA 5 cut(s) 358, 448, 783, 1140, 1287
TatI WGTACW 1 cut(s) 478
TfiI GAWTC 6 cut(s) 200, 272, 445, 623, 1088, 1142
Tru1I TTAA 6 cut(s) 242, 390, 398, 921, 1155, 1248
Tru9I TTAA 6 cut(s) 242, 390, 398, 921, 1155, 1248
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 1 cut(s) 1125
TseI GCWGC 1 cut(s) 1039
Tsp45I GTSAC 1 cut(s) 1125
TspDTI ATGAA 9 cut(s) 17, 38, 225, 489, 551, 692, 959, 1080, 1269
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 1 cut(s) 649
Van91I CCANNNNNTGG 1 cut(s) 936
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 3 cut(s) 4, 362, 888
XceI RCATGY 2 cut(s) 287, 969
XmiI GTMKAC 1 cut(s) 984
XspI CTAG 2 cut(s) 182, 1044
ZraI GACGTC 1 cut(s) 1170
ZrmI AGTACT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.