Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 176 |
| AarI | CACCTGC | 1 cut(s) | 139 |
| Acc16I | TGCGCA | 2 cut(s) | 374, 420 |
| Acc36I | ACCTGC | 2 cut(s) | 139, 292 |
| AccB1I | GGYRCC | 1 cut(s) | 482 |
| AccI | GTMKAC | 1 cut(s) | 280 |
| AciI | CCGC | 1 cut(s) | 217 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 2 cut(s) | 26, 722 |
| AcvI | CACGTG | 1 cut(s) | 748 |
| AdeI | CACNNNGTG | 1 cut(s) | 795 |
| AflIII | ACRYGT | 2 cut(s) | 328, 745 |
| AgsI | TTSAA | 5 cut(s) | 153, 207, 240, 437, 727 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 513 |
| AjiI | CACGTC | 1 cut(s) | 500 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 462, 494, 713, 745 |
| Alw26I | GTCTC | 2 cut(s) | 348, 500 |
| Ama87I | CYCGRG | 1 cut(s) | 510 |
| AoxI | GGCC | 1 cut(s) | 3 |
| ApeKI | GCWGC | 1 cut(s) | 627 |
| ApoI | RAATTY | 2 cut(s) | 26, 722 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 590, 622 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 235 |
| AspLEI | GCGC | 2 cut(s) | 375, 421 |
| AspS9I | GGNCC | 1 cut(s) | 20 |
| AsuHPI | GGTGA | 2 cut(s) | 351, 515 |
| AvaI | CYCGRG | 1 cut(s) | 510 |
| AvaII | GGWCC | 1 cut(s) | 20 |
| BaeGI | GKGCMC | 1 cut(s) | 487 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BanI | GGYRCC | 1 cut(s) | 482 |
| BbrPI | CACGTG | 1 cut(s) | 748 |
| BbvI | GCAGC | 1 cut(s) | 614 |
| BccI | CCATC | 2 cut(s) | 316, 694 |
| BceAI | ACGGC | 1 cut(s) | 572 |
| BcoDI | GTCTC | 2 cut(s) | 348, 500 |
| BfaI | CTAG | 2 cut(s) | 96, 228 |
| BfuAI | ACCTGC | 2 cut(s) | 139, 292 |
| BisI | GCNGC | 1 cut(s) | 628 |
| BlsI | GCNGC | 1 cut(s) | 629 |
| Bme18I | GGWCC | 1 cut(s) | 20 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 513 |
| BmeT110I | CYCGRG | 1 cut(s) | 510 |
| BmgBI | CACGTC | 1 cut(s) | 500 |
| BmgT120I | GGNCC | 1 cut(s) | 20 |
| BmiI | GGNNCC | 1 cut(s) | 484 |
| BmsI | GCATC | 3 cut(s) | 43, 86, 413 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 328, 360 |
| BpuEI | CTTGAG | 1 cut(s) | 429 |
| Bsa29I | ATCGAT | 1 cut(s) | 262 |
| BsaAI | YACGTR | 1 cut(s) | 748 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 295, 325 |
| Bse1I | ACTGG | 1 cut(s) | 604 |
| Bse3DI | GCAATG | 2 cut(s) | 140, 212 |
| BseCI | ATCGAT | 1 cut(s) | 262 |
| BseGI | GGATG | 3 cut(s) | 271, 454, 691 |
| BseMI | GCAATG | 2 cut(s) | 140, 212 |
| BseNI | ACTGG | 1 cut(s) | 604 |
| BseRI | GAGGAG | 1 cut(s) | 303 |
| BseSI | GKGCMC | 1 cut(s) | 487 |
| BseXI | GCAGC | 1 cut(s) | 614 |
| BshFI | GGCC | 1 cut(s) | 5 |
| BshNI | GGYRCC | 1 cut(s) | 482 |
| BshVI | ATCGAT | 1 cut(s) | 262 |
| BsiHKCI | CYCGRG | 1 cut(s) | 510 |
| BslFI | GGGAC | 1 cut(s) | 535 |
| BsmAI | GTCTC | 2 cut(s) | 348, 500 |
| BsmBI | CGTCTC | 1 cut(s) | 348 |
| BsmFI | GGGAC | 1 cut(s) | 535 |
| BsmI | GAATGC | 1 cut(s) | 235 |
| BsnI | GGCC | 1 cut(s) | 5 |
| BsoBI | CYCGRG | 1 cut(s) | 510 |
| Bsp1286I | GDGCHC | 1 cut(s) | 487 |
| Bsp143I | GATC | 3 cut(s) | 11, 263, 338 |
| BspACI | CCGC | 1 cut(s) | 217 |
| BspANI | GGCC | 1 cut(s) | 5 |
| BspDI | ATCGAT | 1 cut(s) | 262 |
| BspLI | GGNNCC | 1 cut(s) | 484 |
| BspMI | ACCTGC | 2 cut(s) | 139, 292 |
| BspT107I | GGYRCC | 1 cut(s) | 482 |
| BsrDI | GCAATG | 2 cut(s) | 140, 212 |
| BsrI | ACTGG | 1 cut(s) | 604 |
| BssMI | GATC | 3 cut(s) | 11, 263, 338 |
| Bst6I | CTCTTC | 2 cut(s) | 420, 465 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 40 |
| BstBAI | YACGTR | 1 cut(s) | 748 |
| BstC8I | GCNNGC | 2 cut(s) | 75, 402 |
| BstDEI | CTNAG | 2 cut(s) | 168, 466 |
| BstF5I | GGATG | 3 cut(s) | 271, 454, 691 |
| BstHHI | GCGC | 2 cut(s) | 375, 421 |
| BstKTI | GATC | 3 cut(s) | 14, 266, 341 |
| BstMAI | GTCTC | 2 cut(s) | 348, 500 |
| BstMBI | GATC | 3 cut(s) | 11, 263, 338 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 40, 593, 764 |
| BstNSI | RCATGY | 3 cut(s) | 138, 332, 578 |
| BstSLI | GKGCMC | 1 cut(s) | 487 |
| BstV1I | GCAGC | 1 cut(s) | 614 |
| Bsu15I | ATCGAT | 1 cut(s) | 262 |
| BsuRI | GGCC | 1 cut(s) | 5 |
| BsuTUI | ATCGAT | 1 cut(s) | 262 |
| BtrI | CACGTC | 1 cut(s) | 500 |
| BtsCI | GGATG | 3 cut(s) | 271, 454, 691 |
| BveI | ACCTGC | 2 cut(s) | 139, 292 |
| Cac8I | GCNNGC | 2 cut(s) | 75, 402 |
| CfoI | GCGC | 2 cut(s) | 375, 421 |
| Cfr13I | GGNCC | 1 cut(s) | 20 |
| ClaI | ATCGAT | 1 cut(s) | 262 |
| CviAII | CATG | 5 cut(s) | 135, 329, 422, 575, 772 |
| CviJI | RGCY | 9 cut(s) | 5, 109, 181, 210, 250, 527, 587, 627, 758 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 109, 181, 210, 250, 527, 587, 627, 758 |
| DdeI | CTNAG | 2 cut(s) | 168, 466 |
| DpnI | GATC | 3 cut(s) | 13, 265, 340 |
| DpnII | GATC | 3 cut(s) | 11, 263, 338 |
| DraIII | CACNNNGTG | 1 cut(s) | 795 |
| DriI | GACNNNNNGTC | 1 cut(s) | 513 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 2 cut(s) | 420, 465 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 513 |
| EarI | CTCTTC | 2 cut(s) | 420, 465 |
| Eco47I | GGWCC | 1 cut(s) | 20 |
| Eco72I | CACGTG | 1 cut(s) | 748 |
| Eco88I | CYCGRG | 1 cut(s) | 510 |
| EcoO109I | RGGNCCY | 1 cut(s) | 20 |
| Esp3I | CGTCTC | 1 cut(s) | 348 |
| FaeI | CATG | 5 cut(s) | 138, 332, 425, 578, 775 |
| FaqI | GGGAC | 1 cut(s) | 535 |
| FatI | CATG | 5 cut(s) | 134, 328, 421, 574, 771 |
| FauNDI | CATATG | 1 cut(s) | 369 |
| FblI | GTMKAC | 1 cut(s) | 280 |
| Fnu4HI | GCNGC | 1 cut(s) | 628 |
| FokI | GGATG | 3 cut(s) | 258, 441, 698 |
| Fsp4HI | GCNGC | 1 cut(s) | 628 |
| FspAI | RTGCGCAY | 1 cut(s) | 420 |
| FspBI | CTAG | 2 cut(s) | 96, 228 |
| FspI | TGCGCA | 2 cut(s) | 374, 420 |
| GlaI | GCGC | 2 cut(s) | 374, 420 |
| GluI | GCNGC | 1 cut(s) | 628 |
| HaeIII | GGCC | 1 cut(s) | 5 |
| HhaI | GCGC | 2 cut(s) | 375, 421 |
| Hin1II | CATG | 5 cut(s) | 138, 332, 425, 578, 775 |
| Hin6I | GCGC | 2 cut(s) | 373, 419 |
| HinP1I | GCGC | 2 cut(s) | 373, 419 |
| HincII | GTYRAC | 1 cut(s) | 281 |
| HindII | GTYRAC | 1 cut(s) | 281 |
| HinfI | GANTC | 3 cut(s) | 304, 437, 508 |
| HphI | GGTGA | 2 cut(s) | 351, 515 |
| Hpy166II | GTNNAC | 1 cut(s) | 281 |
| Hpy188III | TCNNGA | 1 cut(s) | 569 |
| Hpy8I | GTNNAC | 1 cut(s) | 281 |
| Hpy99I | CGWCG | 2 cut(s) | 282, 501 |
| HpyAV | CCTTC | 2 cut(s) | 261, 327 |
| HpyCH4IV | ACGT | 2 cut(s) | 499, 747 |
| HpyCH4V | TGCA | 5 cut(s) | 34, 134, 145, 292, 630 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 40, 593, 764 |
| HpyF3I | CTNAG | 2 cut(s) | 168, 466 |
| HpySE526I | ACGT | 2 cut(s) | 499, 747 |
| Hsp92II | CATG | 5 cut(s) | 138, 332, 425, 578, 775 |
| HspAI | GCGC | 2 cut(s) | 373, 419 |
| Kzo9I | GATC | 3 cut(s) | 11, 263, 338 |
| LmnI | GCTCC | 2 cut(s) | 593, 652 |
| LpnPI | CCDG | 5 cut(s) | 144, 297, 500, 582, 617 |
| Lsp1109I | GCAGC | 1 cut(s) | 614 |
| LweI | GCATC | 3 cut(s) | 43, 86, 413 |
| MaeI | CTAG | 2 cut(s) | 96, 228 |
| MaeII | ACGT | 2 cut(s) | 499, 747 |
| MalI | GATC | 3 cut(s) | 13, 265, 340 |
| MboI | GATC | 3 cut(s) | 11, 263, 338 |
| MboII | GAAGA | 8 cut(s) | 141, 312, 432, 437, 482, 502, 544, 792 |
| MfeI | CAATTG | 1 cut(s) | 38 |
| MhlI | GDGCHC | 1 cut(s) | 487 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 7 cut(s) | 26, 38, 186, 598, 675, 722, 728 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 2 cut(s) | 298, 502 |
| MnlI | CCTC | 8 cut(s) | 285, 324, 372, 400, 468, 506, 748, 757 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MroXI | GAANNNNTTC | 1 cut(s) | 235 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 3 cut(s) | 185, 189, 660 |
| MslI | CAYNNNNRTG | 3 cut(s) | 133, 669, 750 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MunI | CAATTG | 1 cut(s) | 38 |
| Mva1269I | GAATGC | 1 cut(s) | 235 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 40, 593, 764 |
| NdeI | CATATG | 1 cut(s) | 369 |
| NdeII | GATC | 3 cut(s) | 11, 263, 338 |
| NlaIII | CATG | 5 cut(s) | 138, 332, 425, 578, 775 |
| NlaIV | GGNNCC | 1 cut(s) | 484 |
| NsbI | TGCGCA | 2 cut(s) | 374, 420 |
| NspI | RCATGY | 3 cut(s) | 138, 332, 578 |
| PacI | TTAATTAA | 1 cut(s) | 189 |
| PaeR7I | CTCGAG | 1 cut(s) | 510 |
| PaqCI | CACCTGC | 1 cut(s) | 139 |
| PciI | ACATGT | 1 cut(s) | 328 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 712 |
| PctI | GAATGC | 1 cut(s) | 235 |
| PdmI | GAANNNNTTC | 1 cut(s) | 235 |
| PfeI | GAWTC | 1 cut(s) | 437 |
| PkrI | GCNGC | 1 cut(s) | 629 |
| PleI | GAGTC | 2 cut(s) | 298, 502 |
| PmaCI | CACGTG | 1 cut(s) | 748 |
| PmlI | CACGTG | 1 cut(s) | 748 |
| PpsI | GAGTC | 2 cut(s) | 298, 502 |
| Ppu21I | YACGTR | 1 cut(s) | 748 |
| PpuMI | RGGWCCY | 1 cut(s) | 20 |
| PscI | ACATGT | 1 cut(s) | 328 |
| PsiI | TTATAA | 1 cut(s) | 176 |
| Psp5II | RGGWCCY | 1 cut(s) | 20 |
| PspCI | CACGTG | 1 cut(s) | 748 |
| PspN4I | GGNNCC | 1 cut(s) | 484 |
| PspPI | GGNCC | 1 cut(s) | 20 |
| PspPPI | RGGWCCY | 1 cut(s) | 20 |
| PspXI | VCTCGAGB | 1 cut(s) | 510 |
| RseI | CAYNNNNRTG | 3 cut(s) | 133, 669, 750 |
| SalI | GTCGAC | 1 cut(s) | 279 |
| SaqAI | TTAA | 3 cut(s) | 185, 189, 660 |
| SatI | GCNGC | 1 cut(s) | 628 |
| Sau3AI | GATC | 3 cut(s) | 11, 263, 338 |
| Sau96I | GGNCC | 1 cut(s) | 20 |
| SchI | GAGTC | 2 cut(s) | 298, 502 |
| SduI | GDGCHC | 1 cut(s) | 487 |
| SfaNI | GCATC | 3 cut(s) | 43, 86, 413 |
| Sfr274I | CTCGAG | 1 cut(s) | 510 |
| SinI | GGWCC | 1 cut(s) | 20 |
| SlaI | CTCGAG | 1 cut(s) | 510 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 133, 669, 750 |
| SmlI | CTYRAG | 2 cut(s) | 444, 510 |
| SmoI | CTYRAG | 2 cut(s) | 444, 510 |
| Sse9I | AATT | 7 cut(s) | 26, 38, 186, 598, 675, 722, 728 |
| SsiI | CCGC | 1 cut(s) | 217 |
| SspMI | CTAG | 2 cut(s) | 96, 228 |
| TaiI | ACGT | 2 cut(s) | 502, 750 |
| TaqI | TCGA | 4 cut(s) | 262, 280, 511, 698 |
| TasI | AATT | 7 cut(s) | 26, 38, 186, 598, 675, 722, 728 |
| TfiI | GAWTC | 1 cut(s) | 437 |
| Tru1I | TTAA | 3 cut(s) | 185, 189, 660 |
| Tru9I | TTAA | 3 cut(s) | 185, 189, 660 |
| TseI | GCWGC | 1 cut(s) | 627 |
| TspDTI | ATGAA | 3 cut(s) | 438, 753, 788 |
| TspGWI | ACGGA | 1 cut(s) | 704 |
| VpaK11BI | GGWCC | 1 cut(s) | 20 |
| XapI | RAATTY | 2 cut(s) | 26, 722 |
| XceI | RCATGY | 3 cut(s) | 138, 332, 578 |
| XhoI | CTCGAG | 1 cut(s) | 510 |
| XmiI | GTMKAC | 1 cut(s) | 280 |
| XmnI | GAANNNNTTC | 1 cut(s) | 235 |
| XspI | CTAG | 2 cut(s) | 96, 228 |