Rh5BG388000

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000178
Physical Location & Seq
Reverse (-)
62069261 .. 62075318
6058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG388000.1

Sequence Viewer

Length: 810 bp
ATGATTAGGTTGCTGGGCTTTTCATACTTCTGGTTCAAGGTTCAGCTGGAGACGATAAGAAGGATTGGCTTTTTGTATTTCTGGCAAAATCAAGGGCTTCGTGAGAGTCAATTCAGCTCATTTTGGGTGAGTAGGAATCAGCAGATTTTGGATTGGCTATTGTATGAATCGTCTGCTATTATATGTCTCCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGAAGAGGCTTGGTGATGCTGGCTATAGTACCTTCAAGCTTGCCCGAACTAACAACCGTGGAGATGGAGCCAACAAGTCTCAATATCCAATTTACAAAAGAAGTCTTAAGAAGGTGGTCGTTTTCAAATGTGATTGGCTGGATCTCAATGCAAGAAGGGGAATTCAAGTTGATGAGAACCAGTTTACAAGTGTTAATTTTACCAAAAAATGGTATATGGATGATCCATTTGCATTGGCGTGTCAAACACAGCAGGTATACTATTTGAAAGATACAAAGCATGGTAGTAATTGGCGTGTTGTAGAAAGGTCGCAGCCTAGAGGGATGTATGATTTTACAGAAAAAGAAACTGAAGTGGGTGATTCATTAGAGGAGGTAGAAGATCCATATCAGCAAGAGACACATGGGTATGTCGATTCAGTTGAAGTTGATGTTGGAGAGACGTCATTACATAGAGATGACATGGATATGATCGTTGTTGATTTGAATGCTAAAGATAGTGATGCAGAAGATGGCGACTTTAATGATGAAGATAATGAGTTGATGTCTTATGATGATATCGAGGACGACTCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

269

Amino Acids

31.75

Weight (kDa)

4.51

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4216 PF13952 109 - 178 7.8e-23 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 677
Acc36I ACCTGC 1 cut(s) 475
AccB7I CCANNNNNTGG 1 cut(s) 441
AccI GTMKAC 1 cut(s) 489
AclWI GGATC 3 cut(s) 381, 449, 608
AcsI RAATTY 2 cut(s) 215, 393
AcuI CTGAAG 2 cut(s) 254, 603
AcyI GRCGYC 1 cut(s) 674
AfaI GTAC 2 cut(s) 230, 262
AfiI CCNNNNNNNGG 1 cut(s) 441
AflII CTTAAG 1 cut(s) 338
AgsI TTSAA 7 cut(s) 37, 268, 358, 398, 499, 656, 718
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AluBI AGCT 3 cut(s) 46, 117, 271
AluI AGCT 3 cut(s) 46, 117, 271
Alw26I GTCTC 5 cut(s) 44, 191, 315, 623, 665
AlwI GGATC 3 cut(s) 381, 449, 608
ApeKI GCWGC 1 cut(s) 544
ApoI RAATTY 2 cut(s) 215, 393
AsuHPI GGTGA 3 cut(s) 139, 257, 602
BbvI GCAGC 1 cut(s) 556
BccI CCATC 2 cut(s) 290, 737
BciT130I CCWGG 1 cut(s) 191
BcoDI GTCTC 5 cut(s) 44, 191, 315, 623, 665
BfaI CTAG 1 cut(s) 549
BfmI CTRYAG 1 cut(s) 256
BfrI CTTAAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 475
BisI GCNGC 1 cut(s) 545
BlsI GCNGC 1 cut(s) 546
Bme1390I CCNGG 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 2 cut(s) 238, 724
BplI GAGNNNNNCTC 1 cut(s) 785
BpmI CTGGAG 2 cut(s) 68, 173
BsaAI YACGTR 1 cut(s) 807
BsaBI GATNNNNATC 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 674
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 1 cut(s) 441
Bse1I ACTGG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 2 cut(s) 457, 561
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 1 cut(s) 441
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 1 cut(s) 617
BseXI GCAGC 1 cut(s) 556
BseYI CCCAGC 1 cut(s) 13
BslI CCNNNNNNNGG 1 cut(s) 441
BsmAI GTCTC 5 cut(s) 44, 191, 315, 623, 665
BsmBI CGTCTC 2 cut(s) 44, 665
BsmI GAATGC 1 cut(s) 724
Bsp143I GATC 4 cut(s) 373, 454, 613, 702
BspLI GGNNCC 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 3 cut(s) 381, 449, 608
BspTI CTTAAG 1 cut(s) 338
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 4 cut(s) 373, 454, 613, 702
BssNAI GTATAC 1 cut(s) 490
BssNI GRCGYC 1 cut(s) 674
Bst1107I GTATAC 1 cut(s) 490
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 674
BstAFI CTTAAG 1 cut(s) 338
BstBAI YACGTR 1 cut(s) 807
BstC8I GCNNGC 2 cut(s) 253, 273
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 457, 561
BstKTI GATC 4 cut(s) 376, 457, 616, 705
BstMAI GTCTC 5 cut(s) 44, 191, 315, 623, 665
BstMBI GATC 4 cut(s) 373, 454, 613, 702
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 556
BstX2I RGATCY 2 cut(s) 373, 613
BstYI RGATCY 2 cut(s) 373, 613
BstZ17I GTATAC 1 cut(s) 490
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 2 cut(s) 457, 561
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 253, 273
Csp6I GTAC 2 cut(s) 229, 261
CviAII CATG 3 cut(s) 512, 635, 694
CviQI GTAC 2 cut(s) 229, 261
DpnI GATC 4 cut(s) 375, 456, 615, 704
DpnII GATC 4 cut(s) 373, 454, 613, 702
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 790
Eco57I CTGAAG 2 cut(s) 254, 603
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 189
EcoRV GATATC 1 cut(s) 790
Esp3I CGTCTC 2 cut(s) 44, 665
FaeI CATG 3 cut(s) 515, 638, 697
FatI CATG 3 cut(s) 511, 634, 693
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 1 cut(s) 545
FokI GGATG 2 cut(s) 464, 568
Fsp4HI GCNGC 1 cut(s) 545
FspBI CTAG 1 cut(s) 549
GluI GCNGC 1 cut(s) 545
GsaI CCCAGC 1 cut(s) 17
GsuI CTGGAG 2 cut(s) 68, 173
Hin1I GRCGYC 1 cut(s) 674
Hin1II CATG 3 cut(s) 515, 638, 697
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 6 cut(s) 106, 136, 167, 593, 647, 800
HphI GGTGA 3 cut(s) 139, 257, 602
Hpy166II GTNNAC 2 cut(s) 417, 490
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 2 cut(s) 417, 490
HpyAV CCTTC 4 cut(s) 54, 274, 337, 381
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 2 cut(s) 674, 806
HpyCH4V TGCA 3 cut(s) 383, 464, 737
HpySE526I ACGT 2 cut(s) 674, 806
Hsp92I GRCGYC 1 cut(s) 674
Hsp92II CATG 3 cut(s) 515, 638, 697
Kzo9I GATC 4 cut(s) 373, 454, 613, 702
LmnI GCTCC 1 cut(s) 299
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 2 cut(s) 238, 724
MaeI CTAG 1 cut(s) 549
MaeII ACGT 2 cut(s) 674, 806
MalI GATC 4 cut(s) 375, 456, 615, 704
MboI GATC 4 cut(s) 373, 454, 613, 702
MboII GAAGA 5 cut(s) 224, 247, 623, 752, 773
MflI RGATCY 2 cut(s) 373, 613
MluCI AATT 6 cut(s) 110, 215, 321, 393, 427, 520
MlyI GAGTC 2 cut(s) 115, 794
MmeI TCCRAC 2 cut(s) 184, 646
MnlI CCTC 5 cut(s) 231, 545, 595, 598, 787
MseI TTAA 3 cut(s) 339, 426, 753
MslI CAYNNNNRTG 4 cut(s) 469, 639, 687, 698
MspA1I CMGCKG 1 cut(s) 46
MspCI CTTAAG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 724
MvaI CCWGG 1 cut(s) 191
NdeII GATC 4 cut(s) 373, 454, 613, 702
NlaIII CATG 3 cut(s) 515, 638, 697
NlaIV GGNNCC 1 cut(s) 301
PctI GAATGC 1 cut(s) 724
PfeI GAWTC 4 cut(s) 136, 167, 593, 647
PflMI CCANNNNNTGG 1 cut(s) 441
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 1 cut(s) 546
PleI GAGTC 2 cut(s) 114, 794
PpsI GAGTC 2 cut(s) 114, 794
Ppu21I YACGTR 1 cut(s) 807
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 301
PsuI RGATCY 2 cut(s) 373, 613
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 2 cut(s) 230, 262
RsaNI GTAC 2 cut(s) 229, 261
RseI CAYNNNNRTG 4 cut(s) 469, 639, 687, 698
SaqAI TTAA 3 cut(s) 339, 426, 753
SatI GCNGC 1 cut(s) 545
Sau3AI GATC 4 cut(s) 373, 454, 613, 702
SchI GAGTC 2 cut(s) 115, 794
ScrFI CCNGG 1 cut(s) 191
SfaNI GCATC 2 cut(s) 238, 724
SfcI CTRYAG 1 cut(s) 256
SmiMI CAYNNNNRTG 4 cut(s) 469, 639, 687, 698
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 6 cut(s) 110, 215, 321, 393, 427, 520
SspMI CTAG 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 2 cut(s) 677, 809
TaqI TCGA 2 cut(s) 645, 792
TasI AATT 6 cut(s) 110, 215, 321, 393, 427, 520
TfiI GAWTC 4 cut(s) 136, 167, 593, 647
Tru1I TTAA 3 cut(s) 339, 426, 753
Tru9I TTAA 3 cut(s) 339, 426, 753
TseI GCWGC 1 cut(s) 544
TspDTI ATGAA 5 cut(s) 12, 180, 225, 585, 774
Van91I CCANNNNNTGG 1 cut(s) 441
Vha464I CTTAAG 1 cut(s) 338
XapI RAATTY 2 cut(s) 215, 393
XmiI GTMKAC 1 cut(s) 489
XspI CTAG 1 cut(s) 549
ZraI GACGTC 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.