Rorug02G0117600

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000178
Physical Location & Seq
Reverse (-)
10005067 .. 10011113
6047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0117600.1

Sequence Viewer

Length: 2703 bp
ATGGCGGCGGAGAATCCGTTCAAGAGTATATTGAAATCGCTTGAGAAGCCGGACGGCGGCGAGTTCGGAAAGTACTACAGCTTGCCGGCTCTCAATGATCCTAGAGTCGATAAGCTACCGTACTCTATTAAGATCCTTCTCGAGTCTGCAATCCGTAACTGCGATGAGTTTCAGGTTAAGAGTAAGGATGTGGAGAAAATTATTGATTGGGAGAACAGTGCTCCCAAGCAGGTTGAGATTCCATTCAAACCTGCTAGAGTCCTTCTTCAGGATTTCACTGGGGTACCTGCTGTTGTCGATCTGGCTTGCATGCGAGATGCCATGAACAATCTCAAGGGAGATTCTAACAAGATTAACCCATTGGTACCAGTTGATCTCGTCATTGATCACTCGGTTCAAGTAGATGTGGCAAGATCAGACAATGCAGTGCAGGCAAACATGGAATTTGAGTTCAAGAGAAACAAAGAACGGTTTGGGTTTCTAAAATGGGGATCAAATGCATTCCATAACATGCTCGTTGTTCCTCCTGGATCAGGGATCGTTCATCAGGTTAATTTAGAATACCTTGGACGGGTGGTGTTCAACACAGATGGCTTGCTATATCCTGATAGTGTTGTTGGGACAGATTCACACACCACAATGATTGACGGACTAGGTGTTGCTGGTTGGGGAGTGGGTGGGATCGAAGCAGAAGCTGCGATGCTTGGCCAGCCCATGAGCATGGTCCTCCCTGGTGTGGTTGGGTTCAAGTTGTTTGGAAAACTGAGAGATGGTGTGACAGCTACTGATTTGGTTCTGACAGTAACTCAAATGCTGAGGAAGCATGGAGTTGTTGGGAAGTTTGTGGAGTTTTATGGGGAAGGCATGAGTGAATTGTCATTAGCTGACCGTGCTACTATTGCCAACATGTCTCCTGAGTATGGTGCAACCATGGGCTTCTTTCCTGTCGATCATGTCACGCTGCAATATCTAAAGCTAACTGGCAGAACTGCTGACACTGTTGCTATGATAGAATCTTATTTACGGGCTAATAAGATGTTCGTGGACTACAGTGAGCCCCAGATAGAGAGAGTATACTCTGCTAATGTTGAGCTAAAACTTGAGGATGTGGAGCCTTGCATATCAGGGCCAAAGAGGCCACATGATCGTGTTCCTCTGAAAGAAATGAAAGTGGATTGGCATGCATGCCTTGACAATAGAGTTGGATTTAAGGGTTTTGCTGTGCCAAAGGAATCTCAAAAGAAAGTTGTAGAGTTCTCATTTCATGGAATCCCTGCACAGCTTAGGCATGGAGATGTCGTAATTGCAGCTATTACCAGTTGCACAAACACCTCAAATCCTAGTGTAATGCTAGGAGCTGCCTTGGTTGCAAAGAAAGCTTGTGAATTAGGATTGGAGGTCAAGCCATGGATTAAGACTAGTCTTGCGCCAGGCTCTGGGGTTGTAACCAAATACTTGGAAAAGAGTGGGTTGCAGAAGTATCTGAATCAGCTAGGATTTAATATCGTTGGGTATGGATGCACCACATGCATTGGAAATTCAGGAGATATTGATGAAGCAGTGGCATCAGCTATTACGGAAAATGATATTGTAGCTTCAGCCGTATTATCTGGAAATAGGAATTTTGAGGGCCGTGTACATCCTTTAACACGGGCTAATTATCTTGCTTCTCCTCCCCTTGTGGTTGCTTATGCTCTTGCTGGCACGGTGGATATTGATTTTGACACTGAACCTATAGGGGTGGGAAAAGATGGAAAGAAGATATTTTTCAGGGATATATGGCCATCGAATCAAGAAGTAGCAGAGGCTGTGCAATCAAATGTGCTTCCTCAGATGTTTATGGCTACATATGAAGCAATCACCAAAGGAAATCCCATGTGGAATCAGTTATCTGTACCTTCTGGCACTCTCTATGCATGGGACCCAAAGTCCACTTATATACATGAGCCACCTTATTTTAAAGATATGACCATGTCTCCTCCCGGCGCTCATGGTGTGAAGAATGCTTACTGCTTGCTCAACTTTGGAGATAGTATTACAACTGACCACATCTCACCAGCTGGTAGCATCCACAAAGATAGTCCAGCAGCCAAATACCTTATGGAGCGTGGTGTTGATAGAAGAGACTTCAATTCTTATGGTAGTCGTCGTGGTAATGACGAAATAATGGCAAGAGGTACTTTTGCAAATATTCGCCTGGTCAATAAGTTTTTGAAAGGAGAAGTGGGCCCCAAAACAATTCATCATCCCTCAGGAGAGAAAGTTTCCGTGTTTGATGCTGCCATGAAGTACAAAAGTGAAGGGCATGACACAATCATTTTGGCTGGTGCTGAATATGGGAGTGGAAGTTCTCGTGATTGGGCTGCTAAGGGTCCAATGCTACTGGGGGTGAAGGCCGTCATAGCAAAGACATTTGAGCGCATTCACCGCAGTAATCTTGTGGGCATGGGTATCATTCCCCTATGCTTCAAGGCTGGGGAGGATACTGAAACTCTTGGATTGACCGGTGAGGAACGCTACACCATCGACCTTCCAAGCATTGTTAGCGAAATTAGACCCGGTCAGGATATCACAGTGGTGACAGACAATGGAAAGTCATTTGTATGTACGCTACGGTTTGATACAGAGGTGGAGCTGGCTTATTTTGATCATGGAGGCATTTTGCAATATGTCATCAGGAATCTGATCGGCGCAACGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

900

Amino Acids

98.38

Weight (kDa)

5.92

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aconitase PF00330 65 - 568 4.8e-168 Aconitase family (aconitate hydratase)
Aconitase_C PF00694 697 - 825 7.8e-40 Aconitase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 220, 259, 295
Acc65I GGTACC 2 cut(s) 283, 364
AccB1I GGYRCC 2 cut(s) 283, 364
AccB7I CCANNNNNTGG 1 cut(s) 1436
AccI GTMKAC 1 cut(s) 1074
AciI CCGC 4 cut(s) 5, 8, 57, 2428
AclWI GGATC 6 cut(s) 92, 127, 499, 538, 545, 689
AcoI YGGCCR 2 cut(s) 706, 1781
AcsI RAATTY 3 cut(s) 443, 1537, 1621
AcuI CTGAAG 2 cut(s) 251, 1581
AfaI GTAC 9 cut(s) 74, 122, 285, 366, 1638, 1896, 2179, 2291, 2608
AfiI CCNNNNNNNGG 7 cut(s) 56, 268, 533, 571, 736, 920, 1436
AflIII ACRYGT 1 cut(s) 906
AgeI ACCGGT 1 cut(s) 2504
AhdI GACNNNNNGTC 1 cut(s) 1927
AhlI ACTAGT 1 cut(s) 1418
AjnI CCWGG 4 cut(s) 526, 730, 1429, 2196
AleI CACNNNNGTG 1 cut(s) 2576
AloI GAACNNNNNNTCC 5 cut(s) 34, 434, 466, 2332, 2364
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 3 cut(s) 915, 1980, 2118
AlwI GGATC 6 cut(s) 92, 127, 499, 538, 545, 689
AlwNI CAGNNNCTG 4 cut(s) 695, 785, 1436, 1808
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 7 cut(s) 706, 1127, 1136, 1630, 1781, 2227, 2394
ApaI GGGCCC 1 cut(s) 2231
ApeKI GCWGC 7 cut(s) 695, 961, 1307, 1358, 2087, 2279, 2363
ApoI RAATTY 3 cut(s) 443, 1537, 1621
AsiGI ACCGGT 1 cut(s) 2504
Asp700I GAANNNNTTC 1 cut(s) 17
Asp718I GGTACC 2 cut(s) 283, 364
AspLEI GCGC 4 cut(s) 1429, 1988, 2421, 2693
AspS9I GGNCC 7 cut(s) 724, 1127, 1630, 1921, 2227, 2228, 2372
AsuC2I CCSGG 2 cut(s) 1983, 2559
AsuHPI GGTGA 6 cut(s) 1852, 2046, 2401, 2417, 2519, 2590
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 3 cut(s) 724, 1921, 2372
AxyI CCTNAGG 1 cut(s) 2251
BaeGI GKGCMC 1 cut(s) 2231
BaeI ACNNNNGTAYC 4 cut(s) 267, 300, 2613, 2646
BalI TGGCCA 2 cut(s) 708, 1783
BanI GGYRCC 2 cut(s) 283, 364
BanII GRGCYC 2 cut(s) 1059, 2231
BarI GAAGNNNNNNTAC 2 cut(s) 1991, 2023
BauI CACGAG 1 cut(s) 2352
Bbv12I GWGCWC 1 cut(s) 223
BbvCI CCTCAGC 1 cut(s) 815
BbvI GCAGC 7 cut(s) 682, 948, 1319, 1345, 2099, 2266, 2350
BccI CCATC 5 cut(s) 584, 764, 1745, 1792, 2531
BceAI ACGGC 4 cut(s) 70, 1586, 1617, 2381
BciT130I CCWGG 4 cut(s) 528, 732, 1431, 2198
BciVI GTATCC 1 cut(s) 2476
BclI TGATCA 2 cut(s) 385, 2647
BcnI CCSGG 2 cut(s) 1983, 2559
BcoDI GTCTC 3 cut(s) 915, 1980, 2118
BcuI ACTAGT 1 cut(s) 1418
BfaI CTAG 7 cut(s) 102, 255, 653, 1341, 1352, 1419, 1493
BfmI CTRYAG 3 cut(s) 76, 1048, 1734
BfoI RGCGCY 1 cut(s) 1989
BfuAI ACCTGC 3 cut(s) 220, 259, 295
BfuI GTATCC 1 cut(s) 2476
BglI GCCNNNNNGGC 1 cut(s) 1135
BisI GCNGC 9 cut(s) 6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
BlsI GCNGC 9 cut(s) 7, 59, 697, 963, 1309, 1360, 2089, 2281, 2365
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 6 cut(s) 528, 732, 1431, 1983, 2198, 2559
Bme18I GGWCC 3 cut(s) 724, 1921, 2372
BmeRI GACNNNNNGTC 1 cut(s) 1927
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 7 cut(s) 724, 1127, 1630, 1921, 2227, 2228, 2372
BmiI GGNNCC 8 cut(s) 285, 366, 1113, 1922, 1923, 2229, 2230, 2373
BmrFI CCNGG 6 cut(s) 528, 732, 1431, 1983, 2198, 2559
BmrI ACTGGG 2 cut(s) 288, 2393
BmsI GCATC 6 cut(s) 307, 690, 1508, 1574, 2076, 2266
BmuI ACTGGG 2 cut(s) 288, 2393
Bpu10I CCTNAGC 3 cut(s) 815, 1283, 2367
BpuEI CTTGAG 3 cut(s) 62, 317, 1121
BpuMI CCSGG 2 cut(s) 1983, 2559
BsaJI CCNNGG 5 cut(s) 565, 730, 930, 1362, 1406
BsaWI WCCGGW 1 cut(s) 2504
BsaXI ACNNNNNCTCC 9 cut(s) 32, 1284, 1314, 1960, 1990, 2247, 2277, 2332, 2362
Bsc4I CCNNNNNNNGG 7 cut(s) 56, 268, 533, 571, 736, 920, 1436
Bse118I RCCGGY 2 cut(s) 85, 2504
Bse1I ACTGG 5 cut(s) 283, 368, 985, 1317, 2388
Bse21I CCTNAGG 1 cut(s) 2251
BseBI CCWGG 4 cut(s) 528, 732, 1431, 2198
BseDI CCNNGG 5 cut(s) 565, 730, 930, 1362, 1406
BseGI GGATG 6 cut(s) 193, 1111, 1523, 1639, 2067, 2245
BseLI CCNNNNNNNGG 7 cut(s) 56, 268, 533, 571, 736, 920, 1436
BseMII CTCAG 5 cut(s) 755, 806, 906, 1844, 2265
BseNI ACTGG 5 cut(s) 283, 368, 985, 1317, 2388
BseRI GAGGAG 2 cut(s) 1662, 1968
BseSI GKGCMC 1 cut(s) 2231
BseXI GCAGC 7 cut(s) 682, 948, 1319, 1345, 2099, 2266, 2350
BseYI CCCAGC 1 cut(s) 2474
BsgI GTGCAG 2 cut(s) 449, 1260
BshFI GGCC 7 cut(s) 708, 1129, 1138, 1632, 1783, 2229, 2396
BshNI GGYRCC 2 cut(s) 283, 364
BshTI ACCGGT 1 cut(s) 2504
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 5 cut(s) 50, 86, 1983, 2505, 2559
BslFI GGGAC 2 cut(s) 634, 1934
BslI CCNNNNNNNGG 7 cut(s) 56, 268, 533, 571, 736, 920, 1436
BsmAI GTCTC 3 cut(s) 915, 1980, 2118
BsmFI GGGAC 2 cut(s) 634, 1934
BsmI GAATGC 3 cut(s) 500, 2008, 2421
BsnI GGCC 7 cut(s) 708, 1129, 1138, 1632, 1783, 2229, 2396
BsoBI CYCGRG 1 cut(s) 140
Bsp120I GGGCCC 1 cut(s) 2227
Bsp1286I GDGCHC 3 cut(s) 223, 1059, 2231
Bsp1407I TGTACA 1 cut(s) 1636
Bsp19I CCATGG 2 cut(s) 930, 1406
BspACI CCGC 4 cut(s) 5, 8, 57, 2428
BspANI GGCC 7 cut(s) 708, 1129, 1138, 1632, 1783, 2229, 2396
BspCNI CTCAG 5 cut(s) 756, 807, 907, 1843, 2264
BspLI GGNNCC 8 cut(s) 285, 366, 1113, 1922, 1923, 2229, 2230, 2373
BspMI ACCTGC 3 cut(s) 220, 259, 295
BspPI GGATC 6 cut(s) 92, 127, 499, 538, 545, 689
BspT107I GGYRCC 2 cut(s) 283, 364
BsrFI RCCGGY 2 cut(s) 85, 2504
BsrGI TGTACA 1 cut(s) 1636
BsrI ACTGG 5 cut(s) 283, 368, 985, 1317, 2388
BssAI RCCGGY 2 cut(s) 85, 2504
BssECI CCNNGG 5 cut(s) 565, 730, 930, 1362, 1406
BssNAI GTATAC 1 cut(s) 1075
BssSI CACGAG 1 cut(s) 2352
BssT1I CCWWGG 4 cut(s) 565, 930, 1362, 1406
Bst1107I GTATAC 1 cut(s) 1075
Bst2BI CACGAG 1 cut(s) 2352
Bst2UI CCWGG 4 cut(s) 528, 732, 1431, 2198
Bst6I CTCTTC 1 cut(s) 2116
BstAPI GCANNNNNTGC 2 cut(s) 695, 1527
BstAUI TGTACA 1 cut(s) 1636
BstDEI CTNAG 7 cut(s) 764, 815, 915, 1283, 1830, 2251, 2367
BstDSI CCRYGG 2 cut(s) 930, 1406
BstENI CCTNNNNNAGG 2 cut(s) 266, 531
BstF5I GGATG 6 cut(s) 193, 1111, 1523, 1639, 2067, 2245
BstH2I RGCGCY 1 cut(s) 1989
BstHHI GCGC 4 cut(s) 1429, 1988, 2421, 2693
BstMAI GTCTC 3 cut(s) 915, 1980, 2118
BstNI CCWGG 4 cut(s) 528, 732, 1431, 2198
BstNSI RCATGY 6 cut(s) 313, 514, 910, 1184, 1188, 1530
BstSCI CCNGG 6 cut(s) 526, 730, 1429, 1981, 2196, 2557
BstSFI CTRYAG 3 cut(s) 76, 1048, 1734
BstSLI GKGCMC 1 cut(s) 2231
BstV1I GCAGC 7 cut(s) 682, 948, 1319, 1345, 2099, 2266, 2350
BstX2I RGATCY 1 cut(s) 132
BstXI CCANNNNNNTGG 2 cut(s) 721, 1456
BstYI RGATCY 1 cut(s) 132
BstZ17I GTATAC 1 cut(s) 1075
Bsu36I CCTNAGG 1 cut(s) 2251
BsuI GTATCC 1 cut(s) 2476
BsuRI GGCC 7 cut(s) 708, 1129, 1138, 1632, 1783, 2229, 2396
BtgI CCRYGG 2 cut(s) 930, 1406
BtgZI GCGATG 2 cut(s) 177, 713
BtsCI GGATG 6 cut(s) 193, 1111, 1523, 1639, 2067, 2245
BtsI GCAGTG 2 cut(s) 432, 1566
BtsIMutI CAGTG 8 cut(s) 223, 276, 432, 996, 1057, 1566, 1725, 2580
BveI ACCTGC 3 cut(s) 220, 259, 295
CaiI CAGNNNCTG 4 cut(s) 695, 785, 1436, 1808
CfoI GCGC 4 cut(s) 1429, 1988, 2421, 2693
Cfr10I RCCGGY 2 cut(s) 85, 2504
Cfr13I GGNCC 7 cut(s) 724, 1127, 1630, 1921, 2227, 2228, 2372
Csp6I GTAC 9 cut(s) 73, 121, 284, 365, 1637, 1895, 2178, 2290, 2607
CspAI ACCGGT 1 cut(s) 2504
CspCI CAANNNNNGTGG 4 cut(s) 625, 660, 1513, 1548
CviQI GTAC 9 cut(s) 73, 121, 284, 365, 1637, 1895, 2178, 2290, 2607
DdeI CTNAG 7 cut(s) 764, 815, 915, 1283, 1830, 2251, 2367
DraI TTTAAA 1 cut(s) 1960
DriI GACNNNNNGTC 1 cut(s) 1927
EaeI YGGCCR 2 cut(s) 706, 1781
Eam1104I CTCTTC 1 cut(s) 2116
Eam1105I GACNNNNNGTC 1 cut(s) 1927
EarI CTCTTC 1 cut(s) 2116
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 4 cut(s) 565, 930, 1362, 1406
Eco24I GRGCYC 2 cut(s) 1059, 2231
Eco32I GATATC 1 cut(s) 2569
Eco47I GGWCC 3 cut(s) 724, 1921, 2372
Eco57I CTGAAG 2 cut(s) 251, 1581
Eco81I CCTNAGG 1 cut(s) 2251
Eco88I CYCGRG 1 cut(s) 140
EcoNI CCTNNNNNAGG 2 cut(s) 266, 531
EcoO109I RGGNCCY 2 cut(s) 1921, 2228
EcoRII CCWGG 4 cut(s) 526, 730, 1429, 2196
EcoRV GATATC 1 cut(s) 2569
EcoT14I CCWWGG 4 cut(s) 565, 930, 1362, 1406
EcoT22I ATGCAT 4 cut(s) 502, 1186, 1532, 1918
EcoT38I GRGCYC 2 cut(s) 1059, 2231
ErhI CCWWGG 4 cut(s) 565, 930, 1362, 1406
FalI AAGNNNNNCTT 2 cut(s) 2164, 2196
FaqI GGGAC 2 cut(s) 634, 1934
FauNDI CATATG 1 cut(s) 1849
FbaI TGATCA 2 cut(s) 385, 2647
FblI GTMKAC 1 cut(s) 1074
Fnu4HI GCNGC 9 cut(s) 6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
FokI GGATG 6 cut(s) 200, 1118, 1530, 1626, 2054, 2232
FriOI GRGCYC 2 cut(s) 1059, 2231
Fsp4HI GCNGC 9 cut(s) 6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
FspBI CTAG 7 cut(s) 102, 255, 653, 1341, 1352, 1419, 1493
GlaI GCGC 4 cut(s) 1428, 1987, 2420, 2692
GluI GCNGC 9 cut(s) 6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
GsaI CCCAGC 1 cut(s) 2478
HaeII RGCGCY 1 cut(s) 1989
HaeIII GGCC 7 cut(s) 708, 1129, 1138, 1632, 1783, 2229, 2396
HapII CCGG 5 cut(s) 50, 86, 1983, 2505, 2559
HhaI GCGC 4 cut(s) 1429, 1988, 2421, 2693
Hin6I GCGC 4 cut(s) 1427, 1986, 2419, 2691
HinP1I GCGC 4 cut(s) 1427, 1986, 2419, 2691
HindIII AAGCTT 1 cut(s) 1377
HpaII CCGG 5 cut(s) 50, 86, 1983, 2505, 2559
HphI GGTGA 6 cut(s) 1852, 2046, 2401, 2417, 2519, 2590
Hpy166II GTNNAC 4 cut(s) 1045, 1075, 1637, 1932
Hpy188I TCNGA 7 cut(s) 68, 418, 798, 1158, 1485, 1833, 2685
Hpy8I GTNNAC 4 cut(s) 1045, 1075, 1637, 1932
Hpy99I CGWCG 1 cut(s) 2151
HpyAV CCTTC 7 cut(s) 146, 272, 854, 1908, 2294, 2386, 2540
HpyF3I CTNAG 7 cut(s) 764, 815, 915, 1283, 1830, 2251, 2367
HspAI GCGC 4 cut(s) 1427, 1986, 2419, 2691
KflI GGGWCCC 1 cut(s) 1921
KpnI GGTACC 2 cut(s) 287, 368
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Ksp22I TGATCA 2 cut(s) 385, 2647
LmnI GCTCC 5 cut(s) 226, 1111, 1355, 2104, 2632
Lsp1109I GCAGC 7 cut(s) 682, 948, 1319, 1345, 2099, 2266, 2350
LweI GCATC 6 cut(s) 307, 690, 1508, 1574, 2076, 2266
MaeI CTAG 7 cut(s) 102, 255, 653, 1341, 1352, 1419, 1493
MaeIII GTNAC 6 cut(s) 155, 775, 802, 955, 1444, 2578
MboII GAAGA 4 cut(s) 257, 1771, 2011, 2133
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 3 cut(s) 223, 1059, 2231
MlsI TGGCCA 2 cut(s) 708, 1783
MluNI TGGCCA 2 cut(s) 708, 1783
MlyI GAGTC 3 cut(s) 114, 152, 267
MmeI TCCRAC 1 cut(s) 1183
Mox20I TGGCCA 2 cut(s) 708, 1783
Mph1103I ATGCAT 4 cut(s) 502, 1186, 1532, 1918
MroNI GCCGGC 1 cut(s) 85
MroXI GAANNNNTTC 1 cut(s) 17
MscI TGGCCA 2 cut(s) 708, 1783
MseI TTAA 9 cut(s) 129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
MslI CAYNNNNRTG 6 cut(s) 638, 719, 1146, 1293, 2576, 2602
Msp20I TGGCCA 2 cut(s) 708, 1783
MspA1I CMGCKG 1 cut(s) 2060
MspI CCGG 5 cut(s) 50, 86, 1983, 2505, 2559
MspR9I CCNGG 6 cut(s) 528, 732, 1431, 1983, 2198, 2559
Mva1269I GAATGC 3 cut(s) 500, 2008, 2421
MvaI CCWGG 4 cut(s) 528, 732, 1431, 2198
NaeI GCCGGC 1 cut(s) 87
NciI CCSGG 2 cut(s) 1983, 2559
NcoI CCATGG 2 cut(s) 930, 1406
NdeI CATATG 1 cut(s) 1849
NgoMIV GCCGGC 1 cut(s) 85
NlaIV GGNNCC 8 cut(s) 285, 366, 1113, 1922, 1923, 2229, 2230, 2373
NmuCI GTSAC 3 cut(s) 775, 955, 2578
NsiI ATGCAT 4 cut(s) 502, 1186, 1532, 1918
NspI RCATGY 6 cut(s) 313, 514, 910, 1184, 1188, 1530
OliI CACNNNNGTG 1 cut(s) 2576
PaeI GCATGC 3 cut(s) 313, 1184, 1188
PaeR7I CTCGAG 1 cut(s) 140
PciI ACATGT 1 cut(s) 906
PctI GAATGC 3 cut(s) 500, 2008, 2421
PdiI GCCGGC 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 17
PflFI GACNNNGTC 2 cut(s) 1972, 2559
PflMI CCANNNNNTGG 1 cut(s) 1436
PfoI TCCNGGA 1 cut(s) 526
PinAI ACCGGT 1 cut(s) 2504
PkrI GCNGC 9 cut(s) 7, 59, 697, 963, 1309, 1360, 2089, 2281, 2365
PleI GAGTC 3 cut(s) 113, 151, 266
PpsI GAGTC 3 cut(s) 113, 151, 266
PpuMI RGGWCCY 1 cut(s) 1921
PscI ACATGT 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 1921
Psp6I CCWGG 4 cut(s) 526, 730, 1429, 2196
PspFI CCCAGC 1 cut(s) 2474
PspGI CCWGG 4 cut(s) 526, 730, 1429, 2196
PspN4I GGNNCC 8 cut(s) 285, 366, 1113, 1922, 1923, 2229, 2230, 2373
PspOMI GGGCCC 1 cut(s) 2227
PspPI GGNCC 7 cut(s) 724, 1127, 1630, 1921, 2227, 2228, 2372
PspPPI RGGWCCY 1 cut(s) 1921
PstNI CAGNNNCTG 4 cut(s) 695, 785, 1436, 1808
PsuI RGATCY 1 cut(s) 132
PsyI GACNNNGTC 2 cut(s) 1972, 2559
PvuII CAGCTG 1 cut(s) 2060
RsaI GTAC 9 cut(s) 74, 122, 285, 366, 1638, 1896, 2179, 2291, 2608
RsaNI GTAC 9 cut(s) 73, 121, 284, 365, 1637, 1895, 2178, 2290, 2607
RseI CAYNNNNRTG 6 cut(s) 638, 719, 1146, 1293, 2576, 2602
SaqAI TTAA 9 cut(s) 129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
SatI GCNGC 9 cut(s) 6, 58, 696, 962, 1308, 1359, 2088, 2280, 2364
Sau96I GGNCC 7 cut(s) 724, 1127, 1630, 1921, 2227, 2228, 2372
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 3 cut(s) 114, 152, 267
ScrFI CCNGG 6 cut(s) 528, 732, 1431, 1983, 2198, 2559
SduI GDGCHC 3 cut(s) 223, 1059, 2231
SfaNI GCATC 6 cut(s) 307, 690, 1508, 1574, 2076, 2266
SfcI CTRYAG 3 cut(s) 76, 1048, 1734
SfiI GGCCNNNNNGGCC 1 cut(s) 1135
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 3 cut(s) 724, 1921, 2372
SlaI CTCGAG 1 cut(s) 140
SmiMI CAYNNNNRTG 6 cut(s) 638, 719, 1146, 1293, 2576, 2602
SmlI CTYRAG 4 cut(s) 41, 140, 332, 1100
SmoI CTYRAG 4 cut(s) 41, 140, 332, 1100
SpeI ACTAGT 1 cut(s) 1418
SphI GCATGC 3 cut(s) 313, 1184, 1188
SsiI CCGC 4 cut(s) 5, 8, 57, 2428
SspI AATATT 1 cut(s) 2191
SspMI CTAG 7 cut(s) 102, 255, 653, 1341, 1352, 1419, 1493
StyD4I CCNGG 6 cut(s) 526, 730, 1429, 1981, 2196, 2557
StyI CCWWGG 4 cut(s) 565, 930, 1362, 1406
TaqI TCGA 7 cut(s) 108, 141, 297, 684, 948, 1787, 2526
TatI WGTACW 3 cut(s) 72, 1636, 2289
TauI GCSGC 2 cut(s) 8, 60
Tru1I TTAA 9 cut(s) 129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
Tru9I TTAA 9 cut(s) 129, 177, 354, 552, 1209, 1413, 1500, 1646, 1959
TscAI CASTG 8 cut(s) 223, 283, 432, 1003, 1057, 1566, 1732, 2580
TseFI GTSAC 3 cut(s) 775, 955, 2578
TseI GCWGC 7 cut(s) 695, 961, 1307, 1358, 2087, 2279, 2363
Tsp45I GTSAC 3 cut(s) 775, 955, 2578
TspDTI ATGAA 8 cut(s) 338, 533, 1181, 1253, 1569, 1866, 2231, 2300
TspGWI ACGGA 5 cut(s) 6, 143, 663, 1592, 2257
TspRI CASTG 8 cut(s) 223, 283, 432, 1003, 1057, 1566, 1732, 2580
Tth111I GACNNNGTC 2 cut(s) 1972, 2559
Van91I CCANNNNNTGG 1 cut(s) 1436
VpaK11BI GGWCC 3 cut(s) 724, 1921, 2372
XagI CCTNNNNNAGG 2 cut(s) 266, 531
XapI RAATTY 3 cut(s) 443, 1537, 1621
XceI RCATGY 6 cut(s) 313, 514, 910, 1184, 1188, 1530
XcmI CCANNNNNNNNNTGG 1 cut(s) 2098
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 1074
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 7 cut(s) 102, 255, 653, 1341, 1352, 1419, 1493
ZrmI AGTACT 1 cut(s) 74
Zsp2I ATGCAT 4 cut(s) 502, 1186, 1532, 1918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.