Rh5CG181800

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000178
Physical Location & Seq
Forward (+)
18016252 .. 18020104
3853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG181800.1

Sequence Viewer

Length: 1164 bp
ATGAATTTCGCAATTGCCCACATGAAGCCAGGGTCTGATTTGATACCTTGTCCGTGCATGGATTGCAATAATTTTCGTAAATATAGCCCAGACCAAGTTGAGGATCATTTATATGCAAATGGAATGGCTAAGGATTACACTAAGTGGACAGATCATGGAGAAAATGATGATGATTTTGAACTAGAAGATGATATTTTGGAATCTGAAAATGAAGAGGATGTTGATGATGTAGTTGAAATGTTAAATGATTTCCAAGCTCATGATGATAGGGAATCTCAAGAACATAATGTTAAGTTGTTAAAGGGAGCAGTTGATGATGTTCTTAGCCTAACTGACTTTGCAAGGATTCGATGGGAACATAAAGCAGAACTTGAGAGGCAAAATATTCCAAACATAGAAAAAGAACAACAACAGAAGTTTCATAAATGGTTTTTGCGACGTGTTCAACAGATGCAAGTTGAGGGTTCAACAGAAGATGTTGAATCCTTACTATACTTGGCCAGTGGACCTCAACGTGAAGTGGCACGATATAGTGGGTGTATCGTCAATGGCATAAGGTTTCATACTCAGAAACGCGATGCAAATAAAAAAACTCAAAACTATGGAGTGGTAGTCAAAGGAGAGCATCGTGGTAAAAGTATCGACTTTTATGGTGTTTTGAAAGATATAATTGTGTTATCATACCTTGGAAATAATCAGGTGGTCGTTTTCAAATGTGATTGGCTGGATCTCAATGCAAGAAGGGGAATTCAAGTTGATGAGAACCAGTTTACAAGTGTTAATTTTACCAAAAAATGGTATATGAATGATCCATTTGCATTAGCATGTCAAACACAGCAGGTATACTATTTGAAAGATACAAAGCATGGTAGTAATTGGCGTGTTGTAGAAAGGTCGCAGCCTAGAGGGATGTATGATTTTACAGAAAAAGAAACTGAAGTGGGTGATTCATTAGAGGAGGTAGAAGATCCATATCAGCAAGAGTCACATGGGTATGTCGATTCAGTTGAAGTTAATGTTGGAGAGACGTCATTACATAGAGATGACATGGATATGATCGTTGTTGATTTGAATGCTAAAGATAGTGATGCAGAAGATGACGACTTTAATGATGAAGATAATGAGTTGATGTCTTATGATGATATCGAGGATGACTCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

387

Amino Acids

45.01

Weight (kDa)

4.46

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 2 - 54 4.2e-13 Transposase-associated domain
DUF4216 PF13952 226 - 296 1.2e-23 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1031
Acc36I ACCTGC 1 cut(s) 829
AccB7I CCANNNNNTGG 1 cut(s) 795
AccI GTMKAC 1 cut(s) 843
AccII CGCG 1 cut(s) 576
AclWI GGATC 4 cut(s) 111, 735, 803, 962
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 2 cut(s) 4, 747
AcuI CTGAAG 1 cut(s) 957
AcyI GRCGYC 1 cut(s) 1028
AdeI CACNNNGTG 1 cut(s) 144
AfiI CCNNNNNNNGG 2 cut(s) 100, 795
AflIII ACRYGT 1 cut(s) 439
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 1 cut(s) 28
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 1019
AlwI GGATC 4 cut(s) 111, 735, 803, 962
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 1 cut(s) 498
ApeKI GCWGC 1 cut(s) 898
ApoI RAATTY 2 cut(s) 4, 747
AspS9I GGNCC 1 cut(s) 506
AsuHPI GGTGA 1 cut(s) 956
AvaII GGWCC 1 cut(s) 506
BalI TGGCCA 1 cut(s) 500
BbvI GCAGC 1 cut(s) 910
BccI CCATC 1 cut(s) 345
BciT130I CCWGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 1019
BfaI CTAG 2 cut(s) 182, 903
BfuAI ACCTGC 1 cut(s) 829
BisI GCNGC 1 cut(s) 899
BlsI GCNGC 1 cut(s) 900
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 506
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 4 cut(s) 441, 568, 634, 1078
BplI GAGNNNNNCTC 1 cut(s) 1139
Bpu10I CCTNAGC 1 cut(s) 129
BpuEI CTTGAG 2 cut(s) 261, 392
BsaAI YACGTR 1 cut(s) 1161
BsaBI GATNNNNATC 1 cut(s) 972
BsaHI GRCGYC 1 cut(s) 1028
BsaJI CCNNGG 2 cut(s) 29, 685
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 795
Bse1I ACTGG 2 cut(s) 501, 766
Bse8I GATNNNNATC 1 cut(s) 972
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 29, 685
BseGI GGATG 3 cut(s) 223, 915, 1156
BseJI GATNNNNATC 1 cut(s) 972
BseLI CCNNNNNNNGG 2 cut(s) 100, 795
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 2 cut(s) 501, 766
BseRI GAGGAG 1 cut(s) 971
BseXI GCAGC 1 cut(s) 910
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 1 cut(s) 500
BslI CCNNNNNNNGG 2 cut(s) 100, 795
BsmAI GTCTC 1 cut(s) 1019
BsmBI CGTCTC 1 cut(s) 1019
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 1 cut(s) 500
Bsp143I GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 1 cut(s) 580
BspFNI CGCG 1 cut(s) 576
BspHI TCATGA 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 829
BspPI GGATC 4 cut(s) 111, 735, 803, 962
BsrI ACTGG 2 cut(s) 501, 766
BssECI CCNNGG 2 cut(s) 29, 685
BssMI GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
BssNAI GTATAC 1 cut(s) 844
BssNI GRCGYC 1 cut(s) 1028
BssT1I CCWWGG 1 cut(s) 685
Bst1107I GTATAC 1 cut(s) 844
Bst2UI CCWGG 1 cut(s) 30
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 1028
BstAPI GCANNNNNTGC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 1161
BstDEI CTNAG 4 cut(s) 129, 141, 323, 567
BstF5I GGATG 3 cut(s) 223, 915, 1156
BstFNI CGCG 1 cut(s) 576
BstKTI GATC 6 cut(s) 106, 154, 730, 811, 970, 1059
BstMAI GTCTC 1 cut(s) 1019
BstMBI GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 828
BstSCI CCNGG 1 cut(s) 28
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 1 cut(s) 910
BstX2I RGATCY 2 cut(s) 727, 967
BstYI RGATCY 2 cut(s) 727, 967
BstZ17I GTATAC 1 cut(s) 844
BsuRI GGCC 1 cut(s) 500
BtgZI GCGATG 1 cut(s) 591
BtrI CACGTC 1 cut(s) 440
BtsCI GGATG 3 cut(s) 223, 915, 1156
BtsIMutI CAGTG 1 cut(s) 508
BveI ACCTGC 1 cut(s) 829
CaiI CAGNNNCTG 1 cut(s) 35
CciI TCATGA 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 506
CviAII CATG 8 cut(s) 22, 58, 155, 260, 825, 866, 989, 1048
CviJI RGCY 8 cut(s) 28, 87, 128, 257, 327, 500, 724, 901
CviKI_1 RGCY 8 cut(s) 28, 87, 128, 257, 327, 500, 724, 901
DdeI CTNAG 4 cut(s) 129, 141, 323, 567
DpnI GATC 6 cut(s) 105, 153, 729, 810, 969, 1058
DpnII GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
DraIII CACNNNGTG 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 498
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 685
Eco32I GATATC 1 cut(s) 1144
Eco47I GGWCC 1 cut(s) 506
Eco57I CTGAAG 1 cut(s) 957
EcoRI GAATTC 1 cut(s) 747
EcoRII CCWGG 1 cut(s) 28
EcoRV GATATC 1 cut(s) 1144
EcoT14I CCWWGG 1 cut(s) 685
ErhI CCWWGG 1 cut(s) 685
Esp3I CGTCTC 1 cut(s) 1019
FaeI CATG 8 cut(s) 25, 61, 158, 263, 828, 869, 992, 1051
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FatI CATG 8 cut(s) 21, 57, 154, 259, 824, 865, 988, 1047
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 1 cut(s) 899
FokI GGATG 2 cut(s) 230, 922
Fsp4HI GCNGC 1 cut(s) 899
FspBI CTAG 2 cut(s) 182, 903
GluI GCNGC 1 cut(s) 899
HaeIII GGCC 1 cut(s) 500
Hin1I GRCGYC 1 cut(s) 1028
Hin1II CATG 8 cut(s) 25, 61, 158, 263, 828, 869, 992, 1051
HinfI GANTC 8 cut(s) 200, 272, 346, 482, 947, 983, 1001, 1154
HphI GGTGA 1 cut(s) 956
Hpy166II GTNNAC 4 cut(s) 147, 506, 771, 844
Hpy188I TCNGA 3 cut(s) 37, 205, 570
Hpy188III TCNNGA 2 cut(s) 260, 278
Hpy8I GTNNAC 4 cut(s) 147, 506, 771, 844
Hpy99I CGWCG 1 cut(s) 441
HpyAV CCTTC 1 cut(s) 735
HpyCH4IV ACGT 4 cut(s) 439, 514, 1028, 1160
HpyCH4V TGCA 9 cut(s) 57, 66, 116, 341, 454, 581, 737, 818, 1091
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 4 cut(s) 129, 141, 323, 567
HpySE526I ACGT 4 cut(s) 439, 514, 1028, 1160
Hsp92I GRCGYC 1 cut(s) 1028
Hsp92II CATG 8 cut(s) 25, 61, 158, 263, 828, 869, 992, 1051
Kzo9I GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
LmnI GCTCC 1 cut(s) 305
LpnPI CCDG 8 cut(s) 15, 42, 102, 514, 683, 710, 779, 824
Lsp1109I GCAGC 1 cut(s) 910
LweI GCATC 4 cut(s) 441, 568, 634, 1078
MaeI CTAG 2 cut(s) 182, 903
MaeII ACGT 4 cut(s) 439, 514, 1028, 1160
MaeIII GTNAC 1 cut(s) 984
MalI GATC 6 cut(s) 105, 153, 729, 810, 969, 1058
MboI GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
MboII GAAGA 6 cut(s) 197, 224, 485, 977, 1106, 1127
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 2 cut(s) 727, 967
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 7 cut(s) 4, 12, 70, 669, 747, 781, 874
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 2 cut(s) 992, 1148
MmeI TCCRAC 1 cut(s) 1000
MnlI CCTC 9 cut(s) 94, 208, 369, 454, 519, 899, 949, 952, 1141
Mox20I TGGCCA 1 cut(s) 500
MscI TGGCCA 1 cut(s) 500
MseI TTAA 6 cut(s) 242, 291, 299, 780, 1014, 1107
MslI CAYNNNNRTG 5 cut(s) 111, 823, 993, 1041, 1052
Msp20I TGGCCA 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 30
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
NlaIII CATG 8 cut(s) 25, 61, 158, 263, 828, 869, 992, 1051
NmuCI GTSAC 1 cut(s) 984
NspI RCATGY 1 cut(s) 828
PagI TCATGA 1 cut(s) 259
PctI GAATGC 1 cut(s) 1078
PfeI GAWTC 6 cut(s) 200, 272, 346, 482, 947, 1001
PflMI CCANNNNNTGG 1 cut(s) 795
PkrI GCNGC 1 cut(s) 900
PleI GAGTC 2 cut(s) 991, 1148
PpsI GAGTC 2 cut(s) 991, 1148
Ppu21I YACGTR 1 cut(s) 1161
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspPI GGNCC 1 cut(s) 506
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 727, 967
RseI CAYNNNNRTG 5 cut(s) 111, 823, 993, 1041, 1052
SaqAI TTAA 6 cut(s) 242, 291, 299, 780, 1014, 1107
SatI GCNGC 1 cut(s) 899
Sau3AI GATC 6 cut(s) 103, 151, 727, 808, 967, 1056
Sau96I GGNCC 1 cut(s) 506
SchI GAGTC 2 cut(s) 992, 1148
ScrFI CCNGG 1 cut(s) 30
SfaNI GCATC 4 cut(s) 441, 568, 634, 1078
SinI GGWCC 1 cut(s) 506
SmiMI CAYNNNNRTG 5 cut(s) 111, 823, 993, 1041, 1052
SmlI CTYRAG 2 cut(s) 276, 371
SmoI CTYRAG 2 cut(s) 276, 371
Sse9I AATT 7 cut(s) 4, 12, 70, 669, 747, 781, 874
SspI AATATT 1 cut(s) 385
SspMI CTAG 2 cut(s) 182, 903
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 685
TaiI ACGT 4 cut(s) 442, 517, 1031, 1163
TaqI TCGA 4 cut(s) 349, 642, 999, 1146
TasI AATT 7 cut(s) 4, 12, 70, 669, 747, 781, 874
TfiI GAWTC 6 cut(s) 200, 272, 346, 482, 947, 1001
Tru1I TTAA 6 cut(s) 242, 291, 299, 780, 1014, 1107
Tru9I TTAA 6 cut(s) 242, 291, 299, 780, 1014, 1107
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 1 cut(s) 984
TseI GCWGC 1 cut(s) 898
Tsp45I GTSAC 1 cut(s) 984
TspDTI ATGAA 8 cut(s) 17, 38, 225, 410, 551, 818, 939, 1128
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 1 cut(s) 508
Van91I CCANNNNNTGG 1 cut(s) 795
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 2 cut(s) 4, 747
XceI RCATGY 1 cut(s) 828
XmiI GTMKAC 1 cut(s) 843
XspI CTAG 2 cut(s) 182, 903
ZraI GACGTC 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.